Dual-mode chromatographic column preparation device

Abstract

The utility model discloses a dual-mode chromatographic column preparation device which comprises a rack, a lower shell, a power supply module, a storage battery, an electric push rod, a pressure sensor, a pressing cylinder, a shaping cylinder, an operating rod and a detection circuit, the lower end of the shaping cylinder is mounted on the lower shell, the front end of the shaping cylinder is provided with a movable door, and the upper part of the electric push rod is attracted to the front lower part of the rack through a magnet; the first set of pressure sensor is installed at the lower end of the electric push rod. A pressing plate is installed at the lower end of the first set of pressure sensor. The upper end of the inner side of the shaping cylinder is provided with internal threads, the outer side end of the pressing cylinder is provided with external threads, a rotating rod is installed outside the upper end of the pressing cylinder, the upper end of the second pressure sensor is installed at the lower end of the pressing cylinder, and a top plate is installed at the lower end of the second pressure sensor. The power supply module and the first set of detection circuit are arranged in the lower shell; and the storage battery and the second set of detection circuit are arranged in the element box. The pressing production mode is more flexible, the production efficiency is improved, and the density of the prepared finished product chromatographic column is prevented from being too large or too small.

Description

Technical Field

[0001] The utility model relates to the technical field of chromatographic image analyzer auxiliary equipment, in particular to a dual-mode chromatographic column preparation device. Background Art

[0002] High performance liquid chromatography analyzer is a device that uses gas chromatography theory to detect and analyze substances based on the classical chromatography analyzer. Technically, high performance liquid chromatography analyzer has higher detection sensitivity than ordinary chromatography analyzer and can continuously detect the effluent. Specifically, when the high performance liquid chromatography analyzer is working, its matching high pressure pump sucks the solvent in the liquid storage bottle and then outputs it. After the flow rate and pressure are measured, it is introduced into the injector. The test substance is injected by the injector and passes through the chromatographic column with the mobile phase (solvent). After separation on the column (chromatographic column), it enters the detector (the chromatographic column is located at the upper end of the detector). The signal of the effective component of the test substance detected by the detector is collected and processed by the data processing equipment matching the detector, and the chromatogram of the test substance is recorded, and then the composition of the test substance is obtained.

[0003] In the actual application of high-performance liquid chromatography analyzer, before each detection and analysis of the material components, a new chromatographic column (one-time use) needs to be placed in its detector. The chromatographic column is pressed by C18 filler, generally in a column shape. The chromatographic column structure can provide better separation effect and efficiency for the various components in the substance being tested, thereby ensuring the accuracy of the subsequent chromatogram of the substance being tested. In the prior art, for the pressing and molding of the chromatographic column, in the specific operation, the testing personnel manually put the C18 filler into the molding cavity, and then operate the hydraulic cylinder or electric pressing machine to press the filler into shape, and then manually remove the hydraulic cylinder or electric pressing mechanism, manually open the clamp at the front end of the molding cavity discharge port, and finally manually use tools to push out the finished chromatographic column through the discharge port. Although the above method meets the production needs to a certain extent, due to the limitations of structure and use function, there are still the following technical problems that need to be solved. First, when preparing chromatographic columns in batches, since both the hydraulic cylinder and the electric pressing mechanism require power to work, when the power is off on site due to various reasons, the preparation work cannot be carried out, which will have an adverse effect on production. Secondly, the compression density of the chromatographic column depends on the experience of the staff. In this way, since the staff cannot get specific data prompts during the production process, there is a probability that the compression density of the chromatographic column is too large or too small when the operation is improper. When the density of the chromatographic column is too large, the analyte injected by the injector is not easy to pass through the chromatographic column with the mobile phase, and the separation effect on the column is not good. The effective components entering the detector will be reduced, which will have an adverse effect on the detection and analysis of the relevant effective components in the analyte; on the contrary, if the density of the chromatographic column is too small, when the pressurized mobile phase and the analyte pass through the chromatographic column, there is a probability that the chromatographic column is loose and partially enters the detector due to excessive pressure, which will also have an adverse effect on the detector's analysis of the effective components in the analyte. In summary, it is particularly necessary to provide a chromatographic column preparation device that can compress the chromatographic column by electric and manual means and can indicate the compression density of the chromatographic column. Utility Model Content

