Preparation method of natural plant source food preservative based on roof iris rhizomes

Abstract

The invention discloses a preparation method of natural plant source food preservative based on roof iris rhizomes.The natural plant source food preservative contains roof iris rhizome extract.A preparation method of the roof iris rhizome extract comprises the following steps of 1, mixing dry roof iris rhizomes with radix polygoni multiflori, conducting pulverization, conducting heat reflux extraction with ethanol with the concentration being 65-75%, combining extracting solutions, and concentrating the mixture till no alcohol smell exists, so that a concentrated solution is obtained; 2, sequentially conducting extraction on the concentrated solution obtained in step 1 with petroleum ether, ethyl acetate and water-saturated normal butanol, so that petroleum ether extract, ethyl acetate extract and normal butanol extract are obtained separately; 3, enriching the normal butanol extract with macroporous resin, firstly washing 8-10 column volumes with ethanol with the concentration being 6-10% to remove high-polarity constituents, then eluting 9-13 column volumes with ethanol with the concentration being 65-75%, collecting ethanol eluant with the concentration being 65-75%, and conducting pressure-reduced concentration, wherein radix polygoni multiflori accounts for 5-15% the total weight of roof iris rhizomes and radix polygoni multiflori.

Description

Technical field

[0001] The invention belongs to the field of food, relates to the preparation and application of preservatives, in particular to a preparation method and application of a natural plant-derived preservative based on iris. Background technique

[0002] For a long time, the food industry has been accustomed to using chemical compounds as food preservatives, such as benzoic acid and its salts, sorbic acid and its salts, and parabens, which will inevitably affect the use of The flavor of the food has an adverse effect, and once it exceeds the standard, it will have toxic side effects on the human body. Natural preservatives have the advantages of strong antibacterial properties, safety and non-toxicity, good water solubility, good thermal stability, and certain nutrition and health functions. The development and application of natural plant-derived preservatives and the production of halogen products are in line with natural, safe and nutritious Development direction....

Examples

Embodiment 1

[0017] Example 1: Preparation of natural plant-derived preservative

[0018] (a) Mix and crush 5kg of dried iris and Polygonum multiflorum (10% of total weight), and extract 3 times (25L×3 times) with 70% ethanol under reflux for 2 hours each time. Combine the extracts and concentrate until there is no alcohol smell. Obtain the concentrated solution (3L); (b) Use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) to the concentrated solution of step (a). Extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) The n-butanol extract is enriched with D101 type macroporous resin, and 9 column volumes are washed with 8% ethanol to remove the large poles. For sexual substances, 11 column volumes were eluted with 70% ethanol, and the 70% ethanol eluate was collected and concentrated under reduced pressure.

Embodiment 2

[0019] Example 2: Preparation of natural plant-derived preservative

[0020] (a) Mix and crush 5kg of dried iris and Polygonum multiflorum (5% by weight), and extract 3 times (25L×3 times) with 65% ethanol under hot reflux for 2 hours each time. Combine the extracts and concentrate until there is no alcohol. Obtain the concentrated solution (3L); (b) Use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) to the concentrated solution of step (a). Extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) The n-butanol extract is enriched with D101 type macroporous resin, first rinse 10 column volumes with 6% ethanol to remove the large pole For sexual substances, eluted with 65% ethanol for 13 column volumes, collect the 65% ethanol eluate, and concentrate under reduced pressure.

Embodiment 3

[0021] Example 3: Preparation of natural plant-derived preservative

[0022] (a) Mix and crush 5kg of dried iris and Polygonum multiflorum (15% of total weight), and extract 3 times (25L×3 times) with 75% ethanol under hot reflux for 2 hours each time. Combine the extracts and concentrate until there is no alcohol smell. Obtain the concentrated solution (3L); (b) Use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) to the concentrated solution of step (a). Extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) The n-butanol extract is enriched with D101 type macroporous resin, first rinse with 10% ethanol for 8 column volumes to remove the large pole For sexual substances, eluate with 75% ethanol for 9 column volumes, collect the 75% ethanol eluate, and concentrate under reduced pressure.

Technical field

[0001] The invention belongs to the field of food, relates to the preparation and application of preservatives, in particular to a preparation method and application of a natural plant-derived preservative based on iris. Background technique

[0002] For a long time, the food industry has been accustomed to using chemical compounds as food preservatives, such as benzoic acid and its salts, sorbic acid and its salts, and parabens, which will inevitably affect the use of The flavor of the food has an adverse effect, and once it exceeds the standard, it will have toxic side effects on the human body. Natural preservatives have the advantages of strong antibacterial properties, safety and non-toxicity, good water solubility, good thermal stability, and certain nutrition and health functions. The development and application of natural plant-derived preservatives and the production of halogen products are in line with natural, safe and nutritious Development direction....

Examples

Embodiment 1

[0017] Example 1: Preparation of natural plant-derived preservative

[0018] (a) Mix and crush 5kg of dried iris and Polygonum multiflorum (10% of total weight), and extract 3 times (25L×3 times) with 70% ethanol under reflux for 2 hours each time. Combine the extracts and concentrate until there is no alcohol smell. Obtain the concentrated solution (3L); (b) Use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) to the concentrated solution of step (a). Extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) The n-butanol extract is enriched with D101 type macroporous resin, and 9 column volumes are washed with 8% ethanol to remove the large poles. For sexual substances, 11 column volumes were eluted with 70% ethanol, and the 70% ethanol eluate was collected and concentrated under reduced pressure.

Embodiment 2

[0019] Example 2: Preparation of natural plant-derived preservative

[0020] (a) Mix and crush 5kg of dried iris and Polygonum multiflorum (5% by weight), and extract 3 times (25L×3 times) with 65% ethanol under hot reflux for 2 hours each time. Combine the extracts and concentrate until there is no alcohol. Obtain the concentrated solution (3L); (b) Use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) to the concentrated solution of step (a). Extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) The n-butanol extract is enriched with D101 type macroporous resin, first rinse 10 column volumes with 6% ethanol to remove the large pole For sexual substances, eluted with 65% ethanol for 13 column volumes, collect the 65% ethanol eluate, and concentrate under reduced pressure.

Embodiment 3

[0021] Example 3: Preparation of natural plant-derived preservative

[0022] (a) Mix and crush 5kg of dried iris and Polygonum multiflorum (15% of total weight), and extract 3 times (25L×3 times) with 75% ethanol under hot reflux for 2 hours each time. Combine the extracts and concentrate until there is no alcohol smell. Obtain the concentrated solution (3L); (b) Use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) to the concentrated solution of step (a). Extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) The n-butanol extract is enriched with D101 type macroporous resin, first rinse with 10% ethanol for 8 column volumes to remove the large pole For sexual substances, eluate with 75% ethanol for 9 column volumes, collect the 75% ethanol eluate, and concentrate under reduced pressure.

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