Anti-aging skin care composition prepared from algae oligosaccharide, carboxymethyl chitosan and hydrolyzed sodium hyaluronate and preparation process of anti-aging skin care composition

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging skin care composition prepared from algae oligosaccharide, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, and the anti-aging skin care composition comprises the algae oligosaccharide, the carboxymethyl chitosan and the hydrolyzed sodium hyaluronate. It has been found that the composition of the algae oligosaccharide, the carboxymethyl chitosan and the hydrolyzed sodium hyaluronate can promote synthesis of collagen I, collagen III and collagen IV of fibroblasts, the synthesis of the collagen I, collagen III and collagen IV can be promoted to be greatly increased through cooperation of the algae oligosaccharide, the carboxymethyl chitosan and the hydrolyzed sodium hyaluronate, and therefore the effects of delaying senescence, resisting wrinkles and tightening the skin can be achieved, and the composition can be used in anti-aging products.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and specifically relates to an anti-aging skin care composition prepared by utilizing algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, and a preparation process thereof. Background Art

[0002] Skin aging is a natural process. As time goes by, the skin will gradually lose its ability to repair itself; environmental damage damages elastin and collagen, reducing the production of collagen in the skin, making the adhesion protein connecting the dermis and epidermis fragile, and the production of collagen drops sharply. Collagen becomes fragile, the skin cannot store energy, and the ability to lock water gradually decreases. Skin aging mainly occurs in the dermis and manifests itself in the epidermis. Due to complex environmental factors such as ultraviolet rays, air pollution, and nicotine, epidermal cells will lead to a decrease in epidermal cell interstitium, slow division of keratin, accelerated keratinocyte aging and depression, and the epidermis will lose its luster, become rough and dark, and develop fine wrinkles. The synthesis of collagen fibers in the dermis tissue decreases, collagen is hydrolyzed, and the elastic fibers in the reticular layer of the dermis are denatured, resulting in collagen loss, dermis atrophy, sagging, and deep wrinkles. The emergence of anti-wrinkle cosmetics has brought good news to people who want younger skin and satisfied people's desire for younger skin. There are many anti-aging products on the market for different anti-aging channels, but most of them are derived from chemical synthesis methods, which is inconsistent with the concept of pure beauty advocated now. Although some plant anti-aging products have appeared on the market, the effect is not satisfactory or there is a problem of poor absorption. Therefore, it is urgent to develop an anti-aging product with less chemical synthetic ingredients and good effect to meet people's needs for green skin care. Summary of the invention

[0003] In order to solve the above problems, the present invention provides an anti-aging skin care composition prepared by using algae oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, which comprises algae oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate.

[0004] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6-10 parts, 0.3-0.8 parts and 5 parts respectively.

[0005] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6 parts, 0.8 parts and 5 parts respectively.

[0006] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6-10 parts, 3 parts and 5 parts respectively.

[0007] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 10 parts, 0.8 parts and 5 parts respectively.

[0008] Furthermore, the weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 10 parts, 3 parts and 5 parts respectively.

[0009] Furthermore, the anti-aging skin care composition also comprises auxiliary materials and preservatives.

[0010] Furthermore, the weight proportion of the preservative in the anti-aging skin care composition is 0.3-0.6 parts.

[0011] Furthermore, the preservative includes but is not limited to any one of 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid, caprylyl glycol, pentylene glycol and ethylhexylglycerin.

[0012] Furthermore, the auxiliary materials include deionized water and a thickener.

[0013] Furthermore, the weight proportion of the deionized water in the anti-aging skin care composition is 65-90 parts, and the weight proportion of the thickener in the anti-aging skin care composition is 1-2 parts.

[0014] Furthermore, the thickener includes any one of carbomer, xanthan gum, and ammonium acryloyldimethyltaurate / VP copolymer.

[0015] The present invention also provides a preparation process of the anti-aging skin care composition as described herein. When the anti-aging skin care composition consists only of algae oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, the raw materials only need to be mixed according to the formula; when the anti-aging skin care composition also contains auxiliary materials and preservatives, the preparation process comprises the following steps: weighing the raw materials according to the formula, adding algae oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate to deionized water in sequence, stirring to dissolve, then adding a thickener and stirring evenly, and finally adding a preservative and stirring evenly to obtain the anti-aging skin care composition.

[0016] The present invention also provides the use of the anti-aging skin care composition as described herein in anti-aging cosmetics.

