Liquid dressing and preparation method thereof

Abstract

The invention relates to the technical field of liquid dressings, and provides a liquid dressing and a preparation method thereof.The liquid dressing is prepared from, by mass, 2-5 parts of chitosan, 0.2-2 parts of antibacterial agent, 0.1-2 parts of growth factors, 0.01-0.2 part of auxiliary materials and 90-100 parts of solvent; wherein the antibacterial agent comprises propolis, a green tea extract and nano silver oxide particles; the growth factors comprise epidermal growth factors and platelet-derived growth factors; the solvent comprises a mixture of acidic electrolyzed oxidizing water and medical purified water. By adopting the liquid dressing, comprehensive protection and healing support can be provided for wounds, infection can be effectively resisted, tissue repair can be promoted, a moist environment can be kept, and therefore the healing process is accelerated.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid dressings, and in particular to a liquid dressing and a preparation method thereof. Background Art

[0002] Traditional wound care products such as band-aids and gauze have the disadvantages of strong foreign body sensation, easy adhesion to wounds, easy to fall off after getting wet, and cannot avoid wound scabs, and poor waterproof and breathable properties. In the 1960s, the theory of moist healing was proposed, pointing out that a moist environment can increase the migration speed of epidermal cells, making the wound healing time shorter than the time for wound scabs in a dry environment; at the same time, a moist microenvironment makes granulation tissue grow faster, and the epithelial cells in moist wounds grow thicker and regenerate more. This microenvironment also alleviates the pain caused to patients by the adhesion of dressings to the wound, protects the nerve tissue of the wound, and promotes cell proliferation and capillary formation, thereby promoting wound healing.

[0003] However, although many liquid dressings contain antibacterial ingredients, their antibacterial effects are limited by the concentration and release rate of the active ingredients, and they may not be able to inhibit bacteria for a long time or kill bacteria completely.

[0004] In view of this, a liquid dressing and a preparation method thereof are urgently needed. Summary of the invention

[0005] The object of the present invention is to provide a liquid dressing and a preparation method thereof in view of the deficiencies in the prior art.

[0006] To achieve the above object, the technical solution adopted by the present invention is:

[0007] The first aspect of the present invention is to provide a liquid dressing, comprising, by weight:

[0008] Chitosan: 2-5 parts,

[0009] Antibacterial agent: 0.2-2 parts,

[0010] Growth factors: 0.1-2 parts,

[0011] Excipients: 0.01-0.2 parts,

[0012] Solvent: 90 parts - 100 parts;

[0013] The antibacterial agent includes propolis, green tea extract, and nano silver oxide particles; the growth factor includes epidermal growth factor and platelet-derived growth factor; and the solvent includes a mixture of acidic oxidative potential water and medical purified water.

[0014] Preferably, the mass ratio of acidic oxidative potential water to medical purified water is 1:(2-5); or / and the auxiliary materials include: at least one of glycerol, sodium hyaluronate, carbomer, and chlorhexidine.

[0015] Preferably, the pH of the liquid dressing is 5-6; or / and the water vapor permeability of the liquid dressing is greater than 500 g / (m 2 ×24h); or / and the relative density of liquid dressing is 0.9g / cm 3 -1.1g / cm 3 ; or / and the viscosity of the liquid dressing is less than 100 cP.

[0016] A second aspect of the present invention is to provide a method for preparing a liquid dressing, comprising:

[0017] S100, preparing a chitosan solution: adding chitosan to a solvent and performing a first stirring treatment to obtain a chitosan solution;

[0018] S200, preparing a liquid dressing: adding microspheres containing an antibacterial agent, microcapsules containing growth factors, and auxiliary materials into a chitosan solution, and performing a second stirring process to obtain a liquid dressing;

[0019] The antibacterial agent includes propolis, green tea extract, and nano silver oxide particles; the growth factor includes epidermal growth factor and platelet-derived growth factor; the solvent includes a mixture of acidic oxidative potential water and medical purified water; and the preparation method of the microcapsule containing the growth factor includes:

[0020] S201, adding natural polysaccharide to acetic acid aqueous solution, stirring until the natural polysaccharide is dissolved, to obtain a natural polysaccharide solution;

[0021] S202, adding the growth factor and the cross-linking agent into the natural polysaccharide solution, stirring evenly, and then centrifuging to obtain microcapsules containing the growth factor.

