Chromatographic device, chromatographic system and method for replacing chromatographic column

Abstract

The invention relates to a chromatographic device, a chromatographic system and a chromatographic column replacement method, the chromatographic device comprises a chromatographic box and a quick-insertion quick-change mechanism, the chromatographic box comprises a box body and a chromatographic column arranged in the box body, the two ends of the chromatographic column are connected with an adapter assembly penetrating through the side wall of the box body, the adapter assembly is provided with a first channel communicated with the chromatographic column, and the first channel is communicated with the chromatographic column. The quick-inserting and quick-changing mechanism comprises a support and a sample feeding and discharging unit arranged on the support, the support is provided with a positioning assembly used for positioning the chromatographic box, the sample feeding and discharging unit comprises two movable sample needles, the two sample needles are used for being connected with the two switching assemblies respectively, and when the chromatographic box is positioned on the support, the two switching assemblies are connected with the two movable sample needles. The sample needle can move between a first position and a second position; when the sample needle moves to the first position, the sample needle is inserted into the first channel of the switching assembly, so that the sample needle is communicated with the first channel; and when the sample needle moves to the second position, the sample needle is separated from the switching assembly. According to the invention, the chromatographic column can be rapidly replaced from the chromatographic device in a mode of replacing the chromatographic box.

Description

Technical Field

[0001] The present application relates to the technical field of analysis and purification equipment, and in particular to a chromatography device, a chromatography system and a chromatography column replacement method. Background Art

[0002] Chromatographic analysis is widely used in many fields such as chemistry, biochemistry, pharmacy, food science, environmental science, etc., for the analysis and purification of complex components in samples. For example, in drug development, it can be used to purify drugs; in environmental testing, it can be used to detect pollutants in water and air; in food science, it can be used to analyze food ingredients.

[0003] High performance liquid chromatography (HPLC) is an important branch of chromatographic analysis, and the chromatographic column is the key part of the HPLC system for separating the components in the sample. The chromatographic column is usually composed of a slender cylindrical container filled with a stationary phase (such as silica gel, polymer, etc.). When the mobile phase containing the sample (such as solvent, water, etc.) passes through the stationary phase, the stationary phase will interact with the components in the sample, thereby achieving the separation of the components in the sample. The performance of the chromatographic column directly affects the analysis results and repeatability, but as the chromatographic column is used for a longer time, the chromatographic column may have problems such as column blockage, chemical degradation, physical wear and matrix effect, which requires replacement of a new chromatographic column; and when the chromatographic analysis requires frequent sample switching, a new chromatographic column will also need to be replaced.

[0004] At present, there are mainly the following methods for replacing chromatographic columns: (1) Direct replacement method, that is, unscrew the joints at both ends of the fixed chromatographic column, remove the old chromatographic column from the HPLC system, install the new chromatographic column in the same position, and then retighten the joints at both ends of the fixed chromatographic column. The direct replacement method is not only inconvenient to operate, but also may cause the mobile phase pressure in the HPLC system to increase instantly during the replacement process, thereby causing impact on the components in the HPLC system (such as pumps, joints, etc.); in addition, air may be introduced during the replacement process, causing changes in the solvent strength and pH value of the mobile phase, thereby affecting the accuracy of the analysis results.

[0005] (2) Online replacement method, that is, through the online replacement interface of the HPLC system, the old chromatographic column is directly switched to the new chromatographic column while keeping the HPLC system running. The online replacement method is costly and complicated to operate, requiring professional personnel to operate. In addition, during the process of online replacement of chromatographic columns, the switching between the old and new chromatographic columns may cause temporary fluctuations in the analysis results, thereby affecting the accuracy of the analysis results.

[0006] (3) Chromatographic column regeneration method, that is, using specific chemical reagents or physical methods to regenerate blocked or degraded chromatographic columns to restore their performance. The regeneration process of chromatographic columns is relatively complicated and requires professional chemical knowledge and equipment; moreover, the performance of the regenerated chromatographic column cannot be completely restored to its initial state, which affects the long-term stability and accuracy of the analysis results.

[0007] (4) Spare column method, that is, a spare column is installed in the HPLC system. When the main column needs to be replaced, it only needs to be switched to the spare column. This method requires the use of an HPLC system with a spare column and the ability to automatically switch columns. The spare column needs to be regularly maintained and calibrated, which increases the complexity and cost of the HPLC system. In addition, if the spare column is not used for a long time, its performance may deteriorate, thereby affecting the accuracy of the analysis results. Summary of the invention

[0008] Based on this, it is necessary to provide a chromatographic device, a chromatographic system and a method for replacing a chromatographic column that can simply and quickly replace a chromatographic column.

[0009] A chromatographic device comprises a chromatographic box and a quick-insert and quick-change mechanism, wherein the chromatographic box comprises a box body and a chromatographic column arranged in the box body, both ends of the chromatographic column are connected with adapter assemblies, two of the adapter assemblies are both penetrated through the side wall of the box body, the adapter assembly is provided with a first channel, the first channel is communicated with the interior of the chromatographic column, the quick-insert and quick-change mechanism comprises a bracket and a sample inlet and outlet unit arranged on the bracket, the bracket is provided with a positioning assembly, the positioning assembly is used to position the chromatographic box, the sample inlet and outlet unit comprises two movable sample needles, the two sample needles are used to be connected with two adapter assemblies respectively, when the chromatographic box is positioned on the bracket by the positioning assembly, the sample needle can move between a first position and a second position, when the sample needle moves to the first position, the sample needle is inserted into the first channel of the adapter assembly to communicate the interior of the sample needle with the first channel, when the sample needle moves to the second position, the sample needle and the adapter assembly are separated.

[0010] In one embodiment, the quick-insert and quick-change mechanism further includes a clamping assembly, which includes a clamping member, a connecting member, and a first elastic member. One end of the connecting member is rotatably connected to the bracket, and the other end of the connecting member is connected to the pressing member. The first elastic member acts on the connecting member or the pressing member to press the pressing member against the chromatographic box.

[0011] In one embodiment, two ends of the adapter assembly are respectively disposed on two sides of the side wall of the box body; The adapter assembly includes a sealing member, a sealing mounting seat and a sealing mounting cover, wherein the sealing mounting seat is disposed through the side wall of the box body, the sealing mounting seat and the sealing mounting cover are detachably connected, and the sealing mounting seat and the sealing mounting cover enclose a sealed space, the sealing member is disposed in the sealed space, and the sealing member is provided with the first channel; When the sample needle is in the first position, the sample needle is inserted into the sealing mounting cover, and the outer wall of the sample needle is tightly matched with the first channel.

[0012] In one of the embodiments, the adapter assembly further includes a sealing pressure ring, which is disposed between the sealing member and the sealing mounting cover to press the sealing member tightly onto the sealing mounting seat.

[0013] In one embodiment, a heating sub-box is provided in the chromatography box, and a tightly fitting pressure plate and a heating component are provided in the heating sub-box, a fixing groove is provided between the pressure plate and the heating component, the fixing groove is used to accommodate at least part of the chromatography column, and the heating component is used to heat the chromatography column accommodated in the fixing groove.

