Nerve conduit and preparation method thereof

Abstract

The invention discloses a nerve conduit and a preparation method thereof. The nerve conduit comprises an inner layer, a transition layer, an outer layer and at least one cavity used for storing a bioactive factor solution, wherein the outer surface of the inner layer is sequentially coated with the transition layer and the outer layer; the cavity is positioned between the inner surface of the outer layer and the outer surface of the transition layer and / or between the outer surface of the inner layer and the inner surface of the transition layer; the inner layer is a hydrophilic cytoskeleton layer made by adopting an electrostatic spinning method; and the outer layer is a hydrophobic nerve conduit scaffold layer made by adopting the electrostatic spinning method. The nerve conduit can be used for loading bioactive factors before an operation in a required mode, such as injection, soaking and the like due to the miniature cavity, so that the production quality of the product is easy to control, the survival rate of the bioactive factor can be greatly improved, the nerve regeneration can be promoted in high efficiency, and the nerve repairing effect can be reinforced.

Description

technical field

[0001] The invention belongs to the field of biomedical materials and implantable medical devices, and in particular relates to a nerve guide and a preparation method thereof. This case is a divisional application of the original application "CN201110073822.9 A nerve guide and its preparation method". Background technique

[0002] The nervous system is one of the most important organs in the human body, which controls the sensory and motor functions of the human body. Various traumas such as compression, stretching, tearing, cutting, and other factors such as ischemia, tumors, etc. will cause partial or complete damage to the nervous system, resulting in loss of function and other neurological diseases. The repair and functional reconstruction of defective nerves is one of the difficult problems to be overcome by human beings.

[0003] The neuronal axons of peripheral nerves can extend under appropriate conditions and grow longer than the injured area to ac...

Examples

Embodiment 1

[0105] (1) Select hydrophilic gelatin and silk fibroin, the mass ratio of the two is 70:30, the solvent is formic acid, the mass fraction of the spinning solution is 15%, and the spinning solution is added to the syringe of the electrospinning device, and the micro amount is adjusted The speed of the syringe pump is 8 ml / hour, the receiving distance is 10 cm, and the voltage is 20 kV. Turn on the electrospinning machine and perform spinning to obtain the inner layer of the cell scaffold with an average fiber diameter of 800-1000 nm;

[0106] (2) Select hydrophobic L-polylactic acid and ε-caprolactone copolymerized polymer materials, the mass ratio of the two is 50:50, the number average molecular weight is 310,000, and the mass ratio of gelatin and silk fibroin is 70:30 As a solute, it is dissolved in hexafluoroisopropanol to obtain a spinning solution with a mass fraction of 12%, and based on the mass ratio of (L-polylactic acid and ε-caprolactone: gelatin and silk fibroin) 25...

Embodiment 2

[0110] (1) Select hydrophilic chondroitin sulfate and hyaluronic acid, the mass ratio of the two is 70:30, use formic acid as the solvent, and the mass fraction of the spinning solution is 12%, and add the spinning solution into the syringe of the electrospinning device , adjust the rate of the micro-injection pump to 10 ml / hour, the receiving distance to 12 cm, and the voltage to 20 kV, turn on the electrospinning machine, spin in a cylindrical rotor, and obtain a cell scaffold with an average fiber diameter of 1000-1500 nm layer;

[0111](2) Select hydrophobic polycaprolactone, a mixture of chondroitin sulfate and hyaluronic acid at a mass ratio of 70:30 as the solute, dissolve it in hexafluoroisopropanol to obtain a spinning solution with a mass fraction of 10%, And prepare three different mixed solutions according to the mass ratio of (polycaprolactone: chondroitin sulfate and hyaluronic acid) 25:75, 50:50, 75:25, adjust the speed of the micro-injection pump to 5 ml / hour,...

