Ultrafiltration tube and use method thereof

Abstract

The invention discloses a laboratory separation device technology, and aims to provide an ultrafiltration tube which is characterized by comprising a centrifugal tube, a filtering device inserted into the centrifugal tube and a cover body which is in threaded connection with the centrifugal tube and is used for fixing the filtering device into the centrifugal tube, and the filtering device comprises a containing tube; square grooves communicated with the interior of the containing pipe are symmetrically formed in the bottom of the containing pipe, ultrafiltration membranes covering the square grooves are fixedly arranged on the inner sides of the square grooves, a filter plate covering the outer sides of the square grooves is further arranged on the containing pipe, and a plurality of filter assemblies used for discharging waste liquid filtered by the ultrafiltration membranes into the centrifugal pipe are arranged at the bottom of the filter plate; a sealing leakage-proof structure is arranged in the accommodating pipe and close to the opening; according to the invention, the sealing performance of the ultrafiltration tube is good; by arranging the filtering assembly, the through hole in the filtering device can be sealed and protected, dust is prevented from entering and blocking the through hole, and the working efficiency is prevented from being influenced.

Description

Technical Field

[0001] The present invention relates to a laboratory separation device technology, and more specifically, to an ultrafiltration tube and a method for using the same. Background Art

[0002] Ultrafiltration tube is a membrane filtration device driven by centrifugal force, which is used to separate different molecules or particles in liquid. It is mainly composed of a cover, a filter device, a centrifuge tube and other parts, among which the filter device can be used to permeate or retain samples. Ultrafiltration tube uses a special ultrafiltration membrane to push the liquid sample through the membrane by applying pressure, thereby separating the sizes of different molecules or particles.

[0003] The existing ultrafiltration tubes still have some defects when in use: the existing ultrafiltration tubes are usually placed in a centrifuge when in use to ultrafilter the solution in the ultrafiltration tube by centrifugal force. In this process, the solution in the ultrafiltration tube may leak out due to insufficient sealing, affecting work efficiency; in addition, the ultrafiltration tube is often not ultrafiltered once, but needs to be ultrafiltered multiple times. After each ultrafiltration, the filter device needs to be taken out of the centrifuge tube, the waste liquid in the centrifuge tube is poured out, and then the filter device is put back into the centrifuge tube and the replacement buffer is added again for ultrafiltration. When too many ultrafiltration times are required, dust may clog the through holes on the outside of the filter device when the filter device is taken out, affecting work efficiency. Summary of the invention

[0004] In view of the shortcomings of the prior art, the object of the present invention is to provide an ultrafiltration tube with good sealing and anti-clogging performance and a method for using the same.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: an ultrafiltration tube, comprising a centrifuge tube, a filter device inserted in the centrifuge tube, and a cover body threadedly connected to the centrifuge tube for fixing the filter device in the centrifuge tube, the filter device comprising a receiving tube, a square groove connected to the interior of the receiving tube is symmetrically opened at the bottom of the receiving tube, an ultrafiltration membrane covering the square groove is fixedly arranged on the inner side of the square groove, a filter plate covering the outer side of the square groove is also arranged on the receiving tube, and a plurality of filter components for discharging waste liquid filtered by the ultrafiltration membrane into the centrifuge tube are arranged at the bottom of the filter plate; a sealing and leak-proof structure is arranged near the opening in the receiving tube.

[0006] The present invention is further configured as follows: the filter assembly is arranged in a straight line as a whole, the filter assembly includes a through hole opened on the filter plate, a drainage surface is provided on the outer side of the filter plate and is located on the upper side of the through hole and gradually inclined toward the outside, and an elastic arc surface is provided on the outer side of the filter plate and is located on the lower side of the through hole and gradually inclined toward the outside, the length of the elastic arc surface is greater than the length of the drainage surface, and the elastic arc surface and the drainage surface can completely seal the through hole in a static state.

