Food flora rapid detection device and method

Abstract

The invention discloses a food flora rapid detection device and method, and relates to the technical field of food detection.The food flora rapid detection device comprises a detection box body, a master controller and a display screen control panel which are used for controlling equipment are installed outside the detection box body, and a supporting column body is fixed between the top and the bottom of the detection box body; according to the food flora detection device, the reaction mechanism and the flora collector are arranged, so that the food flora detection operation is realized, meanwhile, the food flora detection device adopts an automatic technology, the operation is simple, the detection and analysis process of a sample can be quickly completed, the detection efficiency is greatly improved, a user can complete the detection process only by operating according to a prompt, and the detection efficiency is greatly improved. Two detection methods are adopted to carry out experiments on the flora at the same time, different detection methods are based on different principles and technologies, when similar results are obtained by the two methods, the accuracy of the detection results can be greatly enhanced, the risk of misjudgment caused by a single factor can be reduced by using the two methods, and the detection reliability is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of food detection, and in particular to a device and method for rapid detection of food flora. Background Art

[0002] Food flora refers to the various microbial communities present in food, which can include bacteria, fungi, yeast, etc. In order to ensure the safety and quality of food, food flora needs to be monitored and controlled, including the use of appropriate processing and storage methods to control the growth of microorganisms in food, and food flora testing to ensure that there are no pathogenic bacteria and harmful microorganisms in food. At the same time, it is also possible to develop healthier and more delicious foods by rationally utilizing beneficial flora.

[0003] The rapid detection device for food flora in the prior art requires manual use of the required device and instrument to perform a single test during the detection operation, resulting in low overall efficiency. After the test, the laboratory space is automatically disinfected manually, which reduces the work efficiency of the staff and makes the disinfection effect uncertain, resulting in poor disinfection effect.

[0004] In view of the above technical defects, a solution is now proposed. Summary of the invention

[0005] The purpose of the present invention is to use automated technology that is simple to operate and can quickly complete the detection and analysis process of samples, greatly improving the detection efficiency. Users only need to follow the prompts to complete the detection process. At the same time, two detection methods are used to experiment on the bacterial flora. Different experimental detection methods are based on different principles and technologies. When the two methods produce similar results, the accuracy of the bacterial flora detection results can be greatly enhanced.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a device and method for rapid detection of food flora, including a detection box, a main controller and a display control panel for controlling the equipment are installed on the outside of the detection box, a support column is fixed between the top and the bottom of the detection box, a plurality of evenly distributed fixed pillars are fixed on the outside of the support column, one end of each of the plurality of fixed pillars is connected to a movable track, a numerically controlled sliding seat a and a numerically controlled sliding seat b are installed inside the movable track, a flora collector is arranged inside the numerically controlled sliding seat a, a reaction mechanism is installed inside the numerically controlled sliding seat b, the reaction mechanism includes a solvent addition head and a solvent addition valve, one end of the solvent addition head is connected to an addition pipe , the solution addition valve is installed on the outside of the addition tube, a fixed disk is provided inside the detection box, a rotating disk is rotatably connected inside the fixed disk, a main motor for driving is installed at the bottom of the rotating disk, a plurality of symmetrically arranged mounting grooves are opened inside the rotating disk, a placement seat for placing the object to be detected is placed inside the mounting groove, a rotating gear disk is fixedly connected to the side wall of the bottom end of the rotating disk, a driving gear disk meshing with the rotating gear disk is installed inside the detection box, a rotating motor for driving is installed at the bottom end of the driving gear disk, a driving structure for adjusting the flora collector is installed inside the detection box, and a disinfection mechanism for disinfecting the detection box is installed inside the detection box.

[0007] Furthermore, the driving mechanism includes a driving seat, an anti-collision pad, a driving shaft, a driving motor and an auxiliary light. The driving flora collector is placed inside the driving seat, the anti-collision pad is located outside the driving seat and contacts the flora collector, the driving shaft is fixedly connected to the outer wall of the driving seat, the driving shaft is fixedly connected to one end of the driving motor, the auxiliary light is located on one side of the flora collector and fixedly connected to the outside of the driving seat, and the flora collector is located above the placement slot.

[0008] Furthermore, an adjustment mechanism is installed above the fixed plate, and the adjustment mechanism includes a sliding track, and the sliding track is internally slidably connected to two symmetrically arranged CNC moving seats, and a protective block is fixed to the top side wall of the CNC moving seat, and the bottom side wall of the CNC moving seat is hinged with a hinged rod, and connecting sleeves are installed at the ends of the two hinged rods, one end of the connecting sleeve is fixedly connected to a shaft body, and one end of the shaft body is connected to an electric push rod.

