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Hypoxia marker molecule chl1 and its application

A CHL1 and marker technology, applied in the field of biomedical materials, to achieve sensitive and reliable detection

Inactive Publication Date: 2016-03-30
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mice lacking the CHL1 gene can survive and reproduce normally, but have certain defects in neurite development and adult learning and memory [13]

Method used

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  • Hypoxia marker molecule chl1 and its application
  • Hypoxia marker molecule chl1 and its application
  • Hypoxia marker molecule chl1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 mouse hypoxia experiment

[0018] (1) Acute hypoxia

[0019] Forty C57 mice with an average weight of 22-24 g (provided by the Animal Center of the Academy of Military Medical Sciences) were divided into two groups, 20 in each group, which were the control group and the acute hypoxia group. Experimental animals were treated in normoxic and hypoxic chambers (given 5000 meters of hypobaric hypoxia) for 24 hours. The hypoxic chamber rose to 5000 meters at a constant speed of 20 m / s, and dropped to normal oxygen at a constant speed of 20 m / s after the experiment. Immediately after the end of the hypoxic treatment, the eyeballs were removed to collect blood for the detection of CHL1.

[0020] (2) Chronic hypoxia

[0021] Sixty C57 mice with an average body weight of 22-24 g (provided by the Animal Center of the Academy of Military Medical Sciences) were divided into 3 groups, 20 in each group, which were the control group, the chronic hypoxic 14-day group, an...

Embodiment 2

[0044] Embodiment 2 people's hypoxic experiment

[0045](1) Simulated plateau hypoxia: 10 volunteers were randomly selected to enter the hypoxic chamber, and blood was collected from veins in hypoxic environments in normoxia, simulated altitude 3600m, and altitude 4800m, respectively, for the detection of CHL1.

[0046] (2) Extraction of plasma

[0047] Put the collected blood into a test tube containing an anticoagulant (dipotassium ethylenediaminetetraacetic acid), mix it, and centrifuge it at 3000rpm for 10min to separate it from the blood cells. The supernatant is obtained as plasma. Plasma was stored at -80°C for later use.

[0048] (3) CH1 content in plasma extracted by ELISA

[0049] Reagent:

[0050] 1. Carbonate coating solution: 3.03gNa 2 CO 3 , 6.0g NaHCO 3 , dissolved in 900ml double distilled water, adjust the pH to 9.6, and dilute to 1L;

[0051] 2. PBS: 1.16gNa 2 HPO 4 , 0.1gKCl, 0.1gK 3 PO 4 , 4.0gNaCl, dissolved in 500mL distilled water, adjust the ...

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Abstract

The invention discloses a marker molecule CHL1 for low oxygen, and an application thereof, and belongs to the technical field of biomedical materials. The marker molecule is a cell adhesion molecule CHLl, and the protein sequence of the marker molecule is shown as SEQ ID No. 1 or SEQ ID No. 2. The CHL1 can be used as the marker molecule for the low oxygen. An antibody and an enzyme-labeled second antibody which are prepared by the marker molecule by an immunological method can detect whether an animal or person is in the low oxygen state or not by using an enzyme-linked immunoassay method, with reliable detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a hypoxia marker molecule CHL1 and its application. Background technique [0002] Environmental hypoxic injury has always been a common problem faced by plateau medicine, aerospace medicine, sports medicine and other disciplines, and it is also an important field of physiology research in special environments [1，2] . Hypoxia can cause dysfunction of major nervous, respiratory and circulatory systems, and in severe cases, it can lead to respiratory failure, which is life-threatening [3] . Hypoxia is also a pathophysiological process shared by many diseases such as myocardial infarction, cerebral infarction, atherosclerosis and other cardiovascular and cerebrovascular diseases. In addition, hypoxia is also one of the important characteristics of the microenvironment of solid tumors. An important cause of chemotherapy resistance and poor prognosis [4] . ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535
Inventor 黄欣朱玲玲范明吴奎武赵彤吴丽颖
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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