A method for evaluating the growth and development toxicity of triazole pesticides using Drosophila melanogaster

A growth and development, triazole technology, applied in the field of toxicity research, can solve the problems of ecological security and human health risks, and less research on terrestrial insects

Active Publication Date: 2021-04-06
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after pesticide application, only a small part acts on the target crops, and most of them enter the environmental media such as soil, groundwater, rivers and lakes through rain and fog leaching, surface runoff, atmospheric deposition and food chains, etc., which are harmful to ecological security and human beings. health risks
[0003] At present, the research on the toxic effects of pesticides mainly focuses on plants, aquatic animals and mammals, and there are fewer studies on terrestrial insects.

Method used

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  • A method for evaluating the growth and development toxicity of triazole pesticides using Drosophila melanogaster
  • A method for evaluating the growth and development toxicity of triazole pesticides using Drosophila melanogaster
  • A method for evaluating the growth and development toxicity of triazole pesticides using Drosophila melanogaster

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]Reagent formulation: pentylol, purity 97%, purchased from Shanghai Pixia Medicine Technology Co., Ltd., dissolved with dimethyl sulfoxide. The equivalent of different concentrations of stock solutions were added to the standard medium to the designated concentration, and the equivalent dimethyl sulfoxide was added to the blank control group.

[0043]Exposure conditions: Two experimental groups were set, 5 parallel per group; blank control (no pentylol), pentylol group (10 mg / L). Each parallel experiment group was placed in a three-year-old larvae for dye culture, and 7d was cultured under standard culture conditions. An additional group was placed under standard culture conditions and was taken for Temple blue staining experiment after 12h and 30 h. The culture medium was 77.7 g of corn noodles, 32.19 g of yeast, 10.6 g agar, 0.726g CaCl231.62 g Sucrose, 63.2 g of glucose, 2G potassium sorbate and 15 ml of 5% to the hydroxybenzyl butyl carbenate solution. The cultured condition...

Embodiment 2

[0046]Reagent formulation: pentylol, purity 97%, purchased from Shanghai Pixia Medicine Technology Co., Ltd., dissolved with dimethyl sulfoxide. The equivalent of different concentrations of stock solutions were added to the standard medium to the designated concentration, and the equivalent dimethyl sulfoxide was added to the blank control group.

[0047]Exposure conditions: Two experimental groups were set, 5 parallel per group; blank control (no pentylol), pentylol group (100 mg / L). Each parallel experiment group was placed in a three-year-old larvae for dye culture, and 7d was cultured under standard culture conditions. An additional group was placed under standard culture conditions and was taken for Temple blue staining experiment after 12h and 30 h. The culture medium was 77.7 g of corn noodles, 32.19 g of yeast, 10.6 g agar, 0.726g CaCl231.62 g Sucrose, 63.2 g of glucose, 2G potassium sorbate and 15 ml of 5% to the hydroxybenzyl butyl carbenate solution. The cultured conditio...

Embodiment 3

[0050]Reagent formulation: pentylol, purity 97%, purchased from Shanghai Pixia Medicine Technology Co., Ltd., dissolved with dimethyl sulfoxide. The equivalent of different concentrations of stock solutions were added to the standard medium to the designated concentration, and the equivalent dimethyl sulfoxide was added to the blank control group.

[0051]Exposure conditions: Set 2 experimental groups, 5 parallel per group; blank control (no pentylol), pentylol group (250 mg / L). Each parallel experiment group was placed in a three-year-old larvae for dye culture, and 7d was cultured under standard culture conditions. An additional group was placed under standard culture conditions and was taken for Temple blue staining experiment after 12h and 30 h. The culture medium was 77.7 g of corn noodles, 32.19 g of yeast, 10.6 g agar, 0.726g CaCl231.62 g Sucrose, 63.2 g of glucose, 2G potassium sorbate and 15 ml of 5% to the hydroxybenzyl butyl carbenate solution. The cultured conditions were...

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Abstract

The invention provides a method for evaluating the growth and development toxicity of triazole pesticides by using Drosophila melanogaster, belonging to the technical field of toxicity research, comprising: 1) placing the third instar larvae of Drosophila melanogaster in a container containing triazole pesticides Cultivate on solid medium, count the pupation rate, eclosion rate and body weight of Drosophila melanogaster every day; 2) place the third instar larvae of Drosophila melanogaster on solid medium to cultivate, count the pupation of Drosophila melanogaster every day rate, eclosion rate and body weight; 3) when the pupation rate, eclosion rate, body weight of the Drosophila melanogaster of described step 1) are compared with the pupation rate, eclosion rate, body weight of the Drosophila melanogaster of step 2), When there is a significant difference in any item, it indicates that triazole pesticides have growth and development toxicity. The method provided by the invention can accurately evaluate the growth and development toxicity of the triazole pesticides, and provide theoretical and experimental data support for the dosage and concentration of the triazole pesticides used rationally in agriculture.

Description

Technical field[0001]The present invention is a method in which a toxic research technology is, and more particularly to a method of growing growth and development of triazole pesticides using black gamperel.Background technique[0002]The production and utilization of pesticides in recent years have risen sharply, including pesticides, acaricides, fungicides, herbicides, plant growth regulators, etc. Triazole fungicides ranked first in the global market sales in the near decade, has become the main category of global bactericides, which mainly inhibit the synthesis of sterol in the cytochrome P450 to affect the formation of the cell membrane. Using the efficient, broad spectrum and endod of triazole pesticides, it has been applied to fungal disease prevention on crops such as fruit, nuts, cereals and vegetables. However, only a small part of the pesticide acts only on the target crop, most of which enters the soil, groundwater, river and lake, etc. through the rain fog, surface runof...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K10/18A23K20/163A23K20/24A23K20/10A23K20/111
CPCA01K67/033A23K10/18A23K10/30A23K20/10A23K20/111A23K20/163A23K20/24A23K50/90
Inventor 洪喻张晴郝立翀路雯多
Owner BEIJING FORESTRY UNIVERSITY
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