[0009] Furthermore, the protective mechanism includes a side wall of a fixed plate rotatably connected to a rotating rod, one end of the rotating rod is fixedly connected to a rotating motor, the outside of the rotating rod is rotatably connected to a protective cover, a sealing ring is installed on the outside of the protective cover, and a support sleeve frame is installed on the top side wall of the fixed plate, a groove for placing the protective cover is opened inside the support sleeve frame, and the support sleeve frame is located on one side of the placement seat.

[0010] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0011] The device and method for rapid detection of food flora realize the detection operation of food flora through the reaction mechanism and flora collector. At the same time, the food flora detection device adopts automation technology, which is simple to operate and can quickly complete the detection and analysis process of samples, greatly improving the detection efficiency. The user only needs to follow the prompts to complete the detection process. At the same time, two detection methods are used to experiment on the flora. Different detection methods are based on different principles and technologies. When the two methods produce similar results, the accuracy of the detection results can be greatly enhanced. The use of two methods can reduce the risk of misjudgment due to a single factor and improve the reliability of detection. When the device is used for detection, it can realize synchronous rapid detection of single and multiple samples. After the detection, the experimental space is automatically disinfected to ensure that the experimental container and the experimental space are sterilized to avoid contamination during detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 A schematic diagram of the internal structure of the detection box of the present invention is shown;

[0013] Figure 2 A schematic diagram of the structure of a local part of the rotating disk of the present invention is shown;

[0014] Figure 3 Another structural schematic diagram of the rotating disk of the present invention is shown;

[0015] Figure 4 A partial structural schematic diagram of the reaction mechanism of the present invention is shown;

[0016] Figure 5 The overall external structure schematic diagram of the present invention is shown;

[0017] Figure 6 The external structure schematic diagram of the method steps of the present invention is shown;

[0018] Figure 7 A schematic diagram of the control mechanism flow structure of the present invention is shown.

[0019] Legend: 1. Detection box; 2. Main controller; 3. Support column; 4. Moving track; 5. CNC sliding seat a; 6. CNC sliding seat b; 7. Bacteria collector; 8. Solvent adding head; 9. Adding tube; 10. Rotating disk; 11. Rotating gear disk; 12. Driving gear disk; 13. Rotating motor; 14. Driving seat; 15. Anti-collision pad; 16. Driving motor; 17. Sliding track; 18. Moving seat; 19. Protective block; 20. Articulated rod; 21. Connecting sleeve; 22. Electric push rod; 23. Rotating motor; 24. Protective cover; 25. Disinfection nozzle; 26. Liquid inlet pipe; 27. Automatic baffle; 28. Sealing ring; 29. ​​Purification circulator; 30. Main motor. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Embodiment 1:

[0022] like Figure 1-5 As shown, a device for rapid detection of food flora comprises a detection box 1, a main controller 2 and a display control panel for controlling the device are installed on the outside of the detection box 1, a support column 3 is fixed between the top and the bottom of the detection box 1, a plurality of evenly distributed fixed pillars are fixed on the outside of the support column 3, one end of each of the plurality of fixed pillars is connected to a movable track 4, a numerically controlled sliding seat a5 and a numerically controlled sliding seat b6 are installed inside the movable track 4, a flora collector 7 is arranged inside the numerically controlled sliding seat a5, a reaction mechanism is installed inside the numerically controlled sliding seat b6, the reaction mechanism comprises a solvent adding head 8, a solvent adding valve and a solvent controller, one end of the solvent adding head 8 is connected to an adding tube 9, the solution adding valve and the solvent controller are installed outside the adding tube 9, A fixed disk is provided inside the testing box 1, and a rotating disk 10 is rotatably connected inside the fixed disk. A main motor 30 for driving is installed at the bottom of the rotating disk 10, and a plurality of symmetrically arranged mounting grooves are provided inside the fixed disk. A placement seat for placing the object to be detected is placed inside the mounting groove. A rotating toothed disk 11 is fixedly connected to the bottom side wall of the rotating disk 10, and a driving toothed disk 12 meshing with the rotating toothed disk 11 is installed inside the testing box 1, and a rotating motor 13 for driving is installed at the bottom end of the driving toothed disk 12, and a driving motor 16 for driving is installed at the bottom end of the driving toothed disk 12, and a driving structure for adjusting the bacterial colony collector 7 is installed inside the testing box 1, and a disinfection mechanism for disinfecting the testing box 1 is installed inside the testing box 1.

