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Method for establishing disinfection drug screening test model

A technology for screening tests and establishing methods, which is applied in the fields of drug screening, compound screening, biochemical equipment and methods, etc. It can solve problems affecting the survival rate, the survival rate of fish fry is less than 5%, and the effect of disinfection drugs. Simple, effective in controlling fish epidemics and reliable disinfection effect

Pending Publication Date: 2022-05-06
北京市水产技术推广站(北京市鱼病防治站)
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Problems solved by technology

[0002] Rainbow trout (including golden trout) eggs are most susceptible to virus (Infectious Hematopoietic Necrosis Virus, IHNV) infection during the artificial incubation process, which affects their survival rate, resulting in the survival rate of hatched fry due to virus infection. Usually less than 5 %
However, rainbow trout eggs are mainly hatched at a water temperature of 8-12 degrees. At this water temperature, the disinfection effect of many disinfectant drugs is significantly affected; and the current disinfectant drugs on the market are mixed, and some of them are counterfeit.
There is no effective test model in the prior art that can screen out reliable disinfectants suitable for low water temperatures

Method used

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  • Method for establishing disinfection drug screening test model

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Embodiment Construction

[0021] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0022] Such as figure 1 Shown, the establishment method of disinfection drug screening test model of the present invention comprises the following steps:

[0023] The first step, drug pre-cooling: put each disinfectant drug to be screened in a biochemical incubator, adjust the temperature to 10 degrees, and use it after 24 hours;

[0024] The second step, cell culture: insert two kinds of cells, such as EPC and FHM, into a 96-well plate, and culture at 25 degrees;

[0025] The third step, mixing: take IHNV, whose virus titer is 10 to the -6 power, mix with the pre-cooled disinfectant from different manufacturers, and place it at 10 degrees for 10-15min. Multiple concentration gradients can be set up for the disinfectant .

[0026] The fourth step, receiving cell inspection: dilute the mixed solution 10 times successively, from the o...

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Abstract

The invention discloses a method for establishing a disinfection drug screening test model, which comprises the following steps of: 1, precooling drugs: putting disinfection drugs to be screened into a biochemical incubator, adjusting the temperature to 8-12 DEG C, and using the disinfection drugs after 24 hours; 2, cell culture: inoculating EPC cells and FHM cells into a pore plate, and culturing at 25 DEG C; 3, mixing: taking the IHNV, and mixing the IHNV with the pre-cooled disinfection medicines from different manufacturers; 4, inoculating cells for inspection: diluting the mixed solution by 10 times in sequence, and inoculating the diluted mixed solution into a 96-well cell plate; simultaneously adding virus positive and negative controls; 5, passage inspection and drug determination, wherein the cells are continuously passed for several generations, and a disinfection drug which does not have lesions in the EPC cells and the FHM cells is used as a reliable disinfection drug.

Description

technical field [0001] The invention relates to the technical field of disinfection drug screening, in particular to a method for establishing a test model for disinfection drug screening of "infectious hematopoietic organ necrosis virus". Background technique [0002] Rainbow trout (including golden trout) eggs are most susceptible to virus (Infectious Hematopoietic Necrosis Virus, IHNV) infection during the artificial incubation process, which affects their survival rate, resulting in the survival rate of hatched fry due to virus infection. Usually less than 5 %. [0003] In order to ensure the successful hatching of eggs, the method of disinfecting eggs and facility tools is usually adopted, and disinfectants are generally used. However, rainbow trout eggs are mainly hatched at a water temperature of 8-12 degrees. At this water temperature, the disinfection effect of many disinfectant drugs is significantly affected; and currently, the disinfectant drugs on the market ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/077C12Q1/02
CPCC12N5/0692C12N5/0658G01N33/5064G01N33/5061C12N2503/02G01N2500/10
Inventor 徐立蒲
Owner 北京市水产技术推广站(北京市鱼病防治站)
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