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Preparation method of bispecific antibody NK cells, and cells and application thereof

A bispecific antibody and NK cell technology, applied in the field of cell culture, can solve the problems of poor phenotype of NK cells, unsuitable for practical application, and low purity

Pending Publication Date: 2020-10-27
中科宝承生物医学科技有限公司
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Problems solved by technology

[0006] This application provides a bispecific antibody NK cell preparation method and its cells and applications to solve the problems that NK cells have poor phenotype, low purity and cannot meet the needs of practical applications in cancer treatment

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  • Preparation method of bispecific antibody NK cells, and cells and application thereof
  • Preparation method of bispecific antibody NK cells, and cells and application thereof

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preparation example Construction

[0047] The present application provides a method for preparing bispecific antibody NK cells, comprising the following steps:

[0048] S1: Culture NK cells;

[0049] S2: Construction of CD16A / HSP70 double anti-lentiviral expression vector;

[0050] S3: Infect the NK cells with the CD16A / HSP70 double-antibody lentiviral expression vector to obtain CD16A / HSP70 antibody bispecific antibody NK cells.

[0051] Compared with T cells and B cells, the specificity of NK cell surface markers is relative. Human NK cells mIg-, some and NK cells were positive for CD2, CD3 and CD8, expressed IL-2 receptor β chain (P75, CD122), and were positive for CD11b / CD18. Commonly used marker antibodies for detecting NK cells include CD16, CD56, CD57, CD59, CD11b, CD94 and LAK-1.

[0052] The present application provides bispecific antibody NK cells prepared according to the above method.

[0053] The present application also provides the application of the bispecific antibody NK cells prepared acco...

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Abstract

The invention provides a preparation method of bispecific antibody NK cells, and cells and application thereof. The method comprises the following steps of: culturing NK cells, constructing a CD16A / HSP70 bispecific antibody lentiviral expression vector, and infecting the NK cells by the CD16A / HSP70 bispecific antibody lentiviral expression vector so as to obtain the bispecific antibody NK cells ofa CD16A / HSP70 antibody. The NK cells prepared by the invention are excellent in cell-specific phenotype and high in purity, wherein CD3-CD56+ achieves 97.13%; CD3-CD56+CD16+ achieves 93%; and the NKcells carrying the CD16A / HSP70 bispecific antibody lentiviral expression vector can effectively eliminate liver cancer cells and inhibit increasing of the tumour volume of the liver cancer.

Description

technical field [0001] The present application relates to the field of cell culture technology, in particular to a method for preparing bispecific antibody NK cells, cells and applications thereof. Background technique [0002] Natural killer cells (NK) are important immune cells in the body, not only related to anti-tumor, anti-viral infection and immune regulation, but also involved in the occurrence of hypersensitivity and autoimmune diseases in some cases, and can recognize Target cells, killing mediators. Natural killer cells are derived from bone marrow lymphoid stem cells. Their differentiation and development depend on the microenvironment of bone marrow and thymus, and are mainly distributed in bone marrow, peripheral blood, liver, spleen, lung and lymph nodes. NK cells are different from T and B cells, and are a type of lymphocytes that can non-specifically kill tumor cells and virus-infected cells without prior sensitization. [0003] The source of NK is mainly ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/867C12N15/13C12N5/10A61K39/395A61P35/00A61P1/16
CPCC12N15/86C07K16/283C07K16/18C12N5/0646A61P35/00A61P1/16C12N2740/15043C12N2800/107C07K2317/31C12N2510/00A61K2039/507A61K2039/5156A61K2039/844
Inventor 张扬周晓宇李陶张立忠
Owner 中科宝承生物医学科技有限公司
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