A kind of antibacterial medical biological material and preparation method thereof

Abstract

The invention discloses an antibacterial medical biomaterial and a preparation method thereof, wherein the medical biomaterial comprises a decellularized small intestine submucosa matrix material, an antibacterial gel layer located on the surface of the decellularized small intestine submucosa matrix material, and an antibacterial gel layer located on the decellularized small intestine submucosa matrix material. The absorbable fiber layer on the surface of the antibacterial gel layer, silver sulfadiazine is dispersed on the surface of the decellularized small intestine submucosa matrix material and / or in the decellularized small intestine submucosa matrix material; the absorbable fiber layer It includes absorbable fibers attached with silver sulfadiazine, wherein the content of silver sulfadiazine in the absorbable fibers is 1wt.%-2wt.%. The medical biological material provided by the present invention is not only widely used as an external drug for treating wound infection secondary to burns or scalds, but also the traditional central venous catheter can be coated with silver sulfadiazine antibacterial coating to reduce the incidence of infection, so that the load has Medical biomaterials of silver sulfadiazine will also have the same antibacterial activity as silver sulfadiazine.

Description

technical field

[0001] The invention belongs to the technical field of medical biological materials, and in particular relates to an antibacterial medical biological material and a preparation method thereof. Background technique

[0002] Defects and partial or total loss of functions of certain tissues or organs of the body caused by various diseases and traumas are one of the main hazards faced by human health. Research and development of ideal materials for tissue repair has always been an important topic in the fields of medicine, bioscience, and material science. At present, among the many medical biomaterials used in clinic for tissue repair, implantable medical biomaterials are transformed from traditional non-absorbable materials to degradable new biomaterials that can actively induce tissue regeneration. Based on the principle of tissue engineering, extracellular matrix (ECM) materials based on animal tissues are the main development direction.

[0003] In the cli...

Examples

preparation example Construction

[0049] Another aspect of the present invention provides a method for preparing an antibacterial medical biomaterial, comprising:

[0050] a) placing the acellular small intestinal submucosa matrix material in a sodium hydroxide solution containing sulfadiazine, controlling the pH of the solution to be between 7.1 and 13.0, and obtaining an intermediate material coated with sulfadiazine sodium on the surface and / or inside;

[0051] b) After the intermediate material obtained in step a is taken out from the solution, washed with sodium chloride solution, and dried, the intermediate material is soaked in silver nitrate solution, so that the sodium ions on the sodium sulfadiazine molecule are The silver ion substitution in silver nitrate is used to obtain the decellularized small intestinal submucosa matrix material loaded with silver sulfadiazine;

[0052] c) performing surface plasma treatment on the acellular small intestinal submucosa matrix material loaded with silver sulfadi...

Embodiment 1

[0075] A method for preparing an antibacterial medical biomaterial, comprising:

[0076] (1) Immerse the small intestinal submucosa tissue material on the pig body into normal saline for cleaning, put the cleaned small intestinal submucosa tissue material into a closed container, freeze-dry it in liquid nitrogen, seal it, and sterilize it For storage, the freeze-drying temperature is -75°C, and the freeze-drying time is 25 hours. The sterilization is to irradiate the sealed small intestinal submucosa tissue material with a radiation dose of 8000 rads and sterilize it through linear acceleration.

[0077] (2) Treat the small intestinal submucosa tissue material obtained in step a1 with decellularization solution in two ultrasonic frequency environments with different frequencies, and perform decellularization; wherein, the decellularization solution includes trypsin and PBS solution, and the decellularization solution The mass percent concentration of trypsin in the medium was ...

Embodiment 2

[0089] A method for preparing an antibacterial medical biomaterial, comprising:

[0090] (1) Immerse the small intestinal submucosa tissue material on the pig body into normal saline for cleaning, put the cleaned small intestinal submucosa tissue material into a closed container, freeze-dry it in liquid nitrogen, seal it, and sterilize it For storage, the freeze-drying temperature is -78°C, and the freeze-drying time is 25 hours. The sterilization is to sterilize the sealed small intestinal submucosa tissue material through linear acceleration and irradiation with a radiation dose of 8000 rads.

