Modestobacter new species and application thereof

A technology of strains and microbial strains, applied in the field of microbiology, can solve the problems of few reports on oligotrophic Bacillus strains

Active Publication Date: 2019-11-22
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although many strains of phosphate-solubilizing bacteria have been isolated in various habitats, there are few reports on the strains of the genus Bacillus adenotrophs; C

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  • Modestobacter new species and application thereof
  • Modestobacter new species and application thereof
  • Modestobacter new species and application thereof

Examples

Experimental program
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Embodiment 1

[0062] Embodiment 1, isolation and identification of bacterial strain CPCC D00004

[0063] 1. Isolation of strain CPCC D00004

[0064] Bacterial strain CPCC D00004 of the present invention is separated from Ningxia Tengger Desert soil, and concrete separation operation is as follows:

[0065] Preparation of soil suspension: Add 2g of soil sample to 18mL of sterile mixed solution containing 0.1% sodium pyrophosphate and 0.85% sodium chloride, place in a shaker at 28°C at 180 rpm, shake for 40min, and make the soil Particles are well suspended. The resulting soil suspension was diluted to 10 ﹣4 spare.

[0066] Strain isolation medium: Use M1 isolation medium with the following nutrients: Glucose 10g L -1 , yeast extract 1g·L -1 , beef extract 1g·L -1 , casein hydrolyzate 2g·L -1 , agar 15g·L -1 , ddH 2 O 1 L; pH 7.

[0067] Separation method: spread the above-mentioned diluted soil suspension on the M1 separation medium plate, and culture at 28°C for 3 weeks. Pick a s...

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Abstract

The invention discloses modestobacter new species and an application thereof, and provides Modestobacter deseti CPCC D00004 which is preserved in China General Microbiological Culture Collection Center, CGMCC with the preservation No. of CGMCC No.18004. The bacterial strain CPCC D00004 has alkaline phosphatase activity and acidic phosphatase activity, can degrade organophosphor, is functional modestobacter new species, and has broad application prospects in the respects of garbage disposal, soil remediation, control of desert, soil improvement and the like.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a new species of the genus Anotrophic Bacillus and its application. Background technique [0002] The genus Modestobacter belongs to Bacteria-Actinobacteria-Actinobacteria-Frankiales-Geodermophilaceae. [0003] In 2000, Mevs U and other scholars discovered four strains of psychrophilic actinomycetes, numbered AA-802, AA-824, AA-825 and AA-826. The 16S rRNA gene sequence information of these strains shows that they have high similarity with Geodermatophilus strains and Blastococcus strains. On the phylogeny constructed by 16S rRNA gene sequence analysis, the strain AA- 802, AA-824, AA-825, and AA-826 formed a separate clade and were adjacent to Geodermophilus and Blastococcus. Based on physiological and biochemical characteristics and cytochemical classification indicators, the genus Modestobacter was established, and the model species was Modestobacter multiseptatus, with strain A...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/16C05F11/08C09K17/14C12R1/01C09K105/00
CPCC05F11/08C09K17/14C09K2105/00C12N9/16C12Y301/03001C12Y301/03002C12N1/205C12R2001/01
Inventor 张玉琴姜竹鸣王浩张涛石云雷邓阳刘红宇余利岩
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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