Method and device for detecting and selecting laser-induce enhanced Raman spectrum in liquid

Abstract

The invention discloses a method and a device for detecting and selecting laser-induce enhanced Raman spectrum in a liquid. The method comprises the following steps of: focusing the inducing light on a suspending to-be-detected sample and forming a three-dimensional light well in liquid; enriching metal nanoparticles on the surface of the to-be-detected sample; and coinciding the focuses of exciting light and inducing light, thereby forming a laser-induce enhanced Raman signal. The realizing method disclosed by the invention is simple and convenient, is obvious in spectrum enhancing effect and is fit for quick detection for in-situ Raman spectrum of a biological sample under a liquid environment; the to-be-detected sample and the metal nanoparticles are hatched together; the method is especially fit for the field of quick detection for diseases, epidemic situation, food safety, and the like; the toxic damage to the biological sample caused by the metal nanoparticles is greatly reduced, so that the true physiological status of living cell can be extremely reflected; the optical tweezers formed according to the method provided by the invention can be used for sorting, extracting and eluting, can realize the function of identifying and sorting Raman spectrum of a single living cell, and is beneficial to the follow-up study on the biological sample.

Description

technical field

[0001] The invention relates to Raman signal sample detection technology, in particular to a detection and sorting method and device for laser-induced enhanced Raman spectrum in liquid. Background technique

[0002] When the light beam is irradiated on the sample, most of the photons of the scattered light are emitted in the form of Rayleigh scattering, and the frequency of the scattered light is equal to that of the incident light. In addition, there are very few photons that will undergo Raman scattering. When these photons interact with the sample, energy exchange will occur, and the frequency of the outgoing light will be blue-shifted or red-shifted, collectively known as Raman shift (RamanShift). Corresponding to anti-Stokes (anti-Stokes) line and Stokes (Stokes) line, respectively. In practical research, a spectrometer is usually used to collect Raman scattered light to form a Raman spectrum. Compared with the most commonly used fluorescence research ...

Examples

Embodiment Construction

[0043] The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.

[0044] Such as figure 1 As shown, the detection and sorting device of laser-induced enhanced Raman spectrum in liquid in this embodiment includes: induced light device 1, excitation light device 2, beam combining mirror 3, first dichroic mirror 4, objective lens 5, sample Pond 6, metal nanoparticle, focusing lens, Raman spectrometer 7, illuminating light device 8 and computer 9; Wherein, hold the sample to be measured dissolved in the liquid in the transparent sample pool 6, metal nanoparticle is injected into the sample pool In the liquid; the induced light device 1 emits a near-infrared laser as the induced light, which is first reflected by the first reflector M1 and then passed through the beam combiner 3, reflected by the first dichroic mirror 4 and entered into the objective lens 5, which is highly focused by the objective lens 5, fr...

technical field

[0001] The invention relates to Raman signal sample detection technology, in particular to a detection and sorting method and device for laser-induced enhanced Raman spectrum in liquid. Background technique

[0002] When the light beam is irradiated on the sample, most of the photons of the scattered light are emitted in the form of Rayleigh scattering, and the frequency of the scattered light is equal to that of the incident light. In addition, there are very few photons that will undergo Raman scattering. When these photons interact with the sample, energy exchange will occur, and the frequency of the outgoing light will be blue-shifted or red-shifted, collectively known as Raman shift (RamanShift). Corresponding to anti-Stokes (anti-Stokes) line and Stokes (Stokes) line, respectively. In practical research, a spectrometer is usually used to collect Raman scattered light to form a Raman spectrum. Compared with the most commonly used fluorescence research ...

Examples

Embodiment Construction

[0043] The present invention will be further elaborated below through specific embodiments in conjunction with the accompanying drawings.

[0044] Such as figure 1 As shown, the detection and sorting device of laser-induced enhanced Raman spectrum in liquid in this embodiment includes: induced light device 1, excitation light device 2, beam combining mirror 3, first dichroic mirror 4, objective lens 5, sample Pond 6, metal nanoparticle, focusing lens, Raman spectrometer 7, illuminating light device 8 and computer 9; Wherein, hold the sample to be measured dissolved in the liquid in the transparent sample pool 6, metal nanoparticle is injected into the sample pool In the liquid; the induced light device 1 emits a near-infrared laser as the induced light, which is first reflected by the first reflector M1 and then passed through the beam combiner 3, reflected by the first dichroic mirror 4 and entered into the objective lens 5, which is highly focused by the objective lens 5, fr...

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