[0022] Furthermore, in S100, the solvent is a 2% acetic acid solution; and the time of the first stirring treatment is 1 h-2 h.

[0023] Preferably, the natural polysaccharide comprises any one of chitosan, cellulose, pectin, and sodium alginate; or / and the cross-linking agent comprises sodium tripolyphosphate or calcium chloride.

[0024] Preferably, the concentration of the natural polysaccharide solution is 5 g / L-20 g / L; or / and the concentration of the acetic acid aqueous solution is 5 g / L-10 g / L.

[0025] Preferably, the method for preparing microspheres containing an antibacterial agent comprises:

[0026] S211, adding the antibacterial agent to the acetic acid solution of nano-chitosan, and sequentially performing homogenization and freeze-drying to obtain microspheres containing the antibacterial agent.

[0027] Preferably, the concentration of the nano-chitosan acetic acid solution is 5 g / L-10 g / L; the homogenization treatment is performed under ultrasonic conditions; and the diameter of the microspheres containing the antibacterial agent is 1 μm-5 μm.

[0028] Preferably, in S200, after the second stirring process, the following steps are further performed:

[0029] Sterilization treatment: sterilization treatment is carried out by filtering and sterilization using a microfiltration membrane.

[0030] The present invention adopts the above technical solution, and has the following technical effects compared with the prior art:

[0031] The liquid dressing of the present invention and its preparation method can effectively inhibit the growth of broad-spectrum bacteria and fungi through the synergistic effect of multiple antibacterial ingredients, significantly reduce the risk of wound infection, and help accelerate the cleaning and healing of wounds; growth factors can stimulate cell proliferation and migration, help the regeneration of epithelial cells and fibroblasts, and accelerate tissue repair and healing; chitosan has good hygroscopicity and film-forming properties, can form a moisturizing film on the wound surface to maintain a moist healing environment, and has good air permeability, can prevent the wound from drying and scabbing, and help cell migration; acidic oxidative potential water itself has a certain antibacterial effect, can further inhibit the growth of pathogenic microorganisms, and by adjusting the ratio of acidic oxidative potential water and pure water, the pH value of the solution is controlled between 5 and 6, which is close to the natural acidic environment of the skin, reduces irritation and helps maintain the skin barrier function; the liquid dressing of the present invention can provide comprehensive protection and healing support for wounds, can not only effectively resist infection, but also promote tissue repair, maintain a moist environment, and thus accelerate the healing process. DETAILED DESCRIPTION

[0032] The specific embodiments of the present invention will be described in detail below.

[0033] Unless otherwise defined, technical or scientific terms used in the claims and the specification shall have the common meanings understood by persons having ordinary skills in the technical field to which the present invention belongs.

[0034] The words "include" or similar words used in the patent application specification and claims of the present invention mean that the items appearing before "include" include the items listed after "include" or their equivalents, and do not exclude other items.

[0035] The numerical values ​​mentioned in the present invention include all numerical values ​​that increase from low to high by one unit, assuming that there are at least two units between any lower value and the higher value. For example, if a component or a physical quantity is said to be from 1 to 100, 10 to 90 is more preferred, and 20 to 80 is the most preferred, it is intended to express that values ​​such as 5 to 95, 14 to 76, 23 to 67, 32 to 58, 41 to 49 are clearly listed in this specification; for values ​​less than 1, 0.0001, 0.001, 0.01 or 0.1 are considered to be more suitable as a unit. The above examples are for illustrative purposes only. In fact, all numerical combinations between the lowest value and the highest value listed are deemed to be clearly listed in this specification in a similar manner.

