Closed lentiviral vector culture device

Abstract

The utility model relates to the technical field of culture devices, in particular to a closed lentiviral vector culture device which comprises a box body, a sealing cover is connected to the top end of the box body through a hinge, a control panel is fixedly installed on the base face of the box body, telescopic rods are fixedly connected to the four corners of the bottom end of the sealing cover, and the telescopic rods are fixedly connected to the bottom end of the box body. A fixing plate is fixedly connected to one end of the telescopic rod, a sealing gasket is fixedly installed at the bottom end of the fixing plate, a water conveying pipe is fixedly connected to the top end of the inner wall of the water storage tank, a storage tank is fixedly installed at the right end of the box body, and a gas conveying opening is formed in the base face of the storage tank. According to the environment-friendly and energy-saving environment-friendly drying box, by arranging the box body, the sealing cover, the control panel, the telescopic rod, the sealing gasket, the sealing strip, the storage box, the external thread groove, the water pump, the exhaust fan, the connecting frame, the lapping ring, the hasp and the spring, the sealing effect is improved, and the practicability is enhanced.

Description

Technical Field

[0001] The utility model relates to the technical field of culture devices, in particular to a closed lentiviral vector culture device. Background Art

[0002] Lentiviral vectors can transduce non-replicating cells and also need to encode tumor cells. Cell culture experiments need to be carried out in a closed laboratory with a biosafety level 3. When culturing lentiviral vectors, a lentiviral vector culture device is required.

[0003] After searching, the published patent number CN220224164U mentioned a closed lentiviral vector culture device, including: a connecting shell, the top of which is hinged with a cover body; a placement structure, which is arranged inside the connecting shell; by setting the placement structure, it can be easily disassembled and installed by rotating the cover plate. After removing the cover plate, the inoculated culture dish can be placed in the placement groove for culture, and then the cover plate can be rotated and tightened. In this process, inert gas can be injected into the interior of the buffer airbag through the pressure ring, connecting rod, spring and extrusion ring, so that the gap between the culture dish and the placement groove can be filled under the action of the buffer airbag. The buffer airbag can increase the friction between the culture dish and the culture dish. When the device is hit, it can avoid the culture dish from colliding with the inner wall of the placement groove and being damaged. At the same time, the buffer airbag can reduce the impact of the collision on the culture dish, thereby improving the protection effect during the culture process.

[0004] Although the above solution solves the problems raised in the background technology, it still has the following shortcomings: when the culture dish inoculated with the virus vector is sealed, the sealing effect is poor, resulting in poor practicality. Utility Model Content

[0005] The purpose of the utility model is to provide a closed lentiviral vector culture device to solve the problems raised in the above background technology.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A closed lentiviral vector culture device comprises a box body, the top of the box body is connected to a sealing cover by a hinge, a control panel is fixedly installed on the base surface of the box body, four corners of the bottom end of the sealing cover are fixedly connected to telescopic rods, one end of the telescopic rod is fixedly connected to a fixing plate, a sealing gasket is fixedly installed on the bottom end of the fixing plate, a sealing strip is fixedly connected to the bottom end of the sealing cover close to the telescopic rod, a storage box is fixedly installed on the bottom end of the inner wall of the box body, external thread grooves are equidistantly provided on the outer side of the storage box, and the left side of the box body is fixedly connected to the bottom of the inner wall of the box body. A first delivery port is provided at the end, one end of the first delivery port is fixedly connected to a water tank through a through hole, a water pump is fixedly installed on the base surface of the water tank, a water inlet is provided on the side of the base surface of the water tank close to the water pump, a water pipe is fixedly connected to the top of the inner wall of the water tank, a storage box is fixedly installed on the right end of the box body, a second delivery port is provided on the side of the right end of the box body close to the storage box, one end of the second delivery port is fixedly connected to an exhaust fan, an air delivery port is provided on the base surface of the storage box, and an electric-controlled valve is fixedly connected to the left end of the water tank through a through hole.

