Biological membrane capable of inducing bone regeneration and preparation method of biological membrane
A biofilm and bone regeneration technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of incomplete removal of immunogenic substances and destruction of biological tissue structure, achieving small damage to components and three-dimensional structure, and strong repair ability , The preparation method is simple and effective
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034]Take fresh calf peritoneum tissue, place it at -20°C, freeze and thaw it repeatedly for 3 times, take it out, flatten the peritoneum and place it on an ice pack with the fat side facing up, add 0.5 grams per square centimeter to the fat side Sprinkle sodium bicarbonate powder on the side, scrub to remove fat and excess serosa, and rinse with running water for 12 hours. The above is the pretreatment step. Transfer the membrane into 0.5mol / L EDTA solution, shake at 25°C for 15h, then transfer to 3% NaOH solution, shake at room temperature for 2h, transfer the membrane into 0.5mol / L EDTA solution, 25 Under the condition of ℃, after shaking for 2 hours, transfer to 3% NaOH solution, shake at room temperature for 2 hours, transfer the membrane to 0.1% NaOH solution and shake, and change the NaOH solution 3 times during each shaking for 30 minutes. The treatment after the above pretreatment steps was repeated 3 times. Put the above treated membrane into 0.5%HCl-1%NaCl solutio...
Embodiment 2
[0036] Take fresh porcine peritoneum tissue, place it at -20°C, freeze and thaw it repeatedly for 3 times, take it out, flatten the peritoneum on an ice pack, with the fat side facing up, and add 0.5 grams per square centimeter to the side of the fat side Sprinkle with sodium bicarbonate powder, scrub with your hands to remove fat and excess serosa, and rinse with running water for 12 hours. The above is the pretreatment step. Transfer the membrane into 0.5mol / L EDTA solution, shake at 25°C for 15h, transfer to 2% NaOH solution, shake at room temperature for 2h, transfer the membrane into 0.5mol / L EDTA solution, 25 Under the condition of ℃, after shaking for 2 hours, transfer to 2% NaOH solution, shake at room temperature for 2 hours, transfer the film to 0.5% NaOH solution and shake, and change the NaOH solution 3 times during the period, shaking for 30 minutes each time. The treatment after the above pretreatment steps was repeated 3 times. Put the above treated membrane in...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Conductivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More