Multiplex nucleic acid reactions

a nucleic acid and multi-nucleic acid technology, applied in the field of multi-nucleic acid reaction, can solve the problem of preparing samples for high-density chips, and achieve the effect of improving the stability and stability of the reaction

Inactive Publication Date: 2010-01-21
ILLUMINA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides methods for detecting target sequences in a sample. These methods involve using probes that hybridize to the target sequences and are modified to form amplification templates. The templates are then detected using a specific priming sequence and an extension enzyme. The methods can be used with a single sample or multiple samples, and can detect target sequences with high specificity and sensitivity. The technical effects of the invention include improved accuracy and efficiency in detecting target sequences, as well as simplified and automated procedures for detecting target sequences.

Problems solved by technology

However, sample preparation for these high density chips remains an issue.

Method used

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Embodiment Construction

[0032]The present invention is directed to a variety of compositions and methods directed to multiplexed analysis of nucleic acids. In a preferred embodiment the methods are directed to multiplexing of nucleic acid detection, genotyping and amplification reactions. While a large body of literature and methods exist for the use of high density biochips comprising nucleic acids, the preparation of samples containing target sequences to place on the biochips has not been significantly multiplexed to allow true high throughput methodologies. The present invention is directed to the use of a variety of methods that allow the multiplexed amplification of target sequences prior to detection by any of a variety of methods including placement on an array for detection, mass spectrometry, electrophoretic techniques, FACS analysis, and the like.

[0033]In general, the method includes a complexity reduction component, a specificity step and an amplification step. Preferably complexity reduction i...

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Abstract

The invention is directed to a variety of multiplexing methods used to amplify and / or genotype a variety of samples simultaneously.

Description

[0001]The present application is a continuation of U.S. application Ser. No. 10 / 194,958, filed on Jul. 12, 2002, which is a continuation-in-part of U.S. application Ser. No. 10 / 177,727, filed Jun. 20, 2002, which claims the benefit of priority to U.S. Provisional Application Nos. 60 / 305,118, filed Jul. 12, 2001, 60 / 311,271, filed Aug. 9, 2001, 60 / 336,958, filed Dec. 3, 2001 and 60 / 341,827, filed Dec. 17, 2001, and is a continuation-in-part of each of the following:[0002]U.S. application Ser. No. 09 / 779,376, filed Feb. 7, 2001, now abandoned, which claims the benefit of priority to U.S. Provisional Application Ser. Nos. 60 / 180,810, filed Feb. 7, 2000 and 60 / 234,732, filed Sep. 22, 2000;[0003]U.S. application Ser. No. 09 / 915,231, filed Jul. 24, 2001, now U.S. Pat. No. 6,890,741, which claims the benefit of priority to U.S. Provisional Application Ser. Nos. 60 / 234,143, filed Sep. 21, 2000 and 60 / 297,609, filed on Jun. 11, 2001 and is a continuation-in-part of U.S. application Ser. No. ...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C12Q1/68C12P19/34
CPCC12Q1/6834Y10T436/143333C12Q1/6837C12Q2525/155C12Q2563/131C12Q2563/179C12Q1/6876C12Q2600/16
InventorOLIPHANT, ARNOLDSTUELPNAGEL, JOHN R.CHEE, MARK S.BUTLER, SCOTT L.FAN, JIAN-BINGSHEN, MIN-JUI RICHARD
OwnerILLUMINA INC