Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of ligustilide in preparing medicine for alleviating microglial cell damage, preventing and treating cerebral arterial thrombosis or repairing cerebral injury

A technology of ischemic stroke and microglia, which is applied in the field of biomedicine to achieve the effect of repairing brain damage, reducing microglial damage, and preventing ischemic stroke

Inactive Publication Date: 2020-05-29
SUN YAT SEN UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports and records about the use of ligustilide in reducing microglial cell damage in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of ligustilide in preparing medicine for alleviating microglial cell damage, preventing and treating cerebral arterial thrombosis or repairing cerebral injury
  • Application of ligustilide in preparing medicine for alleviating microglial cell damage, preventing and treating cerebral arterial thrombosis or repairing cerebral injury
  • Application of ligustilide in preparing medicine for alleviating microglial cell damage, preventing and treating cerebral arterial thrombosis or repairing cerebral injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Cell culture protocol:

[0030] BV-2 mouse microglial cells were purchased from China Type Culture Collection Center (Wuhan University Collection Center) and cultured in RPMI1640 medium containing 10% by volume of FBS, 100U / ml double antibody, and 11.1mmol / L glucose cultivated in. The culture environment is a constant temperature of 37°C and 5% carbon dioxide. The culture medium was changed every 2 days, and the cells were subcultured when the confluence reached 80-90%.

[0031] 2. Cell experiment grouping scheme:

[0032] BV-2 microglial cells were divided into 3 groups: control group (control), glucose and oxygen deprivation / reoxygenation group (OGD / R), glucose and oxygen deprivation / reoxygenation + ligustilide group (OGD / R+ LIG). The cells in the Control group were cultured normally; the cells in the OGD / R group and OGD / R+LIG group were replaced with sugar-free medium and placed in an anaerobic jar (0.2% O 2 +4.8%CO 2 +95%N 2 ) after 3 hours of glucose and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of ligustilide in preparing a medicine for alleviating microglial cell damage, preventing and treating cerebral arterial thrombosis or repairing cerebral injury andbelongs to the technical field of biomedicines. Ligustilide can be adopted to alleviate microglial cell damage by inhibiting microglial cell pyroptosis and / or inhibiting activation of NLRP3 inflammasome, and furthermore, purposes of preventing and treating cerebral arterial thrombosis and repairing cerebral injury can be achieved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of ligustilide in the preparation of drugs for alleviating microglial cell damage, preventing and treating ischemic stroke or repairing brain damage. Background technique [0002] Microglia are common immune cells in the central nervous system (central nervous system, CNS). Once the central nervous system is invaded by endogenous or exogenous factors, microglia will be rapidly activated and gather to the lesion and its surroundings, while secreting and (or) releasing various factors. In the central nervous system, the NLRP3 inflammasome is mainly expressed in microglia. After silencing the NLRP3 gene, it can reduce the release of pro-inflammatory cytokines and reduce inflammatory responses and tissue damage. Over the years, the clinical treatment of CNS diseases has mostly focused on protecting neurons, and there are few studies on the mechanisms of other imm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/365A61P9/10A61P25/00
CPCA61K31/365A61P9/10A61P25/00
Inventor 谭红梅胡佳
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com