[0014] In one embodiment, the chromatographic column is connected to the adapter assembly via a pipeline, the pipeline includes an injection pipeline, one end of the injection pipeline is connected to the first channel of one of the adapter assemblies, and the other end of the injection pipeline is connected to the injection port of the chromatographic column, the fixed groove is also used to accommodate part of the injection pipeline, and the heating assembly is also used to heat the part of the injection pipeline accommodated in the fixed groove.

[0015] In one embodiment, the heating assembly includes a heating element, a heat conducting element, a temperature sensor and a temperature protection switch, one side of the heat conducting element is connected to the heating element, the other side of the heat conducting element is tightly matched with the pressing plate, and the fixing groove is surrounded between the pressing plate and the heat conducting element. The heating element is used to heat the chromatographic column accommodated in the fixing groove; The temperature sensor is used to detect the temperature of the heat conducting member; The temperature sensor and the heating element are both in communication connection with the temperature protection switch, and the temperature protection switch is used to turn on or off the heating element according to the temperature detected by the temperature sensor.

[0016] In one embodiment, a socket is further provided in the box body, a smart chip is provided in the socket, and the socket is passed through the box body; The bracket is provided with a plug-in component, and when the chromatographic box is positioned on the bracket by the positioning component, the plug-in component is plugged into the socket.

[0017] The present application also provides a chromatography system, comprising the chromatography device as described in any of the above embodiments.

[0018] The present application also provides a method for replacing a chromatographic column, the replacement method being used for replacing a chromatographic column in a chromatographic device in any of the above embodiments, the chromatographic box comprising a first chromatographic box and a second chromatographic box, the first chromatographic box being a chromatographic box positioned on the support of the chromatographic device before the chromatographic column is replaced, the second chromatographic box being a chromatographic box positioned on the support of the chromatographic device after the chromatographic column is replaced, The replacement method includes the following contents: The sample needle moves from the first position to the second position, and the sample needle is separated from the adapter component of the first chromatographic box; taking the first chromatography box out of the support; positioning the second chromatography cartridge on the support; The sample needle moves from the second position to the first position, the sample needle is inserted into the first channel of the adapter assembly of the second chromatographic box, and the sample needle is communicated with the first channel of the second chromatographic box, thereby completing the replacement of the chromatographic column.

[0019] Compared with the prior art, the chromatographic device provided by the present application includes a chromatographic box and a quick-insert quick-change mechanism, and the quick-insert quick-change mechanism includes a bracket and a sample inlet and outlet unit arranged on the bracket. The present application integrates the chromatographic column into the box body, and arranges the adapter assembly connected at both ends of the chromatographic column on the box body. When the chromatographic column needs to be replaced, only the entire chromatographic box needs to be replaced. Since a positioning member is arranged on the bracket, the chromatographic box is positioned by using the positioning member, and then by moving the sample needle, when the sample needle is moved to the first position, the inside of the sample needle is connected to the first channel. Since the first channel is connected to the inside of the chromatographic column, the inside of the sample needle and the inside of the chromatographic column can be connected, and the installation of the chromatographic box is realized; when the sample needle is moved to the second position, the sample needle and the adapter assembly are separated, so that the chromatographic box can be removed from the bracket, and the rapid disassembly of the chromatographic box is realized. It can be seen that the present application integrates the chromatographic column and the box body into a whole, and arranges a movable sample needle on the bracket, so that the rapid replacement of the chromatographic box can be realized. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the conventional technology, the drawings required for use in the embodiments or the conventional technology descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1A three-dimensional diagram of a chromatography device according to an embodiment of the present application; Figure 2 A schematic diagram of the internal structure of a chromatography box according to an embodiment of the present application; Figure 3 A cross-sectional view of a chromatography box according to an embodiment of the present application; Figure 4 for Figure 3 A partial enlarged view of the middle A; Figure 5 A schematic diagram of a quick-insert and quick-change mechanism according to an embodiment of the present application; Figure 6 This is a schematic diagram of a quick-insert and quick-change mechanism from another perspective of an embodiment of the present application.

[0022] Reference numerals: 10. Chromatographic box; 110. Box body; 111. Guide groove; 112. Limiting member; 113. Plug socket; 114. Handle; 120. Chromatographic column; 121. Injection pipe; 130, adapter assembly; 131, first channel; 132, seal; 133, seal mounting seat; 134, seal mounting cover; 135, seal pressure ring; 136, first avoidance hole; 137, second avoidance hole; 138, third avoidance hole; 140, heating sub-box; 141, bottom plate; 142, pressing plate; 143, heating assembly; 1431, fixing groove; 1432, heating element; 1433, heat conducting element; 1434, temperature sensor; 1435, temperature protection switch; 144, cover body; 20. Quick-insert and quick-change mechanism; 210, bracket; 211, first position detection member; 212, connector; 220, sample inlet and outlet unit; 221, sample needle; 222, driving assembly; 2221, fixing plate; 2222, pressing plate; 2223, second elastic member; 2224, guide shaft; 2225, stopper; 2226, groove; 223, pushing assembly; 2231, driving member; 2232, lead screw; 2233, slider; 2234, first guide rail; 2235, transmission group; 224, mounting seat; 225, second position detection member; 230, positioning assembly; 231, positioning groove; 232, first opening; 233, second opening; 234, positioning plate; 2341, first flange; 2342, second flange; 2343, third flange; 235, first plate; 236, guide rail; 240, clamping assembly; 241, clamping member; 242, connecting member; 243, first elastic member; 244, shock-absorbing pad. DETAILED DESCRIPTION

[0023] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0024] It should be noted that when a component is referred to as being "fixed to" or "disposed on" another component, it may be directly on the other component or there may be a central component. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be a central component at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in the specification of this application are for illustrative purposes only and do not represent the only implementation method.

[0025] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of this application, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0026] In the present application, unless otherwise clearly specified and limited, a first feature being “above” or “below” a second feature may mean that the first feature is directly in contact with the second feature, or the first feature and the second feature are indirectly in contact through an intermediate medium. Moreover, a first feature being “above”, “above”, or “above” a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being “below”, “below”, or “below” a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0027] Unless otherwise defined, all technical and scientific terms used in the specification of this application have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used in the specification of this application includes any and all combinations of one or more related listed items.

[0028] See also Figures 1 to 6The present application provides a chromatography device, including a chromatography box 10 and a quick-insert quick-change mechanism 20, the chromatography box 10 includes a box body 110 and a chromatography column 120 arranged in the box body 110, both ends of the chromatography column 120 are connected to adapter components 130, the two adapter components 130 are both penetrated through the side wall of the box body 110, the adapter component 130 is provided with a first channel 131, the first channel 131 is connected to the inside of the chromatography column 120, the quick-insert quick-change mechanism 20 includes a bracket 210 and a sample inlet and outlet unit 220 arranged on the bracket 210, the bracket 210 is provided with a positioning component 230, and the positioning component 230 is used to position The chromatographic box 10 and the sample inlet and outlet unit 220 include two movable sample needles 221, and the two sample needles 221 are used to be connected to the two adapter components 130 respectively. When the chromatographic box 10 is positioned on the bracket 210 by the positioning component 230, the sample needle 221 can move between a first position and a second position. When the sample needle 221 moves to the first position, the sample needle 221 is inserted into the first channel 131 of the adapter component 130 so that the interior of the sample needle 221 is connected to the first channel 131. When the sample needle 221 moves to the second position, the sample needle 221 and the adapter component 130 are separated.