[0008] As a preferred solution of the utility model, a connecting frame is fixedly connected to the inner side of the box body, rings are fixedly installed on both sides of the base surface of the box body close to the control panel through grooves, a buckle is fixedly installed on the base surface of the sealing cover through grooves, a handle is fixedly installed on the top of the sealing cover, and a groove compatible with the sealing strip is opened on the top of the box body.

[0009] As a preferred solution of the utility model, the control panel is electrically connected to the water pump, the exhaust fan, and the electrically controlled valve.

[0010] As a preferred solution of the utility model, a spring is provided on the outer side of the telescopic rod.

[0011] As a preferred solution of the utility model, the sealing gasket and the sealing strip are both made of rubber, the outer side of the external thread groove is connected to a protective cover through an internal thread groove, one end of the water inlet is fixedly connected to one end of the water pump, one end of the water pipe is fixedly connected to the other end of the water pump, the right end of the water tank is fixedly installed on the left end of the box body near the first delivery port, the left end of the water tank near the electric control valve is equidistantly provided with through holes, the outer side of the water pipe is equidistantly provided with through holes, and one end of the air delivery port is fixedly connected to one end of the exhaust fan.

[0012] As a preferred solution of the utility model, the number of the electrically controlled valve is one.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] In the utility model, a box body, a sealing cover, a control panel, a telescopic rod, a sealing pad, a sealing strip, a storage box, an external threaded groove, a water pump, an exhaust fan, a connecting frame, a ring, a buckle and a spring are arranged in a closed lentiviral vector culture device, so that a culture dish inoculated with a virus vector is placed inside the storage box. With the cooperation of the box body, the sealing cover, the control panel, the telescopic rod, the sealing pad, the sealing strip, the external threaded groove, the water pump, the exhaust fan, the connecting frame, the ring, the buckle and the spring, the sealing effect is improved and the practicability is enhanced. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 This is a schematic diagram of the overall internal structure of the utility model;

[0017] Figure 3 This is a schematic diagram of the structure of the water pump and water pipe of the utility model.

[0018] In the figure: 1. box body; 2. sealing cover; 3. control panel; 4. telescopic rod; 5. fixing plate; 6. sealing gasket; 7. sealing strip; 8. storage box; 9. external thread groove; 10. first delivery port; 11. water storage tank; 12. water pump; 13. water inlet; 14. water pipe; 15. storage box; 16. second delivery port; 17. exhaust fan; 18. air delivery port; 19. electric control valve; 20. connecting frame; 21. ring; 22. buckle; 23. handle; 24. spring; 25. protective cover. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0020] In order to facilitate the understanding of the utility model, the utility model will be described more comprehensively with reference to the relevant drawings below. Several embodiments of the utility model are given. However, the utility model can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the utility model more thorough and comprehensive.

[0021] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0023] For examples, see Figure 1-3 , the utility model provides a technical solution:

[0024] A closed lentiviral vector culture device comprises a box body 1, the top of the box body 1 is connected with a sealing cover 2 through a hinge, a control panel 3 is fixedly installed on the base surface of the box body 1, telescopic rods 4 are fixedly connected at the four corners of the bottom end of the sealing cover 2, one end of the telescopic rod 4 is fixedly connected with a fixing plate 5, a sealing gasket 6 is fixedly installed at the bottom end of the fixing plate 5, a sealing strip 7 is fixedly connected to the side of the bottom end of the sealing cover 2 close to the telescopic rod 4, a storage box 8 is fixedly installed at the bottom end of the inner wall of the box body 1, and external thread grooves 9 are equidistantly provided on the outer side of the storage box 8, a first delivery port 10 is provided at the left end of the box body 1, one end of the first delivery port 10 is fixedly connected with a water storage tank 11 through a through hole, a water pump 12 is fixedly installed on the base surface of the water storage tank 11, a water inlet 13 is provided on the side of the base surface of the water storage tank 11 close to the water pump 12, a water delivery pipe 14 is fixedly connected to the top of the inner wall of the water storage tank 11, and the box body A storage box 15 is fixedly installed on the right end of the main body 1. A second delivery port 16 is provided on the right end of the box body 1 near the storage box 15. One end of the second delivery port 16 is fixedly connected to an exhaust fan 17. The base surface of the storage box 15 is provided with an air delivery port 18, so that the culture dish inoculated with the virus vector is placed inside the storage box 8, and the protective cover 25 is rotated and fixed under the action of the external thread groove 9. The sealing cover 2 is flipped by the hinge to make the sealing gasket 6 press against the connecting frame 20, and the sealing strip 7 presses against the box body 1. The spring 24 is deformed by force to generate a rebound force to improve the sealing performance. At the same time, the box body 1 and the sealing cover 2 are fixedly connected under the cooperation of the ring 21 and the buckle 22. The spring 24 is limited by the telescopic rod 4 to improve the sealing effect. The culture environment is adjusted under the cooperation of the control panel 3, the water pump 12 and the exhaust fan 17, which enhances the practicality.

