A method and system for estimating the age of zebrafish juveniles

Abstract

The present invention relates to a method and system for estimating the age of juvenile zebrafish, comprising: acquiring an image, performing grayscale, binarization, and closed operation processing; rotating the contour of juvenile zebrafish anticlockwise by θ to obtain a new contour; starting from the left end of the new contour Take the width at the preset width value, if the width at the preset width value is less than the preset threshold, the juvenile fish is older than 3 days; compare the first preset length on the left side of the new outline with the first preset length on the right side of the new outline. Set the width at the length value. If the width on the left side is greater than the width on the right side, the fish head will face to the left; otherwise, the fish head will face to the right, and the new contour image will be flipped; take the first preset length value and the second preset length value from the left of the new contour The two positions of , connect the points on the upper and lower sides of the contour; calculate the distance from the point between the two positions on the contour to the two connecting lines, mark the point with the largest inward distance, and finally determine the age of the zebrafish larvae. The above method in the present invention can accurately determine the age of zebrafish larvae.

Description

technical field [0001] The invention relates to the field of image processing, in particular to a method and system for estimating the age of juvenile zebrafish. Background technique [0002] Zebrafish has become one of the most important vertebrate model animals. Its advantages include extremely strong reproductive ability, high genetic similarity with human beings, fast growth, and transparency from embryo to juvenile stage, so it is easy to observe its physical development and other characteristics. Therefore, zebrafish has extremely important research value in the study of vertebrate developmental biology. [0003] Suctioning zebrafish larvae with a pipette is a relatively common immobilization method in micromanipulation of zebrafish larvae, and the suction pump is required for different morphologies of larval fish within 1 to 3 days after hatching. Set to different pressures, inappropriate pressure may cause suction failure or even damage to zebrafish larvae. It is ...

AI Technical Summary

Problems solved by technology

[0003] In the zebrafish larval micromanipulation system, using a pipette to suck zebrafish larvae is a relatively common immobilization method, and the suction pump is required for different morphologies of larval fish within 1 to 3 days after hatching. Set to different pressures, inappropriate pressure may cause suction failure or even damage to zebrafish larvae

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