[0023] The driving mechanism includes a driving seat 14, an anti-collision pad 15, a driving shaft, a driving motor 16 and an auxiliary light. The driving bacteria collector 7 is placed inside the driving seat 14, the anti-collision pad 15 is located outside the driving seat 14 and contacts the bacteria collector 7, the driving shaft is fixedly connected to the outer wall of the driving seat 14, the driving shaft is fixedly connected to one end of the driving motor 16, the auxiliary light is located on one side of the bacteria collector 7 and is fixedly connected to the outside of the driving seat 14, and the bacteria collector 7 is located above the placement slot;

[0024] The auxiliary lamp provides uniform illumination so that the colonies can be clearly seen in the image to adapt to different types of culture dishes and colony morphologies. The driving mechanism can move the bacterial colony collector 7 above the culture dish to scan the entire culture dish, thereby improving the accuracy and efficiency of the detection.

[0025] An adjustment mechanism is installed above the fixed plate, and the adjustment mechanism includes a sliding track 17. The sliding track 17 is internally slidably connected to two symmetrically arranged CNC moving seats 18. A protective block 19 is fixed to the top side wall of the CNC moving seat 18. A hinged rod 20 is hinged to the bottom side wall of the CNC moving seat 18. A connecting sleeve 21 is installed at the end of the two hinged rods 20. One end of the connecting sleeve 21 is fixedly connected to a shaft body, and one end of the shaft body is connected to an electric push rod 22.

[0026] The protection mechanism includes a side wall of a fixed plate rotatably connected to a rotating rod, one end of the rotating rod is fixedly connected to a rotating motor 23, the outside of the rotating rod is rotatably connected to a protective cover 24, a sealing ring is installed on the outside of the protective cover 24, and a support sleeve frame 25 is installed on the top side wall of the fixed plate. A groove for placing the protective cover 24 is opened inside the support sleeve frame 25, and the support sleeve frame 25 is located on one side of the placement seat.

[0027] The control mechanism includes an adjustment drive unit, an induction sensor unit, a detection sensor unit and a data sensor unit, and the adjustment drive unit, the induction sensor unit, the detection sensor unit and the data sensor unit are all signal-controlled by the master controller 2;

[0028] The inductive sensing unit is used to obtain the distance between the protective block 19 and the object to be detected through the distance measuring sensor, and to limit the minimum distance value between the two. When the limit condition is that the distance value between the protective block 19 and the object to be detected exceeds the minimum distance value, an adjustment instruction is generated and transmitted to the adjustment sensing unit;

[0029] The adjustment sensing unit is used to receive the adjustment instruction and drive the adjustment mechanism. When the adjustment instruction transmitted by the master controller 2 is received, the adjustment mechanism performs motion operation. During the operation of the adjustment mechanism, the distance sensor will continue to perform distance sensing. When the distance between the two reaches the minimum distance value, the sensing unit generates a stop instruction and transmits it to the adjustment sensing unit, and the adjustment sensing unit controls the adjustment mechanism to stop operating.

[0030] The detection sensor unit is used to control the movement of the bacterial colony collector 7 and the reaction mechanism. After receiving the adjustment work of the adjustment sensor unit, the main controller 2 controls the addition of solvent and the adjustment movement of the angle and viewing angle of the bacterial colony collector 7, and transmits the collected data information to the data sensor unit;

[0031] The data sensing unit is used to collect information data between devices and transmit the information data to the main controller 2 for analysis.

[0032] During the operation of the adjustment drive unit, the induction sensor unit, the detection sensor unit and the data sensor unit, information transmission and information sharing between the units can be realized. The main controller 2 is used to control the operation of the entire food flora detection device, including a display screen and operation buttons as well as the above-mentioned units, which is responsible for receiving and processing the data of the detection unit and controlling the work of each unit. The display screen is used to display the detection results and the operation interface, and the operation buttons are used to input operation instructions.

[0033] The disinfection mechanism includes an environmental monitor and a disinfection nozzle. Multiple disinfection nozzles are installed inside the detection box 1. A liquid inlet pipe 26 is installed at one end of the disinfection nozzle. A circulation port is opened on one side wall of the detection box 1. An automatic baffle 27 is installed on one side of the circulation port. A sealing ring 28 is installed on the outer wall of one side of the automatic baffle 27. A purification circulator 29 is installed at one end of the detection box 1.

[0034] The environment inside the detection box 1 is monitored by the disinfection mechanism, and the purpose of disinfecting the inside of the detection box 1 is achieved, so that the food flora is detected in a relatively safe and sterilized experimental environment, thereby improving the effect of food detection.