[0025] A connecting frame 20 is fixedly connected to the inner side of the box body 1, and a clasp 21 is fixedly installed on both sides of the base surface of the box body 1 near the control panel 3 through grooves, a buckle 22 is fixedly installed on the base surface of the sealing cover 2 through grooves, a handle 23 is fixedly installed on the top of the sealing cover 2, and a groove compatible with the sealing strip 7 is opened on the top of the box body 1. The control panel 3 and the water pump 12, the exhaust fan 17, and the electric control valve 19 are electrically connected. A spring 24 is provided on the outer side of the telescopic rod 4. The sealing gasket 6 and the sealing strip 7 are both made of rubber. A protective cover 25 is connected to the outer side of the external thread groove 9 through an internal thread groove. One end of the water inlet 13 is fixedly connected to one end of the water pump 12, and one end of the water delivery pipe 14 is fixedly connected to the other end of the water pump 12. The right end of the water storage tank 11 is fixedly installed on the side of the left end of the box body 1 close to the first delivery port 10, and the left end of the water storage tank 11 is close to the electric control valve 19. Through holes are equidistantly provided on the side, through holes are equidistantly provided on the outside of the water pipe 14, and one end of the air delivery port 18 is fixedly connected to one end of the exhaust fan 17, so that the culture dish inoculated with the virus vector is placed inside the storage box 8, and the protective cover 25 is rotated and fixed under the action of the external thread groove 9, and the sealing cover 2 is flipped over by the hinge, so that the sealing gasket 6 is pressed against the connecting frame 20, and the sealing strip 7 is pressed against the box body 1. The spring 24 is deformed by force to generate a rebound force to improve the sealing performance. At the same time, the box body 1 and the sealing cover 2 are fixedly connected with the cooperation of the ring 21 and the buckle 22, and the spring 24 is limited by the telescopic rod 4 to improve the sealing effect. After water is injected into the water storage tank 11, the water pump 12 is turned on through the control panel 3, and water is sprayed out under the action of the water pipe 14, and the exhaust fan 17 is turned on through the control panel 3 to adjust the culture environment, thereby enhancing practicality.

[0026] In this embodiment, please refer to Figure 1 The left end of the water storage tank 11 is fixedly connected to an electric control valve 19 through a through hole, so that the water inside the water storage tank 11 can be discharged through the electric control valve 19;

[0027] There is only one electric-controlled valve 19 , so the electric-controlled valve 19 can be opened through the control panel 3 to discharge the water inside the water storage tank 11 .

[0028] The working process of the utility model is as follows: when in use, check whether the appearance of the equipment is damaged. After the inspection is completed, power on the equipment, place the culture dish inoculated with the virus vector inside the storage box 8, rotate the protective cover 25, and fix it under the action of the external thread groove 9, flip the sealing cover 2 through the hinge, so that the sealing gasket 6 is pressed against the connecting frame 20, and the sealing strip 7 is pressed against the box body 1. The spring 24 is deformed by force to produce a rebound force to improve the sealing performance. At the same time, with the cooperation of the ring 21 and the buckle 22, the box body 1 and the sealing cover 2 are fixedly connected, and the spring 24 is limited by the telescopic rod 4 to improve the sealing effect. After water is injected into the water storage tank 11, the water pump 12 is turned on through the control panel 3, and the water is sprayed out under the action of the water pipe 14, and the exhaust fan 17 is turned on through the control panel 3 to adjust the culture environment. Among them, the electric control valve 19 is opened through the control panel 3 to facilitate the discharge of water inside the water storage tank 11, which enhances practicality and has promotion value.

