Polypeptide powder of crocodile and preparation method and application thereof

Abstract

The invention relates to a polypeptide powder of crocodile and a preparation method and application thereof, which relate to a crocodile product. The method comprises the following steps of: cleaning the crocodile meat, removing adipose tissues, cutting the crocodile meat into pieces; adding water in the crocodile meat pieces, boiling the crocodile meat pieces in a boiled water bath, homogenatingto obtain homogenated liquid of crocodile meat; adding trypsin in the obtained homogenated liquid, carrying out water bath and enzymolysis to obtain an enzymolytic mixed liquid A; placing the enzymolytic mixed liquid A in the boiled water bath, inactivating enzyme to obtain an enzymolytic mixed liquid B; cooling the obtained enzymolytic mixed liquid B to room temperature and filtering to obtain the crocodile meat enzymolytic liquid; and drying the crocodile meat enzymolytic liquid to obtain the polypeptide powder of crocodile. The polypeptide powder of crocodile has a certain function of resisting fatigue and improving the exercise ability of the body and can be applied to the preparation of medicines for resisting antifatigue and improving body exercise ability.

Description

technical field

[0001] The invention relates to a crocodile product, in particular to a crocodile polypeptide powder and a preparation method and application thereof. Background technique

[0002] As an essential nutrient for the human body, protein is extremely important to the life and health of the human body. Epidemiological survey results show that if the daily protein intake is too low, it is easy to suffer from esophageal cancer, gastric cancer, liver cancer and so on. Experts from the World Health Organization and the Food and Agriculture Organization of the United Nations believe that the daily protein intake of an ordinary citizen should not be less than 75g in a standard diet for normal activities. According to the source of protein, it is divided into animal protein, vegetable protein, single cell protein and algae protein. These macromolecular proteins can supplement the proteins needed by the human body to a certain extent, but they have disadvantages such as...

Examples

Embodiment 1

[0029] The preparation of embodiment 1 crocodile polypeptide powder

[0030] 1) Weigh 2-5kg crocodile meat, wash and remove fat tissue, and cut into 1-2cm in size 3 of pieces.

[0031] 2) According to the volume ratio, add 2:5 distilled water, boil in a boiling water bath for 30 minutes, and then homogenize in a tissue grinder to obtain crocodile meat homogenate.

[0032] 3) Add trypsin accounting for 0.4% of the mass of crocodile meat to the crocodile meat homogenate obtained in step 2), mix thoroughly, and enzymolyze it in a water bath at 40-45°C for 4-6 hours, stirring once every 20 minutes .

[0033] 4) Put the enzymolysis mixture obtained in step 3) in a boiling water bath for 10-20 minutes to inactivate the enzyme.

[0034] 5) Put the mixed liquid obtained in step 4) and the enzymatic hydrolysis solution into the crocodile meat enzymatic hydrolysis solution after standing and naturally cooling to room temperature, and then filtering with 6-8 layers of gauze.

[0035]...

Embodiment 2

[0037] Composition determination of embodiment 2 crocodile polypeptide powder

[0038] A certain amount of crocodile polypeptide powder was weighed, redissolved in distilled water to prepare a 200mg / mL mother solution, and the protein concentration of the mother solution was determined by the Coomassie brilliant blue method, with bovine serum albumin as the standard; the Folin-phenol method was used to determine the polypeptide content of the mother solution, with Bovine serum albumin is standard substance; Adopt ninhydrin colorimetric method to measure the concentration of mother liquor free amino acid, take glycine as standard substance; Content adopts the total sugar concentration of mother liquor measured by sulfuric acid-phenol method, take glucose as standard substance; Calculate Protein, peptide, free amino acid, percentage of total sugar content.

[0039] Percent fat content determined by Soxhlet extraction. The percentage of moisture content was determined by atmosph...

Embodiment 3

[0041] The metal element content determination of embodiment 3 crocodile polypeptide powder

[0042]Weigh 0.2g of crocodile polypeptide powder into a microwave digestion bottle, add 5mL of concentrated nitric acid, 1mL of concentrated perchloric acid, and place it in a microwave digestion chamber for digestion. After digestion, dilute to 100mL with double distilled water, and use atomic absorption its metal element content.

technical field

[0001] The invention relates to a crocodile product, in particular to a crocodile polypeptide powder and a preparation method and application thereof. Background technique

[0002] As an essential nutrient for the human body, protein is extremely important to the life and health of the human body. Epidemiological survey results show that if the daily protein intake is too low, it is easy to suffer from esophageal cancer, gastric cancer, liver cancer and so on. Experts from the World Health Organization and the Food and Agriculture Organization of the United Nations believe that the daily protein intake of an ordinary citizen should not be less than 75g in a standard diet for normal activities. According to the source of protein, it is divided into animal protein, vegetable protein, single cell protein and algae protein. These macromolecular proteins can supplement the proteins needed by the human body to a certain extent, but they have disadvantages such as...

Examples

Embodiment 1

[0029] The preparation of embodiment 1 crocodile polypeptide powder

[0030] 1) Weigh 2-5kg crocodile meat, wash and remove fat tissue, and cut into 1-2cm in size 3 of pieces.

[0031] 2) According to the volume ratio, add 2:5 distilled water, boil in a boiling water bath for 30 minutes, and then homogenize in a tissue grinder to obtain crocodile meat homogenate.

[0032] 3) Add trypsin accounting for 0.4% of the mass of crocodile meat to the crocodile meat homogenate obtained in step 2), mix thoroughly, and enzymolyze it in a water bath at 40-45°C for 4-6 hours, stirring once every 20 minutes .

[0033] 4) Put the enzymolysis mixture obtained in step 3) in a boiling water bath for 10-20 minutes to inactivate the enzyme.

[0034] 5) Put the mixed liquid obtained in step 4) and the enzymatic hydrolysis solution into the crocodile meat enzymatic hydrolysis solution after standing and naturally cooling to room temperature, and then filtering with 6-8 layers of gauze.

[0035]...

Embodiment 2

[0037] Composition determination of embodiment 2 crocodile polypeptide powder

[0038] A certain amount of crocodile polypeptide powder was weighed, redissolved in distilled water to prepare a 200mg / mL mother solution, and the protein concentration of the mother solution was determined by the Coomassie brilliant blue method, with bovine serum albumin as the standard; the Folin-phenol method was used to determine the polypeptide content of the mother solution, with Bovine serum albumin is standard substance; Adopt ninhydrin colorimetric method to measure the concentration of mother liquor free amino acid, take glycine as standard substance; Content adopts the total sugar concentration of mother liquor measured by sulfuric acid-phenol method, take glucose as standard substance; Calculate Protein, peptide, free amino acid, percentage of total sugar content.

[0039] Percent fat content determined by Soxhlet extraction. The percentage of moisture content was determined by atmosph...

Embodiment 3

[0041] The metal element content determination of embodiment 3 crocodile polypeptide powder

[0042]Weigh 0.2g of crocodile polypeptide powder into a microwave digestion bottle, add 5mL of concentrated nitric acid, 1mL of concentrated perchloric acid, and place it in a microwave digestion chamber for digestion. After digestion, dilute to 100mL with double distilled water, and use atomic absorption its metal element content.

Images