[0007] The present invention is further configured as follows: a filter screen covering the through hole is arranged on the inner side of the through hole.

[0008] The present invention is further configured as follows: the sealing and leak-proof structure is configured as a pipe plug, a step surface one is configured inside the accommodating tube near the opening, a step surface two is configured in the middle of the pipe plug, an annular elastic waterproof membrane fixedly sleeved on the pipe plug is configured between the step surface one and the step surface two, an annular arc groove is configured at the corner of the step surface one, and a sealing ring is configured in the annular arc groove.

[0009] The present invention is further configured as follows: an annular square groove for fixing the sealing ring is also opened in the middle part of the annular arc groove, and an annular connector corresponding to and adapted to the annular square groove is provided on the sealing ring. The sealing ring is fixed in the annular arc groove by pressing the annular connector into the annular square groove in the annular arc groove.

[0010] The present invention is further configured as follows: a reinforcement ring is also provided inside the sealing ring, the reinforcement ring is arranged coaxially with the sealing ring, the inner wall surface of the sealing ring is provided with a plurality of inwardly concave arc surfaces directly in contact with the reinforcement ring, the plurality of arc surfaces are evenly distributed in the circumferential direction and the length direction of the sealing ring, and the plurality of arc surfaces are arranged around the reinforcement ring.

[0011] The present invention is further configured as follows: the curvature radii of some or all of the plurality of curved surfaces are different; and the plurality of curved surfaces are distributed in an array.

[0012] The present invention is further configured such that the total area of ​​the plurality of arc surfaces accounts for 80%-100% of the inner wall surface of the sealing ring.

[0013] The present invention is further configured as follows: the top surface of the tube plug is higher than the top surface of the receiving tube, and the ratio of the overall thickness of the tube plug to the distance between the step hole 1 and the top surface of the receiving tube is 1-1.5.

[0014] A method for using an ultrafiltration tube, characterized in that it comprises the following steps:

[0015] S1. Place the filter device in a centrifuge tube, then add the solution to be ultrafiltered into the filter device, then press the annular connector on the sealing ring into the annular square groove so that the sealing ring is fixed in the annular square groove, then put the pipe plug into the top of the filter device, and finally tighten the cover body and the centrifuge tube;

[0016] S2. Place the fixed ultrafiltration tube into a suitable centrifuge, adjust various parameters and perform ultrafiltration. The elastic arc surface in the filter device will open due to the centrifugal force of the centrifuge, so that the waste liquid after ultrafiltration flows into the centrifuge tube;

[0017] S3, after the ultrafiltration is completed, the ultrafiltration tube is taken out from the centrifuge, and then the cover is unscrewed, the tube plug is taken out, and then the filter device is taken out, and then the waste liquid in the centrifuge tube is poured to the designated place and the filter device is put back into the centrifuge tube, and then the replacement buffer is added to the filter device. After the addition is completed, the tube plug is placed in the top of the filter device, and finally the cover and the centrifuge tube are tightened and fixed again, and then go to S2, and ultrafiltration is performed again, and the ultrafiltration number is set to A;

[0018] S4. After ultrafiltration A times, take out the ultrafiltration tube from the centrifuge, unscrew the cover, take out the tube plug, and then take out the filter device. Then pour the waste liquid in the centrifuge tube to the designated place, put the filter device back into the centrifuge tube, tighten the cover and the centrifuge tube, and put it in the storage place.

[0019] The beneficial effects of the present invention are:

[0020] 1. Compared with the prior art, the ultrafiltration tube of the present invention is provided with a sealing and leak-proof structure. After the filter device is placed in the centrifuge tube, the tube plug is placed in the receiving tube, and finally the cover body is tightened with the centrifuge tube, so that the ultrafiltration tube has good sealing performance, avoiding the phenomenon of seepage in the ultrafiltration tube during the ultrafiltration process of the centrifuge, and improving the work efficiency; and by providing a filter assembly, when the filter device needs to be taken out for multiple ultrafiltrations, the through holes on the filter device can be sealed and protected to prevent dust from entering and clogging the through holes and affecting the work efficiency.

