Active peptide composition for improving male sperm motility and preparation method thereof

Abstract

The invention belongs to the technical field of biological medicine, and particularly discloses an active peptide composition for improving male sperm motility and a preparation method thereof, the composition comprises the following raw material components in parts by weight: 15-18 parts of oyster peptide, 10-28 parts of sea cucumber peptide, 6-10 parts of cordyceps militaris peptide, 10-12 parts of fish white peptide, 15-25 parts of glutathione, 10-20 parts of wheat peptide, and 0.2-0.4 part of coenzyme Q10. According to the active peptide composition for improving the male sperm motility and the preparation method, through the synergistic effect of the specific active peptide composition, the male sperm motility is effectively improved, and the active peptide composition is natural, safe and free of side effects.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to an active peptide composition for improving male sperm motility and a preparation method thereof. Background Art

[0002] Active peptides, also known as bioactive peptides or bioactive polypeptides, are a class of compounds formed by two or more amino acids connected by peptide bonds. These compounds play an important physiological role in the human body and have a key impact on human growth and development, metabolism, disease, aging and death. The changes in the secretion of active peptides in the human body determine the different stages of the human life cycle, such as infancy, childhood, adulthood and old age, until death.

[0003] The application range of active peptides is very wide, covering many fields such as health products, medicine, and cosmetics. In health products, active peptides are often used to enhance immunity, improve intestinal function, reduce blood lipids, and regulate blood sugar; in the field of medicine, active peptides are used to treat diseases such as cancer and cardiovascular disease; in the field of cosmetics, active peptides are used for whitening, anti-wrinkle, and promoting skin repair. In addition, due to its high physiological activity and biocompatibility, active peptides are also used to prevent or treat various diseases.

[0004] Active peptides have a simple structure and a small molecular weight. They can be quickly absorbed through the small intestinal mucosa without the need for re-digestion or energy consumption, and have the characteristics of 100% absorption. The absorption, conversion and utilization of small molecule active peptides are efficient and complete. The fact that small molecule active peptides can directly enter cells is an important manifestation of their biological activity. Small molecule peptides can directly enter cells through the skin barrier, blood-brain barrier, placental barrier, and gastrointestinal mucosal barrier. Small molecule peptides are very active, and often a small amount can play a big role.

[0005] In modern society, male infertility is becoming increasingly prominent due to the influence of various factors such as increased life pressure, environmental pollution, and bad living habits. Low sperm motility is one of the important causes of male infertility. Currently, clinical methods for treating low sperm motility have limited efficacy and large side effects. Therefore, it is of great significance to develop a natural, efficient and safe composition for improving sperm motility. Summary of the invention

[0006] The purpose of the present invention is to provide an active peptide composition for improving male sperm motility and a preparation method thereof, which can effectively improve male sperm motility through the synergistic effect of a specific active peptide combination, and is natural, safe and has no side effects.

[0007] To achieve the above object, the present invention provides an active peptide composition for improving male sperm motility, comprising the following raw material components in parts by weight: 15-18 parts of oyster peptide, 10-28 parts of sea cucumber peptide, 6-10 parts of cordyceps peptide, 10-12 parts of fish white peptide, 15-25 parts of glutathione, 10-20 parts of wheat peptide, and 0.2-0.4 parts of coenzyme Q10.

[0008] A method for preparing an active peptide composition for improving male sperm motility comprises the following steps: Weigh oyster peptide, sea cucumber peptide, cordyceps peptide, fish white peptide, glutathione, wheat peptide, and coenzyme Q10 by weight, mix well, pre-freeze at -40 to -45°C for 2-3h, and dry at a vacuum degree of 0.05-0.08Pa and a temperature of -20 to -26°C for 36-40h to prepare a freeze-dried powder.

[0009] Preferably, the preparation method of glutathione is: S1. Select Saccharomyces cerevisiae as the strain and inoculate it into the seed culture medium. Cultivate it for 20-24 hours at 28-30°C, pH 5.5-5.8, and stirring speed 180-210 rpm to obtain seed solution. S2, inoculating the seed liquid into the fermentation medium at an inoculum rate of 8-15% for fermentation culture, adding a metal salt solution during the fermentation process, controlling the temperature at 32-35° C., maintaining the pH at 5.5-5.8, and fermenting for 50-70 hours; S3. After the fermentation is completed, the bacteria are collected by centrifugation, and the bacterial cells are broken by ultrasound to release glutathione, and then the glutathione is extracted and purified by ultrafiltration membrane filtration to obtain glutathione product.

