SNP (Single Nucleotide Polymorphism) molecular marker related to chicken carcass traits and application thereof
A technology for molecular markers and carcass traits, which can be used in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., and can solve problems such as few specific effects
Active Publication Date: 2022-01-11
SOUTH CHINA AGRI UNIV
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Problems solved by technology
The specific role of this gene in birds has been rarely reported, especially in chickens
Method used
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Embodiment 1
[0033] 1, animal material
[0034] The same batch of 731 Ma Huang Chicken (Guangdong Jiangfeng Company blessings and chicken farms), raised under the same feeding conditions. Each chicken has a unique identity (foot number). At 100 days of age, 1.2 ml of its end venous blood was collected using a vacuum anti-condensing pipe, and the cryopreservation of the -80 ° C was stored for subsequent DNA extraction. 100 days of age in slaughter, segmentation, and sampling, determine the body weight of the chicken, half net weight, all net weight, tabular, gangling length.
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The invention discloses an SNP molecular marker related to chicken carcass traits and application thereof, and belongs to the technical field of gene detection. The SNP molecular marker has point mutation corresponding to the NC_006088.5:25921381 site in the chicken MDFIC gene, the SNP mutation site 25921381:C is greater than T, and three genotypes of CC, CT and TT exist. The test verifies that the CC genotype individual at the mutation site shows the highest carcass weight, semi-eviscerating weight, full eviscerating weight, shortest shin length and oblique body length, and the CT genotype individual shows the lowest carcass weight, semi-eviscerating weight, full eviscerating weight, shortest shin length and oblique body length. Therefore, the SNP molecular marker disclosed by the invention is helpful for accurately and quickly assisting in early screening of meat production performance related to carcass traits and accelerating the progress of chicken genetic breeding.
Description
Technical field [0001] The present invention relates to the field of gene detection, and more particularly to a SNP molecule marker and application associated with chicken stroke. Background technique [0002] Chicken as one of the most important agricultural livestock and poultry animals, its performance directly affects market economic benefits. Compared to white meat chickens, the meat of Huang Yu meat chicken is better, the flavor is better, but there is also a big weakness. Huang Yu meat chicken, especially the local chicken variety, there is a low feed conversion rate, low productivity and poor reproductive performance, and has a greater demand for breeding costs and feed consumption. From the perspective of breeding, cultivation of high-yield meat, yellow and yellow chickens, can greatly bring the economic benefits of the yellow meat chicken industry, which is conducive to the effective use of resources, which is of great significance to promote the development of yellow f...
Claims
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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 聂庆华黄育林张思雨郭利金温琪徐海平黎镇晖郑茗
Owner SOUTH CHINA AGRI UNIV
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