[0021] 2. During the use of the ultrafiltration tube of the present invention, the filter device is first placed in the centrifuge tube, and then the solution to be ultrafiltered is added to the filter device, and then the sealing ring is fixed in the annular arc groove, and then the pipe plug is placed in the receiving tube of the filter device. Due to the effects of the step surface 1, the step surface 2, and the annular elastic waterproof membrane, the pipe plug will be stuck in the receiving tube, and finally the cover body is rotated and fixedly connected to the centrifuge tube, and the cover body will press the pipe plug into the receiving tube through the annular elastic waterproof membrane, thereby playing a sealing and waterproof role. Secondly, the role of the sealing ring is to further improve the sealing and waterproof effect to prevent the solution from seeping out. After sealing, the ultrafiltration tube is placed in a centrifuge, and the solution is ultrafiltered by high-speed centrifugal force. When the ultrafiltration tube is placed in the centrifuge for ultrafiltration, the high-speed rotation will cause the elastic arc surface in the filter assembly to open due to inertia, so that the filtered waste liquid enters the centrifuge tube, and when the centrifuge stops, the elastic arc surface will automatically return to its original position due to elasticity and cooperate with the drainage surface to seal the through hole, thereby preventing dust from clogging the through hole and affecting the processing efficiency when the filter device is taken out after multiple ultrafiltrations.

[0022] 3. In the present invention, the top surface of the tube plug is higher than the top surface of the receiving tube, which is convenient for fixing the tube plug in the receiving tube when the cover body is fixedly connected to the centrifuge tube. The ratio of the overall thickness of the tube plug to the distance from the step hole 1 to the top surface of the receiving tube is 1-1.5, which plays a sealing role. When the ratio is less than 1, the overall thickness of the tube plug is too small, which may cause the tube plug to move in the receiving tube when the cover body is fixed to the centrifuge tube, affecting the sealing; when the ratio is greater than 1.5, the overall thickness of the tube plug is too large, which may not only cause incomplete fixation of the cover body and the centrifuge tube, but also reduce the volume in the receiving tube, affecting the solution holding space. Therefore, it is preferred that the ratio of the overall thickness of the tube plug to the distance from the step hole 1 to the top surface of the receiving tube is 1-1.5.

[0023] 4. The present invention has a simple and reasonable structure, is easy to manufacture, and is easy to operate. It avoids the defects of the prior art and is suitable for popularization and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a structural diagram of the ultrafiltration tube of the present invention.

[0025] Figure 2 It is an exploded view of the ultrafiltration tube of the present invention.

[0026] Figure 3 It is a structural diagram of the ultrafiltration tube sealing and leak-proof structure of the present invention.

[0027] Figure 4 Ultrafiltration tube of the present invention Figure 3 Enlarged image of .

[0028] Figure 5 It is a partial structural diagram of the ultrafiltration tube filtering device of the present invention when it is stationary.

[0029] Figure 6 Ultrafiltration tube of the present invention Figure 5 Enlarged image of .

[0030] Figure 7 It is a partial structural diagram of the ultrafiltration tube filtering device of the present invention during the ultrafiltration process.

[0031] Figure 8 Ultrafiltration tube of the present invention Figure 7 Enlarged image of .

[0032] Fig. 9 It is a cross-sectional structural diagram of the ultrafiltration tube sealing ring of the present invention.

[0033] Figure 1-9Figure numerals: 1. centrifuge tube; 2. filter device; 3. cover body; 4. accommodating tube; 5. square groove; 6. ultrafiltration membrane; 7. filter plate; 8. filter assembly; 9. through hole; 10. drainage surface; 11. elastic arc surface; 12. filter net; 13. pipe plug; 14. step surface one; 15. step surface two; 16. annular elastic waterproof membrane; 17. annular circular arc groove; 18. sealing ring; 19. annular square groove; 20. annular connector; 21. reinforcement ring; 22. arc surface. DETAILED DESCRIPTION

[0034] Reference Figure 1-9 The ultrafiltration tube of the present invention and the use method thereof are further described in the following embodiments.