[0010] Preferably, in step S1, the seed culture medium includes 20-25 g / L glucose, 5-10 g / L yeast extract, 10-15 g / L peptone, 0.5-0.7 g / L magnesium sulfate, and 1.2-1.8 g / L potassium dihydrogen phosphate.

[0011] Preferably, in step S1, a metal salt solution is added when the fermentation is carried out for 20-24 hours, the concentration of the metal salt solution is 0.1-0.2 g / L, and the metal salt solution includes one or more of magnesium sulfate, manganese sulfate, and zinc sulfate.

[0012] Preferably, in step S2, the fermentation medium includes 30-40 g / L glucose, 8-10 g / L glutamic acid, 6 g / L cysteine, 2.5-4 g / L glycine, and 2.3-2.5 g / L potassium dihydrogen phosphate.

[0013] The advantages and beneficial effects of the present invention using the above-mentioned active peptide composition for improving male sperm motility and the preparation method thereof are: Glutathione works with oyster peptide, sea cucumber peptide, Cordyceps sinensis peptide, fish white peptide and coenzyme Q10 to prevent cell oxidation, help protect cells from damage by free radicals, and improve immune function. Glutathione is also involved in the production and conduction of sperm, playing a role in improving sperm motility.

[0014] The coordination of coenzyme Q10, coenzyme Q10 (CoenzymeQ10, CoQ10), also known as ubiquinone (UQ), coenzyme Q (CoenzymeQ, CoQ), is a coenzyme present in all eukaryotic organisms that perform aerobic respiration. It is a benzoquinone fat-soluble compound with a structure similar to vitamin K. Q represents the quinone group, and 10 represents the number of isoprene attached to its tail. It is mainly formed in the inner membrane of mitochondria, and its main function is to drive human cells to produce energy. Coenzyme Q10 is mainly involved in the mitochondrial oxidative phosphorylation and the production of adenosine triphosphate, regulating the cellular redox environment, carrying reduced electrons into vesicles or out of cells during the electron membrane penetration process, and participating in the formation of proton gradients in the inner membrane and plasma membrane. It can accelerate cell renewal and stimulate cell activity, thereby greatly promoting the ability of cells to absorb nutrients. Coenzyme Q10 is a powerful antioxidant that can increase mitochondrial energy production, thereby improving sperm motility. Coenzyme Q10 supplements can significantly improve sperm motility and morphology.

[0015] Wheat peptides contain a variety of amino acids, which can enhance the activity of antioxidant enzymes, eliminate free radicals in the body, and inhibit lipid peroxidation. The glutamine contained in wheat oligopeptides can increase the number of lymphocytes in the body, improve the body's resistance to external pathogens, and enhance immunity.

[0016] The various amino acids and trace elements in fish white peptide can enhance the body's immunity and improve resistance; fish white peptide has an antioxidant effect, can remove free radicals in the body, delay cell aging, and protect cells from oxidative damage; zinc and other trace elements in fish white peptide have a positive effect on male reproductive health, can improve the quality and quantity of sperm, help regulate endocrine balance, and improve the function of the reproductive system.

[0017] The composition of the present invention is based on multiple specific active peptides, is natural, safe and has no side effects, can effectively improve male sperm motility, can improve male sexual function in a fatigued state, can increase serum testosterone levels, and has the characteristics of high efficiency, safety and stability. The application of the composition in the treatment of improving male sperm motility can improve the level of male hormones and sexual ability, and improve the quality of life.

[0018] The technical solution of the present invention is further described in detail below through embodiments. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is further illustrated by the following embodiments.

[0020] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0021] Unless otherwise defined, the instruments, reagents, materials, etc. involved in the present invention are all conventional instruments, reagents, materials, etc. already in the prior art and can be obtained through regular commercial channels. The experimental methods, detection methods, etc. involved in the present invention, unless otherwise specified, are all conventional experimental methods, detection methods, etc. already in the prior art.

[0022] The active peptide composition of the present invention is not used for diagnosis or treatment of diseases.

[0023] Example 1 An active peptide composition for improving male sperm motility comprises the following raw material components in parts by weight: 15 parts of oyster peptide, 15 parts of sea cucumber peptide, 6 parts of cordyceps peptide, 10 parts of fish white peptide, 15 parts of glutathione, 10 parts of wheat peptide and 0.2 parts of coenzyme Q10.

