A method for evaluating the growth and development toxicity of triazole pesticides using Drosophila melanogaster

Abstract

The invention provides a method for evaluating the growth and development toxicity of triazole pesticides by using Drosophila melanogaster, belonging to the technical field of toxicity research, comprising: 1) placing the third instar larvae of Drosophila melanogaster in a container containing triazole pesticides Cultivate on solid medium, count the pupation rate, eclosion rate and body weight of Drosophila melanogaster every day; 2) place the third instar larvae of Drosophila melanogaster on solid medium to cultivate, count the pupation of Drosophila melanogaster every day rate, eclosion rate and body weight; 3) when the pupation rate, eclosion rate, body weight of the Drosophila melanogaster of described step 1) are compared with the pupation rate, eclosion rate, body weight of the Drosophila melanogaster of step 2), When there is a significant difference in any item, it indicates that triazole pesticides have growth and development toxicity. The method provided by the invention can accurately evaluate the growth and development toxicity of the triazole pesticides, and provide theoretical and experimental data support for the dosage and concentration of the triazole pesticides used rationally in agriculture.

Description

Technical field[0001]The present invention is a method in which a toxic research technology is, and more particularly to a method of growing growth and development of triazole pesticides using black gamperel.Background technique[0002]The production and utilization of pesticides in recent years have risen sharply, including pesticides, acaricides, fungicides, herbicides, plant growth regulators, etc. Triazole fungicides ranked first in the global market sales in the near decade, has become the main category of global bactericides, which mainly inhibit the synthesis of sterol in the cytochrome P450 to affect the formation of the cell membrane. Using the efficient, broad spectrum and endod of triazole pesticides, it has been applied to fungal disease prevention on crops such as fruit, nuts, cereals and vegetables. However, only a small part of the pesticide acts only on the target crop, most of which enters the soil, groundwater, river and lake, etc. through the rain fog, surface runof...

AI Technical Summary

Problems solved by technology

However, after pesticide application, only a small part acts on the target crops, and most of them enter the environmental media such as soil, groundwater, rivers and lakes through rain and fog leaching, surface runoff, atmospheric deposition and food chains, etc., which are harmful to ecological security and human beings. health risks

[0003] At present, the research on the toxic effects of pesticides mainly focuses on plants, aquatic animals and mammals, and there are fewer studies on terrestrial insects.

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