In-process device and method for cell culture monitoring
A monitoring device and cell culture technology, applied in biochemical equipment and methods, character and pattern recognition, capturing objects visible under a microscope, etc., can solve the problems of high cost of biological processing equipment
Pending Publication Date: 2021-07-30
GLOBAL LIFE SCI SOLUTIONS USA LLC
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- Application Information
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[0005] Furthermore, the present inventors have recognized that customers may not be willing to spend substantial sums on disposed of bioprocessing equipment, so it is necessary to reduce costs where possible, which means that low-cost monitoring devices are desirable, wherein the Higher cost features are reusable
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[0015] figure 1 A cell culture apparatus 10 is shown, which in this example comprises: a cell culture container 12, which in this embodiment is formed by a flexible bag; a cell culture mixture 14, which is mammalian cell (The mammalian cells have a tendency to aggregate together) and liquid cell culture media, including known cell growth media. The apparatus 10 further includes a rocking platform 16 that gently mixes the cell mixture 14 while rocking. The platform 16 is supported on a base 18 .
[0016] The cell culture mixture 14 can be monitored by a monitoring device 100 which in this example is attached to the exterior of the bag 12 and has an inlet and an outlet in fluid communication with the interior of the bag 12 and thus with the cell culture mixture 14 ( described in more detail below). The device 100 further comprises a temporary umbilical 102 which in this embodiment comprises electrical power supply and signal lines (electrical and / or optical fiber).
[0017] ...
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Disclosed is an in-process cell monitoring device comprising: a flow channel having at least one inlet and at least one outlet exposeable to a cell culture; a microscope positionable to view the contents of a region of the channel; and a computer operable at least to count any cells in the region, providing a closed fluid circuit for cell monitoring. Also disclosed is a bioreactor. The bioreactor includes a cell culture volume and a cell monitoring device in the process, The device includes: a flow channel, wherein the flow channel has at least one inlet and at least one outlet each in fluid communication with the volume, and the flow channel has a sufficient cross-sectional area to allow fluid to be discharged from the inlet to the outlet; and a microscope, wherein the microscope can be positioned to view the contents of the area of the channel, and also disclosed is a method for monitoring cell culture, the method including determining the cell density.
Description
technical field [0001] The present invention relates to a device for in-process monitoring of cell density and cell viability by imaging of cells, eg in the field of immunotherapy cell culture. Background technique [0002] Important parameters of cell growth that generally mimic their in vivo state are now generally recognized, and for a particular cell line (eg, a cell line used to express a monoclonal antibody), its cell culture parameters can be predicted with considerable accuracy based on prior experience. Thus, even cell cultures with continuously fed batch cultures can be easily optimized. However, in cases where the characteristics of the seed cells of a cell culture batch are largely unknown or variable, for example in a single batch of autologous immunotherapy cell culture, then to some extent the cell culture parameters must be based on culture sampling during the culture process. to adjust in order to optimize that process. [0003] Various sensors are commerc...
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IPC IPC(8): G06K9/00
CPCG06V20/693G06V20/698G06V20/66C12M27/16C12M41/44C12M41/46G06T7/0012G06T7/20G06T2207/10056G06T2207/30024G06T2207/30242
Inventor S·普拉尼克M·K·拉玛克里希纳H·帕蒂尔
Owner GLOBAL LIFE SCI SOLUTIONS USA LLC