Cleaning solution for washing blood platelet antibody detection kit microporous plate

Abstract

The invention discloses a cleaning solution for washing a blood platelet antibody detection kit microporous plate. The cleaning solution mainly comprises sodium dihydrogen phosphate, disodium hydrogenphosphate, Tween 20 and an appropriate amount of purified water and is characterized in that the pH of the cleaning solution is 6.5-7.5 at 25 DEG C, and each 200mL of purified water contains 0.2-0.8gof sodium dihydrogen phosphate, 1.0-2.5g of disodium hydrogen phosphate, 0.5-1.5mL of Tween 20 and 7-9g of sodium chloride; the cleaning solution is used for washing the microporous plate after beingdiluted by 25 times through the purified water. Compared with a conventional cleaning solution, the cleaning solution has the advantages that negative cell button lunar halo can be eliminated evidently, and the cleaning solution is simple to prepare and low in cost; the shelf life of the unopened cleaning solution at room temperature of 10-30 DEG C is four months; the shelf life of the opened cleaning solution is two months.

Description

technical field [0001] The invention relates to a washing solution for washing a platelet antibody detection kit for microporous plates. Background technique [0002] In the study, it was found that washing the microtiter plate with PBST, for many ELISA experiments, especially for the detection of antigens or antibodies in serum, the background is high, and the OD value of negative samples is even as high as 0.2-0.5 (the smaller the serum dilution factor, the higher the background ), it is difficult to distinguish from the low-concentration detection target in the sample to be tested, which reduces the sensitivity and accuracy of detection. [0003] Therefore, there is an urgent need for a washing solution that can reduce the signal-to-noise ratio of the background signal. Contents of the invention [0004] The technical problem to be solved by the present invention is to overcome the defect that the washing liquid in the prior art cannot effectively reduce the signal-to-...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect that the washing liquid in the prior art cannot effectively reduce the signal-to-noise ratio of the background signal, and to provide a washing liquid for platelet antibody detection kit microporous plate washing