Duck novel micro ribonucleic acid virus loop-mediated isothermal amplification detection primer group and kit
A microRNA, ring-mediated isothermal technology, applied in the field of poultry disease detection, to achieve the effect of simple determination method, fast operation, and easy operation
Pending Publication Date: 2021-03-09
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology
[0004] At present, there are no relevant research reports on the loop-mediated isothermal amplification (LAMP) reaction method for duck novel microRNA virus (DuMV), and the establishment of the present invention can fill in the gaps in related fields at home and abroad
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Embodiment 1
[0026] 1. Experimental method
[0027] 1 Test strains and bacterial strains
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Abstract
The invention relates to a duck novel micro ribonucleic acid virus loop-mediated isothermal amplification detection primer group and a kit. According to the method, a primer group is designed according to a duck novel micro ribonucleic acid virus genome conserved sequence, wherein the primer group comprises outer primers (DUMV-F3 and DUMV-B3), inner primers (DUMV-FIP and DUMV-BIP) and a loop primer (DUMV-LB). The specific sequences are as shown in SEQ ID NO.1-5. The duck novel micro ribonucleic acid virus loop-mediated isothermal amplification detection primer group and the kit are establishedaccording to the designed primer group. A detection method of the kit has no cross reaction with pathogens of common infectious diseases of ducks. The detection method disclosed by the invention is strong in specificity, good in accuracy, high in sensitivity, free of expensive instruments and equipment, convenient for basic clinical field use, convenient and rapid.
Description
technical field [0001] The invention belongs to the field of poultry disease detection, and in particular relates to a duck novel microRNA virus loop-mediated isothermal amplification detection primer set and kit. Background technique [0002] Loop-mediated isothermal amplification technology (loop-mediated isothermal amplification, LAMP) is a constant temperature amplification technology for target genes in a water bath by using 4-6 specific primers through BstDNA polymerase. With strong amplification specificity, high sensitivity, quick and easy operation, and simple detection, it gets rid of the dependence on expensive instruments such as PCR machines and real-time fluorescent quantitative PCR machines, and makes detection in grassroots units more convenient. Compared with other existing nucleic acid amplification technologies, LAMP has many unique advantages: ①The specificity of LAMP technology amplification is very high, the specific primers used can identify specific r...
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IPC IPC(8): C12Q1/70C12Q1/6844C12N15/11
CPCC12Q1/701C12Q1/6844C12Q2531/119C12Q2537/1376C12Q2563/107
Inventor 陈翠腾万春和黄瑜陈珍朱春华刘斌琼
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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