Method for tissue culture and rapid propagation of oxalis triangularis

Abstract

The invention discloses a method for tissue culture and rapid propagation of oxalis triangularis. The method comprises the steps of 1, explant selection and sterilization, wherein a blade or petiole of oxalis triangularis is cut off and then sterilized by means of ethyl alcohol and mercury bichloride to obtain an explant; 2, primary culture, wherein a primary culture medium is inoculated with the explant obtained in the step 1 for culture lasting 5-6 weeks till seedlings come out; 3, multiplication culture, wherein a multiplication culture medium is inoculated with the seedlings obtained in the step 2 after the seedlings are cut off for culture lasting 5-6 weeks till multiple shoots come out; 4, rooting culture, wherein the multiple shoots obtained in the step 3 are divided into single plants, and then a rooting culture medium is inoculated with the single plants for culture lasting 2-4 weeks, so that oxalis triangularis aseptic seedlings are obtained; 5, acclimatization and transplantation, wherein the oxalis triangularis aseptic seedlings obtained in the step 4 are transferred to the outside for closed bottle acclimatization lasting 3-5 days and open acclimatization lasting 1-2 days, and then transplantation can be conducted. The adopted culture media are simple in formula, culture time is short, multiplication frequency is high, the variation rate is low, operation is easy, and industrialized production can be realized.

Description

technical field

[0001] The invention relates to the technical field of biological group cultivation of seedlings, in particular to a method for tissue culture and rapid propagation of Oxalis oleracea. Background technique

[0002] Purple leaf sorrel is a perennial herbaceous plant with colorful leaves in the genus Oxalis L. of the Oxalidaceae family (Oxalidaceae), also known as Violet sorrel and purple butterfly. Purple-leaved wood sorrel is produced in the tropical regions of America. It is a rare and excellent ground cover with colorful leaves, and it is more and more favored by flower lovers and gardeners. Purple-leaved wood sorrel has neat plants, purple-red leaves, beautiful and attractive purple-red leaves, and lovely pink flowers. It is an excellent potted plant and ground cover. At present, the propagation speed of conventional seeds of Oxalis oleracea is slow, and the mutation rate is high, the propagation speed of ramets is slow, the labor cost is high, and there ...

Examples

Embodiment 1

[0028] A kind of tissue culture rapid propagation method of Oxalis oleracea described in the present embodiment, comprises the following steps:

[0029] (1) Selection and sterilization of explants: select the leaves or petioles of Oxalis oleifera, wash them with tap water first, then rinse them with running water for 30 minutes, treat them with 75% alcohol for 20-40 seconds, and then use 0.1% Mercury treatment for 3-4 minutes, rinse with sterile water for 4-6 times, absorb water with absorbent paper, cut petioles into 0.5-1.5 cm length, or cut leaves into squares with side length of 0.5-1.5 cm to obtain explants ;

[0030] (2) Primary culture: the explants obtained in step (1) are inoculated into the primary medium with a pH value of 5.8-6.0 and cultivated for 5-6 weeks, and the seedlings are generated. The composition of the primary medium is: MS basic medium , supplemented with 1.0mg / L6-BA, 0.2mg / LNAA, 0.1mg / LIAA, 30g / L sucrose, 5.5g / L agar;

[0031] (3) Proliferation cult...

Embodiment 2

[0037] The tissue culture rapid propagation method of a kind of purple leaf sorrel described in the present embodiment, the difference with embodiment 1 is:

[0038] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 0.5mg / L6-BA, 0.1mg / LNAA, 0.1mg / LIAA, 30g / L sucrose, and 5.5g / L agar;

[0039] In step (3), the composition of the proliferation medium is: MS basic medium supplemented with 0.5mg / L6-BA and 0.2mg / LNAA;

[0040] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 0.1 mg / L NAA, 30 g / L sucrose, and 5.5 g / L agar.

[0041] In the present embodiment, in the primary culture process, the first seedling generation time was 45 days, and when the primary culture ended, the seedlings elongated by 2-3 cm; during the proliferation culture process, the first clustered buds generated time was 23 days, At the end of the multiplication culture, the height of the clustered buds was 5 cm, and the multiplic...