[0035] For ease of explanation, spatial relative terms such as "upper", "lower", "left", "right" and the like are used in the embodiments to illustrate the relationship of one element or feature shown in the figure relative to another element or feature. It should be understood that, in addition to the orientation shown in the figure, the spatial terms are intended to include different orientations of the device in use or operation. For example, if the device in the figure is inverted, the element described as being "under" other elements or features will be positioned "on" other elements or features. Therefore, the exemplary term "under" can include both upper and lower orientations. The device can be positioned in other ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used here can be interpreted accordingly.

[0036] Furthermore, relational terms such as “first” and “second” and the like are merely used to distinguish one component from another having the same name, but do not necessarily require or imply any such actual relationship or order between these components.

[0037] Figures 1 to 9The ultrafiltration tube shown in the figure comprises a centrifuge tube 1, a filter device 2 inserted in the centrifuge tube 1, and a cover body 3 threadedly connected to the centrifuge tube 1 for fixing the filter device 2 in the centrifuge tube 1, the filter device 2 comprises a receiving tube 4, a square groove 5 symmetrically opened at the bottom of the receiving tube 4 for communicating with the inside of the receiving tube 4, an ultrafiltration membrane 6 covering the square groove 5 is fixedly arranged inside the square groove 5, a filter plate 7 covering the outside of the square groove 5 is also arranged on the receiving tube 4, and a plurality of filter components 8 for discharging waste liquid filtered by the ultrafiltration membrane 6 into the centrifuge tube 1 are arranged at the bottom of the filter plate 7; the receiving tube 4 is close to the inner surface of the receiving tube 4. A sealing and leak-proof structure is provided at the opening; compared with the prior art, the ultrafiltration tube of the present invention is provided with a sealing and leak-proof structure. After the filter device 2 is placed in the centrifuge tube 1, the tube plug 13 is placed in the receiving tube 4, and finally the cover body 3 is tightened with the centrifuge tube 1, so that the sealing performance of the ultrafiltration tube is good, and the phenomenon of seepage of the ultrafiltration tube during the ultrafiltration process of the centrifuge is avoided, thereby improving the work efficiency; and by providing the filter assembly 8, when the filter device 2 needs to be taken out for multiple ultrafiltrations, the through hole 9 on the filter device 2 can be sealed and protected to prevent dust from entering and clogging the through hole 9 and affecting the work efficiency.

[0038] The filter assembly 8 is arranged in a straight line as a whole, and the filter assembly 8 includes a through hole 9 opened on the filter plate 7, and a drainage surface 10 is provided on the outer side of the filter plate 7 and is located on the upper side of the through hole 9 and gradually tilted toward the outside. The outer side of the filter plate 7 is provided with an elastic arc surface 2211 located on the lower side of the through hole 9 and gradually tilted toward the outside. The length of the elastic arc surface 2211 is greater than the length of the drainage surface 10, and the elastic arc surface 2211 and the drainage surface 10 can completely seal the through hole 9 in a static state; a filter screen 12 covering the through hole 9 is provided on the inner side of the through hole 9 to further prevent dust from entering;

[0039] The sealing and leak-proof structure is provided as a pipe plug 13, a step surface 14 is provided inside the accommodating pipe 4 near the opening, a step surface 2 15 is provided in the middle of the pipe plug 13, an annular elastic waterproof membrane 16 fixedly sleeved on the pipe plug 13 is provided between the step surface 14 and the step surface 2 15, an annular arc groove 17 is provided at the corner of the step surface 14, and a sealing ring 18 is provided in the annular arc groove 17;

[0040] The annular arc groove 17 is provided with an annular square groove 19 in the middle thereof for fixing the sealing ring 18. The sealing ring 18 is provided with an annular connector 20 corresponding to and matching the annular square groove 19. The sealing ring 18 is fixed in the annular arc groove 17 by pressing the annular connector 20 into the annular square groove 19 in the annular arc groove 17.