[0024] A method for preparing an active peptide composition for improving male sperm motility comprises the following steps: Oyster peptide, sea cucumber peptide, cordyceps peptide, fish white peptide, glutathione, wheat peptide, and coenzyme Q10 were weighed by weight, mixed evenly, pre-frozen at -45°C for 2h, and dried at a vacuum degree of 0.05Pa and a temperature of -20°C for 36h to prepare a freeze-dried powder.

[0025] The preparation method of glutathione is: S1. Select Saccharomyces cerevisiae as the strain and inoculate it into a seed culture medium, wherein the seed culture medium includes 20 g / L glucose, 5 g / L yeast extract, 15 g / L peptone, 0.5 g / L magnesium sulfate, and 1.2 g / L potassium dihydrogen phosphate. Cultivate for 20 hours at 28°C, pH 5.5, and a stirring speed of 180 rpm to obtain a seed solution. S2, inoculating the seed liquid into the fermentation medium at an inoculum rate of 15% for fermentation culture, the fermentation medium comprising 35 g / L glucose, 10 g / L glutamic acid, 6 g / L cysteine, 2.5 g / L glycine, and 2.3 g / L potassium dihydrogen phosphate, adding 0.1 g / L magnesium sulfate metal salt solution when the fermentation is carried out for 20 hours, controlling the temperature at 35° C., maintaining the pH at 5.5, and the fermentation time is 50 hours in total; S3. After the fermentation is completed, the bacteria are collected by centrifugation, and the bacterial cells are broken by ultrasound to release glutathione, and then the glutathione is extracted and purified by ultrafiltration membrane filtration to obtain glutathione product.

[0026] Example 2 An active peptide composition for improving male sperm motility comprises the following raw material components in parts by weight: 18 parts of oyster peptide, 10 parts of sea cucumber peptide, 9 parts of cordyceps militaris peptide, 12 parts of fish white peptide, 25 parts of glutathione, 12 parts of wheat peptide and 0.3 parts of coenzyme Q10.

[0027] A method for preparing an active peptide composition for improving male sperm motility comprises the following steps: Oyster peptide, sea cucumber peptide, cordyceps peptide, fish white peptide, glutathione, wheat peptide, and coenzyme Q10 were weighed by weight, mixed evenly, pre-frozen at -40°C for 3 h, and dried at a vacuum degree of 0.08 Pa and a temperature of -25°C for 40 h to prepare a freeze-dried powder.

[0028] The preparation method of glutathione is: S1. Select Saccharomyces cerevisiae as the strain and inoculate it into a seed culture medium, wherein the seed culture medium includes 25 g / L glucose, 8 g / L yeast extract, 10 g / L peptone, 0.7 g / L magnesium sulfate, and 1.5 g / L potassium dihydrogen phosphate. Cultivate for 24 hours at 28°C, pH 5.5, and a stirring speed of 200 rpm to obtain a seed solution. S2, inoculating the seed liquid into the fermentation medium at a 10% inoculum amount for fermentation culture, the fermentation medium includes 30g / L glucose, 8g / L glutamic acid, 6g / L cysteine, 3g / L glycine, and 2.5g / L potassium dihydrogen phosphate, adding 0.2g / L manganese sulfate metal salt solution when the fermentation is carried out for 24h, controlling the temperature at 32°C, maintaining the pH at 5.8, and the fermentation time is 65 hours in total; S3. After the fermentation is completed, the bacteria are collected by centrifugation, and the bacterial cells are broken by ultrasound to release glutathione, and then the glutathione is extracted and purified by ultrafiltration membrane filtration to obtain glutathione product.

[0029] Example 3 An active peptide composition for improving male sperm motility comprises the following raw material components in parts by weight: 18 parts of oyster peptide, 28 parts of sea cucumber peptide, 10 parts of cordyceps peptide, 11 parts of fish white peptide, 25 parts of glutathione, 20 parts of wheat peptide and 0.4 parts of coenzyme Q10.

[0030] A method for preparing an active peptide composition for improving male sperm motility comprises the following steps: Oyster peptide, sea cucumber peptide, cordyceps peptide, fish white peptide, glutathione, wheat peptide and coenzyme Q10 were weighed by weight, mixed evenly, pre-frozen at -45°C for 3 h, dried at a vacuum degree of 0.08 Pa and a temperature of -26°C for 40 h to prepare freeze-dried powder.

