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Duplication method of rat acute or persistent hyperuricemia model

A technology of hyperuricemia and replication methods, which is applied in the field of replication of rat acute or persistent hyperuricemia models, and can solve the problems of unfavorable gout model replication, long replication cycle, and long time

Inactive Publication Date: 2005-11-23
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are two shortcomings in the above methods: first, it takes a long time to complete the replication of the model; second, the uric acid level of the replicated hyperuricemia model is mostly lower than the saturation concentration of uric acid crystals
The above deficiencies will bring more difficulties to the research and treatment of hyperuricemia and gout drugs: the replication cycle is long; the use of existing models can only observe the therapeutic effect of drugs on mild hyperuricemia; the level of high uric acid is lower than that produced by uric acid Saturating concentrations of deposition, which would be detrimental to the replication of gout models, etc.

Method used

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Experimental program
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Effect test

Embodiment 1

[0036] The replication method of embodiment 1. rat acute hyperuricemia model, its method is as follows:

[0037] Select male healthy Wistar rats with a body weight of 250g±10g, and infuse xanthine 500mg·kg by intragastric administration at room temperature -1 , inject OA 200mg·kg by intraperitoneal injection -1 .

Embodiment 2

[0038] The replication method of embodiment 2. rat acute hyperuricemia model, its method is as follows:

[0039] 1. Select male healthy Wistar rats with a body weight of 250g±10g, and infuse xanthine 500mg·kg by intragastric administration at room temperature -1 , inject OA 100mg·kg by intraperitoneal injection -1 ;

[0040] After 2.5 hours, intraperitoneal injection of OA 100mg·kg again -1 .

Embodiment 3

[0041] The replication method of embodiment 3. rat acute hyperuricemia model, its method step is as follows:

[0042] 1. Select male healthy Wistar rats with a body weight of 250g±10g, and infuse xanthine 500mg·kg by intragastric administration at room temperature -1 , inject OA 200mg·kg by intraperitoneal injection -1 ;

[0043] After 2.5 hours, give xanthine 500mg·kg again by intragastric administration -1 .

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Abstract

The invention discloses a duplication method of rat acute or persistent hyperuricemia model which comprises, (1) selecting male, female healthy rats with the specie of SD and Wistar and 150-450g of body weight, simultaneously administering purines by 100-2000 mg / kg and uric acid depressant by 5-1000mg / kg through stomach filling, hypodermic injection or abdominal cavity injection, (2) after 5-24 hours, administering the rats by the same mode and medicament dose, (3) administering the rats daily continually according to the step (1) and (2), continuing administration for one to two weeks.

Description

1. Technical field [0001] The invention relates to a replication method of an animal model, in particular to a replication method of a rat acute or persistent hyperuricemia model. 2. Background technology [0002] Gout is a group of heterogeneous diseases caused by purine metabolic disorders or (and) decreased uric acid excretion. Clinically, hyperuricemia is the main feature. Hyperuricemia is the biochemical basis of gout, and gout must be accompanied by hyperuricemia. Essentially, there is no strict boundary between hyperuricemia and gout. In recent years, research on the pathogenesis of gout and its drug treatment has become one of the hotspots in the medical field. At present, there is still a lack of mature animal models of gout at home and abroad, and the establishment of animal models of hyperuricemia is a prerequisite for gout research. There are still many imperfections in the existing models, which brings great inconvenience to the research of gout. The followin...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 徐立时乐
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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