[0041] During the use of the ultrafiltration tube of the present invention, the filter device 2 is first placed in the centrifuge tube 1, and then the solution to be ultrafiltered is added to the filter device 2, and then the sealing ring 18 is fixed in the annular arc groove 17, and then the pipe plug 13 is placed in the receiving tube 4 of the filter device 2. Due to the effects of the step surface 14, the step surface 2 15, and the annular elastic waterproof membrane 16, the pipe plug 13 will be stuck in the receiving tube 4, and finally the cover body 3 is rotated and fixedly connected with the centrifuge tube 1, and the cover body 3 will press the pipe plug 13 into the receiving tube 4 through the annular elastic waterproof membrane 16, so as to play a sealing and waterproof role. Secondly, the role of the sealing ring 18 is to further improve the sealing and waterproof effect to prevent the solution from leaking. After sealing, the ultrafiltration tube is placed in the centrifuge to ultrafilter the solution through high-speed centrifugal force. When the ultrafiltration tube is placed in the centrifuge for ultrafiltration, the high-speed rotation will cause the elastic arc surface 2211 in the filter assembly 8 to open due to inertia, allowing the filtered waste liquid to enter the centrifuge tube 1. When the centrifuge stops, the elastic arc surface 2211 will automatically return to its original position due to elasticity and cooperate with the drainage surface 10 to seal the through hole 9, preventing dust from clogging the through hole 9 and affecting the processing efficiency when the filter device 2 is taken out after multiple ultrafiltrations.

[0042] A reinforcing ring 21 is also provided in the sealing ring 18, and the reinforcing ring 21 is arranged coaxially with the sealing ring 18. The inner wall surface of the sealing ring 18 is provided with a plurality of inwardly concave arc surfaces 22 directly in contact with the reinforcing ring 21, and the plurality of arc surfaces 22 are evenly arranged in the circumferential direction and the length direction of the sealing ring 18, and the plurality of arc surfaces 22 are arranged around the reinforcing ring 21; the curvature radii of some or all of the plurality of arc surfaces 22 are different; the plurality of arc surfaces 22 are distributed in an array; the total area of ​​the plurality of arc surfaces 22 accounts for 80%-100% of the inner wall surface of the sealing ring 18; and the reinforcing ring 21 is provided in the sealing ring 18 to further improve the sealing performance and prevent the solution from leaking out.

[0043] The top surface of the tube plug 13 is higher than the top surface of the receiving tube 4, and the ratio of the overall thickness of the tube plug 13 to the distance from the step hole 1 to the top surface of the receiving tube 4 is 1-1.5; in the present invention, the top surface of the tube plug 13 is higher than the top surface of the receiving tube 4, which is convenient for fixing the tube plug 13 in the receiving tube 4 when the cover body 3 is fixedly connected to the centrifuge tube 1, and the ratio of the overall thickness of the tube plug 13 to the distance from the step hole 1 to the top surface of the receiving tube 4 is 1-1.5, which plays a sealing role. When the ratio is less than 1, the overall thickness of the tube plug 13 is too small, which may cause the tube plug 13 to move in the receiving tube 4 when the cover body 3 is fixed to the centrifuge tube 1, affecting the sealing performance; when the ratio is greater than 1.5, the overall thickness of the tube plug 13 is too large, which may not only cause the cover body 3 to be incompletely fixed to the centrifuge tube 1, but also reduce the volume in the receiving tube 4, affecting the accommodation space of the solution. Therefore, it is preferred that the ratio of the overall thickness of the tube plug 13 to the distance from the step hole 1 to the top surface of the receiving tube 4 is 1-1.5.