Embodiment 3

[0115] (1) Prepared as Figure 4 grooved rotor shown;

[0116] (2) Select hydrophilic chondroitin sulfate and hyaluronic acid, the mass ratio of the two is 70:30, the mass fraction of the spinning solution is 12%, add the spinning solution into the syringe of the electrospinning device, and adjust the micro-syringe pump The rate is 10ml / hour, the receiving distance is 12cm, the voltage is 20kV, and spinning is carried out to obtain the inner layer of the cell scaffold with an average fiber diameter of 1000-1500nm; then, water-soluble gelatin gel is coated on the groove;

[0117] (3) Choose hydrophobic L-polylactic acid and ε-caprolactone, the mass ratio of the two is 50:50, it is a copolymerized polymer material, the number average molecular weight is 310000, and the total mass of chondroitin sulfate and hyaluronic acid A mixture with a ratio of 70:30 was used as a solute, dissolved in hexafluoroisopropanol to obtain a spinning solution with a mass fraction of 12%, and accord...

technical field

[0001] The invention belongs to the field of biomedical materials and implantable medical devices, and in particular relates to a nerve guide and a preparation method thereof. This case is a divisional application of the original application "CN201110073822.9 A nerve guide and its preparation method". Background technique

[0002] The nervous system is one of the most important organs in the human body, which controls the sensory and motor functions of the human body. Various traumas such as compression, stretching, tearing, cutting, and other factors such as ischemia, tumors, etc. will cause partial or complete damage to the nervous system, resulting in loss of function and other neurological diseases. The repair and functional reconstruction of defective nerves is one of the difficult problems to be overcome by human beings.

[0003] The neuronal axons of peripheral nerves can extend under appropriate conditions and grow longer than the injured area to ac...

Examples

Embodiment 1

[0105] (1) Select hydrophilic gelatin and silk fibroin, the mass ratio of the two is 70:30, the solvent is formic acid, the mass fraction of the spinning solution is 15%, and the spinning solution is added to the syringe of the electrospinning device, and the micro amount is adjusted The speed of the syringe pump is 8 ml / hour, the receiving distance is 10 cm, and the voltage is 20 kV. Turn on the electrospinning machine and perform spinning to obtain the inner layer of the cell scaffold with an average fiber diameter of 800-1000 nm;

[0106] (2) Select hydrophobic L-polylactic acid and ε-caprolactone copolymerized polymer materials, the mass ratio of the two is 50:50, the number average molecular weight is 310,000, and the mass ratio of gelatin and silk fibroin is 70:30 As a solute, it is dissolved in hexafluoroisopropanol to obtain a spinning solution with a mass fraction of 12%, and based on the mass ratio of (L-polylactic acid and ε-caprolactone: gelatin and silk fibroin) 25...

Embodiment 2

[0110] (1) Select hydrophilic chondroitin sulfate and hyaluronic acid, the mass ratio of the two is 70:30, use formic acid as the solvent, and the mass fraction of the spinning solution is 12%, and add the spinning solution into the syringe of the electrospinning device , adjust the rate of the micro-injection pump to 10 ml / hour, the receiving distance to 12 cm, and the voltage to 20 kV, turn on the electrospinning machine, spin in a cylindrical rotor, and obtain a cell scaffold with an average fiber diameter of 1000-1500 nm layer;

[0111](2) Select hydrophobic polycaprolactone, a mixture of chondroitin sulfate and hyaluronic acid at a mass ratio of 70:30 as the solute, dissolve it in hexafluoroisopropanol to obtain a spinning solution with a mass fraction of 10%, And prepare three different mixed solutions according to the mass ratio of (polycaprolactone: chondroitin sulfate and hyaluronic acid) 25:75, 50:50, 75:25, adjust the speed of the micro-injection pump to 5 ml / hour,...

Embodiment 3

[0115] (1) Prepared as Figure 4 grooved rotor shown;

[0116] (2) Select hydrophilic chondroitin sulfate and hyaluronic acid, the mass ratio of the two is 70:30, the mass fraction of the spinning solution is 12%, add the spinning solution into the syringe of the electrospinning device, and adjust the micro-syringe pump The rate is 10ml / hour, the receiving distance is 12cm, the voltage is 20kV, and spinning is carried out to obtain the inner layer of the cell scaffold with an average fiber diameter of 1000-1500nm; then, water-soluble gelatin gel is coated on the groove;

[0117] (3) Choose hydrophobic L-polylactic acid and ε-caprolactone, the mass ratio of the two is 50:50, it is a copolymerized polymer material, the number average molecular weight is 310000, and the total mass of chondroitin sulfate and hyaluronic acid A mixture with a ratio of 70:30 was used as a solute, dissolved in hexafluoroisopropanol to obtain a spinning solution with a mass fraction of 12%, and accord...

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