[0036] Example 1

[0037] This embodiment provides a liquid dressing, which comprises, by weight:

[0038] Chitosan: 2 parts,

[0039] Antibacterial agent: 0.2 parts,

[0040] Growth factors: 0.1 parts,

[0041] Excipients: 0.01 parts,

[0042] Solvent: 90 parts;

[0043] The antibacterial agent includes propolis, green tea extract, and nano silver oxide particles; the growth factor includes epidermal growth factor and platelet-derived growth factor; the solvent includes a mixture of acidic oxidation potential water and medical purified water, and the mass ratio of acidic oxidation potential water to medical purified water is 1:2; the auxiliary materials include glycerol and carbomer; the preparation method of the liquid dressing includes:

[0044] S100, preparing a chitosan solution: adding chitosan to a 2% acetic acid solution, and performing a first stirring treatment for 1 hour to obtain a chitosan solution;

[0045] S200, preparing a liquid dressing: adding microspheres with a diameter of 1 μm containing an antibacterial agent, microcapsules containing growth factors, glycerol and carbomer to a chitosan solution, and performing a second stirring treatment to obtain a liquid dressing;

[0046] The microspheres containing the antibacterial agent are purchased from outside, and the preparation method of the microcapsules containing the growth factor includes:

[0047] S201, adding chitosan to an aqueous acetic acid solution having a concentration of 5 g / L, stirring until the natural polysaccharide is dissolved, to obtain a natural polysaccharide solution having a concentration of 5 g / L;

[0048] S202, adding the growth factor and the cross-linking agent into the natural polysaccharide solution, stirring evenly, and then centrifuging to obtain microcapsules containing the growth factor.

[0049] Example 2

[0050] This embodiment provides a liquid dressing, which comprises, by weight:

[0051] Chitosan: 5 parts,

[0052] Antimicrobial agent: 2 parts,

[0053] Growth Factors: 2 parts,

[0054] Excipients: 0.2 parts,

[0055] Solvent: 100 parts;

[0056] The antibacterial agent includes propolis, green tea extract, and nano silver oxide particles; the growth factor includes epidermal growth factor and platelet-derived growth factor; the solvent includes a mixture of acidic oxidation potential water and medical purified water, and the mass ratio of acidic oxidation potential water to medical purified water is 1:5; the auxiliary materials include glycerol, chlorhexidine and carbomer; the preparation method of the liquid dressing includes:

[0057] S100, preparing a chitosan solution: adding chitosan to a 2% acetic acid solution, and performing a first stirring treatment for 2 hours to obtain a chitosan solution;

[0058] S200, preparing a liquid dressing: adding microspheres with a diameter of 5 μm containing an antibacterial agent, microcapsules containing growth factors, glycerol, chlorhexidine and carbomer to a chitosan solution, performing a second stirring treatment, and then performing a filtering and sterilization treatment using a microfiltration membrane to obtain a liquid dressing;

[0059] The microspheres containing the antibacterial agent are purchased from outside, and the preparation method of the microcapsules containing the growth factor includes:

[0060] S201, adding cellulose to an aqueous acetic acid solution with a concentration of 10 g / L, stirring until the natural polysaccharide is dissolved, to obtain a natural polysaccharide solution with a concentration of 20 g / L;

[0061] S202, adding the growth factor and the cross-linking agent into the natural polysaccharide solution, stirring evenly, and then centrifuging to obtain microcapsules containing the growth factor.

[0062] Example 3

[0063] This embodiment provides a liquid dressing, which comprises, by weight:

[0064] Chitosan: 3 parts,

[0065] Antimicrobial agent: 1 part,

[0066] Growth Factors: 1 serving,

[0067] Excipients: 0.1 parts,

[0068] Solvent: 95 parts;

[0069] The antibacterial agent includes propolis, green tea extract, and nano silver oxide particles; the growth factor includes epidermal growth factor and platelet-derived growth factor; the solvent includes a mixture of acidic oxidative potential water and medical purified water, and the mass ratio of acidic oxidative potential water to medical purified water is 1:5; the auxiliary materials include sodium hyaluronate, carbomer and chlorhexidine; the preparation method of the liquid dressing includes:

[0070] S100, preparing a chitosan solution: adding chitosan to a 2% acetic acid solution, and performing a first stirring treatment for 1 hour to obtain a chitosan solution;

[0071] S200, preparing a liquid dressing: adding microspheres with a diameter of 3 μm containing an antibacterial agent, microcapsules containing growth factors, sodium hyaluronate, chlorhexidine and carbomer to a chitosan solution, performing a second stirring treatment, and then performing a filtration and sterilization treatment using a microfiltration membrane to obtain a liquid dressing;

[0072] The method for preparing microcapsules containing growth factors comprises:

[0073] S201, adding cellulose to an aqueous acetic acid solution having a concentration of 7 g / L, stirring until the natural polysaccharide is dissolved, to obtain a natural polysaccharide solution having a concentration of 15 g / L;

[0074] S202, adding the growth factor and the cross-linking agent to the natural polysaccharide solution, stirring evenly, and then centrifuging to obtain microcapsules containing the growth factor;

[0075] The method for preparing microspheres containing an antimicrobial agent comprises:

[0076] S211, adding the antibacterial agent to the acetic acid solution of nano-chitosan with a concentration of 5 g / L, homogenizing under ultrasonic conditions, and then freeze-drying to obtain microspheres containing the antibacterial agent.