[0029] The control panel 3, water pump 12, exhaust fan 17 and electric control valve 19 used in the present invention are all existing known electrical equipment and can be directly purchased and used on the market. Their structures, circuits, and control principles are all existing known technologies. Therefore, the structures, circuits, and control principles of the control panel 3, water pump 12, exhaust fan 17 and electric control valve 19 are not described in detail here.

[0030] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A closed lentiviral vector culture device, comprising a box body (1), characterized in that: The top of the box body (1) is connected to a sealing cover (2) via a hinge, a control panel (3) is fixedly mounted on the base surface of the box body (1), telescopic rods (4) are fixedly mounted at four corners of the bottom end of the sealing cover (2), one end of the telescopic rod (4) is fixedly mounted to a fixing plate (5), a sealing gasket (6) is fixedly mounted on the bottom end of the fixing plate (5), a sealing strip (7) is fixedly mounted on the bottom end of the sealing cover (2) near the telescopic rod (4), a storage box (8) is fixedly mounted on the bottom end of the inner wall of the box body (1), external thread grooves (9) are equidistantly formed on the outer side of the storage box (8), a first delivery port (10) is provided at the left end of the box body (1), and the first delivery port (10) is provided with a sealing strip (7) on the bottom end of the inner wall of the box body (1), and external thread grooves (9) are equidistantly formed on the outer side of the storage box (8), and a first delivery port (10) is provided at the left end of the box body (1). One end is fixedly connected to a water tank (11) through a through hole, a water pump (12) is fixedly mounted on the base surface of the water tank (11), a water inlet (13) is provided on the side of the base surface of the water tank (11) close to the water pump (12), a water delivery pipe (14) is fixedly connected to the top of the inner wall of the water tank (11), a storage box (15) is fixedly mounted on the right end of the box body (1), a second delivery port (16) is provided on the side of the right end of the box body (1) close to the storage box (15), one end of the second delivery port (16) is fixedly connected to an exhaust fan (17), an air delivery port (18) is provided on the base surface of the storage box (15), and an electric control valve (19) is fixedly connected to the left end of the water tank (11) through a through hole.

2. The closed lentiviral vector culture device according to claim 1, characterized in that: A connecting frame (20) is fixedly connected to the inner side of the box body (1), and a base surface of the box body (1) close to the control panel (3) is fixedly mounted with a buckle (21) via a groove on both sides thereof, and a base surface of the sealing cover (2) is fixedly mounted with a buckle (22) via a groove, and a handle (23) is fixedly mounted on the top of the sealing cover (2), and a groove matching the sealing strip (7) is provided at the top of the box body (1).

3. The closed lentiviral vector culture device according to claim 1, characterized in that: The control panel (3) is electrically connected to the water pump (12), the exhaust fan (17), and the electric control valve (19).

4. The closed lentiviral vector culture device according to claim 1, characterized in that: A spring (24) is provided on the outer side of the telescopic rod (4).

5. The closed lentiviral vector culture device according to claim 1, characterized in that: The sealing gasket (6) and the sealing strip (7) are both made of rubber. The outer side of the external thread groove (9) is connected to a protective cover (25) via an internal thread groove. One end of the water inlet (13) is fixedly connected to one end of the water pump (12). One end of the water delivery pipe (14) is fixedly connected to the other end of the water pump (12). The right end of the water storage tank (11) is fixedly mounted on the left end of the box body (1) near the first delivery port (10). The left end of the water storage tank (11) near the electric control valve (19) is provided with through holes at equal intervals. The outer side of the water delivery pipe (14) is provided with through holes at equal intervals. One end of the air delivery port (18) is fixedly connected to one end of the exhaust fan (17).

6. The closed lentiviral vector culture device according to claim 1, characterized in that: The number of the electrically controlled valve (19) is one.

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