[0035] The environmental monitor is used to detect the internal environment of the detection box 1 to obtain detection data and determine whether to perform internal air purification circulation operation on the detection box 1. When air circulation is performed, when the environmental monitor receives an instruction, the automatic baffle 27 moves in a translational manner and the purification circulator 29 opens until the purification operation is completed.

[0036] When the above-mentioned structural components are used, the sample culture medium to be tested is placed inside the placement seat. When the culture medium needs to be limited, the electric push rod 22 will move and drive the connecting sleeve 21 to move through the shaft. When the connecting sleeve 21 moves, it will drive the hinged rod 20 to move. When the hinged rod 20 moves, it will drive the CNC moving seat 18 to move along the sliding direction of the sliding track 17 to achieve the positioning operation of the culture medium. After the culture medium is placed, the driving mechanism first adjusts the position of the bacterial colony collector 7, and the driving motor 16 drives the driving seat 14 to rotate, and collects and photographs the positions of the bacterial colony at different angles, and then adds the solution to the culture medium through the solvent adding head. After a period of reaction culture, the image is collected again, and the bacterial colony is analyzed through the collected image.

[0037] Embodiment 2:

[0038] like Figure 6-7 As shown, a method for using a device for rapid detection of food flora includes the following specific processes:

[0039] Step 1: Take samples of food under sterile conditions to obtain samples to be tested, perform preliminary pretreatment on the samples, including centrifugation and filtration, and place the pretreated samples in a specific culture medium as new test samples;

[0040] During the cultivation process, the above-mentioned culture medium mixes various ingredients in proportion according to a specific formula, and is prepared and dissolved. The prepared culture medium needs to be sterilized to kill possible bacteria and ensure the sterility of the culture medium. After selecting a suitable culture medium, the bacterial flora sample to be tested is inoculated into the prepared culture medium. The inoculated culture medium is placed under a suitable temperature and environment for cultivation. When the bacterial flora grows on the culture medium to a detectable concentration, the cultivation is stopped and then loaded into the food flora rapid detection device for detection.

[0041] Step 2: Place the sample to be tested added to the culture medium inside the detection box 1, and place it inside the placement seat according to the number of samples. After the placement is completed, adjust the single or multiple sample containers through the adjustment mechanism;

[0042] Step 3: The main controller 2 controls the solvent to be added to the reaction mechanism for operation, and the flora collector 7 is used to collect images of the sample to be tested, and then the sample to be tested is added through the solvent adding head, and then the added sample to be tested is experimentally cultured for a specific time. After the experimental culture is completed, the flora collector 7 is used to collect images of the sample to be tested again;

[0043] Step 4: According to the detection image results and reagent reaction conditions, analyze the biochemical characteristics of the sample and compare them with the bacterial species characteristics in the database. Based on the comparison results, give the bacterial species identification results.

[0044] For the above step 1, when receiving the sample, register the sample and record the source, type, and quantity of the sample. At the same time, prepare the reactants and experimental equipment for sample preparation, use biochemical reagents and ELISA solvents, and determine the type and quantity of the bacterial flora by detecting the biochemical reaction of the bacterial flora metabolites;

[0045] In the above step 2, high-precision motors and sensors are used to automatically and accurately move the object to be inspected according to the preset moving path and observation points.

[0046] For the above step 4, for the above step 3, when the flora collector 7 is used, the culture medium containing the object to be tested will be under the image acquisition head of the flora collector 7, and through the setting of the image acquisition head, when the image acquisition head is collecting, the flora collector 7 counts the colonies on the culture dish and records the results, and transmits the recorded results to the main controller 2, and queries through the control panel;

[0047] The bacterial colony collector 7 uses advanced digital cameras and optical lenses to obtain high-definition and high-color reproduction microbial images. The image acquisition device has automatic focus and automatic exposure functions to adapt to different samples and observation conditions;

[0048] For the above step 4, when analyzing the information data, a complete data management system is established to store, analyze and generate reports for the test data. Data traceability and sharing can be achieved, providing strong support for food safety supervision. Users have an intuitive and friendly human-computer interaction interface, and operators perform operations and settings. The interface provides real-time feedback based on the display of test progress and result information.

