A kind of culture solution for colorectal cancer organoid culture and preparation method thereof

A technology of colorectal cancer and culture fluid, which is applied in the field of biomedicine, can solve the problems of lack of research models, and the survival rate of patients with treatment efficiency has not been significantly improved, and achieve the effect of simple preparation method, easy operation, and low cost

Active Publication Date: 2022-07-15
杭州艾名医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The underlying mechanisms of colorectal cancer pathobiology are currently poorly understood, and treatment efficiency and patient survival have not significantly improved, mainly due to the lack of suitable research models

Method used

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  • A kind of culture solution for colorectal cancer organoid culture and preparation method thereof
  • A kind of culture solution for colorectal cancer organoid culture and preparation method thereof
  • A kind of culture solution for colorectal cancer organoid culture and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0070] The culture solution for colorectal cancer organoid culture in this example, each 50mL culture solution is made from the following raw materials: icabet sodium, with a final concentration of 1 μg / mL; vitamin P4, with a final concentration of 100 μM; human recombinant protein R-Spondin 1, the final concentration is 30ng / mL; Noggin, the final concentration is 100ng / mL; Human recombinant protein rhEGF, the final concentration is 8ng / mL; 4-Hydroxyethylpiperazineethanesulfonic acid, the final concentration is 1mM; L-alanyl-L-glutamine, the final concentration is 1×; green chain double antibody, the final concentration is 1×; N2 additive, the final concentration is 1×; B27 additive, the final concentration is 1×; N-acetyl- L-cysteine, 0.09 mM final; Alk inhibitor A83-01, 0.5 μM final; 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4 -pyridyl)-1H-imidazole at a final concentration of 0.3 μM; gastrin at a final concentration of 10 nM; non-essential amino acid solution at a final con...

Embodiment 2

[0074] The culture solution for colorectal cancer organoid culture in this example, each 50mL culture solution is made from the following raw materials: icabet sodium, the final concentration is 50 μg / mL; vitamin P4, the final concentration is 50 μM; human recombinant protein R-Spondin 1, the final concentration is 40ng / mL; Noggin, the final concentration is 90ng / mL; Human recombinant protein rhEGF, the final concentration is 9ng / mL; 4-Hydroxyethylpiperazineethanesulfonic acid, the final concentration is 1mM; L-alanyl-L-glutamine, the final concentration is 1×; green chain double antibody, the final concentration is 1×; N2 additive, the final concentration is 1×; B27 additive, the final concentration is 1×; N-acetyl- L-cysteine, 0.11 mM final; Alk inhibitor A83-01, 0.4 μM final; 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4 -pyridyl)-1H-imidazole at a final concentration of 0.4 μM; gastrin at a final concentration of 10 nM; non-essential amino acid solution at a final concentrat...

Embodiment 3

[0078] The culture solution for colorectal cancer organoid culture in this example, each 50mL culture solution is made from the following raw materials: icabet sodium, with a final concentration of 100 μg / mL; vitamin P4, with a final concentration of 1 μM; human recombinant protein R-Spondin 1, the final concentration is 50ng / mL; Noggin, the final concentration is 80ng / mL; Human recombinant protein rhEGF, the final concentration is 10ng / mL; 4-Hydroxyethylpiperazineethanesulfonic acid, the final concentration is 1mM; L-alanyl-L-glutamine, the final concentration is 1×; green chain double antibody, the final concentration is 1×; N2 additive, the final concentration is 1×; B27 additive, the final concentration is 1×; N-acetyl- L-cysteine ​​at 0.125 mM final; Alk inhibitor A83-01 at 0.3 μM final; 4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4 -Pyridyl)-1H-imidazole at a final concentration of 0.5 μM; gastrin at a final concentration of 10 nM; non-essential amino acid solution at a fi...

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Abstract

The invention discloses a culture solution for culturing colorectal cancer organoids and a preparation method thereof, belonging to the technical field of biomedicine. The culture solution is made from the following raw materials: icabet sodium, vitamin P4, human recombinant protein R-Spond in 1, noggin, human recombinant protein rhEGF, 4-hydroxyethylpiperazine ethanesulfonic acid, L-alanine Acyl-L-Glutamine, Green Chain Double Antibody, N2 Additive, B27 Additive, N-Acetyl-L-Cysteine, Al k Inhibitor A83-01, 4-(4-Fluorophenyl)-2- (4-Hydroxyphenyl)-5-(4-pyridyl)-1H-imidazole, gastrin, non-essential amino acid solution, nicotinamide, and Advanced DMEM / F-12 medium. The culture medium of the invention can enable the colorectal cancer tissue cells to maintain a very high cell and tissue activity during the culture process, and better express their inherent pathological characteristics.

Description

technical field [0001] The invention relates to a culture solution for culturing colorectal cancer organoids and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Colorectal cancer is one of the most common malignant tumors that seriously threaten human health. The incidence of colorectal cancer in my country ranks third among all malignant tumors. The mortality rate of colorectal cancer ranks fifth among all malignant tumors. With the development of the economy, the improvement of living standards and the change of lifestyle, the incidence of colorectal cancer will continue to rise. [0003] Colorectal cancer is a complex disease. The occurrence and development of colorectal cancer is a dynamic process, involving the interaction of many signal molecules, forming a complex molecular regulatory network. At the same time, it is also affected by external environmental factors. The etiology of colorectal cancer is The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/38C12N2501/11C12N2500/30C12N2500/33C12N2501/998C12N2501/999
Inventor 游明亮王磊申重阳
Owner 杭州艾名医学科技有限公司
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