[0044] A method for using an ultrafiltration tube, characterized in that it comprises the following steps:

[0045] S1, place the filter device 2 in the centrifuge tube 1, then add the solution to be ultrafiltered into the filter device 2, then press the annular connector 20 on the sealing ring 18 into the annular square groove 19, so that the sealing ring 18 is fixed in the annular square groove 19, and then put the pipe plug 13 into the top of the filter device 2, and finally tighten the cover 3 and the centrifuge tube 1 to fix it;

[0046] S2, placing the fixed ultrafiltration tube into a suitable centrifuge, adjusting various parameters and performing ultrafiltration, the elastic arc surface 11 in the filter device 2 will open due to the centrifugal force of the centrifuge, so that the waste liquid after ultrafiltration of the solution flows into the centrifuge tube 1;

[0047] S3, after the ultrafiltration is finished, the ultrafiltration tube is taken out from the centrifuge, and then the cover body 3 is unscrewed, the tube plug 13 is taken out, and then the filter device 2 is taken out, and then the waste liquid in the centrifuge tube 1 is poured to the designated place and the filter device 2 is put back into the centrifuge tube 1, and then the replacement buffer is added to the filter device 2. After the addition is completed, the tube plug 13 is put into the top of the filter device 2, and finally the cover body 3 and the centrifuge tube 1 are tightened and fixed again, and then go to S2, and ultrafiltration is performed again, and the ultrafiltration number is set to A;

[0048] S4. After ultrafiltration A times, take out the ultrafiltration tube from the centrifuge, unscrew the cover 3, take out the tube plug 13, and then take out the filter device 2. Then pour the waste liquid in the centrifuge tube 1 to the designated place, put the filter device 2 back into the centrifuge tube 1, tighten the cover 3 and the centrifuge tube 1, and put it in the storage place.

[0049] The invention has a simple and reasonable structure, is easy to manufacture, and is simple to operate, and avoids the defects in the prior art, and is suitable for popularization and implementation.

[0050] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Common changes and substitutions made by those skilled in the art within the scope of the technical solution of the present invention should be included in the protection scope of the present invention.

Claims

1. An ultrafiltration tube, comprising a centrifuge tube (1), a filter device (2) inserted in the centrifuge tube (1), and a cover (3) threadedly connected to the centrifuge tube (1) for fixing the filter device (2) in the centrifuge tube (1), characterized in that: The filtering device (2) comprises a containing tube (4), the bottom of which is symmetrically provided with a square groove (5) communicating with the inside of the containing tube (4), an ultrafiltration membrane (6) covering the square groove (5) being fixedly arranged inside the square groove (5), a filter plate (7) covering the outside of the square groove (5) being also arranged on the containing tube (4), and a plurality of filter components (8) for discharging waste liquid filtered by the ultrafiltration membrane (6) into the centrifuge tube (1) being arranged at the bottom of the filter plate (7); a sealing and leakproof structure is arranged near the opening inside the containing tube (4).

2. An ultrafiltration tube according to claim 1, characterized in that: The filter assembly (8) is arranged in a straight line as a whole. The filter assembly (8) comprises a through hole (9) formed on a filter plate (7). A drainage surface (10) is provided on the outside of the filter plate (7) and is located on the upper side of the through hole (9) and gradually tilted toward the outside. An elastic arc surface (22) (11) is provided on the outside of the filter plate (7) and is located on the lower side of the through hole (9) and gradually tilted toward the outside. The length of the elastic arc surface (22) (11) is greater than the length of the drainage surface (10), and the elastic arc surface (22) (11) and the drainage surface (10) can completely seal the through hole (9) in a static state.

3. An ultrafiltration tube according to claim 2, characterized in that: A filter screen (12) covering the through hole (9) is arranged on the inner side of the through hole (9).