[0029] In this embodiment, the adapter assembly 130 for connecting the chromatographic column 120 and the sample needle 221 of the quick-insert and quick-change mechanism 20 is inserted on the side wall of the box body 110 of the chromatographic box 10, and the chromatographic column 120 is integrated into the box body 110 of the chromatographic box 10. When the chromatographic column 120 needs to be replaced in the chromatographic device, the chromatographic box 10 with the old chromatographic column is directly removed, and another chromatographic box 10 with a new chromatographic column is installed to complete the replacement of the chromatographic column 120. That is, the present application integrates the chromatographic column 120 and the box body 110 into a whole, and sets a sample needle 221 that can move between a first position and a second position on the bracket 210, so that the sample needle 221 is connected or disconnected with the chromatographic column 120 inside the chromatographic box 10 through the first channel 131 of the adapter assembly 130, and the adapter assembly 130 is inserted on the side wall of the box body 110 of the chromatographic box 10. In this embodiment, the chromatographic column 120 can be quickly replaced by replacing the chromatographic box 10.

[0030] It is understandable that before installing the chromatographic box 10 with a new chromatographic column in the chromatographic device, the new chromatographic column needs to be installed in the chromatographic box 10 in advance, and the new chromatographic column needs to be connected to the adapter assembly 130. Because when preparing the chromatographic box 10 with the new chromatographic column, it is not necessary to suspend the operation of the chromatographic device, and it is sufficient to prepare it before removing the chromatographic box 10 with the old chromatographic column. For the chromatographic device, when the sample needle 221 is moved to the second position, the sample needle 221 and the adapter assembly 130 are separated, and the chromatographic box 10 with the old chromatographic column is directly taken out, and then the chromatographic box 10 with the new chromatographic column is placed at the target position. When the sample needle 221 is moved to the first position, the interior of the sample needle 221 is connected to the first channel 131. In this way, the rapid replacement of the chromatographic box 10 and the chromatographic column 120 is realized. The use of the chromatographic device in this embodiment can simplify the process of replacing the chromatographic column 120 and save the time of replacing the chromatographic column 120.

[0031] Furthermore, both ends of the chromatographic column 120 are connected to the adapter assembly 130 through a pipeline, and are communicated with the first channel 131 through a pipeline. In this way, the installation and removal of the chromatographic column 120 in the chromatographic box 10 can be facilitated, and the universality of the chromatographic column 120 to the chromatographic box 10 is improved.

[0032] It should also be noted that when the sample needle 221 is not inserted into the first channel 131 in the adapter assembly 130, the first channel 131 is closed. In this way, when the sample needle 221 is connected to the chromatographic column 120 through the first channel 131, it can be ensured that the sample will not leak in the adapter assembly 130 during the process of flowing into the chromatographic column 120.

[0033] The method of using the chromatographic device is as follows: the chromatographic box 10 is placed on the bracket 210, and the positioning component 230 on the bracket 210 positions the chromatographic box 10. After the chromatographic box 10 reaches the target position, when the sample needle 221 is moved to the first position, the interior of the sample needle 221 is connected to the interior of the chromatographic column 120 through the first channel 131 of the adapter component 130, thereby realizing the installation of the chromatographic column 120 on the chromatographic device. If there is a chromatographic box 10 with an old chromatographic column on the bracket 210 of the quick-insert and quick-change mechanism 20, it is necessary to first disassemble the chromatographic box 10 with the old chromatographic column. The disassembly method is: when the sample needle 221 is moved to the second position, the sample needle 221 is moved away from the adapter assembly 130, and the sample needle 221 is disconnected from the chromatographic column 120 inside the chromatographic box 10, and the chromatographic box 10 with the old chromatographic column is directly taken out from the bracket 210; if there is no chromatographic box 10 with an old chromatographic column on the bracket 210, that is, there is no need to remove the chromatographic box 10 with the old chromatographic column first, then the chromatographic box 10 with the new chromatographic column can be directly installed on the chromatographic device.

[0034] Furthermore, in this embodiment, the sample needle 221 can also be moved between the first position and the second position in an automated manner. Schematically, the positioning component 230 is connected to a programmable logic controller (PLC) for communication, and the positioning component 230 detects the position of the chromatographic box 10 in real time and uploads it to the PLC. After the positioning component 230 or the PLC determines that the chromatographic box 10 has reached the target position, the PLC controls the driving member 2231 (such as a motor or a cylinder) to move the sample needle 221 from the second position to the first position, and the sample needle 221 is connected to the chromatographic column 120 through the first channel 131 of the adapter component 130. Similarly, after the PLC receives an instruction that the sample needle 221 needs to move from the second position to the first position, the PLC controls the driving member 2231 to move the sample needle 221 from the first position to the second position, and disconnects the sample needle 221 from the chromatographic column 120, so that the sample needle 221 is separated from the first channel 131 of the adapter component 130. In other embodiments, the sample needle 221 may be moved between the first position and the second position manually, which is not limited in the present application. It should be explained that the target position is a position where the sample needle 221 can enter the first channel 131 of the adapter assembly 130 when the sample needle 221 is in the first position. The specific position of the target position on the quick-insert and quick-change mechanism 20 is not limited in this application.

[0035] In one embodiment, see Figure 2 , Figure 3 and Figure 4 The two ends of the adapter assembly 130 are respectively arranged on both sides of the side walls of the box body 110; the adapter assembly 130 includes a sealing member 132, a sealing mounting seat 133 and a sealing mounting cover 134, the sealing mounting seat 133 is penetrated through the side wall of the box body 110, the sealing mounting seat 133 and the sealing mounting cover 134 are detachably connected, and the sealing mounting seat 133 and the sealing mounting cover 134 enclose a sealed space, the sealing member 132 is arranged in the sealed space, and the sealing member 132 is provided with a first channel 131; when the sample needle 221 is in the first position, the sample needle 221 is penetrated through the sealing mounting cover 134, and the outer wall of the sample needle 221 is tightly fitted with the first channel 131. Even if the chromatographic column 120 is placed in the chromatographic box 10, the sample can enter the chromatographic column 120 through the sample needle 221 and the adapter assembly 130, and leave the chromatographic column 120 through another sample needle 221 and the adapter assembly 130, so that the chromatographic column 120 can be replaced by replacing the chromatographic box 10 in this embodiment. Moreover, when the sample needle 221 is connected to the chromatographic column 120 through the first channel 131, it is ensured that the sample will not leak in the adapter assembly 130 during the process of flowing into the chromatographic column 120, thereby ensuring the normal operation of the subsequent chromatographic device.

[0036] It is understandable that the sealing installation cover 134 is provided with a first avoidance hole 136, which is arranged corresponding to the first channel 131, and the sample needle 221 is inserted into the sealing installation cover 134 through the first avoidance hole 136. In the process of the sample needle 221 moving from the second position to the first position and inserting into the first channel 131, the sample needle 221 needs to pass through the first avoidance hole 136 before being inserted into the first channel 131.

[0037] It can also be understood that the sealing mounting seat 133 is provided with a second avoidance hole 137, and the second avoidance hole 137 is arranged corresponding to the first channel 131, and the pipeline connected to the chromatographic column 120 is passed through the second avoidance hole 137 and is arranged in the sealing mounting seat 133. When the pipeline is connected to the first channel 131, the pipeline needs to pass through the second avoidance hole 137 before being inserted into the first channel 131 and connected to the first channel 131.