[0017] The present invention also provides an anti-aging cosmetic comprising the anti-aging skin care composition as described herein.

[0018] Furthermore, the anti-aging cosmetics include but are not limited to essences, facial creams, facial masks, body lotions, etc.

[0019] Advantageous Effects of the Invention

[0020] The present invention has found that the composition of algal oligosaccharides, carboxymethyl deacetylated chitosan and hydrolyzed sodium hyaluronate can promote the synthesis of type I, III and IV collagen in fibroblasts. The three can work together to promote the synthesis of type I, III and IV collagen to increase to a large extent, thereby having the effects of delaying aging, anti-wrinkle and firming, and can therefore be used in anti-aging products. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Shown are the results of testing the effects of single ingredients on type I collagen production.

[0022] Figure 2 Shown are the results of testing the effects of single raw materials on type III collagen production.

[0023] Figure 3 Shown are the results of testing the effects of single raw materials on type IV collagen production.

[0024] Figure 4 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type I collagen production.

[0025] Figure 5 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type I collagen production.

[0026] Figure 6 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type III collagen production.

[0027] Figure 7 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type III collagen production.

[0028] Figure 8 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type IV collagen production.

[0029] Fig. 9 Shown are the results of testing the effects of single raw materials and raw materials in combination in different amounts on type IV collagen production.

[0030] Fig.10 The average value of the average width of the outer corner wrinkles tested in Example 2 at various time points before and after the use of the sample is shown.

[0031] Fig.11 The change rate of the average width of the outer corner wrinkles at various time points before and after the use of the sample tested in Example 2 is shown.

[0032] Fig.12 The average value of the average width of under-eye wrinkles at various time points before and after the use of the sample tested in Example 2 is shown.

[0033] Fig.13 The change rate of the average width of under-eye wrinkles at various time points before and after the use of the sample tested in Example 2 is shown.

[0034] Fig.14 An example of skin surface morphology analysis of the subjects tested in Example 2 before and after use of the samples is shown.

[0035] Fig.15 The results of the test on the effect of the raw material combination not described in the present invention on the production of type I collagen are shown. DETAILED DESCRIPTION

[0036] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0037] The algal oligosaccharides used were purchased from Qingdao Haida Marine Oligosaccharide Technology Co., Ltd., item number 84775-78-0, and are marine oligosaccharides extracted from algae (Giant algae) in the Antarctic waters of Chile; carboxymethyl deacetylated chitosan was purchased from Shanghai Chunlu Biochemistry Co., Ltd.; and hydrolyzed sodium hyaluronate was purchased from Shandong Guantianxia Biotechnology Co., Ltd.

[0038] Example 1: Effects of co-incubation of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate on the production of human type I collagen (Col I), human type III collagen (Col III) and human type IV collagen (Col IV) in human fibroblasts

[0039] Human skin fibroblasts were inoculated into MEM culture medium containing 5% fetal bovine serum by volume, cultured in a 5% CO2, 37°C incubator, and the medium was changed once every 3 days. After 7 days of culture, the original culture medium was aspirated, the cells were washed 3 times with PBS buffer, 0.5 mL of 0.25% Trypsin-1 mM EDTA4Na solution was added, and then 10 mL of MEM culture medium was added. The cells were suspended by pipetting, and then transferred to a centrifuge tube, centrifuged at 2000 rpm for 5 min, the supernatant was removed, and an appropriate amount of MEM culture medium containing 5% fetal bovine serum by volume was added. The cells were suspended by pipetting.

[0040] Take a 96-well plate and add 100 μL of cell suspension (containing 0.2×10 cells) to each well. 5), placed in a 5% CO2, 37°C incubator for 24h, discarded the culture medium in the 96-well plate, and added 100μL of a culture medium containing one, two or three of algae oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, wherein the final mass concentrations of algae oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the culture medium are set as follows: algae oligosaccharides are used in two gradients of 0.06% and 0.1%, carboxymethyl chitosan is used in two gradients of 0.003% and 0.008%, and hyaluronic acid is used at 0.05%, thereby constructing culture media containing a single raw material, a combination of two raw materials and a combination of three raw materials for treating human skin fibroblasts; and setting an equal volume of blank culture medium as a blank control group. The 96-well plate was then placed in a 5% CO2, 37°C incubator for 24 h, the cell culture supernatant was collected and the content of type I, III, and IV collagen was determined by the ELASA method, and the content increase rate was calculated (the blank control group was used as a basis for unified processing).