[0077] Example 4

[0078] This embodiment provides a liquid dressing, which comprises, by weight:

[0079] Chitosan: 4 parts,

[0080] Antimicrobial agent: 2 parts,

[0081] Growth Factors: 2 parts,

[0082] Excipients: 0.2 parts,

[0083] Solvent: 90 parts;

[0084] The antibacterial agent includes propolis, green tea extract, and nano silver oxide particles; the growth factor includes epidermal growth factor and platelet-derived growth factor; the solvent includes a mixture of acidic oxidation potential water and medical purified water, and the mass ratio of acidic oxidation potential water to medical purified water is 1:5; the auxiliary materials include glycerol, carbomer and chlorhexidine; the preparation method of the liquid dressing includes:

[0085] S100, preparing a chitosan solution: adding chitosan to a 2% acetic acid solution, and performing a first stirring treatment for 2 hours to obtain a chitosan solution;

[0086] S200, preparing a liquid dressing: adding microspheres with a diameter of 3 μm containing an antibacterial agent, microcapsules containing growth factors, glycerol, chlorhexidine and carbomer to a chitosan solution, performing a second stirring treatment, and then performing a filtering and sterilization treatment using a microfiltration membrane to obtain a liquid dressing;

[0087] The method for preparing microcapsules containing growth factors comprises:

[0088] S201, adding cellulose to an acetic acid aqueous solution with a concentration of 8 g / L, stirring until the natural polysaccharide is dissolved, to obtain a natural polysaccharide solution with a concentration of 15 g / L;

[0089] S202, adding the growth factor and the cross-linking agent to the natural polysaccharide solution, stirring evenly, and then centrifuging to obtain microcapsules containing the growth factor;

[0090] The method for preparing microspheres containing an antimicrobial agent comprises:

[0091] S211, adding the antibacterial agent to the acetic acid solution of nano-chitosan with a concentration of 10 g / L, homogenizing under ultrasonic conditions, and then freeze-drying to obtain microspheres containing the antibacterial agent.

[0092] Performance Testing

[0093] The performance tests were performed on the liquid dressings in Examples 1-4. The test results are shown in Table 1.

[0094] Viscosity test: The viscosity of the liquid dressings in Examples 1-4 was measured using a rotational viscometer;

[0095] pH test: The pH of the liquid dressings in Examples 1-4 was measured using a pH meter;

[0096] Water vapor permeability test: The water vapor permeability of the liquid dressings in Examples 1-4 was measured using a water vapor permeability tester;

[0097] Antibacterial test: The antibacterial rate of the liquid dressings in Examples 1-4 was determined by the plate coating inhibition zone method.

[0098] Table 1

[0099]

[0100] It can be seen from Table 1 that the liquid dressings in Examples 1-4 have suitable viscosity and pH, good air permeability and antibacterial rate, can effectively inhibit the growth of microorganisms at the wound, and have good air permeability, thereby effectively promoting wound healing; the liquid dressings in Examples 3-4 have better performance than the liquid dressings in Examples 1-2, which may be due to the use of more suitable parameters in the preparation methods of Examples 3-4 and the preparation method of the microspheres containing antibacterial agents provided by the present invention, thereby obtaining better antibacterial properties.