4. An ultrafiltration tube according to claim 1, characterized in that: The sealing and leak-proof structure is provided as a pipe plug (13), wherein a step surface 1 (14) is provided inside the accommodating pipe (4) near the opening, a step surface 2 (15) is provided in the middle of the pipe plug (13), an annular elastic waterproof membrane (16) fixedly sleeved on the pipe plug (13) is provided between the step surface 1 (14) and the step surface 2 (15), an annular circular arc groove (17) is provided at the corner of the step surface 1 (14), and a sealing ring (18) is provided in the annular circular arc groove (17).

5. An ultrafiltration tube according to claim 4, characterized in that: An annular square groove (19) for fixing the sealing ring (18) is also provided in the middle of the annular circular arc groove (17). An annular connector (20) corresponding to and matching the annular square groove (19) is provided on the sealing ring (18). The sealing ring (18) is fixed in the annular circular arc groove (17) by pressing the annular connector (20) into the annular square groove (19) in the annular circular arc groove (17).

6. An ultrafiltration tube according to claim 5, characterized in that: A reinforcing ring (21) is also provided inside the sealing ring (18). The reinforcing ring (21) is coaxially arranged with the sealing ring (18). The inner wall surface of the sealing ring (18) is provided with a plurality of inwardly concave arc surfaces (22) that are directly in contact with the reinforcing ring (21). The plurality of arc surfaces (22) are evenly arranged in the circumferential direction and the length direction of the sealing ring (18), and the plurality of arc surfaces (22) are arranged around the reinforcing ring (21).

7. An ultrafiltration tube according to claim 6, characterized in that: The curvature radii of some or all of the plurality of curved surfaces (22) are different; and the plurality of curved surfaces (22) are distributed in an array.

8. An ultrafiltration tube according to claim 7, characterized in that: The total area of ​​the plurality of arc surfaces (22) accounts for 80%-100% of the inner wall surface of the sealing ring (18).

9. An ultrafiltration tube according to claim 4, characterized in that: The top surface of the tube plug (13) is higher than the top surface of the receiving tube (4), and the ratio of the overall thickness of the tube plug (13) to the distance between the step hole 1 and the top surface of the receiving tube (4) is 1-1.

5.

10. A method for using the ultrafiltration tube according to claims 1 to 9, characterized in that: The following steps are involved: S1. Place the filter device (2) in the centrifuge tube (1), then add the solution to be ultrafiltered into the filter device (2), then press the annular connector (20) on the sealing ring (18) into the annular square groove (19), so that the sealing ring (18) is fixed in the annular square groove (19), then put the pipe plug (13) into the top of the filter device (2), and finally tighten the cover (3) and the centrifuge tube (1) to fix them; S2, placing the fixed ultrafiltration tube into a suitable centrifuge, adjusting various parameters and performing ultrafiltration, the elastic arc surface (11) in the filter device (2) will open due to the centrifugal force of the centrifuge, so that the waste liquid after the ultrafiltration of the solution flows into the centrifuge tube (1); S3, after the ultrafiltration is completed, the ultrafiltration tube is taken out from the centrifuge, and then the cover (3) is unscrewed, the tube plug (13) is taken out, and then the filter device (2) is taken out, and then the waste liquid in the centrifuge tube (1) is poured into the designated place and the filter device (2) is put back into the centrifuge tube (1), and then the replacement buffer is added to the filter device (2). After the addition is completed, the tube plug (13) is placed in the top of the filter device (2), and finally the cover (3) and the centrifuge tube (1) are tightened and fixed again, and then go to S2, and ultrafiltration is performed again, and the ultrafiltration number is set to A; S4. After ultrafiltration A times, take the ultrafiltration tube out of the centrifuge, unscrew the cover (3), take out the tube plug (13), and then take out the filter device (2). Then pour the waste liquid in the centrifuge tube (1) to a designated place, put the filter device (2) back into the centrifuge tube (1), tighten the cover (3) and the centrifuge tube (1), and put it in a storage place.

Images