[0041] The results are as follows Figure 1-9 As shown. It can be seen that the single raw material showed an increasing trend in the test of type I, III, and IV collagen content compared with the blank group ( Figure 1-3 ), and when the three raw materials were combined in pairs, they showed greater efficacy than a single raw material. In particular, when algae oligosaccharides and carboxymethyl chitosan were present at the same time, the content of type I, III, and IV collagen showed a significantly higher increase than that of a single raw material, showing a significant synergistic combination effect, and when the three were added together, they showed a higher growth ratio ( Figure 4-9 ), in addition, whether in a combination of two or three, when controlling a single variable, when the amount of algae oligosaccharides and carboxymethyl chitosan added increased, the ratio increased, which shows that the efficacy of the combination increases with the increase in the amount of algae oligosaccharides and carboxymethyl chitosan added in the combination, showing a dose-dependent pattern.

[0042] The results of this example confirm that the combination of algal oligosaccharides, carboxymethyl deacetylated chitosan and hydrolyzed sodium hyaluronate can significantly promote the synthesis of type I, III and IV collagen in human skin fibroblasts, thereby having the effects of delaying aging, anti-wrinkle and firming, and can therefore be used in anti-aging products.

[0043] Embodiment 2:

[0044] An anti-aging skin care composition is prepared as follows: 10 parts by weight of algal oligosaccharides, 0.8 parts by weight of carboxymethyl chitosan and 5 parts by weight of hydrolyzed sodium hyaluronate are added in sequence to 82.5 parts by weight of deionized water, stirred to dissolve, then 1.2 parts by weight of carbomer-940 is added and stirred evenly, and finally 0.5 parts by weight of caprylyl glycol is added and stirred evenly to obtain the anti-aging skin care composition.

[0045] An anti-aging facial cream is prepared by adding the anti-aging skin care composition into a facial cream matrix, wherein the amount of the composition added into the facial cream is 1%.

[0046] The above-mentioned cream was tested for its anti-wrinkle efficacy with reference to T / ZHCA006-2019. In brief, 10 subjects (meeting the outer corner of the eye wrinkle rating of level 2 and above, age range 30-65 years old) were recruited, and the above-prepared cream was applied to the corners of the eyes and gently massaged until completely absorbed. The subjects used the test sample twice a day, once in the morning and once in the evening after cleansing, for 2 consecutive weeks. The average width of the outer corner of the eye wrinkles and the average width of the under-eye wrinkles were analyzed before use, after 1 week of use, and after 2 weeks of use (Antera 3D). The results of the average width analysis of the outer corner of the eye wrinkles are shown in Tables 1 and Fig.10 , 11 As shown, it can be found that after using the sample for 2 weeks, the average width of the outer corner wrinkles decreased by 39.23% compared with before use, and there was a significant difference (P < 0.05).

[0047] Table 1: Statistical results of the average width of outer corner eye wrinkles before and after using the samples (n=10)

[0048]

[0049] Note: ① Normality test P value>0.05, indicating that the data obeys normal distribution, paired t test is used. If it does not obey normal distribution, non-parametric rank sum test is used.

[0050] ② A significant P value of <0.05 indicates a significant difference compared with the results before use, indicated by "*"; a P value of <0.01 is indicated by "**"; a P value of <0.001 is indicated by "***". A significant P value of ≥0.05 indicates no significant difference compared with the results before use, indicated by "-".

[0051] The average width of wrinkles under the eyes is analyzed from Table 2 and Fig.12 , 13 It can be seen that after using the sample for 2 weeks, the average width of the wrinkles under the eyes decreased by 39.86% compared with before use, and there was a significant difference (P < 0.05).

[0052] Table 2: Statistical results of average width of under-eye wrinkles before and after using the samples (n=10)

[0053]

[0054] Note: ① Normality test P value>0.05, indicating that the data obeys normal distribution, paired t test is used. If it does not obey normal distribution, non-parametric rank sum test is used.

[0055] ② A significant P value of <0.05 indicates a significant difference compared with the results before use, indicated by "*"; a P value of <0.01 is indicated by "**"; a P value of <0.001 is indicated by "***". A significant P value of ≥0.05 indicates no significant difference compared with the results before use, indicated by "-".

[0056] in addition, Fig.14 This is an example of skin surface morphology analysis of a subject (Antera 3D). It can be seen that the subject's skin wrinkles have been significantly improved compared to before use.