[0101] In summary, the liquid dressing of the present invention and its preparation method can effectively inhibit the growth of broad-spectrum bacteria and fungi through the synergistic effect of multiple antibacterial ingredients, significantly reduce the risk of wound infection, and help accelerate wound cleaning and healing; growth factors can stimulate cell proliferation and migration, help the regeneration of epithelial cells and fibroblasts, and accelerate tissue repair and healing; chitosan has good hygroscopicity and film-forming properties, can form a moisturizing film on the wound surface, maintain a moist healing environment, and has good air permeability, can prevent wound drying and scabbing, and help cell migration; acidic oxidative potential water itself has a certain antibacterial effect, which can further inhibit the growth of pathogenic microorganisms. By adjusting the ratio of acidic oxidative potential water and pure water, the pH value of the solution is controlled between 5-6, which is close to the natural acidic environment of the skin, reduces irritation and helps maintain the skin barrier function; the liquid dressing of the present invention can provide comprehensive protection and healing support for wounds, which can not only effectively resist infection, but also promote tissue repair, maintain a moist environment, and thus accelerate the healing process.

[0102] The above are only preferred embodiments of the present invention, and are not intended to limit the implementation methods and protection scope of the present invention. Those skilled in the art should be aware that all solutions obtained by equivalent substitutions and obvious changes made using the contents of the specification of the present invention should be included in the protection scope of the present invention.

Claims

1. A liquid dressing, characterized in that: Calculated by mass, including: Chitosan: 2-5 parts, Antibacterial agent: 0.2-2 parts, Growth factors: 0.1-2 parts, Excipients: 0.01-0.2 parts, Solvent: 90 parts - 100 parts; Wherein, the antibacterial agent includes: propolis, green tea extract, and nano silver oxide particles; The growth factors include: epidermal growth factor and platelet-derived growth factor; The solvent includes: a mixture of acidic oxidizing potential water and medical purified water.

2. The liquid dressing according to claim 1, characterized in that The mass ratio of the acidic oxidizing potential water to the medical purified water is 1:(2-5); or / and The auxiliary materials include: at least one of glycerin, sodium hyaluronate, carbomer and chlorhexidine.

3. The liquid dressing according to any one of claims 1-2, characterized in that: The pH of the liquid dressing is 5-6; or / and The water vapor permeability of the liquid dressing is greater than 500 g / (m 2 ×24h); or / and The relative density of the liquid dressing is 0.9 g / cm 3 -1.1g / cm 3 ; or / and The viscosity of the liquid dressing is less than 100 cP.

4. A method for preparing a liquid dressing according to any one of claims 1 to 4, characterized in that: include: S100, preparing a chitosan solution: adding the chitosan into the solvent, performing a first stirring process, and obtaining the chitosan solution; S200, preparing a liquid dressing: adding the microspheres containing the antibacterial agent, the microcapsules containing the growth factor, and the auxiliary materials into the chitosan solution, and performing a second stirring process to obtain the liquid dressing; Wherein, the antibacterial agent includes: propolis, green tea extract, and nano silver oxide particles; The growth factors include: epidermal growth factor and platelet-derived growth factor; The solvent includes: a mixture of acidic oxidizing potential water and medical purified water; The preparation method of the microcapsule containing the growth factor comprises: S201, adding a natural polysaccharide to an aqueous acetic acid solution, and stirring until the natural polysaccharide is dissolved to obtain a natural polysaccharide solution; S202, adding the growth factor and the cross-linking agent into the natural polysaccharide solution, stirring evenly, and then centrifuging to obtain the microcapsules containing the growth factor.

5. The preparation method according to claim 4, characterized in that: The natural polysaccharide includes: any one of chitosan, cellulose, pectin, and sodium alginate; or / and The cross-linking agent includes sodium tripolyphosphate or calcium chloride.

6. The preparation method according to claim 4, characterized in that: The concentration of the natural polysaccharide solution is 5g / L-20g / L; or / and The concentration of the acetic acid aqueous solution is 5g / L-10g / L.

7. The preparation method according to claim 4, characterized in that: The method for preparing the microspheres containing the antibacterial agent comprises: S211, adding the antibacterial agent to the acetic acid solution of nano-chitosan, and sequentially performing homogenization and freeze-drying to obtain the microspheres containing the antibacterial agent.

8. The preparation method according to claim 7, characterized in that: The concentration of the nano-chitosan acetic acid solution is 5g / L-10g / L; The homogenization process is carried out under ultrasonic conditions; The diameter of the microspheres containing the antibacterial agent is 1 μm-5 μm.

9. The preparation method according to claim 4, characterized in that: In the S200, after the second stirring process, the following further comprises: Sterilization treatment, wherein the sterilization treatment is performed by filtering and sterilizing with a microfiltration membrane.

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