Microbial repair solution and application method thereof in slope protection of sandy foundation pit

Abstract

The invention discloses a microbial repair solution and an application method thereof in slope protection of a sandy foundation pit. The microbial repair solution comprises a microbial mineralizing solution and a microbial cementing solution which are independent of each other. The microbial mineralizing solution consists of a mineralizing bacterial solution and a mineralizing bacteria nutritionalsolution which are independent of each other; and a spraying volume ratio of the mineralizing bacterial solution to the mineralizing bacteria nutritional solution is 1 to (2 to 5). The microbial cementing solution consists of a desert self-sourced cementing bacterial solution and a desert self-sourced cementing bacteria nutritional solution which are independent of each other; and the spraying volume ratio of the desert self-sourced cementing bacterial solution to the desert self-sourced cementing bacteria nutritional solution is 1 to (1 to 3). The pollution-free and environment-friendly microbial repair solution is used for curing a slope of the sandy foundation pit and is a novel foundation pit support way. The traditional microbial curing technology is improved and introduced into thefoundation pit engineering, so that a novel slope protection way is provided for the unprotected open cut excavation of the sandy foundation pit.

Description

technical field

[0001] The present invention relates to a green slope protection method in the technical field of foundation pit excavation protection, in particular to the preparation of a microbial restoration fluid and the application method of the microbial restoration fluid in sandy foundation pit slope protection. Background technique

[0002] With the continuous development of urban construction in our country, the requirements for the development and utilization of urban space, including underground space, are becoming more and more urgent. The development of urban underground engineering has led to more and more foundation pit projects. The construction method is simple, the construction cost is low, and it has been widely used. After the sandy foundation pit is excavated, the side slope of the foundation pit is prone to scour, soil flow and other phenomena under the action of the external environment, and may even cause landslides due to rain erosion. One of the e...

Examples

Embodiment 1

[0046] This embodiment provides a microbial restoration fluid applied to slope protection of sandy foundation pits. The microbial remediation solution includes a microbial mineralization solution and a microbial cementation solution that are independent of each other.

[0047] Wherein, the microbial mineralization solution is composed of mineralizing bacteria liquid and mineralizing bacteria nutrient solution which are independent of each other. The mineralization bacteria liquid includes 1# mineralization bacteria liquid and 2# mineralization bacteria liquid used independently; the spraying amount of the mineralization bacteria liquid is (5~10) L / m 2 , the spraying volume ratio of the mineralizing bacteria liquid and the mineralizing bacteria nutrient solution is 1:(2~5). The mineralizing bacteria nutrient solution includes 3g / L~6g / L NutrientBroth, 0.4mol / L~0.7mol / L CO(NH 2 ) 2 , 0.25mol / L~0.5mol / L CaCl 2 2H 2 O, 0.2mol / L~0.3mol / L NH 4 Cl, 0.15mol / L~0.2mol / L NaHCO 3 . ...

Embodiment 2

[0055] This example provides an application method of microbial restoration fluid in the slope protection of sandy foundation pits, which is applied to sandy foundations with a slope range of (1:0.75) to (1:1.5) in the sandy foundation pit pit.

[0056] refer to image 3 , when the sandy foundation pit is not excavated in layers, the application method includes the following steps:

[0057] Step 1 provides the microbiological remediation fluid applied to the slope protection of sandy foundation pits prepared according to Example 1. Wherein, adopt sprayer to spray described microbial restoration liquid to the side slope surface of described sandy foundation pit. The spray rate of the microbial remediation liquid should ensure that the microbial remediation liquid penetrates into the spray point and does not flow down along the side slope surface of the sandy foundation pit. And before spraying the microbial restoration solution on the sandy foundation pit, first determine th...