[0038] Furthermore, the central axis of the first avoidance hole 136 , the central axis of the first channel 131 and the central axis of the second avoidance hole 137 are coaxial, so that when the sample needle 221 is in the first position, the sample can flow smoothly through the sample needle 221 and the adapter assembly 130 .

[0039] In one embodiment, the adapter assembly 130 further includes a sealing pressure ring 135, which is disposed between the sealing member 132 and the sealing mounting cover 134 to press the sealing member 132 against the sealing mounting seat 133. In this way, the position of the sealing member 132 can be stabilized to prevent the sealing member 132 from moving or deviating in the sealed space enclosed by the sealing mounting seat 133 and the sealing mounting cover 134, thereby ensuring that the central axis of the first channel 131 remains unchanged, so that the sample needle 221 can be accurately inserted into the first channel 131 each time it reaches the first position.

[0040] It can be understood that the third avoidance hole 138 is provided on the sealing pressure ring 135, the first avoidance hole 136, the third avoidance hole 138 and the first channel 131 are correspondingly provided, and the sample needle 221 is inserted into the sealing pressure ring 135 through the third avoidance hole 138. In the process of the sample needle 221 moving from the second position to the first position and inserting into the first channel 131, the sample needle 221 needs to first pass through the first avoidance hole 136 and the third avoidance hole 138 before being inserted into the first channel 131.

[0041] In one embodiment, see Figure 2 and Figure 3, a heating sub-box 140 is provided in the chromatographic box 10, a pressing plate 142 and a heating assembly 143 which are tightly fitted are provided in the heating sub-box 140, a fixing groove 1431 is provided between the pressing plate 142 and the heating assembly 143, the fixing groove 1431 is used to accommodate at least part of the chromatographic column 120, and the heating assembly 143 is used to heat the chromatographic column 120 accommodated in the fixing groove 1431. There is only one heating sub-box 140 in each chromatographic box 10, and only one chromatographic column 120 is provided in each heating sub-box 140. By providing the fixing groove 1431 between the pressing plate 142 and the heating assembly 143 as the temperature control space of the chromatographic column 120, and only one chromatographic column 120 is provided in each fixing groove 1431, the overall size of the heating assembly 143 is greatly reduced, and the temperature control space is made more compact. At the same time, a heating component 143 controls the temperature of a chromatographic column 120. When multiple chromatographic devices are used at the same time, each chromatographic column 120 can be individually temperature controlled. Since the chromatographic columns 120 are independent of each other, the temperature control of each chromatographic column 120 is more precise, avoiding temperature interference between multiple chromatographic columns 120, and making the chromatographic device's detection results of samples more stable and reliable.

[0042] In one embodiment, the chromatographic column 120 is connected to the adapter assembly 130 through a pipeline, the pipeline includes an injection pipeline 121, one end of the injection pipeline 121 is connected to the first channel 131 of one of the adapter assemblies 130, and the other end of the injection pipeline 121 is connected to the injection port of the chromatographic column 120. The fixed groove 1431 is also used to accommodate part of the injection pipeline 121, and the heating component 143 is also used to heat the part of the injection pipeline 121 accommodated in the fixed groove 1431 to preheat the sample in the injection pipeline 121. In this way, the temperature of the sample in the chromatographic column 120 can reach the target temperature faster, and the temperature of the sample in the chromatographic column 120 is made more accurate, thereby making the detection result of the sample by the chromatographic device more stable and reliable.

[0043] In one embodiment, the heating assembly 143 includes a heating element 1432 and a heat-conducting element 1433, one side of the heat-conducting element 1433 is connected to the heating element 1432, and the other side of the heat-conducting element 1433 is tightly matched with the pressing plate 142, and a fixed groove 1431 is surrounded between the pressing plate 142 and the heat-conducting element 1433, and the heating element 1432 is used to heat the chromatographic column 120 and part of the injection pipeline 121 accommodated in the fixed groove 1431. It can be understood that after the heating element 1432 generates heat, the heat is transferred to the heat-conducting element 1433, and the heat-conducting element 1433 then transfers the heat to the temperature-controlled space of the fixed groove 1431, thereby heating the chromatographic column 120 and part of the injection pipeline 121 in the fixed groove 1431.

[0044] In one embodiment, the heating assembly 143 further includes a temperature sensor 1434 and a temperature protection switch 1435. The temperature sensor 1434 is used to detect the temperature of the heat conducting member 1433. The temperature sensor 1434 and the heating member 1432 are both connected to the temperature protection switch 1435 for communication. The temperature protection switch 1435 is used to turn on or off the heating member 1432 according to the temperature detected by the temperature sensor 1434. In this way, accurate temperature control of the chromatographic column 120 in the heating sub-box 140 can be achieved.

[0045] Further, the heating sub-box 140 includes a base plate 141 and a cover 144, and the base plate 141 and the cover 144 are surrounded by an installation space, and the chromatographic column 120, the heating assembly 143 and the pressing plate 142 are all arranged in the installation space. And the heating assembly 143 is arranged on the base plate 141, and the base plate 141 is arranged on the side of the heating element 1432 away from the heat-conducting element 1433, and the base plate 141 and the cover 144 are detachably connected. After the cover 144 is removed from the base plate 141, the chromatographic column 120 can be replaced. The chromatographic column 120 and the heating assembly 143 are integrated in the heating sub-box 140, so that the setting of the heating sub-box 140 is more compact, saving the volume of the heating sub-box 140 and the chromatographic box 10.

[0046] It should be noted that the steps for replacing the chromatographic column 120 from the heating sub-box 140 are: remove the cover 144, remove the pressure plate 142, disconnect the two ends of the chromatographic column 120 from the pipeline, connect the two ends of the new chromatographic column 120 to the pipeline, place the chromatographic column 120 into the fixing groove 1431, connect the pressure plate 142, and then install the cover 144.

[0047] Furthermore, a pipe connecting the chromatographic column 120 and the adapter assembly 130 is passed through the cover 144 , so that the chromatographic column 120 can communicate with the first channel 131 of the adapter assembly 130 .

[0048] In one embodiment, a socket 113 is further provided in the box body 110, and a smart chip is provided in the socket 113, and the socket 113 is penetrated through the box body 110; a plug-in 212 is provided on the bracket 210, and when the chromatographic box 10 is positioned on the bracket 210 by the positioning component 230, the plug-in 212 is plugged into the socket 113. The smart chip is used to identify, read, load, and edit the chromatographic column information integrated in the chromatographic box 10, and the chromatographic column information includes but is not limited to the filler type, filler density, specification model, production batch, and validity period.

[0049] It is understandable that the method of identifying the chromatographic column information in the prior art is mostly to paste the corresponding barcode on the chromatographic column and then identify it through a barcode scanner. However, there are many problems with the method of using barcodes to identify chromatographic column information, such as the limited amount of information that can be stored, strong environmental dependence, low stability, poor material adaptability, and the need to be equipped with a corresponding infrared barcode scanner, which is costly; and the infrared barcode scanner has reading restrictions, and the barcode must be within the line of sight of the infrared barcode scanner to be able to scan. However, by setting up a smart chip, the present embodiment can realize the intelligent automatic identification, reading, and loading of the chromatographic column information by reading the smart chip. In this way, the smart chip in the present embodiment not only has a high storage capacity, can be recycled, and has a low cost, but also does not require a special scanning operation, and is simple to use. The identification and reading of the smart chip are both prior art, and the present application omits the description here.