Embodiment 3

[0043] The tissue culture rapid propagation method of a kind of purple leaf sorrel described in the present embodiment, the difference with embodiment 1 is:

[0044] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 1.0mg / L6-BA, 0.3mg / LNAA, 0.2mg / LIAA, 30g / L sucrose, 5.5g / L agar;

[0045] In step (3), the composition of the proliferation medium is: MS basic medium supplemented with 1.0mg / L6-BA and 0.4mg / LNAA;

[0046] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium supplemented with 0.05 mg / L NAA, 20 g / L sucrose, and 5.5 g / L agar.

[0047] In the present embodiment, in the primary culture process, the first seedling generation time was 35 days, and when the primary culture ended, the seedlings elongated by 2-3 cm; during the proliferation culture process, the first clustered buds generated time was 20 days, At the end of the multiplication culture, the height of the clustered buds was 5 cm, and the multiplicat...

technical field

[0001] The invention relates to the technical field of biological group cultivation of seedlings, in particular to a method for tissue culture and rapid propagation of Oxalis oleracea. Background technique

[0002] Purple leaf sorrel is a perennial herbaceous plant with colorful leaves in the genus Oxalis L. of the Oxalidaceae family (Oxalidaceae), also known as Violet sorrel and purple butterfly. Purple-leaved wood sorrel is produced in the tropical regions of America. It is a rare and excellent ground cover with colorful leaves, and it is more and more favored by flower lovers and gardeners. Purple-leaved wood sorrel has neat plants, purple-red leaves, beautiful and attractive purple-red leaves, and lovely pink flowers. It is an excellent potted plant and ground cover. At present, the propagation speed of conventional seeds of Oxalis oleracea is slow, and the mutation rate is high, the propagation speed of ramets is slow, the labor cost is high, and there ...

Examples

Embodiment 1

[0028] A kind of tissue culture rapid propagation method of Oxalis oleracea described in the present embodiment, comprises the following steps:

[0029] (1) Selection and sterilization of explants: select the leaves or petioles of Oxalis oleifera, wash them with tap water first, then rinse them with running water for 30 minutes, treat them with 75% alcohol for 20-40 seconds, and then use 0.1% Mercury treatment for 3-4 minutes, rinse with sterile water for 4-6 times, absorb water with absorbent paper, cut petioles into 0.5-1.5 cm length, or cut leaves into squares with side length of 0.5-1.5 cm to obtain explants ;

[0030] (2) Primary culture: the explants obtained in step (1) are inoculated into the primary medium with a pH value of 5.8-6.0 and cultivated for 5-6 weeks, and the seedlings are generated. The composition of the primary medium is: MS basic medium , supplemented with 1.0mg / L6-BA, 0.2mg / LNAA, 0.1mg / LIAA, 30g / L sucrose, 5.5g / L agar;

[0031] (3) Proliferation cult...

Embodiment 2

[0037] The tissue culture rapid propagation method of a kind of purple leaf sorrel described in the present embodiment, the difference with embodiment 1 is:

[0038] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 0.5mg / L6-BA, 0.1mg / LNAA, 0.1mg / LIAA, 30g / L sucrose, and 5.5g / L agar;

[0039] In step (3), the composition of the proliferation medium is: MS basic medium supplemented with 0.5mg / L6-BA and 0.2mg / LNAA;

[0040] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 0.1 mg / L NAA, 30 g / L sucrose, and 5.5 g / L agar.

[0041] In the present embodiment, in the primary culture process, the first seedling generation time was 45 days, and when the primary culture ended, the seedlings elongated by 2-3 cm; during the proliferation culture process, the first clustered buds generated time was 23 days, At the end of the multiplication culture, the height of the clustered buds was 5 cm, and the multiplic...

Embodiment 3

[0043] The tissue culture rapid propagation method of a kind of purple leaf sorrel described in the present embodiment, the difference with embodiment 1 is:

[0044] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 1.0mg / L6-BA, 0.3mg / LNAA, 0.2mg / LIAA, 30g / L sucrose, 5.5g / L agar;

[0045] In step (3), the composition of the proliferation medium is: MS basic medium supplemented with 1.0mg / L6-BA and 0.4mg / LNAA;

[0046] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium supplemented with 0.05 mg / L NAA, 20 g / L sucrose, and 5.5 g / L agar.

[0047] In the present embodiment, in the primary culture process, the first seedling generation time was 35 days, and when the primary culture ended, the seedlings elongated by 2-3 cm; during the proliferation culture process, the first clustered buds generated time was 20 days, At the end of the multiplication culture, the height of the clustered buds was 5 cm, and the multiplicat...

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