[0004] In order to overcome the drawbacks of the existing chromatographic column pressing equipment used in conjunction with high performance liquid chromatographic analyzers due to structural limitations as described in the background, the utility model provides a dual-mode chromatographic column preparation device that, under the joint action of relevant mechanisms, allows workers to not only compress the chromatographic column electrically, but also prepare the chromatographic column manually. The production method is more flexible, and the production efficiency is correspondingly improved. After the compression degree of the chromatographic column reaches a set threshold, the worker can be prompted in time, effectively preventing the density of the prepared finished chromatographic column from being too large or too small, and providing effective technical support for the accurate detection of effective ingredients in the object being measured.

[0005] The technical solution adopted by the utility model to solve its technical problems is:

[0006] A dual-mode chromatographic column preparation device, comprising a frame, a lower shell, a power module, a battery, an electric push rod, a pressure sensor, a pressing cylinder, a shaping cylinder, an operating rod, and a support plate, characterized in that it also has a detection circuit; the lower shell is mounted on the support plate, the lower end of the shaping cylinder is mounted on the upper end of the lower shell, the front end of the shaping cylinder has an opening, a movable door is hingedly mounted on one side in front of the opening, and one side of the movable door is mounted together with the other side in front of the opening of the shaping cylinder through bolts; a magnet is mounted on the upper end of the electric push rod, and the electric push rod is attracted to the front upper end of the frame through the magnet; the pressure sensor and the detection circuit are respectively provided with at least two sets, the first set of pressure sensors is mounted on the lower end of the electric push rod, and the first set of pressure sensors is mounted on the lower end of the electric push rod. A pressure plate is installed at the lower end of the sensor; the inner upper end of the shaping cylinder has an internal thread, the outer end of the pressing cylinder has an external thread, a rotating rod is installed outside the upper end of the pressing cylinder, the rotating rod has an operating hole, the upper end of the second set of pressure sensors is installed at the lower end of the pressing cylinder, and a top plate is installed at the lower end of the second set of pressure sensors; the power module and the first set of detection circuits are installed in the lower shell, and the signal output end of the first set of pressure sensors is electrically connected to the signal input end of the first set of detection circuits; the battery and the second set of detection circuits are installed in the component box, the component box is installed outside the upper end of the pressing cylinder, and the signal output end of the second set of pressure sensors is electrically connected to the signal input end of the second set of detection circuits.

[0007] Furthermore, the outer diameter of the pressing plate is smaller than the inner diameter of the shaping cylinder, and the pressing plate and the shaping cylinder are in a vertical plane.

[0008] Furthermore, after the movable door is closed, its inner side and the inner side of the opening of the shaping cylinder are in the same plane.

[0009] Furthermore, the pressing cylinder is screwed into the internal thread of the shaping cylinder through the external thread, and the operating rod is movably sleeved in the operating hole at the upper end of the rotating rod.

[0010] Furthermore, the detection circuit includes an electrically connected adjustable resistor, a resistor, a transistor and a buzzer, one end of the adjustable resistor is connected to one end of the first resistor and one end of the second resistor, the other end of the second resistor is connected to the base of the transistor, the collector of the transistor is connected to the negative power input terminal of the buzzer, and the other end of the second resistor is connected to the emitter of the transistor.

[0011] Compared with the prior art, the utility model has the following beneficial effects: (1) In the utility model, the staff can not only compress the chromatographic column by electric means such as an electric push rod according to the needs, but also compress the chromatographic column manually through a pressing cylinder, so that the pressing production method is more flexible and the production efficiency is correspondingly improved; (2) Under the joint action of the pressure sensor and the detection circuit, whether the chromatographic column is compressed and prepared by electric or manual means, after the compression density of the chromatographic column reaches the set threshold, the staff can be promptly prompted, which effectively prevents the density of the prepared finished chromatographic column from being too large or too small, and provides effective technical support for the accurate detection of the effective components in the object being measured. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0013] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0014] Figure 2 It is a schematic diagram of the structure of a pressing cylinder of the utility model.

