Silver nanowire-mineralized collagen co-assembled bionic stent and production method and application thereof

Abstract

The invention provides a silver nanowire-mineralized collagen co-assembled bionic stent material. The silver nanowire-mineralized collagen co-assembled bionic stent material is a three-dimensional porous stent, and the three-dimensional porous stent is formed by interweaving silver nanowires (AgNW) and collagen fibers covering surfaces of the silver nanowires (AgNW), and further comprises nano-hydroxyapatite with which the collagen fibers are filled. A production method of the silver nanowire-mineralized collagen co-assembled bionic stent material is further provided. In the silver nanowire-mineralized collagen co-assembled bionic stent, collagen molecules covering the surfaces of the silver nanowires can reduce the material biotoxicity, and can avoid effects on the compatibility and the osteogenic property; and when the surface collagen molecules are degraded in a body, part of exposed silver nanowires can effectively inhibit survival of bacteria in an implantation position, so that bone repair / antisepsis dual-mode treatment is achieved.

Description

technical field

[0001] The invention belongs to the field of biomedicine, in particular to a silver nanowire-mineralized collagen co-assembled biomimetic scaffold (IMC / AgNWs) and a preparation method thereof, and also provides the silver nanowire-mineralized collagen co-assembled biomimetic scaffold material in the preparation of treatment and / or prevent the application of infectious bone defect repair drugs. Background technique

[0002] Maxillofacial bone defects caused by maxillofacial infectious diseases such as periodontitis, peri-implantitis, and osteomyelitis of the jaw are a major problem in clinical oral treatment. At present, the use of artificial bone substitute materials is an effective solution for the treatment of bone defects. However, the existing artificial bone materials only have a limited physical support effect, and cannot truly realize the regeneration of bone tissue structure and function, especially the arrangement of collagen and blood vessel form...

Examples

Embodiment 1

[0036] A method for preparing a silver nanowire-mineralized collagen co-assembled biomimetic scaffold material, the specific steps are as follows:

[0037] (1) Preparation of silver nanowires (AgNW): Weigh 0.334g of PVP powder into a three-necked flask, add 17mL of ethylene glycol, dissolve with magnetic stirring and heat to 170°C; weigh 0.025g of silver chloride powder and put In 1mL of ethylene glycol; quickly inject the ethylene glycol solution of silver chloride into the PVP solution at 170°C; after 5 minutes, weigh the previously weighed 0.110g of silver nitrate in ethylene glycol solution, and inject it within 10 minutes In a three-necked flask, continue to react at 170°C for 30 minutes. Continuous magnetic stirring is required during the entire reaction process; Repeat the centrifugation once, then centrifuge at 1000r / min for 15min and repeat three times to obtain a silver nanowire (AgNW) solution; the centrifuged AgNWs are dispersed in water for subsequent characterizati...

Embodiment 2

[0044] The method for preparing a silver nanowire-mineralized collagen co-assembled biomimetic scaffold material; the specific steps are as follows:

[0045] (1) Preparation of silver nanowires (AgNW): Weigh 0.334g of PVP powder into a three-necked flask, add 17mL of ethylene glycol, dissolve with magnetic stirring and heat to 170°C; weigh 0.025g of silver chloride powder and put in 1 mL of ethylene glycol. Quickly inject the ethylene glycol solution of silver chloride into the PVP solution at 170°C; after 5 minutes, weigh the previously weighed 0.110g of silver nitrate in ethylene glycol solution, and inject it into the three-neck flask within 10 minutes, at 170°C Continue the reaction for 30 minutes, and continuous magnetic stirring is required throughout the reaction process; after the reaction is completed, wait for the solution to cool down to room temperature naturally, add appropriate amount of deionized water and ethanol, centrifuge at 8000r / min for 5min and repeat the...

