Modestobacter new species and application thereof
A technology of strains and microbial strains, applied in the field of microbiology, can solve the problems of few reports on oligotrophic Bacillus strains
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[0062] Embodiment 1, isolation and identification of bacterial strain CPCC D00004
[0063] 1. Isolation of strain CPCC D00004
[0064] Bacterial strain CPCC D00004 of the present invention is separated from Ningxia Tengger Desert soil, and concrete separation operation is as follows:
[0065] Preparation of soil suspension: Add 2g of soil sample to 18mL of sterile mixed solution containing 0.1% sodium pyrophosphate and 0.85% sodium chloride, place in a shaker at 28°C at 180 rpm, shake for 40min, and make the soil Particles are well suspended. The resulting soil suspension was diluted to 10 ﹣4 spare.
[0066] Strain isolation medium: Use M1 isolation medium with the following nutrients: Glucose 10g L -1 , yeast extract 1g·L -1 , beef extract 1g·L -1 , casein hydrolyzate 2g·L -1 , agar 15g·L -1 , ddH 2 O 1 L; pH 7.
[0067] Separation method: spread the above-mentioned diluted soil suspension on the M1 separation medium plate, and culture at 28°C for 3 weeks. Pick a s...
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