A strain of Auricularia cereus that promotes the germination of medicinal dendrobium seeds to form seedlings and its application

A technology for dendrobium and seeds is applied in the field of applied microorganisms, which can solve the problems of cumbersome, complex effective fungal screening and separation, and achieve the effects of simple process, simple and convenient separation method, and high survival rate in the field.

Active Publication Date: 2021-02-12
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, effective fungi are mostly obtained by isolating from the roots of wild plants of Orchidaceae, but because there are a large number of endophytic fungi with unknown functions in the roots of Orchidaceae, this makes the screening and isolation of effective fungi for seed germination complicated and cumbersome.

Method used

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  • A strain of Auricularia cereus that promotes the germination of medicinal dendrobium seeds to form seedlings and its application
  • A strain of Auricularia cereus that promotes the germination of medicinal dendrobium seeds to form seedlings and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Isolation of Sebacinales sp. LQ strain

[0039] (1) Acquisition of protocorms: In September 2018, 24 protocorms of Dendrobium candidum and 3 protocorms of Dendrobium curved stems that germinated naturally were collected in the original habitats of Dendrobium candidum and Dendrobium curved stem in Luotian, Chongqing, and wrapped with moist moss bring back;

[0040] (2) Induction, separation, purification and preservation of symbiotic fungi in protocorms: take out the germinated protocorms of Dendrobium officinale and / or protocorms of Dendrobium curved stems, put them into sterilized beakers filled with 1% sodium hypochlorite (NaClO) solution respectively, Gently shake the beaker, take it out with sterilized blunt-tipped tweezers after 5 minutes, and rinse it with sterile water for 3 to 4 times; cut the protocorm of Dendrobium candidum and / or the protocorm of Dendrobium candidum with a sterile blade on an ultra-clean workbench. Cut into two halves, place them ...

Embodiment 2

[0047] Embodiment 2: Experiment of the effectiveness of promoting the symbiotic germination of Dendrobium candidum LQ bacterial strain:

[0048] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:

[0049] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4g L -1 Oat flour and 8g·L -1 Agar, the pH of the medium is 5.6-5.8; prepare 360 ​​plates of oat medium for sterilization, and there are 6 treatment groups in total;

[0050] (2) Take out the Sebacinales sp.LQ which is stored in the slant of the test tube at a temperature of 4°C, inoculate it on the PDA medium, then place it in an artificial climate box, and cultivate it at a temperature of 25±2°C Activation until the fungal hyphae cover the petr...

Embodiment 3

[0059] Embodiment 3: Experiment of the effectiveness of promoting the symbiotic germination of Dendrobium bentrobii by the LQ strain of O. cereus:

[0060] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:

[0061] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4g L -1 Oat flour and 8g·L -1 Agar, the pH of the medium is 5.6-5.8; prepare 360 ​​plates of oat medium for sterilization, and prepare 30 plates for each experiment, 12 treatments in total;

[0062] (2) Take out the Sebacinales sp.LQ which is stored in the slant of the test tube at a temperature of 4°C, inoculate it on the PDA medium, then place it in an artificial climate box, and cultivate it at a temperature of 25±2°C Activation unt...

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Abstract

The invention discloses a Sebacinales sp.LQ which promotes the germination of medicinal dendrobium seeds to form seedlings. It has been preserved in the China Center for Type Culture Collection with the preservation number CCTCC NO:M 2019744. The invention provides the morphology of the strain and its physiological and biochemical characteristics. According to the results of morphological, physiological and biochemical, nucleotide sequence and phylogenetic analysis, Sebacinales sp.LQ is a fungus belonging to the order Auricles. Strong specificity, can be used to inoculate the seeds of Dendrobium officinale and Dendrobium bentulum respectively with the fungus strain of Auricularia cereus to obtain high-quality symbiotic seedlings, realize the efficient cultivation of symbiotic germination seedlings, and can be used for seedling cultivation in nurseries or direct seeding of trunk seeds in the field. Because tissue culture has the advantages of low cost, high survival rate in the field, and no tissue degradation, it has the application value of replacing tissue culture to obtain symbiotic seedlings.

Description

technical field [0001] The invention relates to a fungus fungus cereus for promoting the germination of medicinal dendrobium seeds to form seedlings and an application thereof, which belongs to the field of applied microorganisms. Background technique [0002] Dendrobium officinale (Dendrobium officinale) and Dendrobium flexicaule (Dendrobium flexicaule), as traditional precious Chinese medicinal materials in my country, have extremely high medicinal value and have been used for more than 2,000 years. Reviving Grass". In the past, wild dendrobium was mainly collected. Due to the depletion of wild resources, the dendrobium industry has been dominated by intensive cultivation in greenhouses since the 1990s. After nearly 30 years of development, such an industrial model has huge investment in infrastructure and high requirements for planting technology. , Product safety and authenticity have been questioned. At present, the entire medicinal Dendrobium industry is in a transiti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G22/25C12R1/645
CPCA01G22/25C12N1/145C12R2001/645
Inventor 高江云王新菊
Owner YUNNAN UNIV
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