[0015] Figure 3 , 4 This is a circuit diagram of the utility model. DETAILED DESCRIPTION

[0016] Figure 1 , 2, 3, and 4, a dual-mode chromatographic column preparation device includes a "˥"-shaped frame 1, a lower shell 2, a power module W1, a battery G1 (24V / 1Ah), a power switch S1, an electric push rod M1, pressure sensors W2 and W3, a pressing cylinder 3, a shaping cylinder 4, an operating rod 5, a charging socket CZ, and a detection circuit 6; the hollow lower shell 2 is welded on a circular support plate 7, the shaping cylinder 4 with an open structure at the upper end is welded at the lower end to the middle part of the upper end of the lower shell 2, and an arc-shaped opening 41 is provided below the middle of the front end of the shaping cylinder 4, and the arc-shaped opening 41 is equipped with an arc-shaped The movable door 42, the upper and lower sides of the left side of the movable door 42 are hingedly installed at the left end of the opening 41, and a connecting seat 43 with a screw hole is welded to the outer side of the upper and lower ends of the right side of the movable door 42, and a locking manual bolt 44 is screwed into each connecting seat 43 through a thread; the upper and lower parts of the front end of the right side of the opening of the shaping cylinder are respectively provided with a screw hole 45, and two manual screws 44 of the connecting seat are screwed into the screw holes 45 of the upper and lower parts of the front end of the right side of the opening of the shaping cylinder, and the movable door 42 is sealed and installed at the front end of the opening 41 of the shaping cylinder; the upper end of the cylinder body of the electric push rod M1 is glued with a rectangular permanent The permanent magnet 8, the electric push rod M is vertically attracted to the lower part of the front upper end of the frame 1 (made of steel) through the magnet 8; the pressure sensor and the detection circuit are respectively provided with two sets, the first set of pressure sensor W2 is bolted to the lower end of the push column of the electric push rod M1, and a circular pressing plate 9 is bolted to the lower end of the force-bearing surface of the first set of pressure sensor W2; the inner upper end of the shaping cylinder 4 has an internal thread 46, the upper and lower ends of the pressing cylinder 3 are sealed structures, and the outer end has external threads 33 from top to bottom, and a rotating rod 31 is vertically welded to the outer middle of the upper end of the pressing cylinder 3, and an operating Hole 32, the upper end of the second set of pressure sensors W3 is bolted to the middle part of the lower end of the pressing cylinder 3, and its force-bearing surface is located at the lower part. The lower end of the second set of pressure sensors W3 is welded with a top plate 11 that is consistent with the outer diameter of the pressing cylinder; the power module W1, the power switch S1, and the first set of detection circuits 6 are installed on the circuit board in the lower shell 2, and the battery G1, the charging socket CZ and the second set of detection circuits 6 are installed in the component box 10, and the component box 10 is installed outside the upper end of the pressing cylinder 3 (there is an annular opening 101 in the middle of the component box 10, and the annular opening 101 is located at the lower end of the rotating rod 31).

