Construction method and application of synovial sarcoma xenograft mouse model with sound immunity

Abstract

The invention provides a method for constructing a synovial sarcoma xenograft mouse model with sound immunity. The method comprises the steps of culture of a P0 generation, wherein firstly, fresh tumor tissue masses of a patient with a synovial sarcoma are collected and then transplanted into the body of a mouse with immune deficiency under a sterile condition; a mouse with normal immunity is subjected to ciclosporin pretreatment; culture of Px generations, wherein tumor tissue masses growing in the body of a mouse of any generation from P0-P<x-1> are taken to be inoculated into the body of amouse subjected to the ciclosporin pretreatment to form a Px-generation mouse; culture of Py generations, wherein synovial sarcoma tumor tissue masses growing in the body of a mouse of any generationfrom Px-P<y-1> are taken to be inoculated into the body of the mouse with the normal immunity until tumor tissue grows in the body of the mouse with the normal immunity, and the culture of the Py generations is completed. The method solves the problem that a traditional PDX model has no immune system, provides an in-vivo model for a tumor immunotherapy, and broadens the application range of the PDX model.

Description

technical field [0001] The invention relates to the field of establishing a tumor transplantation mouse model, in particular to a construction method and application of an immune-sound synovial sarcoma xenograft mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcomas [1]. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-ha...

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Problems solved by technology

However, this method is mainly due to the fact that the immune response caused by the initial antigen stimulation of normal immune mice is generally 7 to 10 days, while the cell-mediated immune response to the allograft is obvious on the 9th to 12th day,

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