Application of injectable microRNA activated hydrogel to promotion of healing of alveolar bone for implant tooth

Abstract

The invention discloses application of injectable microRNA activated hydrogel to promotion of the healing of alveolar bone for an implant tooth. According to the invention, microRNAs are wrapped by acopolymer of the monomer N-(3-aminopropyl)methacrylamide, the monomer acrylamide and the cross-linking agent ethylene glycol dimethacrylate to form a microRNA nanometer microcapsule; and then the microRNA nanometer microcapsule is mixed with carboxymethyl chitosan and bone meal so as to obtain a composite material. Compared with the prior art, the invention has the advantages that the ossificationquality of alveolar bone can be improved and ossification time can be shortened; the sites of tooth sockets are retained, space is provided for the growth of bone tissue, and the problem of no osteogenesis performance and osteoinductivity of bone meal is overcome; and the healing time of tooth sockets is greatly shortened, and the quality of alveolar bone is improved.

Description

technical field

[0001] The invention belongs to the technical field of biomedical tissue engineering materials, and more specifically relates to a preparation method of microRNA nanometer microcapsules and material compounding and application thereof. Background technique

[0002] At present, oral implant restoration is the best method for repairing dentition defect or loss, but its curative effect is largely limited by local alveolar bone quality and bone mass. Oral implants are performed. Especially for patients with severe chronic periodontitis, and systemic diseases such as osteoporosis and diabetes, if they want to perform oral implants, the problem of alveolar bone tissue regeneration must be solved, and this problem is an urgent clinical need. A big problem to solve. For this reason, many experts and scholars have studied a variety of methods, although some effects have been obtained, but there are still many deficiencies. Currently, the commonly used bone defect r...

Examples

Embodiment 1

[0054] Example 1—preparation of MicroRNA nanocapsules, MicroRNA selects microRNA-21

[0055] Dissolve microRNA-21 at a concentration of 20 μM in 10 μl of RNase-depleted water. Add monomers N-(3-aminopropyl)methacrylamide and acrylamide to deoxygenated RNase-free double-distilled water, and control the molar ratio of the monomer to microRNA-21 to be (the ratio of the amount of substances) 2000: 1. Add the crosslinking agent ethylene glycol dimethacrylate according to the molar ratio of N-(3-aminopropyl)methacrylamide, acrylamide and ethylene glycol dimethacrylate in a ratio of 1:1:1 , put the microRNA-21 solution in a 200 μL microcentrifuge tube and use magnetic stirring to mix evenly. Under the condition of continuous stirring and dispersion (150-200 revolutions per minute), the positively charged monomers are separated by electrostatic interaction and hydrogen bond interaction. , neutral monomers and cross-linkers are enriched around the microRNA-21 molecule. Then add initi...

Embodiment 2

[0060] Example 2—Preparation of MicroRNA nanocapsules-bone meal composite material

[0061] Select the MicroRNA nano-microcapsule solution (dispersion system) prepared in Example 1 and the Bio-oss bone powder with a diameter of 1mm, mix the Bio-oss bone powder and microRNA-21 nano-microcapsule solution at a volume ratio of 50:1, and soak for 2h Take it out for later use.

Embodiment 3

[0062] Embodiment 3—preparation of MicroRNA nano-microcapsules-chitosan composite material

[0063] 300mg carboxymethyl chitosan (80% carboxymethyl degree, number average molecular weight 40000) powder adding 1mL above-mentioned concentration is in the microRNA-21 nanometer microcapsule solution of 5 μ M (the MicroRNA nanometer microcapsule solution prepared in embodiment 1 (dispersion System)), shaking and stirring until the carboxymethyl chitosan is completely dissolved. The obtained mixture was sucked into a syringe, placed in an ice cube for 30 min, then taken out, and placed at 4°C to obtain an injectable microRNA-activated hydrogel material.

technical field

[0001] The invention belongs to the technical field of biomedical tissue engineering materials, and more specifically relates to a preparation method of microRNA nanometer microcapsules and material compounding and application thereof. Background technique

[0002] At present, oral implant restoration is the best method for repairing dentition defect or loss, but its curative effect is largely limited by local alveolar bone quality and bone mass. Oral implants are performed. Especially for patients with severe chronic periodontitis, and systemic diseases such as osteoporosis and diabetes, if they want to perform oral implants, the problem of alveolar bone tissue regeneration must be solved, and this problem is an urgent clinical need. A big problem to solve. For this reason, many experts and scholars have studied a variety of methods, although some effects have been obtained, but there are still many deficiencies. Currently, the commonly used bone defect r...

Examples

Embodiment 1

[0054] Example 1—preparation of MicroRNA nanocapsules, MicroRNA selects microRNA-21

[0055] Dissolve microRNA-21 at a concentration of 20 μM in 10 μl of RNase-depleted water. Add monomers N-(3-aminopropyl)methacrylamide and acrylamide to deoxygenated RNase-free double-distilled water, and control the molar ratio of the monomer to microRNA-21 to be (the ratio of the amount of substances) 2000: 1. Add the crosslinking agent ethylene glycol dimethacrylate according to the molar ratio of N-(3-aminopropyl)methacrylamide, acrylamide and ethylene glycol dimethacrylate in a ratio of 1:1:1 , put the microRNA-21 solution in a 200 μL microcentrifuge tube and use magnetic stirring to mix evenly. Under the condition of continuous stirring and dispersion (150-200 revolutions per minute), the positively charged monomers are separated by electrostatic interaction and hydrogen bond interaction. , neutral monomers and cross-linkers are enriched around the microRNA-21 molecule. Then add initi...

Embodiment 2

[0060] Example 2—Preparation of MicroRNA nanocapsules-bone meal composite material

[0061] Select the MicroRNA nano-microcapsule solution (dispersion system) prepared in Example 1 and the Bio-oss bone powder with a diameter of 1mm, mix the Bio-oss bone powder and microRNA-21 nano-microcapsule solution at a volume ratio of 50:1, and soak for 2h Take it out for later use.

Embodiment 3

[0062] Embodiment 3—preparation of MicroRNA nano-microcapsules-chitosan composite material

[0063] 300mg carboxymethyl chitosan (80% carboxymethyl degree, number average molecular weight 40000) powder adding 1mL above-mentioned concentration is in the microRNA-21 nanometer microcapsule solution of 5 μ M (the MicroRNA nanometer microcapsule solution prepared in embodiment 1 (dispersion System)), shaking and stirring until the carboxymethyl chitosan is completely dissolved. The obtained mixture was sucked into a syringe, placed in an ice cube for 30 min, then taken out, and placed at 4°C to obtain an injectable microRNA-activated hydrogel material.

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