Method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea hongkongensis triploid
A Portuguese oyster and diploid technology, which is applied in the hybrid production of Portuguese oyster and Hong Kong giant oyster to produce oyster allotetraploid, can solve the problems of low survival rate, poor taste and can only be used for barbecue, etc., and has broad prospects for popularization and application Effect
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[0035] (a) Selection and maturation promotion of seed oysters: select 100 three-year-old Hong Kong giant oyster triploids (shell height 10-15 cm) with large individuals, good vitality, and no damage as reproductive females, from 1000 second-year-old Portuguese oysters The diploid (shell height 8-12cm) intercepts the individual with the top 10% of the total weight as the breeding male parent. Then put the seed shells into the seed shell cultivation ponds and adopt the flowing water method to promote ripening. The biological bait is mainly artificially cultivated golden algae, chaetoceros and flat algae, and the algae water in the earthen pond is used as a backup bait source.
[0036] (b) Acquisition of triploid eggs: After the gonads of Hong Kong giant oyster species have matured, 30 triploid individuals of Hong Kong giant oyster were detected by a ploidy analyzer, and a total of 24 3N individuals were detected, which were screened out through microscope observation Ten female ...
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