Seed selection and hybridized variety production method of sabbage type rape micro-powder less cytoplasam male sterile series
A technology of Brassica napus and CMS, applied in botany equipment and methods, applications, plant gene improvement, etc., can solve problems such as lack of restorer lines, dysplasia of nectary glands, yellowing of leaves, etc.
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[0020] The present invention will be described in further detail below in conjunction with specific examples.
[0021] 1. Breeding of CMS lines:
[0022] Use ledos (Germany, double low) as the female parent and Zhongshuang 2 (China, double low) as the male parent for crossing. Five male sterile plants were found in the progeny population of this combination. The petals of this sterile material are small, and the stamens are brown, similar to nuclear sterility. Obviously, but the flowers that bloom in the later period are very sterile, and the artificial buds are self-fertilized without fruiting. The sterility was maintained with materials from different sources, and the results showed that the maintainer lines of Polima and Shaanxi 2A CMS could well maintain the sterility of 212A, and all the transgenic CMS lines had no trace pollen production, and These sterile lines all had the phenomenon of dead buds at the initial flowering stage. The CMS line 212A with strong growth po...
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