[0050] Specifically, the connector 212 is arranged corresponding to the socket 113. When the chromatographic box 10 reaches the target position, the connector 212 is automatically plugged into the socket 113. In this way, the automation of the chromatographic device can be improved, and the replacement process of the chromatographic column 120 can be further simplified.

[0051] In one embodiment, a handle 114 is further provided on the box body 110 , which facilitates taking and placing the chromatography box 10 .

[0052] In one embodiment, participation Figure 6 The positioning assembly 230 is provided with a positioning groove 231, and the positioning groove 231 is used to accommodate the chromatographic box 10. The side of the positioning groove 231 is provided with a first opening 232, and the first opening 232 faces the sample inlet and outlet unit 220. It can be understood that when the chromatographic box 10 is placed in the positioning groove 231, the side wall of the box body 110 on which the adapter assembly 130 is passed faces the first opening 232, and the adapter assembly 130 is close to the first opening 232, so that when the sample needle 221 of the sample inlet and outlet unit 220 moves from the second position to the first position, the sample needle 221 can pass through the first opening 232 and be inserted into the first channel 131 of the adapter assembly 130, so that the sample needle 221 is connected with the chromatographic column 120.

[0053] Furthermore, a second opening 233 is provided at the upper end of the positioning groove 231. In this way, the chromatographic box 10 can be conveniently placed in the positioning groove 231 or conveniently taken out of the positioning groove 231. Furthermore, the extension direction of the first opening 232 intersects with the extension direction of the second opening 233. In this embodiment, the chromatographic box 10 can be placed in the positioning groove 231 or taken out of the positioning groove 231 through the first opening 232, the second opening 233, or the intersection of the first outlet and the second outlet, so that the placement and taking of the chromatographic box 10 is more convenient.

[0054] In one embodiment, the first opening 232 and the second opening 233 can be the same opening, and the present application does not impose any limitation thereto, as long as the opening allows the chromatography box 10 to enter the positioning groove 231 and does not affect the insertion of the sample needle 221 into the first channel 131 in the adapter assembly 130. Schematically, the opening is an opening that is tilted downward toward the sample inlet and outlet unit 220, so that the sample needle 221 can pass through the opening and be inserted into the first channel 131 of the adapter assembly 130, and the chromatography box 10 can be easily taken in and out.

[0055] In one embodiment, the positioning assembly 230 includes two positioning plates 234 arranged opposite to each other, a positioning groove 231 is formed between the two positioning plates 234, and a first plate 235 is provided on the side of the positioning groove 231 opposite to the first opening 232. It can be understood that the first channel 131 in the adapter assembly 130 is sealed, and the sample needle 221 can be inserted into the first channel 131 of the adapter assembly 130 only when a certain thrust is applied. The first plate 235 can provide a stop site for the chromatographic cartridge 10, and during the process of the sample needle 221 being inserted into the first channel 131, the chromatographic cartridge 10 can be prevented from moving along the extending direction of the positioning groove 231 due to the thrust of the sample needle 221 on the adapter assembly 130, thereby ensuring that the sample needle 221 smoothly enters the first channel 131 of the adapter assembly 130. Moreover, the positioning groove 231 can provide a limiting effect for the chromatographic cartridge 10, thereby preventing the chromatographic cartridge 10 from moving in the positioning groove 231.

[0056] Furthermore, the first channel 131 has at least a partially linear channel, and at least one end of the sample needle 221 close to the positioning groove 231 is linear. The positioning groove 231 can help align the central axis of the linear first channel 131 in the chromatographic box 10 with the central axis of the linear sample needle 221, thereby further ensuring that the sample needle 221 can smoothly enter the first channel 131 of the adapter assembly 130.

[0057] Specifically, the first plate 235 can be disposed on the bracket 210 , or on a side of the positioning plate 234 away from the first opening 232 . The present application does not impose any limitation on this, as long as the first plate 235 and the positioning plate 234 can enclose the positioning groove 231 .

[0058] In one embodiment, the length of the positioning groove 231 is longer than the length of the chromatographic box 10. In this way, sufficient operating space is provided for taking and placing the chromatographic box 10 in the positioning groove 231, making it more convenient to take and place the chromatographic box 10, and preventing the sample inlet and outlet unit 220 from being accidentally damaged when taking and placing the chromatographic box 10, especially preventing the sample needle 221 from being accidentally damaged when taking and placing the chromatographic box 10.

[0059] Furthermore, a guide rail 236 is provided at the bottom of the positioning groove 231, and a guide groove 111 is provided on the chromatographic box 10, and the guide groove 111 is slidably matched with the guide rail 236. In this way, the chromatographic box 10 can be pushed to the target position along the guide rail 236, so that the chromatographic box 10 can move in the positioning groove 231.

[0060] In one embodiment, see Figure 5 and Figure 6 The quick-insert and quick-change mechanism 20 further includes a pressing assembly 240, which includes a pressing member 241, a connecting member 242 and a first elastic member 243. One end of the connecting member 242 is rotatably connected to the bracket 210, and the other end of the connecting member 242 is connected to the pressing member 241. The first elastic member 243 acts on the connecting member 242 or the pressing member 241 to press the pressing member 241 against the chromatographic box 10. By applying pressure to the chromatographic box 10 through the pressing assembly 240, the position of the chromatographic box 10 in the positioning groove 231 can be further stabilized, thereby ensuring that the sample needle 221 can smoothly enter the first channel 131 of the adapter assembly 130, and ensuring that when the sample needle 221 leaves the first channel 131, the position of the chromatographic box 10 will not move.

[0061] The bottom surface of the pressing member 241 is flush with or slightly lower than the top surface of the chromatographic box 10. The position of the pressing member 241 when it is not in contact with the chromatographic box 10 is defined as the original position. It is understandable that in the process from the pressing member 241 just contacting the chromatographic box 10 to the chromatographic box 10 moving to the target position, the pressing member 241 will move in an arc shape with the connection between the connecting member 242 and the bracket 210 as the center and the connection between the pressing member 241 and the bracket 210 as the radius, so that the pressing member 241 will not hinder the chromatographic box 10 from reaching the target position smoothly. As the pressing member 241 moves away from the original position gradually with the movement of the chromatographic box 10, the first elastic member 243 pulls the pressing member 241 back to the original position or applies a pulling force to the pressing member 241 to return to the original position, so that the pressing member 241 can be ensured to always press against the chromatographic box 10, and the pressure applied by the pressing assembly 240 to the chromatographic box 10 is maximized, and the pressing effect of the pressing assembly 240 on the chromatographic box 10 is the best.

[0062] Schematically, the first elastic member 243 is a component such as a spring or a tension band that can store and release energy and maintain the contact pressure between the connecting member 242 and the chromatographic box 10. Specifically, the end of the connecting member 242 away from the pressing member 241 can be rotatably connected to the first plate 235, and can also be rotatably connected to the positioning plate 234. The present application does not limit this, as long as the pressing member 241 can be pressed against the chromatographic box 10.