[0049] In the two embodiments provided in the present application, it should be understood that the disclosed devices and methods can be implemented in other ways; for example, the device embodiments described above are only schematic, for example, the division of modules is only a logical function division, and there may be other division methods in actual implementation, such as multiple modules or components can be combined or integrated into another system, or some features can be ignored or not executed; another point, the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, the indirect coupling or communication connection of devices or modules can be electrical, mechanical or other forms;

[0050] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A rapid detection device for food flora, comprising a detection box (1), the detection box (1) is externally provided with a master controller (2) for controlling the device and a display control panel, characterized in that: A support column (3) is fixed between the top and bottom of the detection box (1), and a plurality of evenly distributed fixed pillars are fixed to the outside of the support column (3), and one end of each of the plurality of fixed pillars is connected to a movable track (4), and a numerical control sliding seat a (5) and a numerical control sliding seat b (6) are installed inside the movable track (4), and a bacterial colony collector (7) is arranged inside the numerical control sliding seat a (5), and a reaction mechanism is installed inside the numerical control sliding seat b (6), and the reaction mechanism comprises a solvent addition head (8) and a solvent addition valve, and one end of the solvent addition head (8) is connected to an addition pipe (9), and the solution addition valve is installed outside the addition pipe (9).

2. The device for rapid detection of food flora according to claim 1, characterized in that: The detection box (1) is provided with a fixed disk inside, a rotating disk (10) is rotatably connected inside the fixed disk, a main motor (30) for driving is installed at the bottom of the rotating disk (10), a plurality of symmetrically arranged mounting grooves are provided inside the rotating disk (10), a placement seat for placing the object to be detected is placed inside the mounting groove, and a rotating gear disk (11) is fixedly connected to the side wall of the bottom end of the rotating disk (10).

3. The device for rapid detection of food flora according to claim 2, characterized in that: A driving toothed disc (12) meshing with the rotating toothed disc (11) is installed inside the detection box (1); a rotating motor (13) for driving is installed at the bottom end of the driving toothed disc (12); a driving structure for adjusting the bacterial colony collector (7) is installed inside the detection box (1); and a disinfection mechanism for disinfecting the detection box (1) is installed inside the detection box (1).

4. The device for rapid detection of food flora according to claim 3, characterized in that: The driving mechanism comprises a driving seat (14), an anti-collision pad (15), a driving shaft, a driving motor (16) and an auxiliary lamp; the bacterial colony collector (7) is placed inside the driving seat (14); and the anti-collision pad (15) is located outside the driving seat (14) and in contact with the bacterial colony collector (7).

5. The device for rapid detection of food flora according to claim 4, characterized in that: The driving shaft is fixedly connected to the outer wall of the driving seat (14), and the driving shaft is fixedly connected to one end of the driving motor (16). The auxiliary light is located on one side of the bacterial colony collector (7) and is fixedly connected to the outside of the driving seat (14). The bacterial colony collector (7) is located above the placement groove.

6. The device for rapid detection of food flora according to claim 5, characterized in that: An adjustment mechanism is installed above the fixed plate, and the adjustment mechanism comprises a sliding track (17). Two symmetrically arranged numerically controlled moving seats (18) are slidably connected inside the sliding track (17), and a protective block (19) is fixed to the top side wall of the numerically controlled moving seat (18).

7. The device for rapid detection of food flora according to claim 6, characterized in that: The bottom side wall of the numerically controlled movable seat (18) is hinged with an articulated rod (20), the ends of the two articulated rods (20) are provided with connecting sleeves (21), one end of the connecting sleeve (21) is fixedly connected to a shaft body, and one end of the shaft body is connected to an electric push rod (22).

8. The device for rapid detection of food flora according to claim 7, characterized in that: The protection mechanism comprises a side wall of a fixed disk rotatably connected to a rotating rod, one end of the rotating rod is fixedly connected to a rotating motor (23), and the outside of the rotating rod is rotatably connected to a protection cover (24).

9. The device for rapid detection of food flora according to claim 8, characterized in that: A sealing ring is installed on the outside of the protective cover (24), a supporting sleeve frame (25) is installed on the top side wall of the fixing plate, a slot for placing the protective cover (24) is opened inside the supporting sleeve frame (25), and the supporting sleeve frame (25) is located on one side of the placement seat.

10. The device for rapid detection of food flora according to claim 9, characterized in that: The disinfection mechanism comprises an environmental monitor and a disinfection nozzle. A plurality of the disinfection nozzles are installed inside a detection box (1). A liquid inlet pipe (26) is installed at one end of the disinfection nozzle. A circulation port is provided on a side wall of one side of the detection box (1). An automatic baffle (27) is installed on one side of the circulation port. A sealing ring (28) is installed on an outer wall of one side of the automatic baffle (27). A purification circulator (29) is installed at one end of the detection box (1).

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