Biological membrane capable of inducing bone regeneration as well as preparation method and application of biological membrane
A biofilm and bone regeneration technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of incomplete removal of immunogenic substances and destruction of biological tissue structure, achieving small damage to components and three-dimensional structure, and strong repair ability , the effect of retaining biological activity
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[0034] The preparation method comprises: taking fresh peritoneal tissue of mammals, freezing and thawing repeatedly to break the cells, removing part of the immunogenic substance, and using sodium bicarbonate powder to remove the fat and excess serosa on the fat surface of the peritoneum to obtain a uniform rough structure. Repeatedly use divalent metal ion chelating agent, hypertonic alkali solution, hypotonic alkali solution, and hypertonic acid solution to remove immunogenic substances in order to remove lipids, cell structures, and various DNA and RNA components in peritoneal tissue. And after cleaning, it can be sterilized by irradiation. In the process of preparing biofilm, the present invention does not use surfactants, avoids the difficulty of removing surfactants, and does not introduce cross-linking agents and synthetic materials, so it will not have potential cytotoxicity, and will not cause fibrosis, For reactions such as chronic inflammation, the reaction conditi...
Embodiment 1
[0063] Preparation of a calf peritoneal biofilm that can induce bone regeneration:
[0064] Take fresh calf peritoneum tissue, place it at -20°C, freeze and thaw it repeatedly for 3 times, take it out, flatten the peritoneum and place it on an ice pack with the fat side facing up, add 0.5 grams per square centimeter to the fat side Sprinkle sodium bicarbonate powder on the side, scrub to remove fat and excess serosa, and rinse with running water for 12 hours. The above is the pretreatment step. Transfer the membrane into 0.5mol / L EDTA solution, shake for 15h at 25°C, then transfer to 3% NaOH solution, shake at room temperature for 2h, transfer the membrane into 0.5mol / L EDTA solution, 25 Under the condition of ℃, after shaking for 2 hours, transfer to 3% NaOH solution, shake at room temperature for 2 hours, transfer the film to 0.1% NaOH solution and shake, and change NaOH solution 3 times during each shaking for 30 minutes. The treatment after the above pretreatment steps wa...
Embodiment 2
[0066] Preparation of a porcine peritoneal biofilm that can induce bone regeneration:
[0067] Take fresh porcine peritoneum tissue, place it at -20°C, freeze and thaw it repeatedly for 3 times, take it out, flatten the peritoneum on an ice pack, with the fat side facing up, and add 0.5 grams per square centimeter to the side of the fat side Sprinkle with sodium bicarbonate powder, scrub with your hands to remove fat and excess serosa, and rinse with running water for 12 hours. The above is the pretreatment step. Transfer the membrane into 0.5mol / L EDTA solution, shake for 15 hours at 25°C, transfer to 2% NaOH solution, shake at room temperature for 2h, transfer the membrane into 0.5mol / L EDTA solution, 25 Under the condition of ℃, after shaking for 2 hours, transfer to 2% NaOH solution, shake at room temperature for 2 hours, transfer the membrane to 0.5% NaOH solution and shake, and change the NaOH solution 3 times during each shaking for 30 minutes. The treatment after the ...
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