Method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea hongkongensis triploid

A Portuguese oyster and diploid technology, which is applied in the hybrid production of Portuguese oyster and Hong Kong giant oyster to produce oyster allotetraploid, can solve the problems of low survival rate, poor taste and can only be used for barbecue, etc., and has broad prospects for popularization and application Effect

Inactive Publication Date: 2021-03-23
世倍(厦门)海洋科技有限公司
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AI Technical Summary

Problems solved by technology

However, the breeding cycle of giant oysters in Hong Kong is too long, and it usually takes 2 to 3 years to be marketed. The survival rate is low. Due to the long-term living in the estuary area, the taste is poor and it can only be used for barbecue, etc.

Method used

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  • Method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea hongkongensis triploid
  • Method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea hongkongensis triploid

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no. 2 example

[0035] (a) Selection and maturation promotion of seed oysters: select 100 three-year-old Hong Kong giant oyster triploids (shell height 10-15 cm) with large individuals, good vitality, and no damage as reproductive females, from 1000 second-year-old Portuguese oysters The diploid (shell height 8-12cm) intercepts the individual with the top 10% of the total weight as the breeding male parent. Then put the seed shells into the seed shell cultivation ponds and adopt the flowing water method to promote ripening. The biological bait is mainly artificially cultivated golden algae, chaetoceros and flat algae, and the algae water in the earthen pond is used as a backup bait source.

[0036] (b) Acquisition of triploid eggs: After the gonads of Hong Kong giant oyster species have matured, 30 triploid individuals of Hong Kong giant oyster were detected by a ploidy analyzer, and a total of 24 3N individuals were detected, which were screened out through microscope observation Ten female ...

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Abstract

The invention discloses a method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea hongkongensis triploid. According to the method, the crassostrea hongkongensistriploid is used as a female parent, the full weight is used as a target, the crassostrea angulata diploid is preferably selected as a male parent, and the oyster allotetraploid is produced through drug-induced hybridization. The oyster allotetraploid obtained by the method has the characteristics of euryhalinity and high temperature resistance, is suitable for breeding in low-salt areas such asestuary, and the oyster triploid produced by hybridizing the oyster allotetraploid with the common crassostrea angulata or crassostrea hongkongensis diploid is suitable for aquaculture in sea areas with different salt contents in China , and has wide popularization and application prospects.

Description

technical field [0001] The invention relates to a method for producing allotetraploid by shellfish hybridization, in particular to a method for hybrid production of oyster allotetraploid by Portuguese oyster and Hong Kong giant oyster. Background technique [0002] As an important marine organism, oyster has delicious meat and high nutritional value, and is called "milk in the sea". Oysters have a wide geographical distribution, fast growth, high yield, and high economic value. They are important mariculture objects in all countries in the world. [0003] The growth advantage of triploid oyster is obvious. Triploid oysters have 3 sets of chromosomes, so fecundity is extremely low, which means that on the one hand they can grow faster (their energy is used to grow rather than reproduce), and on the other hand they do not produce Or rarely produce gametes, which can ensure stable quality. Triploid oysters are formed by crossing tetraploid oysters with diploid oysters, so it...

Claims

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Application Information

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IPC IPC(8): A01K61/54A01K61/17
CPCA01K61/17A01K61/54Y02A40/81
Inventor 朱李贤
Owner 世倍(厦门)海洋科技有限公司
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