Blue lyocell fabric prepared by dyeing escherichia coli fermentation extract and preparation method of blue lyocell fabric

Abstract

The invention relates to a blue lyocell fabric obtained by dyeing an escherichia coli fermentation extract and a preparation method of the blue lyocell fabric. The method comprises the following steps of culturing escherichia coli by using indole as a substrate, and carrying out purifying to obtain indigo blue pigment; and preparing a dyeing solution from the indigo blue pigment, a phenol oxidase solution, a cellulase solution, an H2O2 solution and the like, putting manganese dioxide, a lyocell fabric and the dyeing solution into a dyeing machine for dyeing, performing enzyme deactivation on the dyed lyocell fabric, and performing washing with tap water and drying to obtain the blue lyocell fabric. The cellulose and the indigo blue pigment are activated by selecting cellulase and polyphenol oxidase respectively, so that the graft polymerization reaction of the cellulose and the indigo blue pigment is realized. The dyeing conditions are mild, and the pollution to the environment is small; and the dyed blue lyocell fabric has a relatively high K / S value, and the soaping fastness grade and the dry rubbing fastness grade are both relatively high.

Description

technical field

[0001] The invention belongs to the technical field of fabric preparation, and in particular relates to a blue lyocell fabric prepared by dyeing an Escherichia coli fermented extract and a preparation method thereof. Background technique

[0002] Humans have been dyeing textile fabrics with natural dyes since ancient times. In the 19th century, a large number of cheap, easy-to-prepare, and wide-ranging synthetic dyes gradually occupied the market. So far, synthetic dyes are still widely used in various industries such as textiles, leather, paper, food, etc. However, some synthetic dyes are banned due to the carcinogenic and sensitizing effects of synthetic precursors or products on the human body. With the improvement of people's living standards, the concept of healthy and environmentally friendly life has begun to be highly respected. Natural dyes are non-toxic, non-carcinogenic, biocompatible, and degradable. They are currently considered to be environme...

Examples

Embodiment 1

[0039] The preparation method of the blue lyocell fabric obtained by dyeing the Escherichia coli fermented extract, the specific method comprises the following steps:

[0040] (1) purification of escherichia coli fermentation extract: described purification method comprises the steps:

[0041] S1: The configuration of the seed medium: glucose 1.1g / L, potassium dihydrogen phosphate 0.6g / L, magnesium sulfate 0.25g / L;

[0042] S2: Configuration of fermentation medium: indole 0.15g / L, agar 1.1g / L, potassium dihydrogen phosphate 1.1g / L, magnesium sulfate 0.25g / L, calcium chloride 0.25g / L;

[0043] S3: Seed cultivation: under sterile conditions, insert Escherichia coli into the seed medium according to the inoculation amount of 1.1%, and cultivate for 54 hours at the temperature of 29°C and the rotation speed of 180r / min;

[0044] S4: Fermentation culture: under sterile conditions, insert the cultivated seed culture solution into the sterilized fermentation medium with an inoculati...

Embodiment 2

[0051] The preparation method of the blue lyocell fabric obtained by dyeing the Escherichia coli fermented extract, the specific method comprises the following steps:

[0052] (1) purification of escherichia coli fermentation extract: described purification method comprises the steps:

[0053] S1: The configuration of the seed medium: glucose 1.0g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L;

[0054] S2: Configuration of fermentation medium: indole 0.1g / L, agar 1.0g / L, potassium dihydrogen phosphate 1.0g / L, magnesium sulfate 0.2g / L, calcium chloride 0.2g / L;

[0055] S3: Seed cultivation: under sterile conditions, insert Escherichia coli into the seed medium according to the inoculum amount of 1%, and cultivate for 48 hours at a temperature of 28°C and a rotational speed of 150r / min;

[0056] S4: Fermentation culture: under sterile conditions, insert the cultivated seed culture solution into the sterilized fermentation medium with an inoculation amount o...