Embodiment 3

[0068] In implementation 1, the bacillus cementum bacterial strain that is used to prepare desert self-source cementing bacteria liquid is isolated from desert red loam. Its specific separation method is as follows:

[0069] refer to Figure 9 , the isolation method of bacillus cementum comprises the following steps:

[0070] S1. Take n grams of desert soil at 120-150 cm from the desert soil in Inner Mongolia, put it into 10 n grams of sterile distilled water and shake it for 15-20 minutes, then let it stand for 12 hours, and take the supernatant for subsequent use;

[0071] S2. Inoculate the supernatant into No. 1 liquid medium, and carry out constant temperature shaking culture;

[0072] S3. According to the concentration gradient of dilution, prepare the liquid after constant temperature shaking culture in step S2 into dilution solutions of different concentrations, and apply the dilution solutions of different concentrations on the plates with No. 1 solid medium on the u...

technical field

[0001] The present invention relates to a green slope protection method in the technical field of foundation pit excavation protection, in particular to the preparation of a microbial restoration fluid and the application method of the microbial restoration fluid in sandy foundation pit slope protection. Background technique

[0002] With the continuous development of urban construction in our country, the requirements for the development and utilization of urban space, including underground space, are becoming more and more urgent. The development of urban underground engineering has led to more and more foundation pit projects. The construction method is simple, the construction cost is low, and it has been widely used. After the sandy foundation pit is excavated, the side slope of the foundation pit is prone to scour, soil flow and other phenomena under the action of the external environment, and may even cause landslides due to rain erosion. One of the e...

Examples

Embodiment 1

[0046] This embodiment provides a microbial restoration fluid applied to slope protection of sandy foundation pits. The microbial remediation solution includes a microbial mineralization solution and a microbial cementation solution that are independent of each other.

[0047] Wherein, the microbial mineralization solution is composed of mineralizing bacteria liquid and mineralizing bacteria nutrient solution which are independent of each other. The mineralization bacteria liquid includes 1# mineralization bacteria liquid and 2# mineralization bacteria liquid used independently; the spraying amount of the mineralization bacteria liquid is (5~10) L / m 2 , the spraying volume ratio of the mineralizing bacteria liquid and the mineralizing bacteria nutrient solution is 1:(2~5). The mineralizing bacteria nutrient solution includes 3g / L~6g / L NutrientBroth, 0.4mol / L~0.7mol / L CO(NH 2 ) 2 , 0.25mol / L~0.5mol / L CaCl 2 2H 2 O, 0.2mol / L~0.3mol / L NH 4 Cl, 0.15mol / L~0.2mol / L NaHCO 3 . ...

Embodiment 2

[0055] This example provides an application method of microbial restoration fluid in the slope protection of sandy foundation pits, which is applied to sandy foundations with a slope range of (1:0.75) to (1:1.5) in the sandy foundation pit pit.

[0056] refer to image 3 , when the sandy foundation pit is not excavated in layers, the application method includes the following steps:

[0057] Step 1 provides the microbiological remediation fluid applied to the slope protection of sandy foundation pits prepared according to Example 1. Wherein, adopt sprayer to spray described microbial restoration liquid to the side slope surface of described sandy foundation pit. The spray rate of the microbial remediation liquid should ensure that the microbial remediation liquid penetrates into the spray point and does not flow down along the side slope surface of the sandy foundation pit. And before spraying the microbial restoration solution on the sandy foundation pit, first determine th...

Embodiment 3

[0068] In implementation 1, the bacillus cementum bacterial strain that is used to prepare desert self-source cementing bacteria liquid is isolated from desert red loam. Its specific separation method is as follows:

[0069] refer to Figure 9 , the isolation method of bacillus cementum comprises the following steps:

[0070] S1. Take n grams of desert soil at 120-150 cm from the desert soil in Inner Mongolia, put it into 10 n grams of sterile distilled water and shake it for 15-20 minutes, then let it stand for 12 hours, and take the supernatant for subsequent use;

[0071] S2. Inoculate the supernatant into No. 1 liquid medium, and carry out constant temperature shaking culture;

[0072] S3. According to the concentration gradient of dilution, prepare the liquid after constant temperature shaking culture in step S2 into dilution solutions of different concentrations, and apply the dilution solutions of different concentrations on the plates with No. 1 solid medium on the u...

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