[0063] In one embodiment, the pressing member 241 is rotatably connected to the connecting member 242. Thus, after the pressing member 241 contacts the chromatography box 10, the relative movement between the pressing member 241 and the chromatography box 10 is smoother, and the pressing member 241 will not hinder the movement of the chromatography box 10 in the positioning groove 231.

[0064] Specifically, the pressing member 241 is connected to the connecting member 242 via a shaft, the pressing member 241 is disposed on the shaft, and the pressing member 241 can rotate circumferentially around the shaft. Further, the pressing member 241 is a roller. It is understood that the above specific embodiments are all illustrative, and the present application does not limit this.

[0065] In one embodiment, in the height direction of the chromatographic box 10, a stopper 112 is convexly provided on the top surface of the chromatographic box 10, and when one end of the chromatographic box 10 abuts against the first plate 235, the pressing member 241 presses against the stopper 112. The stopper 112 can limit the pressing member 241, so that relative movement between the pressing member 241 and the chromatographic box 10 can be avoided, and the pressing effect of the pressing member 241 on the chromatographic box 10 can be improved.

[0066] Specifically, the limiting member 112 is wavy, and the limiting member 112 includes two wave crests and a trough clamped between the two wave crests. When one end of the chromatographic box 10 abuts against the first plate 235, the pressing member 241 presses against the trough, and the two wave crests can limit the pressing member 241, so that the pressing effect of the pressing member 241 on the chromatographic box 10 can be further improved, and relative movement between the pressing member 241 and the chromatographic box 10 can be avoided. When the wave crest close to the pressing member 241 reaches the position of the pressing member 241, the pressing member 241 moves along the surface of the wave crest and is lifted by the wave crest to the highest point of the wave crest, and then the pressing member 241 gradually decreases with the alternation of the wave crest and the pressure of the first elastic member 243, and finally presses against the position of the wave trough.

[0067] In one embodiment, the pressing assembly 240 further includes a shock absorbing pad 244, which is disposed between the upper end of the positioning plate 234 and the connecting member 242 in the height direction of the positioning plate 234, and the shock absorbing pad 244 is connected to the positioning plate 234, and the shock absorbing pad 244 abuts against the connecting member 242. It is understandable that when the chromatography box 10 is separated from the pressing member 241, the pressing member 241 will move toward the direction of the positioning groove 231 under the pressure of the first elastic member 243 and the action of gravity, and the shock absorbing pad 244 can prevent the pressing member 241 from touching the positioning plate 234, thereby stabilizing the positioning plate 234 and the positioning groove 231; and the shock absorbing pad 244 can also keep the pressing member 241 in place.

[0068] In one embodiment, the upper portion of the positioning plate 234 is provided with a first flange 2341 extending in a direction away from the positioning groove 231, and the shock absorbing pad 244 is connected to the first flange 2341. The first flange 2341 can increase the connection area between the positioning plate 234 and the shock absorbing pad 244, so that the connection of the shock absorbing pad 244 on the first flange 2341 is more stable, thereby allowing the shock absorbing pad 244 to better play a shock absorbing role. Moreover, when the chromatographic box 10 is placed in the positioning groove 231, the first flange 2341 can prevent the edge of the positioning plate 234 from cutting the chromatographic box 10.

[0069] Furthermore, a second flange 2342 is provided on the side of the positioning plate 234 away from the sample inlet and outlet unit 220, and the second flange 2342 is extended in a direction away from the positioning groove 231, and the second flange 2342 is connected to the edge of the first plate 235. That is to say, in the width direction of the positioning groove 231, there is a certain space between the side of the positioning plate 234 away from the positioning groove 231 and the edge of the first plate 235, and the first elastic member 243 can be placed in the space, so that the space can provide protection for the first elastic member 243, prevent the first elastic member 243 from being accidentally touched and falling off, thereby ensuring the stability of the tension applied by the first elastic member 243 to the pressing member 241, ensuring the pressing effect of the pressing assembly 240 on the chromatographic box 10, and making the quick-insert and quick-change mechanism 20 more compact.

[0070] Furthermore, a third flange 2343 is provided at the first opening 232 of the positioning plate 234 , and the third flange 2343 is extended in a direction away from the positioning groove 231 , and the third flange 2343 can provide a resting position for the sample inlet and outlet unit 220 .

[0071] In one embodiment, the bracket 210 is further provided with a first position detection member 211, which is used to detect the position of the chromatographic box 10, and the first position detection member 211 is in communication connection with the sample inlet and outlet unit 220. After the first position detection member 211 detects that the chromatographic box 10 has reached the target position, the sample inlet and outlet unit 220 can drive the sample needle 221 to move to the first position. In this way, the sample needle 221 can automatically move between the first position and the second position, thereby improving the automation of the chromatographic device and further simplifying the replacement process of the chromatographic column 120.

[0072] In one embodiment, the sample inlet and outlet unit 220 further includes a driving component 222 and a pushing component 223. The two sample needles 221 are both arranged in the pushing component 223. The driving component 222 drives the pushing component 223 to move, so as to drive the two sample needles 221 to move between the first position and the second position. The driving component 222 and the pushing component control the sample needles 221 to move between the first position and the second position, thereby improving the automation of the chromatographic device and further simplifying the replacement process of the chromatographic column 120.

[0073] In one embodiment, the propulsion assembly 223 includes a fixed plate 2221, a clamping plate 2222, a second elastic member 2223 and a guide shaft 2224. The fixed plate 2221 and the clamping plate 2222 are arranged opposite to each other, one end of the sample needle 221 is fixed to the fixed plate 2221, and the other end of the sample needle 221 is inserted through the clamping plate 2222. The guide shaft 2224 is movably inserted through the fixed plate 2221, one end of the guide shaft 2224 is fixedly connected to the clamping plate 2222, and the other end of the guide shaft 2224 is provided with a stopper 2225, which stops at one side of the fixed plate 2221 to limit the guide shaft 2224 from being separated from the fixed plate 2221. The fixed plate 2221 can move along the axial direction of the guide shaft 2224 under the drive of the driving member 2231, and drive the sample needle 221 to move between the first position and the second position. The second elastic member 2223 is sleeved on the guide shaft 2224, and the two ends of the second elastic member 2223 are respectively pressed against the fixed plate 2221 and the pressing plate 2222, so that the stopper 2225 is pressed against the side of the fixed plate 2221 away from the pressing plate 2222. In this way, the linear motion of the sample needle 221 can be ensured, making it easier for the sample needle 221 to be inserted into the first channel 131 of the adapter assembly 130. In addition, within a certain position range, the second elastic member 2223 can make the pressing plate 2222 move synchronously with the fixed plate 2221, so that the pressing plate 2222 and the fixed plate 2221 remain relatively still and stable, so as to further stabilize the motion of the sample needle 221.