Embodiment 3

[0063] The preparation method of the blue lyocell fabric obtained by dyeing the Escherichia coli fermented extract, the specific method comprises the following steps:

[0064] (1) purification of escherichia coli fermentation extract: described purification method comprises the steps:

[0065] S1: The configuration of the seed medium: glucose 1.2g / L, potassium dihydrogen phosphate 0.7g / L, magnesium sulfate 0.3g / L;

[0066] S2: Configuration of fermentation medium: indole 0.2g / L, agar 1.2g / L, potassium dihydrogen phosphate 1.2g / L, magnesium sulfate 0.3g / L, calcium chloride 0.3g / L;

[0067] S3: Seed cultivation: under sterile conditions, insert Escherichia coli into the seed culture medium according to the inoculation amount of 1.2%, and cultivate for 60 hours at the temperature of 30°C and the rotation speed of 200r / min;

[0068] S4: Fermentation culture: under sterile conditions, insert the cultivated seed culture solution into the sterilized fermentation medium with an inocu...

technical field

[0001] The invention belongs to the technical field of fabric preparation, and in particular relates to a blue lyocell fabric prepared by dyeing an Escherichia coli fermented extract and a preparation method thereof. Background technique

[0002] Humans have been dyeing textile fabrics with natural dyes since ancient times. In the 19th century, a large number of cheap, easy-to-prepare, and wide-ranging synthetic dyes gradually occupied the market. So far, synthetic dyes are still widely used in various industries such as textiles, leather, paper, food, etc. However, some synthetic dyes are banned due to the carcinogenic and sensitizing effects of synthetic precursors or products on the human body. With the improvement of people's living standards, the concept of healthy and environmentally friendly life has begun to be highly respected. Natural dyes are non-toxic, non-carcinogenic, biocompatible, and degradable. They are currently considered to be environme...

Examples

Embodiment 1

[0039] The preparation method of the blue lyocell fabric obtained by dyeing the Escherichia coli fermented extract, the specific method comprises the following steps:

[0040] (1) purification of escherichia coli fermentation extract: described purification method comprises the steps:

[0041] S1: The configuration of the seed medium: glucose 1.1g / L, potassium dihydrogen phosphate 0.6g / L, magnesium sulfate 0.25g / L;

[0042] S2: Configuration of fermentation medium: indole 0.15g / L, agar 1.1g / L, potassium dihydrogen phosphate 1.1g / L, magnesium sulfate 0.25g / L, calcium chloride 0.25g / L;

[0043] S3: Seed cultivation: under sterile conditions, insert Escherichia coli into the seed medium according to the inoculation amount of 1.1%, and cultivate for 54 hours at the temperature of 29°C and the rotation speed of 180r / min;

[0044] S4: Fermentation culture: under sterile conditions, insert the cultivated seed culture solution into the sterilized fermentation medium with an inoculati...

Embodiment 2

[0051] The preparation method of the blue lyocell fabric obtained by dyeing the Escherichia coli fermented extract, the specific method comprises the following steps:

[0052] (1) purification of escherichia coli fermentation extract: described purification method comprises the steps:

[0053] S1: The configuration of the seed medium: glucose 1.0g / L, potassium dihydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L;

[0054] S2: Configuration of fermentation medium: indole 0.1g / L, agar 1.0g / L, potassium dihydrogen phosphate 1.0g / L, magnesium sulfate 0.2g / L, calcium chloride 0.2g / L;

[0055] S3: Seed cultivation: under sterile conditions, insert Escherichia coli into the seed medium according to the inoculum amount of 1%, and cultivate for 48 hours at a temperature of 28°C and a rotational speed of 150r / min;

[0056] S4: Fermentation culture: under sterile conditions, insert the cultivated seed culture solution into the sterilized fermentation medium with an inoculation amount o...

Embodiment 3

[0063] The preparation method of the blue lyocell fabric obtained by dyeing the Escherichia coli fermented extract, the specific method comprises the following steps:

[0064] (1) purification of escherichia coli fermentation extract: described purification method comprises the steps:

[0065] S1: The configuration of the seed medium: glucose 1.2g / L, potassium dihydrogen phosphate 0.7g / L, magnesium sulfate 0.3g / L;

[0066] S2: Configuration of fermentation medium: indole 0.2g / L, agar 1.2g / L, potassium dihydrogen phosphate 1.2g / L, magnesium sulfate 0.3g / L, calcium chloride 0.3g / L;

[0067] S3: Seed cultivation: under sterile conditions, insert Escherichia coli into the seed culture medium according to the inoculation amount of 1.2%, and cultivate for 60 hours at the temperature of 30°C and the rotation speed of 200r / min;

[0068] S4: Fermentation culture: under sterile conditions, insert the cultivated seed culture solution into the sterilized fermentation medium with an inocu...

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