[0057] Comparative Examples 1-3

[0058] The same method as in Example 1 was used to test the effects of 0.1% α-glucan oligosaccharide alone (Comparative Example 1), 0.1% α-glucan oligosaccharide + 0.008% carboxymethyl chitosan (Comparative Example 2), or 0.1% α-glucan oligosaccharide + 0.008% carboxymethyl chitosan + 0.05% hydrolyzed sodium hyaluronate (Comparative Example 3) on the production of type I collagen. The aforementioned concentrations were the final mass concentrations of the components in the culture medium for incubating cells. The results are shown in Table 1. Fig.15 shown.

[0059] Comparative Examples 4-6

[0060] The same method as in Example 1 was used to test the effects of 0.1% chitosan oligosaccharide alone (Comparative Example 4), 0.1% chitosan oligosaccharide + 0.008% carboxymethyl chitosan (Comparative Example 5), or 0.1% chitosan oligosaccharide + 0.008% carboxymethyl chitosan + 0.05% hydrolyzed sodium hyaluronate (Comparative Example 6) on the production of type I collagen. The above concentrations are the final mass concentrations of the components in the culture medium for incubating cells. The results are as follows: Fig.15 shown.

[0061] Comparative Examples 7-9

[0062] The same method as in Example 1 was used to test the effects of 0.1% trehalose alone (Comparative Example 7), 0.1% trehalose + 0.008% carboxymethyl chitosan (Comparative Example 8), or 0.1% trehalose + 0.008% carboxymethyl chitosan + 0.05% hydrolyzed sodium hyaluronate (Comparative Example 9) on the production of type I collagen. The aforementioned concentrations are the final mass concentrations of the components in the culture medium for incubating cells. The results are shown in Table 1. Fig.15 shown.

[0063] from Fig.15The results show that compared with the combination of algae oligosaccharides, carboxymethyl deacetylated chitosan and hydrolyzed sodium hyaluronate of the present invention, the other three combinations have poor effects on promoting the production of type I, III and IV collagen, and do not show obvious synergistic effects.

[0064] It should be noted that the preferred embodiments of the present invention are given in the specification and drawings of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described in this specification. These embodiments are not used as additional limitations on the content of the present invention. The purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. In addition, the above-mentioned technical features continue to be combined with each other to form various embodiments not listed above, which are all regarded as the scope of the present invention; further, for ordinary technicians in this field, they can be improved or transformed according to the above description, and all these improvements and transformations should belong to the protection scope of the claims attached to the present invention.

Claims

1. An anti-aging skin care composition prepared by using algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate, characterized in that: The anti-aging skin care composition comprises algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate.

2. The anti-aging skin care composition according to claim 1, characterized in that The weight proportions of algal oligosaccharides, carboxymethyl chitosan and hydrolyzed sodium hyaluronate in the anti-aging skin care composition are 6-10 parts, 0.3-0.8 parts and 5 parts respectively.

3. The anti-aging skin care composition according to claim 1, characterized in that: The anti-aging skin care composition further comprises excipients and preservatives.

4. The anti-aging skin care composition according to claim 3, characterized in that: The weight proportion of the preservative in the anti-aging skin care composition is 0.3-0.6 parts.

5. The anti-aging skin care composition according to claim 3, characterized in that: The preservative includes any one of 1,2-hexanediol, p-hydroxyacetophenone, caprylhydroxamic acid, caprylyl glycol, pentylene glycol and ethylhexylglycerin.

6. The anti-aging skin care composition according to claim 3, characterized in that: The auxiliary materials include deionized water and a thickener.

7. The anti-aging skin care composition according to claim 6, characterized in that: The weight proportion of the deionized water in the anti-aging skin care composition is 65-90 parts, and the weight proportion of the thickener in the anti-aging skin care composition is 1-1.5 parts.

8. The anti-aging skin care composition according to claim 6, characterized in that: The thickener includes any one of carbomer, xanthan gum, and ammonium acryloyldimethyltaurate / VP copolymer.

9. A process for preparing the anti-aging skin care composition according to any one of claims 6 to 8, characterized in that: The preparation process comprises the following steps: Weigh the raw materials according to the formula, add algal oligosaccharides, carboxymethyl deacetylated chitosan and hydrolyzed sodium hyaluronate into deionized water in sequence, stir to dissolve, then add the thickener and stir evenly, finally add the preservative and stir evenly to obtain the anti-aging skin care composition.

10. Use of the anti-aging skin care composition according to any one of claims 1 to 8 in the preparation of anti-aging cosmetics.

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