[0017] Figure 1 , 2As shown in Figures 3 and 4, the outer diameter of the pressing plate 9 is slightly smaller than the inner diameter of the shaping cylinder 4 by 0.5 mm, and the pressing plate 9 and the shaping cylinder 4 are in a vertical plane. After the arc-shaped movable door 42 matching the arc-shaped opening 41 (larger than the radius of the prepared chromatographic column) is closed, its inner side is in the same plane as the inner side of the shaping cylinder opening 41. The pressing cylinder 3 is screwed into the inner thread of the shaping cylinder 4 through the outer thread, and the operating rod 32 is movably sleeved in the operating hole 32 at the upper end of the rotating rod 31. The first detection circuit includes an adjustable resistor RP1, resistors R1 and R2, a transistor Q1 and a buzzer B1 connected by circuit board wiring, one end of the adjustable resistor RP1 is connected to one end of the first resistor R1 and one end of the second resistor R2, the other end of the second resistor R2 is connected to the base of the transistor Q1, the collector of the transistor Q1 is connected to the negative power input end of the buzzer B1, and the other end of the second resistor R2 is connected to the emitter of the transistor Q1. The second detection circuit includes an adjustable resistor RP2, resistors R3 and R4, transistor Q2 and buzzer B2 connected by circuit board wiring, one end of the adjustable resistor RP2 is connected to one end of the first resistor R3 and one end of the second resistor R4, the other end of the second resistor R4 is connected to the base of transistor Q2, the collector of transistor Q2 is connected to the negative power input end of buzzer B2, and the other end of the second resistor R4 is connected to the emitter of transistor Q2. The power input ends 1 and 2 of the power module W1 are connected to the two poles of the AC 220V power supply through wires, the power output ends 3 and 4 of the power module W1 are connected to the power input ends 1 and 2 of the power switch S1, and through the power switch AS2 and the power input ends 1 and 2 of the first set of pressure sensor W2, the power input end of the first set of detection circuit, the positive power input end of the buzzer B1 and the emitter of the transistor Q1 are connected through wires. The power output terminals 3, 4 and 5, 6 of the power switch S1 are connected to the positive and negative and negative and positive and negative power input terminals of the electric push rod M1 respectively through wires, and the signal output terminal 3 of the first set of pressure sensor W2 is connected to the other end of the adjustable resistor RP1 at the signal input terminal of the first set of detection circuits through wires. The two poles of the battery G1 and the two ends of the charging socket CZ (when the battery G1 is out of power, the external power charger plug can be inserted into the charging socket CZ to charge the battery G1, and a charge can generally be used for more than half a year), and are connected in series with the power input terminals 1 and 2 of the second set of pressure sensor W3, the power input terminal of the second set of detection circuits, the positive power input terminal of the buzzer B2 and the emitter of the transistor Q2 through wires, and the signal output terminal 3 of the second set of pressure sensor W3 is connected to the other end of the adjustable resistor RP2 at the signal input terminal of the second set of detection circuits through wires.

[0018] Figure 1 , 2As shown in Figures 3 and 4, when the chromatographic column is prepared by electric means, the electric push rod M1 is attracted to the lower upper part of the front end of the frame through the magnet 8 at the upper end of the cylinder. After turning on the 220V power switch, the power module W1 is powered and its 3rd and 4th pins output a stable DC 24V DC power supply which enters the power switch S1 and the first set of pressure sensors and the power input end of the first set of detection circuits (assuming that the power switch AS1 is turned on). The staff puts the quantitative chromatographic column raw material to be pressed into the molding cylinder 4 through the upper end thereof (the lower end of the pressing plate 9 is now spaced a large distance from the upper end of the molding cylinder), and then the staff turns the power switch S1 handle to the left, and the 1, 2 feet and the 3, 4 feet of the power switch S1 are connected respectively, so that the positive and negative power input ends of the electric push rod M1 are energized, and its push column pushes the pressing plate 9 to gradually move downward along the molding cylinder 4 to gradually press the raw material into a column (turn off the power switch S1 after it is in place); after the subsequent pressing is completed, the staff turns the power switch S1 handle to the right, and the 1, 2 feet and the 5, 6 feet of the power switch S1 are connected respectively, so that the negative power input ends of the electric push rod M1 are energized, and its push column drives the pressing plate 9 to gradually move upward along the molding cylinder 4, until the lower end of the pressing plate 9 is spaced a large distance from the upper end of the molding cylinder 4, and the power switch S1 is turned off to prepare for pressing the next chromatographic column. When there is no power supply on site or the electric push rod M1 is damaged, the staff removes the electric push rod M1, and the staff puts the quantitative chromatographic column raw materials to be pressed into the upper end of the shaping cylinder 4 (the lower end of the pressing plate 9 is now spaced a large distance from the upper end of the shaping cylinder), and then the staff screws the pressing cylinder 3 into the shaping cylinder 4 through the thread, and when the staff sleeves the operating rod 32 into the operating hole at the upper end of the rotating rod 31 and rotates clockwise, the pressing cylinder 3 gradually moves down along the shaping cylinder 4, and the top plate 11 gradually presses the raw materials into a column (stop operating the operating rod after it is in place); after the subsequent pressing is completed, the staff rotates the operating rod 32 counterclockwise until the top plate at the lower end of the pressing cylinder 3 and the upper end of the shaping cylinder 4 are spaced a large distance apart, and stops operating to prepare for pressing the next chromatographic column. After the specific chromatographic column is pressed and formed, the staff opens the arc-shaped movable door 42, pries out the finished chromatographic column from the side end of the shaping cylinder opening with a flat-blade screwdriver, etc., and then closes the movable door again to prepare for the next preparation of the chromatographic column.

