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Marker, identification method, detection kit and application of fetal trophoblastic cells

A trophoblast cell and detection kit technology, applied in the field of fetal trophoblast cell markers, can solve the problems of low accuracy and poor sensitivity of fetal trophoblast cell identification, etc.

Pending Publication Date: 2020-05-08
HANGZHOU JUNHUI BIOTECHNOLOGY CO LTD
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a fetal trophoblast cell marker, identification method, detection kit and application to solve the current problems of poor sensitivity and low accuracy in the identification of fetal trophoblast cells

Method used

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  • Marker, identification method, detection kit and application of fetal trophoblastic cells
  • Marker, identification method, detection kit and application of fetal trophoblastic cells
  • Marker, identification method, detection kit and application of fetal trophoblastic cells

Examples

Experimental program
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Effect test

Embodiment

[0053] Identification and analysis of fetal cells in cervical mucus for prenatal diagnosis

[0054] This embodiment includes the following steps:

[0055] (1) Use a sterile cell brush to gently brush the cervical mucus of 5 women in the first trimester (6 weeks to 8 weeks of pregnancy) (the patient has known information), rotate the brush about 10 times, and store the mucus in 2ml of the cell brush In Hank's Balanced Salt Solution (HBSS), they were coded as samples No. 1-5, and placed in a 4-degree ice box for delivery to the laboratory.

[0056] (2) Take out the cell brush, put it into 0.25% trypsin (containing chelating agent) and incubate at 37°C for 3-5 minutes to digest the mucus, and observe the cell dispersion under a microscope. When the dispersibility of the single cells was better, the trypsin digestion was terminated with fetal bovine serum (FBS).

[0057] (3) The suspension obtained in the previous step was centrifuged at 400 g for 5 minutes, and washed 3 times w...

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Abstract

The invention belongs to the technical field of biological cell identification, and particularly relates to a marker, an identification method, a detection kit and application of fetal trophoblastic cells. According to the identification method, a sample containing fetal trophoblastic cells is dyed with a fluorescently labeled HK2 antibody substance, then fluorescence detection is carried out, andHK2 positive cells are target cells. The kit comprises a fluorescently labeled HK2 antibody substance and a cell nucleus coloring agent. According to the technical scheme provided by the invention, fetal trophoblastic cells can be sensitively and reliably detected in a cervical mucus sample or a blood sample of a woman at an early pregnancy stage, and particularly, the kit can be used for prenatal diagnosis at the early pregnancy stage.

Description

technical field [0001] The invention belongs to the technical field of biological cell identification, and in particular relates to a marker, identification method, detection kit and application of fetal trophoblast cells. Background technique [0002] At present, there is no good treatment for genetic diseases of chromosomal abnormalities. Therefore, timely detection of abnormalities in fetal genetic information through prenatal diagnosis in the first trimester and active intervention can effectively reduce the rate of birth defects. Currently, prenatal diagnosis methods used in clinical practice are mainly invasive examinations, such as chorionic villi biopsy, amniocentesis, fetal cord blood testing, etc. However, such invasive examinations have certain risks of infection and miscarriage. In addition, preimplantation prenatal diagnosis can be carried out for test-tube babies, but it is only applicable to test-tube babies, and the technical requirements are very high. Non...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56966G01N2800/385C12Q1/68G01N1/30G01N2333/91215G01N33/573G01N2800/368G01N2800/387G01N33/582G01N33/6854C01F1/00
Inventor 施奇惠王春英
Owner HANGZHOU JUNHUI BIOTECHNOLOGY CO LTD
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