[0074] It can be understood that the axial direction of the guide shaft 2224 is the same as the direction in which the sample needle 221 moves between the first position and the second position. Driven by the driving assembly 222, the fixed plate 2221 can move along the axial direction of the guide shaft 2224, that is, move in a direction close to or away from the adapter assembly 130. Since the second elastic member 2223 is sleeved on the guide shaft 2224, and the two ends of the second elastic member 2223 are respectively abutted against the fixed plate 2221 and the clamping plate 2222, when the fixed plate 2221 moves in a direction close to the adapter assembly 130, it can also drive the clamping plate 2222 to move in a direction close to the adapter assembly 130, and can also drive the sample needle 221, the second elastic member 2223 and the guide shaft 2224 to move in a direction close to the adapter assembly 130. From the above, it can be seen that the adapter assembly 130 is arranged in the positioning groove 231 surrounded by the positioning plate 234, and the side of the positioning groove 231 is provided with a first opening 232 facing the clamping plate 2222. After the clamping plate 2222 abuts against the first opening 232, the clamping plate 2222 and the guide shaft 2224 cannot continue to move toward the adapter assembly 130, while the fixed plate 2221 continues to move toward the direction close to the adapter assembly 130. At this time, the fixing plate 2221 is separated from the stopper 2225, and the fixing plate 2221 begins to squeeze the second elastic member 2223, causing the second elastic member 2223 to enter a compressed state from the original state, and the end of the sample needle 221 close to the adapter assembly 130 begins to move relative to the clamping plate 2222, and the end of the sample needle 221 gradually moves away from the clamping plate 2222 and gradually approaches the adapter assembly 130 until it is inserted into the first channel 131 of the adapter assembly 130, and the fixing plate 2221 stops moving toward the direction close to the adapter assembly 130.

[0075] When the fixing plate 2221 moves from the above position toward the direction away from the adapter assembly 130 driven by the driving assembly 222, there is a relative movement between the end of the sample needle 221 close to the adapter assembly 130 and the pressing plate 2222, and the end of the sample needle 221 is separated from the first channel 131 of the adapter assembly 130, and the end gradually moves away from the adapter assembly 130 and gradually approaches the pressing plate 2222, until the second elastic member 2223 gradually recovers from the compressed state to the original state, and the fixing plate 2221 abuts against the stopper 2225 of the guide shaft 2224. Then, the fixing plate 2221 continues to move toward the direction away from the adapter assembly 130 driven by the driving assembly 222, and at this time, the fixing plate 2221 can simultaneously drive the sample needle 221, the pressing plate 2222, the second elastic member 2223 and the guide shaft 2224 to move toward the direction away from the adapter assembly 130.

[0076] Furthermore, a third flange 2343 is provided at the first opening 232 of the positioning plate 234, that is, a third flange 2343 is provided at the end of the positioning plate 234 close to the pressing plate 2222. The pressing plate 2222 can abut against the third flange 2343. The third flange 2343 increases the contact area between the pressing plate 2222 and the first opening 232 of the positioning plate 234, so that a better abutment position can be provided for the pressing plate 2222, and the edge of the positioning plate 234 can be prevented from cutting the pressing plate 2222.

[0077] In one embodiment, a groove 2226 is provided on one side of the pressing plate 2222 close to the adapter assembly 130; the adapter assembly 130 can be accommodated in the groove 2226. The outer wall of the adapter assembly 130 abuts against a portion of the outer circumference of the sealing mounting cover 134 of the adapter assembly 130, so that the central axis of the sample needle 221 and the central axis of the first channel 131 of the adapter assembly 130 are coaxial, so that the sample needle 221 can accurately enter the first channel 131.

[0078] In one embodiment, the driving assembly 222 includes a driving member 2231, a screw rod 2232, a slider 2233 and a first guide rail 2234. The slider 2233 is slidably matched with the first guide rail 2234. The fixing plate 2221 is connected to the slider 2233. The driving member 2231 is used to drive the screw rod 2232 to rotate, so that the slider 2233 moves along the axial direction of the screw rod 2232, and the fixing plate 2221 drives the sample needle 221 to move between the first position and the second position. In this way, the driving member 2231 can drive the sample needle 221 to move between the first position and the second position.

[0079] It can be understood that the axial direction of the screw rod 2232 is the same as the axial direction of the guide shaft 2224. Specifically, the driving member 2231 is a motor, and the central axis of the output shaft of the motor is coaxial with the central axis of the screw rod 2232. In this way, the motor can be directly connected to the screw rod 2232 through its output shaft, without using a transmission member to transmit the driving force of the motor to the screw rod 2232, thereby saving costs.

[0080] In other embodiments, the driving member 2231 is a motor, the central axis of the output shaft of the motor is not coaxial with the central axis of the screw rod 2232, and the driving assembly 222 further includes a transmission group 2235, the transmission member connects the motor and the screw rod 2232, and the motor transmits its own driving force to the screw rod 2232 through the transmission member. In this way, the position of the motor can be set arbitrarily, thereby improving the flexibility and universality of the chromatographic device.

[0081] In one embodiment, the sample inlet and outlet unit 220 further includes a mounting seat 224, and the first guide rail 2234 is disposed on the mounting seat 224. In this way, the support for the first guide rail 2234 is increased; and the central axis of the sample needle 221 can be made coaxial with the central axis of the first channel 131 of the adapter assembly 130 by adjusting the height of the mounting seat 224.

[0082] In one embodiment, the sample inlet and outlet unit 220 further includes a second position detection member 225, which is used to detect the position of the fixed plate 2221. The second position detection member 225 is in communication connection with the PLC and the driving member 2231. Through the position of the fixed plate 2221 detected by the second position detection member 225, the PLC controls the driving member 2231 to start driving the fixed plate 2221 to move or stop the fixed plate 2221 from moving. Furthermore, the second position detection member 225 is a photoelectric switch and a baffle used in conjunction with the photoelectric switch. The baffle is arranged on the side of the fixed plate 2221 away from the clamping plate 2222, and the photoelectric switch is arranged on the bracket 210.

[0083] In one embodiment, the end of the sample needle 221 close to the adapter component 130 is in a contracted shape, and the end of the sample needle 221 close to the adapter component 130 is defined as the first point, and the position near the end is defined as the second point, and the sample needle 221 gradually shrinks from the second point toward the first point. In this way, it can be more convenient to insert into the first channel 131 of the adapter component 130.

[0084] The present application also provides a chromatography system, comprising the chromatography device as described in any of the above embodiments.

[0085] In the prior art, multiple chromatographic columns are placed in the same column incubator, and the column incubator needs to be equipped with a large heating element, which makes the incubator and the chromatographic system relatively large in size and high in cost. Moreover, when a chromatographic column in the column incubator needs to be replaced, the door or cover of the column incubator needs to be opened, and it takes a certain amount of time to replace a chromatographic column, which will cause temperature fluctuations in the entire column incubator, thereby affecting the temperature control of other normally operating chromatographic columns and the stability and reliability of the test results.

[0086] In this embodiment, multiple chromatographic devices can be arranged at the same time, and each chromatographic device only includes one chromatographic column 120. In this way, each chromatographic column 120 can be individually temperature-controlled. Moreover, since the chromatographic columns 120 are independent of each other, the temperature control of each chromatographic column 120 is more precise, avoiding temperature interference between multiple chromatographic columns 120, and making the detection results of the chromatographic device on the sample more stable and reliable.

[0087] The present application also provides a method for replacing a chromatographic column, the replacement method being used for replacing a chromatographic column in a chromatographic device in any of the above embodiments, wherein a chromatographic box comprises a first chromatographic box and a second chromatographic box, the first chromatographic box being a chromatographic box positioned on a support of the chromatographic device before the chromatographic column is replaced, and the second chromatographic box being a chromatographic box positioned on a support of the chromatographic device after the chromatographic column is replaced, The replacement method includes the following: The sample needle moves from the first position to the second position, and the sample needle is separated from the adapter assembly of the first chromatographic box; Taking out the first chromatography box from the support; positioning a second chromatography cartridge on the support; The sample needle moves from the second position to the first position, the sample needle is inserted into the first channel of the adapter assembly of the second chromatographic box, and the sample needle is communicated with the first channel of the second chromatographic box, thereby completing the replacement of the chromatographic column.