Embodiment 3

[0052] The preparation method of silver nanowire-mineralized collagen co-assembled biomimetic scaffold material, the steps are as follows:

[0053] (1) Preparation of silver nanowires (AgNW): heat the PVP solution, stir at a constant temperature, add silver chloride solution to react; then add silver nitrate solution to react; cool to room temperature, add deionized water and ethanol mixed solution, and centrifuge. Obtain silver nanowire (AgNW) solution;

[0054] (2) Silver nanowire-collagen co-assembly: using thermodynamically controlled bottom-up self-assembly mode, the extracted and purified type I rat tail collagen solution was concentrated with PEG, and the collagen solution was prepared with step (1). The obtained silver nanowire (AgNW) solution was mixed and dialyzed to obtain a co-assembled silver nanowire-collagen;

[0055] (3) Synthesis of a three-dimensional porous scaffold: the co-assembled silver nanowire-collagen obtained in step (2) was centrifuged, stirred int...

technical field

[0001] The invention belongs to the field of biomedicine, in particular to a silver nanowire-mineralized collagen co-assembled biomimetic scaffold (IMC / AgNWs) and a preparation method thereof, and also provides the silver nanowire-mineralized collagen co-assembled biomimetic scaffold material in the preparation of treatment and / or prevent the application of infectious bone defect repair drugs. Background technique

[0002] Maxillofacial bone defects caused by maxillofacial infectious diseases such as periodontitis, peri-implantitis, and osteomyelitis of the jaw are a major problem in clinical oral treatment. At present, the use of artificial bone substitute materials is an effective solution for the treatment of bone defects. However, the existing artificial bone materials only have a limited physical support effect, and cannot truly realize the regeneration of bone tissue structure and function, especially the arrangement of collagen and blood vessel form...

Examples

Embodiment 1

[0036] A method for preparing a silver nanowire-mineralized collagen co-assembled biomimetic scaffold material, the specific steps are as follows:

[0037] (1) Preparation of silver nanowires (AgNW): Weigh 0.334g of PVP powder into a three-necked flask, add 17mL of ethylene glycol, dissolve with magnetic stirring and heat to 170°C; weigh 0.025g of silver chloride powder and put In 1mL of ethylene glycol; quickly inject the ethylene glycol solution of silver chloride into the PVP solution at 170°C; after 5 minutes, weigh the previously weighed 0.110g of silver nitrate in ethylene glycol solution, and inject it within 10 minutes In a three-necked flask, continue to react at 170°C for 30 minutes. Continuous magnetic stirring is required during the entire reaction process; Repeat the centrifugation once, then centrifuge at 1000r / min for 15min and repeat three times to obtain a silver nanowire (AgNW) solution; the centrifuged AgNWs are dispersed in water for subsequent characterizati...

Embodiment 2

[0044] The method for preparing a silver nanowire-mineralized collagen co-assembled biomimetic scaffold material; the specific steps are as follows:

[0045] (1) Preparation of silver nanowires (AgNW): Weigh 0.334g of PVP powder into a three-necked flask, add 17mL of ethylene glycol, dissolve with magnetic stirring and heat to 170°C; weigh 0.025g of silver chloride powder and put in 1 mL of ethylene glycol. Quickly inject the ethylene glycol solution of silver chloride into the PVP solution at 170°C; after 5 minutes, weigh the previously weighed 0.110g of silver nitrate in ethylene glycol solution, and inject it into the three-neck flask within 10 minutes, at 170°C Continue the reaction for 30 minutes, and continuous magnetic stirring is required throughout the reaction process; after the reaction is completed, wait for the solution to cool down to room temperature naturally, add appropriate amount of deionized water and ethanol, centrifuge at 8000r / min for 5min and repeat the...

Embodiment 3

[0052] The preparation method of silver nanowire-mineralized collagen co-assembled biomimetic scaffold material, the steps are as follows:

[0053] (1) Preparation of silver nanowires (AgNW): heat the PVP solution, stir at a constant temperature, add silver chloride solution to react; then add silver nitrate solution to react; cool to room temperature, add deionized water and ethanol mixed solution, and centrifuge. Obtain silver nanowire (AgNW) solution;

[0054] (2) Silver nanowire-collagen co-assembly: using thermodynamically controlled bottom-up self-assembly mode, the extracted and purified type I rat tail collagen solution was concentrated with PEG, and the collagen solution was prepared with step (1). The obtained silver nanowire (AgNW) solution was mixed and dialyzed to obtain a co-assembled silver nanowire-collagen;

[0055] (3) Synthesis of a three-dimensional porous scaffold: the co-assembled silver nanowire-collagen obtained in step (2) was centrifuged, stirred int...

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