A SNP Molecular Marker Related to Chicken Carcass Traits and Its Application
A technology for molecular markers and carcass traits, which can be used in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., and can solve problems such as few specific effects
Active Publication Date: 2022-07-01
SOUTH CHINA AGRI UNIV
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Problems solved by technology
The specific role of this gene in birds has been rarely reported, especially in chickens
Method used
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Embodiment 1
[0033] 1. Animal materials
[0034] The same batch of 731 ephedra chickens (Fuhe Breeder Farm of Guangdong Jiangfeng Company) were raised under the same feeding conditions. Each chicken has a unique identification (foot number). At 100 days of age, 1.2 mL of subwing venous blood was collected using a vacuum anticoagulant tube, and stored in a -80°C low-temperature refrigerator for subsequent DNA extraction. 100-day-old chickens were slaughtered, divided and sampled, and the slaughter weight, half evisceration weight, full evisceration weight, shank length, body oblique length and other traits were determined.
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The invention discloses a SNP molecular marker related to chicken carcass traits and its application, and belongs to the technical field of gene detection. The SNP molecular marker corresponds to a point mutation at the NC_006088.5: 25921381 site in the chicken MDFIC gene, and the SNP mutation site 25921381: C>T, and there are three genotypes of CC, CT and TT. Experiments verified that the CC genotype individuals at this mutation site showed the highest carcass weight, half evisceration weight, full evisceration weight, the shortest shank length, and body oblique length, while the CT genotype individuals showed the lowest carcass weight, half evisceration weight, and half evisceration weight. The weight of the bore, the weight of the whole evisceration, the shortest shin length and the oblique length of the body. Therefore, the SNP molecular marker disclosed in the present invention helps to accurately and rapidly assist the early screening of meat production performance related to carcass traits, and accelerates the process of chicken genetics and breeding.
Description
technical field [0001] The invention relates to the technical field of gene detection, in particular to a SNP molecular marker related to chicken carcass traits and its application. Background technique [0002] Chicken is one of the most important agricultural livestock and poultry animals, and its meat production performance directly affects the market economic benefits. Compared with white-feathered broiler, yellow-feathered broiler has better meat quality and better flavor, but it also has greater weaknesses. Yellow-feathered broilers, especially local chicken breeds, generally have problems such as low feed conversion rate, low meat yield and poor reproductive performance, which have higher requirements on breeding costs and feed consumption. From the perspective of breeding, cultivating high-yield yellow-feather broilers can greatly drive the economic benefits of the yellow-feather broiler industry, facilitate the effective use of resources, and be of great significan...
Claims
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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 聂庆华黄育林张思雨郭利金温琪徐海平黎镇晖郑茗
Owner SOUTH CHINA AGRI UNIV