[0031] The preparation method of glutathione is: S1. Select Saccharomyces cerevisiae as the strain and inoculate it into a seed culture medium, wherein the seed culture medium includes 25 g / L glucose, 10 g / L yeast extract, 12 g / L peptone, 0.5 g / L magnesium sulfate, and 1.8 g / L potassium dihydrogen phosphate. Culture for 20 hours at 30°C, pH 5.8, and a stirring speed of 210 rpm to obtain a seed solution. S2, inoculating the seed liquid into the fermentation medium at an inoculum rate of 8% for fermentation culture, the fermentation medium comprising 40 g / L glucose, 10 g / L glutamic acid, 6 g / L cysteine, 4 g / L glycine, and 2.5 g / L potassium dihydrogen phosphate, adding 0.2 g / L zinc sulfate metal salt solution when the fermentation is carried out for 22 hours, controlling the temperature at 35° C., maintaining the pH at 5.5, and the fermentation time is 70 hours in total; S3. After the fermentation is completed, the bacteria are collected by centrifugation, and the bacterial cells are broken by ultrasound to release glutathione, and then the glutathione is extracted and purified by ultrafiltration membrane filtration to obtain glutathione product.

[0032] Comparative Example 1 An active peptide composition for improving male sperm motility comprises the following raw material components in parts by weight: 15 parts of oyster peptide, 15 parts of sea cucumber peptide, 6 parts of cordyceps peptide, 10 parts of chlorella powder, 15 parts of grape seed powder, 10 parts of yeast polypeptide and 10 parts of wheat peptide.

[0033] A method for preparing an active peptide composition for improving male sperm motility comprises the following steps: Oyster peptide, sea cucumber peptide, cordyceps peptide, chlorella powder, grape seed powder, yeast peptide and wheat peptide were weighed by weight, mixed evenly, pre-frozen at -45°C for 2h, dried at a vacuum degree of 0.05Pa and a temperature of -20°C for 36h to prepare freeze-dried powder.

[0034] Comparative Example 2 An active peptide composition for improving male sperm motility comprises the following raw material components in parts by weight: 15 parts of oyster peptide, 15 parts of sea cucumber peptide, 6 parts of cordyceps peptide and 10 parts of wheat peptide.

[0035] A method for preparing an active peptide composition for improving male sperm motility comprises the following steps: Oyster peptide, sea cucumber peptide, cordyceps peptide and wheat peptide were weighed by weight and mixed evenly, pre-frozen at -45°C for 2h, and dried at a vacuum degree of 0.05Pa and a temperature of -20°C for 36h to prepare freeze-dried powder.

[0036] Comparative Example 3 An active peptide composition for improving male sperm motility comprises the following raw material components in parts by weight: 15 parts of oyster peptide, 15 parts of sea cucumber peptide, 6 parts of cordyceps peptide, 10 parts of fish white peptide, 15 parts of glutathione, 10 parts of wheat peptide and 0.2 parts of coenzyme Q10.

[0037] A method for preparing an active peptide composition for improving male sperm motility comprises the following steps: Oyster peptide, sea cucumber peptide, cordyceps peptide, fish white peptide, glutathione, wheat peptide, and coenzyme Q10 were weighed by weight, mixed evenly, pre-frozen at -45°C for 2h, and dried at a vacuum degree of 0.05Pa and a temperature of -20°C for 36h to prepare a freeze-dried powder.

[0038] The preparation method of glutathione is: S1. Select Saccharomyces cerevisiae as the strain and inoculate it into a seed culture medium, wherein the seed culture medium includes 20 g / L glucose, 5 g / L yeast extract, 15 g / L peptone, 0.5 g / L magnesium sulfate, and 1.2 g / L potassium dihydrogen phosphate. Cultivate for 20 hours at 28°C, pH 5.5, and a stirring speed of 180 rpm to obtain a seed solution. S2, inoculating the seed liquid into the fermentation medium at an inoculum of 15% for fermentation culture, the fermentation medium comprising 35 g / L glucose, 10 g / L glutamic acid, 6 g / L cysteine, 2.5 g / L glycine, and 2.3 g / L potassium dihydrogen phosphate, controlling the temperature at 35° C., maintaining the pH at 5.5, and fermenting for a total of 50 hours; S3. After the fermentation is completed, the bacteria are collected by centrifugation, and the bacterial cells are broken by ultrasound to release glutathione, and then the glutathione is extracted and purified by ultrafiltration membrane filtration to obtain glutathione product.

[0039] Experimental example (1) Experiment to measure the effect on anti-fatigue effect 70 adult male ICR mice were divided into 7 groups, 10 mice in each group, and the active peptide compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 3 were respectively administered to adult male ICR mice for 45 days to evaluate their performance. A blank control group (administered with an equal amount of sterilized water) was also set up.

