Method for breeding chamomile

Abstract

The invention discloses a method for breeding chamomile. The method disclosed by the invention comprises the following steps: (1) inoculating chamomile seeds onto a seed germination culture medium to carry out sterile seedling cultivation so as to produce sterile seedlings; (2) taking hypocotyls of the sterile seedlings as explants, inoculating the explants onto a callus induction culture medium to carry out callus induction culture so as to generate calluses by virtue of induction; (3) inoculating the calluses generated by induction onto an adventitious bud differentiation culture medium to carry out adventitious bud induction differentiation culture so as to obtain adventitious buds; (4) inoculating the adventitious buds onto a rooting culture medium to carry out rooting culture and culturing adventitious roots by virtue of induction so as to obtain rooted seedlings; and (5) hardening and transplanting the rooted seedlings so as to finally obtain the chamomile. In a regeneration system established by the method disclosed by the invention, callus inductivity reaches up to 86.63%, differentiation rate of the adventitious buds reaches up to 25.5%, rooting rate reaches up to 100%, and transplanting survival rate reaches up to 100%, thus a large number of excellent chamomile test-tube plantlets can be obtained in short term so as to realize large-scale factory production.

Description

technical field

[0001] The invention relates to a plant tissue culture method, in particular to a chamomile in vitro culture and plant regeneration method, and belongs to the field of plant tissue culture. Background technique

[0002] Chamomile (Chrysanthemum lavandulifolium), also known as rock chrysanthemum, chamomile, is a perennial herb of the family Asteraceae and is one of the important parents of the origin of chrysanthemum (Chrysanthemum×morifolium). It has good ornamental value and belongs to wild ornamental plants. It is distributed in many provinces and cities in my country, mostly in hillsides, rocks, river valleys, river banks, wasteland and loess hilly areas at an altitude of 630-2300 meters.

[0003] Chamomile is a good water-saving wild ground cover plant material. It has the advantages of strong stress resistance, wide application range, and extensive management. It is suitable for hillside greening, road slope protection, and river embankment planting, an...

Examples

Embodiment 1

[0052] 1. Test materials

[0053] 1. Chamomile seeds: collected from the campus of Beijing Forestry University in the autumn of 2009, the collected plants grew well, the seeds were mature and plump, and the seed coat was intact.

[0054] 2. Plant growth regulator

[0055] The plant growth regulating substances used in the present invention are domestic naphthaleneacetic acid (NAA), 6-benzylaminoadenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2,4-D).

[0056] 3. Preparation of culture medium:

[0057] (1) The composition or preparation method of "MS basic medium";

[0058] Table 1 MS medium (Murashige and Skoog, 1962)

[0059]

[0060] After the above MS medium mother solution was prepared, it was stored in a refrigerator at 4°C until use. According to the quantity of the configured culture medium, weigh the required agar and sucrose, pour it into sterile water with 3 / 4 of the volume of the culture medium to be prepared, and add the required mother liquor of macroelem...

Embodiment 2

[0092] In addition to the culture time of sterile seedlings is 5 days; 2,4-D 0.5mg / L for callus induction medium, 6-BA 0.1mg / L; NAA 0.2mg / L for rooting medium; adventitious bud differentiation culture light Except that the cycle is 14 hours of light / 10 hours of darkness, the others are the same as in Example 1.

Embodiment 3

[0094] The culture time of sterile seedlings is 6 days; 2,4-D 1.0mg / L and 6-BA 1.0mg / L are used in the callus induction medium; the photoperiod of adventitious bud differentiation culture is 14 hours of light / 10 hours of darkness; Rooting medium used NAA 0.3mg / L, photoperiod is 14 hours of light / 10 hours of darkness, other are all identical with embodiment 1.

technical field

[0001] The invention relates to a plant tissue culture method, in particular to a chamomile in vitro culture and plant regeneration method, and belongs to the field of plant tissue culture. Background technique

[0002] Chamomile (Chrysanthemum lavandulifolium), also known as rock chrysanthemum, chamomile, is a perennial herb of the family Asteraceae and is one of the important parents of the origin of chrysanthemum (Chrysanthemum×morifolium). It has good ornamental value and belongs to wild ornamental plants. It is distributed in many provinces and cities in my country, mostly in hillsides, rocks, river valleys, river banks, wasteland and loess hilly areas at an altitude of 630-2300 meters.

[0003] Chamomile is a good water-saving wild ground cover plant material. It has the advantages of strong stress resistance, wide application range, and extensive management. It is suitable for hillside greening, road slope protection, and river embankment planting, an...

Examples

Embodiment 1

[0052] 1. Test materials

[0053] 1. Chamomile seeds: collected from the campus of Beijing Forestry University in the autumn of 2009, the collected plants grew well, the seeds were mature and plump, and the seed coat was intact.

[0054] 2. Plant growth regulator

[0055] The plant growth regulating substances used in the present invention are domestic naphthaleneacetic acid (NAA), 6-benzylaminoadenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2,4-D).

[0056] 3. Preparation of culture medium:

[0057] (1) The composition or preparation method of "MS basic medium";

[0058] Table 1 MS medium (Murashige and Skoog, 1962)

[0059]

[0060] After the above MS medium mother solution was prepared, it was stored in a refrigerator at 4°C until use. According to the quantity of the configured culture medium, weigh the required agar and sucrose, pour it into sterile water with 3 / 4 of the volume of the culture medium to be prepared, and add the required mother liquor of macroelem...

Embodiment 2

[0092] In addition to the culture time of sterile seedlings is 5 days; 2,4-D 0.5mg / L for callus induction medium, 6-BA 0.1mg / L; NAA 0.2mg / L for rooting medium; adventitious bud differentiation culture light Except that the cycle is 14 hours of light / 10 hours of darkness, the others are the same as in Example 1.

Embodiment 3

[0094] The culture time of sterile seedlings is 6 days; 2,4-D 1.0mg / L and 6-BA 1.0mg / L are used in the callus induction medium; the photoperiod of adventitious bud differentiation culture is 14 hours of light / 10 hours of darkness; Rooting medium used NAA 0.3mg / L, photoperiod is 14 hours of light / 10 hours of darkness, other are all identical with embodiment 1.

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