[0088] It is understandable that before installing the second chromatographic box in the chromatographic device, it is necessary to install the new chromatographic column in the second chromatographic box in advance, and connect the new chromatographic column to the adapter assembly. Because when preparing the second chromatographic box, there is no need to suspend the operation of the chromatographic device, and it can be prepared before removing the first chromatographic box. For the chromatographic device, when the sample needle is moved to the second position, the sample needle and the adapter assembly are separated, and the first chromatographic box is directly taken out, and then the second chromatographic box is placed in the target position. When the sample needle is moved to the first position, the inside of the sample needle is connected to the first channel, so that the chromatographic box and the chromatographic column are quickly replaced. The present application can simplify the process of replacing the chromatographic column and save time for replacing the chromatographic column by integrating the chromatographic column in the chromatographic box and quickly replacing the chromatographic column by replacing the chromatographic box.

[0089] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0090] The above-described embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the scope of patent protection of the present application shall be subject to the attached claims.

Claims

1. A chromatographic device, characterized in that It comprises a chromatographic box (10) and a quick-insert and quick-change mechanism (20). The chromatographic box (10) comprises a box body (110) and a chromatographic column (120) disposed in the box body (110), both ends of the chromatographic column (120) being connected to adapter components (130), the two adapter components (130) being disposed through the side wall of the box body (110), the adapter component (130) being provided with a first channel (131), the first channel (131) being in communication with the interior of the chromatographic column (120), The quick-insert and quick-change mechanism (20) comprises a bracket (210) and a sample inlet and outlet unit (220) disposed on the bracket (210); the bracket (210) is provided with a positioning component (230); the positioning component (230) is used to position the chromatographic box (10); The sample inlet and outlet unit (220) comprises two movable sample needles (221), and the two sample needles (221) are used to be connected to two adapter components (130) respectively. When the chromatographic box (10) is positioned on the bracket (210) by the positioning component (230), the sample needle (221) can move between a first position and a second position. When the sample needle (221) moves to the first position, the sample needle (221) is inserted into the first channel (131) of the adapter component (130) so that the interior of the sample needle (221) is connected to the first channel (131). When the sample needle (221) moves to the second position, the sample needle (221) and the adapter component (130) are separated.

2. The chromatographic device according to claim 1, characterized in that The quick-insert and quick-change mechanism (20) further comprises a clamping assembly (240), wherein the clamping assembly (240) comprises a clamping member (241), a connecting member (242) and a first elastic member (243). One end of the connecting member (242) is rotatably connected to the bracket (210), and the other end of the connecting member (242) is connected to the pressing member (241). The first elastic member (243) acts on the connecting member (242) or the pressing member (241) to press the pressing member (241) against the chromatography box (10).

3. The chromatographic device according to claim 1, characterized in that Two ends of the adapter component (130) are respectively arranged on two sides of the side wall of the box body (110); The adapter assembly (130) comprises a sealing member (132), a sealing mounting seat (133) and a sealing mounting cover (134); the sealing mounting seat (133) is disposed through a side wall of the box body (110); the sealing mounting seat (133) and the sealing mounting cover (134) are detachably connected; the sealing mounting seat (133) and the sealing mounting cover (134) enclose a sealed space; the sealing member (132) is disposed in the sealed space; and the sealing member (132) is provided with the first channel (131); When the sample needle (221) is in the first position, the sample needle (221) is inserted into the sealing installation cover (134), and the outer wall of the sample needle (221) is tightly fitted with the first channel (131).

4. The chromatographic device according to claim 3, characterized in that The adapter assembly (130) further comprises a sealing pressure ring (135), wherein the sealing pressure ring (135) is arranged between the sealing member (132) and the sealing installation cover (134) so ​​as to press the sealing member (132) against the sealing installation seat (133).

5. The chromatographic device according to claim 1, characterized in that The chromatography box (10) is provided with a heating sub-box (140), and the heating sub-box (140) is provided with a tightly fitting pressing plate (142) and a heating component (143), a fixing groove (1431) is provided between the pressing plate (142) and the heating component (143), and the fixing groove (1431) is used to accommodate at least a part of the chromatography column (120), and the heating component (143) is used to heat the chromatography column (120) accommodated in the fixing groove (1431).

6. The chromatographic device according to claim 5, characterized in that The chromatographic column (120) is connected to the adapter assembly (130) via a pipeline, wherein the pipeline comprises an injection pipeline (121), one end of the injection pipeline (121) is connected to a first channel (131) of one of the adapter assemblies (130), and the other end of the injection pipeline (121) is connected to an injection port of the chromatographic column (120), the fixed groove (1431) is further used to accommodate part of the injection pipeline (121), and the heating assembly (143) is further used to heat the part of the injection pipeline (121) accommodated in the fixed groove (1431).

7. The chromatographic device according to claim 5, characterized in that The heating assembly (143) comprises a heating element (1432), a heat conducting element (1433), a temperature sensor (1434) and a temperature protection switch (1435); one side of the heat conducting element (1433) is connected to the heating element (1432); the other side of the heat conducting element (1433) is tightly fitted to the pressing plate (142); the fixing groove (1431) is arranged between the pressing plate (142) and the heat conducting element (1433); The heating element (1432) is used to heat the chromatographic column (120) accommodated in the fixing groove (1431); The temperature sensor (1434) is used to detect the temperature of the heat conducting member (1433); The temperature sensor (1434) and the heating element (1432) are both communicatively connected to the temperature protection switch (1435), and the temperature protection switch (1435) is used to turn on or off the heating element (1432) according to the temperature detected by the temperature sensor (1434).

8. The chromatographic device according to claim 1, characterized in that A socket (113) is also provided in the box body (110), a smart chip is provided in the socket (113), and the socket (113) is inserted through the box body (110); The support (210) is provided with a plug-in component (212), and when the chromatography box (10) is positioned on the support (210) by the positioning component (230), the plug-in component (212) is plugged into the socket (113).

9. A chromatography system, characterized in that: Comprising a chromatography device as claimed in any one of claims 1 to 8.

10. A method for replacing a chromatographic column, characterized in that: The replacement method is used to replace a chromatographic column in a chromatographic device according to any one of claims 1 to 8, wherein the chromatographic box comprises a first chromatographic box and a second chromatographic box, wherein the first chromatographic box is a chromatographic box positioned on the support of the chromatographic device before the chromatographic column is replaced, and the second chromatographic box is a chromatographic box positioned on the support of the chromatographic device after the chromatographic column is replaced. The replacement method includes the following contents: The sample needle moves from the first position to the second position, and the sample needle is separated from the adapter component of the first chromatographic box; taking the first chromatography box out of the support; positioning the second chromatography cartridge on the support; The sample needle moves from the second position to the first position, the sample needle is inserted into the first channel of the adapter assembly of the second chromatographic box, and the sample needle is communicated with the first channel of the second chromatographic box, thereby completing the replacement of the chromatographic column.

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