[0091] (2) Treat the small intestinal submucosa tissue material obtained in step a1 with decellularization solution in two ultrasonic frequency environments with different frequencies, and perform decellularization; wherein, the decellularization solution includes trypsin and PBS solution, and the decellularization solution The mass percent concentration of trypsin in the medium is 0....

technical field

[0001] The invention belongs to the technical field of medical biological materials, and in particular relates to an antibacterial medical biological material and a preparation method thereof. Background technique

[0002] Defects and partial or total loss of functions of certain tissues or organs of the body caused by various diseases and traumas are one of the main hazards faced by human health. Research and development of ideal materials for tissue repair has always been an important topic in the fields of medicine, bioscience, and material science. At present, among the many medical biomaterials used in clinic for tissue repair, implantable medical biomaterials are transformed from traditional non-absorbable materials to degradable new biomaterials that can actively induce tissue regeneration. Based on the principle of tissue engineering, extracellular matrix (ECM) materials based on animal tissues are the main development direction.

[0003] In the cli...

Examples

preparation example Construction

[0049] Another aspect of the present invention provides a method for preparing an antibacterial medical biomaterial, comprising:

[0050] a) placing the acellular small intestinal submucosa matrix material in a sodium hydroxide solution containing sulfadiazine, controlling the pH of the solution to be between 7.1 and 13.0, and obtaining an intermediate material coated with sulfadiazine sodium on the surface and / or inside;

[0051] b) After the intermediate material obtained in step a is taken out from the solution, washed with sodium chloride solution, and dried, the intermediate material is soaked in silver nitrate solution, so that the sodium ions on the sodium sulfadiazine molecule are The silver ion substitution in silver nitrate is used to obtain the decellularized small intestinal submucosa matrix material loaded with silver sulfadiazine;

[0052] c) performing surface plasma treatment on the acellular small intestinal submucosa matrix material loaded with silver sulfadi...

Embodiment 1

[0075] A method for preparing an antibacterial medical biomaterial, comprising:

[0076] (1) Immerse the small intestinal submucosa tissue material on the pig body into normal saline for cleaning, put the cleaned small intestinal submucosa tissue material into a closed container, freeze-dry it in liquid nitrogen, seal it, and sterilize it For storage, the freeze-drying temperature is -75°C, and the freeze-drying time is 25 hours. The sterilization is to irradiate the sealed small intestinal submucosa tissue material with a radiation dose of 8000 rads and sterilize it through linear acceleration.

[0077] (2) Treat the small intestinal submucosa tissue material obtained in step a1 with decellularization solution in two ultrasonic frequency environments with different frequencies, and perform decellularization; wherein, the decellularization solution includes trypsin and PBS solution, and the decellularization solution The mass percent concentration of trypsin in the medium was ...

Embodiment 2

[0089] A method for preparing an antibacterial medical biomaterial, comprising:

[0090] (1) Immerse the small intestinal submucosa tissue material on the pig body into normal saline for cleaning, put the cleaned small intestinal submucosa tissue material into a closed container, freeze-dry it in liquid nitrogen, seal it, and sterilize it For storage, the freeze-drying temperature is -78°C, and the freeze-drying time is 25 hours. The sterilization is to sterilize the sealed small intestinal submucosa tissue material through linear acceleration and irradiation with a radiation dose of 8000 rads.

[0091] (2) Treat the small intestinal submucosa tissue material obtained in step a1 with decellularization solution in two ultrasonic frequency environments with different frequencies, and perform decellularization; wherein, the decellularization solution includes trypsin and PBS solution, and the decellularization solution The mass percent concentration of trypsin in the medium is 0....

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