Fungus of the family Glycobacteriaceae and its application in promoting seed germination of Paphiopedilum albicans to form seedlings

A technology of film bacteria and fungi, which is applied in the field of applied microorganisms, can solve the problems of complex and cumbersome screening and separation of effective fungi for seed germination, and uncertainty about whether the effective symbiotic fungi are the same, and achieve simple and convenient separation methods, simple processes, and no organization. The effect of the degradation problem

Active Publication Date: 2021-12-17
YUNNAN UNIV
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Problems solved by technology

At present, effective fungi are mostly obtained by isolating from the roots of wild plants of Orchidaceae, but because there are a large number of endophytic fungi with unknown functions in the roots of Orchidaceae, this makes the screening and isolation of effective fungi for seed germination complicated and cumbersome. , meanwhile, whether the fungi in the roots of different orchids are the same as the effective symbiotic fungi at the seed germination stage is still uncertain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0063] Example 2: Experiment on the effectiveness of promoting the symbiotic germination of P. albicans seeds by the GYBQ02 strain of the Glycobacteriaceae fungus

[0064] The symbiotic germination experiment between seeds and fungi in the medium was used to detect whether the isolated fungi had a promoting effect on the seed germination stage and to compare the differences in the promoting effects of different fungi on the seed germination stage:

[0065] (1) Prepare symbiotic germination medium: the symbiotic germination medium is oat agar medium, including 4 g·L -1 oat flour and 8 g·L -1 Agar, the pH of the medium is 5.6~5.8; prepare 100 plates of oat medium for sterilization, and each experimental treatment repeats 30 plates, a total of 3 treatments;

[0066] (2) Take out the fungus strain Tulasnellaceae GYBQ02 stored in the slant of the test tube at a temperature of 4°C, inoculate it on the PDA medium, then place it in an artificial climate box, and cultivate and activat...

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Abstract

The present invention relates to a fungus of the family Glycobacteriaceae and its application for promoting the germination of Paphiopedilum albicans seeds to form seedlings. The fungus is a strain of the family Glycobacteriaceae, Tulasnellaceae sp.GYBQ02, and its preservation number in the China Typical Culture Collection Center is: CCTCC NO: M 2020130. The invention provides the morphology and physiological and biochemical characteristics of the bacterial strain. According to the results of morphological, physiological, biochemical and nucleotide sequence analysis, Tulasnellaceae GYBQ02 belongs to a fungus belonging to the family Colloidalaceae, and this strain can be used to promote the germination of P. The strain is inoculated with the seeds of Papudendron alba to obtain high-quality symbiotic seedlings, and realizes the efficient cultivation of seedlings for symbiotic germination. It can be used for seedling cultivation in nurseries or field fungal seed dressing and live broadcast. Compared with tissue culture, it has low cost, high survival rate in field cultivation, and no organization. Degradation problems and other advantages, laying the foundation for the development of imitation ecological cultivation, species regression and population reconstruction of P.

Description

technical field [0001] The invention relates to a fungus of the family Glycobacteriaceae and the application thereof for promoting the germination of Paphiopedilum japonica seeds to form seedlings, and belongs to the field of applied microorganisms. Background technique [0002] Paphiopedilum alba ( Paphiopedilum spicerianum ), was first found in Assam in northern India, and later in northeastern India along the border with Myanmar. The sepals in the flowers are large and white, like a white flag, the upper part is curved forward into an arch, and the pseudostamens are ear-shaped and heart-shaped, which has high ornamental and breeding value. In 2006, in Pu'er City, Yunnan Province, my country, the wild distribution of P. albicans was discovered for the first time, and it is also the only confirmed wild distribution point in my country. There are only more than 20 plants in the original population. This species grows on the steep bank of a small river ditch at the entrance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C12R1/645
CPCA01N63/30C12R2001/645C12N1/145
Inventor 高江云杨文科王新菊
Owner YUNNAN UNIV
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