[0040] The active peptide composition of Example 1-3 and Comparative Example 1-3 was prepared into a solution at 10 mg / mL, and 1 mL / day was administered to mice for every 15 g of body weight. The weight-bearing swimming exhaustion time of mice in different groups was measured, and the results are shown in Table 1.

[0041] Table 1 Test results of the effect of anti-fatigue effect ;

[0042] (2) Serum hormone level test Blood was collected from the ocular venous plexus of each group of mice, and after standing at room temperature for 1 hour, it was centrifuged at 4°C and 3500r / min for 15 minutes to obtain serum, which was stored in a -80°C refrigerator for testing. The levels of testosterone (T), luteinizing hormone (LH), and dihydrotestosterone (DHT) in the serum were detected. The test results are shown in Table 2.

[0043] Table 2 Mean results of serum hormone level experiments ;

[0044] (3) Sperm motility test The sperm of mice in each group was collected from the tail of epididymis. Then, the sperm concentration and motility were measured by computer-aided sperm analysis (CASA) analysis system. The results are shown in Table 3.

[0045] Table 3 Test results ; As shown in Table 3, the active peptide composition of the present invention can significantly improve sperm motility. No leucopeptide, glutathione and coenzyme Q10 were added in Comparative Example 2, resulting in the worst sperm motility improvement effect in Comparative Example 2. In Comparative Example 1, yeast polypeptide, grape seed powder and chlorella powder were used instead of leucopeptide, glutathione and coenzyme Q10. As shown in Tables 1 to 3, the effect of Comparative Example 1 is worse than that of Examples 1 to 3.

[0046] Therefore, the present invention adopts the above-mentioned active peptide composition and preparation method for improving male sperm motility, and effectively improves male sperm motility through the synergistic effect of a specific active peptide combination, and is naturally safe and has no side effects.

[0047] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. An active peptide composition for improving male sperm motility, characterized in that: The invention comprises the following raw material components in parts by weight: 15-18 parts of oyster peptide, 10-28 parts of sea cucumber peptide, 6-10 parts of cordyceps peptide, 10-12 parts of fish white peptide, 15-25 parts of glutathione, 10-20 parts of wheat peptide and 0.2-0.4 parts of coenzyme Q10.

2. The method for preparing an active peptide composition for improving male sperm motility according to claim 1, characterized in that: The steps include: Weigh oyster peptide, sea cucumber peptide, cordyceps peptide, fish white peptide, glutathione, wheat peptide, and coenzyme Q10 by weight, mix well, pre-freeze at -40 to -45°C for 2-3h, and dry at a vacuum degree of 0.05-0.08Pa and a temperature of -20 to -26°C for 36-40h to prepare a freeze-dried powder.

3. The method for preparing an active peptide composition for improving male sperm motility according to claim 2, characterized in that: The preparation method of the glutathione is: S1. Select Saccharomyces cerevisiae as the strain and inoculate it into the seed culture medium. Cultivate it for 20-24 hours at 28-30°C, pH 5.5-5.8, and stirring speed 180-210 rpm to obtain seed solution. S2, inoculating the seed liquid into the fermentation medium at an inoculum rate of 8-15% for fermentation culture, adding a metal salt solution during the fermentation process, controlling the temperature at 32-35° C., maintaining the pH at 5.5-5.8, and fermenting for 50-70 hours; S3. After the fermentation is completed, the bacteria are collected by centrifugation, and the bacterial cells are broken by ultrasound to release glutathione, and then the glutathione is extracted and purified by ultrafiltration membrane filtration to obtain glutathione product.

4. The method for preparing an active peptide composition for improving male sperm motility according to claim 3, characterized in that: In step S1, the seed culture medium includes 20-25 g / L of glucose, 5-10 g / L of yeast extract, 10-15 g / L of peptone, 0.5-0.7 g / L of magnesium sulfate, and 1.2-1.8 g / L of potassium dihydrogen phosphate.

5. The method for preparing an active peptide composition for improving male sperm motility according to claim 3, characterized in that: In step S2, a metal salt solution is added when the fermentation is carried out for 20-24 hours, the concentration of the metal salt solution is 0.1-0.2 g / L, and the metal salt solution includes one or more of magnesium sulfate, manganese sulfate, and zinc sulfate.

6. The method for preparing an active peptide composition for improving male sperm motility according to claim 3, characterized in that: In step S2, the fermentation medium includes 30-40 g / L of glucose, 8-10 g / L of glutamic acid, 6 g / L of cysteine, 2.5-4 g / L of glycine, and 2.3-2.5 g / L of potassium dihydrogen phosphate.