[0019] Figure 1 , 2As shown in Figures 3 and 4, when the chromatographic column is compressed electrically or manually, the force applied by the electric push rod M1 or the manual rotating rod 32 by the staff causes the force-bearing surface of the pressure sensor W2 or W3 to be subjected to different pressures, and its pin 3 will output a dynamically changing analog voltage signal. The higher the pressure, the greater the voltage signal, and vice versa. In the first detection circuit, when the pressure on the pressure sensor W2 is relatively small (the chromatographic column is relatively loosely compressed), the voltage signal output from its 3rd pin is divided by the adjustable resistor RP1 and the resistor R1, and the resistor R2 reduces the voltage and limits the current to enter the transistor Q1, which is lower than its 0.7V starting voltage. The transistor Q1 will not be turned on and the buzzer B1 will not be powered on to make a sound, indicating that the compression density of the chromatographic column has not reached the set value (the principle will be introduced later); when the pressure on the pressure sensor W2 is relatively large (the chromatographic column is relatively tightly compressed), the voltage signal output from its 3rd pin is divided by the adjustable resistor RP1 and the resistor R1, and the resistor R2 reduces the voltage and limits the current to enter the transistor Q1, which is higher than its 0.7V starting voltage. The transistor Q1 will be turned on and the collector output low level will enter the negative power input terminal of the buzzer B1, and the buzzer B1 will be powered on to make a sound, indicating that the compression density of the chromatographic column has reached the set value (the power switch S1 is turned off at this moment). In the second detection circuit, when the pressure on the pressure sensor W3 is relatively small (the chromatographic column is relatively loosely compressed), the voltage signal output from its 3rd pin is divided by the adjustable resistor RP2 and the resistor R3, and the resistor R4 steps down the voltage and limits the current to enter the transistor Q2, which is lower than its 0.7V starting voltage. The transistor Q2 will not be turned on and the buzzer B2 will not be powered on to make a sound, indicating that the compression density of the chromatographic column has not reached the set value (the principle will be introduced later); when the pressure on the pressure sensor W2 is relatively large (the chromatographic column is relatively tightly compressed), the voltage signal output from its 3rd pin is divided by the adjustable resistor RP2 and the resistor R3, and the resistor R4 steps down the voltage and limits the current to enter the transistor Q2, which is higher than its 0.7V starting voltage. The transistor Q2 will be turned on and the collector output low level will enter the negative power input terminal of the buzzer B2, and the buzzer B2 will be powered on to make a sound, indicating that the compression density of the chromatographic column has reached the set value (stop operating the rotating rod at this moment).

