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A high-efficiency method for inducing differentiation of human mesenchymal stem cells into lipids

A technique for inducing differentiation of mesenchymal stem cells, which is applied in the field of high-efficiency human mesenchymal stem cells to induce differentiation into lipids, to achieve the effect of improving efficiency and reducing damage

Active Publication Date: 2021-12-21
华夏源(上海)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the advancement of science and technology, researchers are committed to the development of mesenchymal stem cells, mainly because of their more clinical applications, and they need to play a greater role in overcoming some intractable diseases that humans are currently facing.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat, comprising the following steps:

[0050] S01: cell culture: human mesenchymal stem cells were added into MM3 medium and cultured for 1 day.

[0051] S02: Inducing cell differentiation: adding a lipid-inducing reagent to the medium to induce differentiation of mesenchymal stem cells, and changing the lipid-inducing reagent every 48 hours; inducing differentiation for 7 days;

[0052] S03: Judging the degree of cell differentiation: After the lipid droplets appeared in the differentiated cells, Sudan Red 5B was added to identify the degree of cell differentiation.

[0053] MM3 medium is a medium composed of serum replacement and mesenchymal stem cell basal medium; the volume ratio of serum replacement and mesenchymal stem cell basal medium is 1:19.

[0054]Sudan Red 5B, purchased from Israel Biological Industries (BioInd) company; mesenchymal stem cell basal medium, model 05-2...

Embodiment 2

[0056] A high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat, the specific implementation of which is the same as in Example 1, except that the volume ratio of serum replacement and mesenchymal stem cell basal medium is 1:15 .

Embodiment 3

[0058] A high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat, its specific implementation is the same as in Example 1, the difference from Example 1 is that the time for inducing differentiation in step S02 is 10 days.

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Abstract

The invention belongs to the field of biotechnology, and more specifically relates to a high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat. A high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat, comprising the following steps: S01: culturing cells; S02: inducing cell differentiation; S03: judging the degree of cell differentiation. The method for inducing differentiation of human mesenchymal stem cells into lipids developed by the present invention can be differentiated efficiently and quickly, avoiding the phenomenon of reduction in the process of cell differentiation; the method developed by the present invention can ensure that there will be a large area of ​​lipids within 7 days of differentiation. The emergence of droplets ensures the smooth progress of cell differentiation; the method developed by this application improves the efficiency of cell culture differentiation, reduces the influence of carbon dioxide and other substances in the air on the medium and thus damages the cell culture process.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a high-efficiency method for inducing differentiation of human mesenchymal stem cells into fat. Background technique [0002] Mesenchymal stem cells are a kind of pluripotent stem cells, which have all the common characteristics of stem cells, that is, self-renewal and multidirectional differentiation capabilities. With the advancement of science and technology, researchers are committed to the development of mesenchymal stem cells, mainly because they are widely used in clinical practice, and they need to play a greater role in overcoming some intractable diseases that humans are currently facing. [0003] The research on the differentiation of mesenchymal stem cells into lipid is also an important research direction. Especially at the current stage with the development of the beauty repair industry, adipose tissue is the source of seed cells for rebuilding and repa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0653C12N2506/1369C12N2506/1384C12N2506/1392C12N2506/025
Inventor 朱灏
Owner 华夏源(上海)生物科技有限公司
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