Seeding method for chonemorpha crown spores

Abstract

The invention discloses a chonemorpha crown spore sowing method, and mainly relates to the technical field of plant seedling raising. Comprising the following steps: S1, collecting mature spores of the chonemorpha crown; s2, preparing a substrate for spore sowing; s3, spreading the spores in the matrix; s4, placing the substrate in which the spores are sown under the conditions that the humidity is 60%-85%, the temperature is 25-28 DEG C and the illumination is 3000LX-5000LX, observing the germination and growth conditions of the spores, and waiting for seedling formation; according to the method, complicated test procedures are greatly saved, manpower, consumables and cost are saved, and popularization of a breeding technology of germplasm resources of the Cynanchum otophyllum is facilitated.

Description

technical field [0001] 本发明涉及植物育苗技术领域,具体是一种皇冠鹿角蕨孢子播种方法。 Background technique [0002] 皇冠鹿角蕨(Platycerium coronarium)为鹿角蕨属中一种大型附生蕨类植物,营养叶厚而上扬,叶端分出深裂,叶面光滑,孢子叶分为长短两个分枝,叶基狭窄,叶端分出多层分枝,可达三米以上,是观赏性较高的附生蕨类植物,其孢子叶基部的叶裂上长出孢子囊堆,形如小勺,一般不分株繁殖,而是以孢子繁殖。 [0003] 但是,自然状态的孢子繁殖必须具有热带原始生境中才有可能,分株繁殖速度慢,系数低,难以得到大量的种苗;而对皇冠鹿角蕨的孢子进行组织培养时,需经过繁杂的操作和实验条件,周期较长,同时在培养基上的幼孢子体的成长速度慢,也有研究发现在皇冠鹿角蕨叶片和根部的离体培养中,培养瓶内容易产生乙烯,对皇冠鹿角蕨孢子体的生长有抑制作用,大大降低皇冠鹿角蕨成苗率。上述问题极大的限制了鹿角蕨种质资源的繁育技术推广。 Contents of the invention [0004] 本发明的目的在于解决现有技术中存在的问题,提供一种皇冠鹿角蕨孢子播种方法,极大的节省了繁杂的试验程序,节省人力、耗材和成本,有助于鹿角蕨种质资源的繁育技术推广。 [0005] 本发明为实现上述目的,通过以下技术方案实现: [0006] 一种皇冠鹿角蕨孢子播种方法,包括步骤: [0007] S1、采集皇冠鹿角蕨成熟孢子; [0008] S2、为孢子播种准备基质; [0009] S3、将孢子散播在基质中; [0010] S4、将播种有孢子的基质置于湿度60%-85%,温度25-28℃,光照3000LX-5000LX的条件下,观察孢子萌发长势情况,等待成苗。 [0011] 优选的,步骤S1中,采集成熟并开始散布的皇冠鹿角蕨孢子,置于叠放的200目、100目的不锈钢分样筛中,盖盖进行摇晃,过滤杂质、孢子囊,收集成熟孢子,并将孢子置于离心管中,盖好离心管的盖子后,放置于冰箱中备用。 [0012] 优选的,步骤S2中的基质包括草炭、树皮渣,将草炭与树皮渣过筛,以3:1的比例进行配比后装于带盖育苗盘中,装土至盘2 / 3处,1L基质加入2g长效缓释肥混合,用沸水浇透杀菌,...

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Problems solved by technology

[0003] However, the spore propagation in the natural state must be possible in the tropical primitive habitat, the ramet propagation speed is slow, the coefficient is low, and it is difficult to obtain a large number of seedlings; and when the spores of the crown staghorn fern are tissue cultured, it is necessary to go through complicated operations. And experimental conditions, the cycle is longer, and the growth rate of young sporophytes on the medium is slow, and some studies have also found that in the in vitro culture of staghorn fern leaves and roots, ethylene is easily produced in the culture bottle, which is harmful to the staghorn fern spores. The growth of staghorn fern can be inhibited, greatly reducing the seedling rate of staghorn fern

The above problems have greatly limited the promotion of the breeding technology of staghorn fern germplasm resources

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