[0020] Figure 1 , 2 As shown in Figures 3 and 4, through the above, in the present invention, the staff can not only compress the prepared chromatographic column by electric means such as an electric push rod as needed, but also compress the chromatographic column manually through a pressing cylinder, and the pressing production method is more flexible, which improves the production efficiency accordingly; under the joint action of the pressure sensor and the detection circuit, no matter the chromatographic column is compressed and prepared by electric or manual means, after the compression degree of the chromatographic column reaches the set threshold, the staff can be promptly prompted, which effectively prevents the density of the prepared finished chromatographic column from being too large or too small, and provides effective technical support for the accurate detection of the effective ingredients in the object being measured. Figure 3 , 4Among them, the electric push rod M1 is a finished product of a reciprocating electric telescopic rod with a working voltage of DC 24V and a power of 50W; the power module W1 is a finished product of an AC 220V to DC 24V power module with an output power of 2KW; the pressure sensors W2 and W3 are finished products of pressure weighing sensors of model JHHM-H1, which have two power input terminals and a signal output terminal. The greater the detected pressure, the greater the voltage signal output by the signal output terminal, and vice versa; the transistors Q1 and Q2 are NPN transistors; the buzzers B1 and B2 are flashing buzzer alarms of model AD16-22SM; the resistance values ​​of resistors R1, R2, R3, and R4 are 10K, 47K, 10K, and 47K respectively; the power switches S1, S2, and S3 are toggle power switches, and their handles are respectively located at Outside the openings at the front end of the lower shell and the component box; the resistance values ​​of the adjustable resistors RP1 and RP2 are 470K (their adjustment handles are respectively located outside the other openings at the front end of the lower shell and the component box, and the lower shell and the component box on the side of the handle are marked with numbers representing the pressure. Before specific work, the staff adjusts the resistance values ​​of the adjustable resistors RP1 and RP2 to a larger value, the greater the voltage division between them and the resistors R1 and R3, and the transistor Q1 or Q2 will be turned on when the subsequent pressing pressure is relatively large, that is, the pressing force of the chromatographic column is set relatively large; the staff adjusts the resistance values ​​of the adjustable resistors RP1 and RP2 to a smaller value, the smaller the voltage division between them and the resistors R1 and R3, and the transistor Q1 or Q2 will be turned on when the subsequent pressing pressure is relatively small, that is, the pressing force of the chromatographic column is set relatively small).

[0021] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-limiting from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A dual-mode chromatographic column preparation device, comprising a frame, a lower housing, a power module, a battery, an electric push rod, a pressure sensor, a pressing cylinder, a shaping cylinder, an operating rod, and a support plate, characterized in that: It also has a detection circuit; the lower shell is installed on the support plate, the lower end of the shaping cylinder is installed on the upper end of the lower shell, the front end of the shaping cylinder has an opening, a movable door is hingedly installed on one side in front of the opening, and one side of the movable door is installed together with the other side of the opening of the shaping cylinder through bolts; a magnet is installed on the upper end of the electric push rod, and the electric push rod is attracted to the front upper end of the frame through the magnet; the pressure sensor and the detection circuit are respectively at least two sets, the first set of pressure sensors is installed on the lower end of the electric push rod, and the lower end of the first set of pressure sensors is installed with a pressing plate; the inner upper end of the shaping cylinder has an internal thread, and the outer end of the pressing cylinder is pressed The side end has an external thread, a rotating rod is installed on the outside of the upper end of the pressing cylinder, and the rotating rod has an operating hole. The upper end of the second set of pressure sensors is installed on the lower end of the pressing cylinder, and the lower end of the second set of pressure sensors is installed with a top plate; the power module and the first set of detection circuits are installed in the lower shell, and the signal output end of the first set of pressure sensors is electrically connected to the signal input end of the first set of detection circuits; the battery and the second set of detection circuits are installed in the component box, the component box is installed outside the upper end of the pressing cylinder, and the signal output end of the second set of pressure sensors is electrically connected to the signal input end of the second set of detection circuits.

2. A dual-mode chromatographic column preparation device according to claim 1, characterized in that: The outer diameter of the pressing plate is smaller than the inner diameter of the shaping cylinder, and the pressing plate and the shaping cylinder are in a vertical plane.

3. A dual-mode chromatographic column preparation device according to claim 1, characterized in that: After the movable door is closed, the inner side thereof and the inner side of the opening of the shaping cylinder are in the same plane.

4. A dual-mode chromatographic column preparation device according to claim 1, characterized in that: The pressing cylinder is screwed into the internal thread of the shaping cylinder through the external thread, and the operating rod is movably sleeved in the operating hole at the upper end of the rotating rod.

5. A dual-mode chromatographic column preparation device according to claim 1, characterized in that: The detection circuit includes an electrically connected adjustable resistor, a resistor, a transistor and a buzzer, one end of the adjustable resistor is connected to one end of the first resistor and one end of the second resistor, the other end of the second resistor is connected to the base of the transistor, the collector of the transistor is connected to the negative power input end of the buzzer, and the other end of the second resistor is connected to the emitter of the transistor.

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