Pretreatment separation device of micro-fluidic chip

Abstract

The utility model discloses a pretreatment separation device of microfluidic chip, including: separation box body, separation subassembly and fixed subassembly, the separation box body is provided with the inner cavity, the separation subassembly is installed in the inner cavity in cooperation, and the separation subassembly includes: rotary shaft, filter vat and filter plate, the filter plate is provided the bottom of filter vat, and the fixed subassembly is fixed on the rotary shaft. A filter membrane is attached to the inner wall of the filter barrel; a filter opening is formed in the bottom of the inner cavity, and a plurality of screens with different apertures are arranged on the filter opening; the fixing assembly comprises a supporting plate, a transmission motor and a rotating shaft. By arranging the filter plate and the filter membrane, large particles can be filtered out through tiny apertures, the purity of a sample is ensured, and by arranging the screens with different apertures on the left and right filter ports, the filter apertures can be adjusted according to needs, so that the filter efficiency is improved. The problem that cell clusters in filtered cell sap are different in size due to the fact that shaking force applied to different parts in the rotary filtering process is different is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic chips, in particular to a pretreatment separation device for a microfluidic chip. Background Art

[0002] A microfluidic chip is a miniaturized experimental platform that uses microfluidic technology to process and analyze samples in tiny flow channels and microscopic chemical reaction chambers. These chips are usually made of materials such as glass, silicon wafers or polymers, and have tiny channels, microstructures and functional areas on their surfaces. Before using microfluidic chips for cell analysis, it is usually necessary to filter and separate the cells in the sample because the tiny channels and microstructures of microfluidic chips are very sensitive to the size and morphology of cells. If there are large particles or cell clusters in the sample, they may clog the channels or affect the experimental results.

[0003] In the prior art, when the broken cells are filtered and separated, the sizes of cell clusters in the filtered cell fluid are different due to the different shaking forces applied to different parts during the rotary filtration process. When conducting microfluidic chip analysis and research, the larger part of the cell clusters will block the microchannels in the microfluidic chip, resulting in obstruction of fluid flow or even complete blockage of the channels, affecting the progress of the experiment. At the same time, since the size of the cell clusters is difficult to measure, the accuracy of the data will be affected to a certain extent in quantitative analysis and single-cell level research. Summary of the invention

[0004] The utility model overcomes the shortcomings of the prior art and provides a pretreatment separation device for a microfluidic chip.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the utility model is: a pretreatment separation device for a microfluidic chip, comprising: a separation box body, a separation component arranged inside the separation box body, and a fixing component installed outside the separation box body.

[0006] The separation box body is provided with an inner cavity, the separation assembly is installed in the inner cavity, and the separation assembly comprises: a rotating shaft, a filter barrel arranged at one end of the rotating shaft, and a filter plate arranged in cooperation with the filter barrel; the filter plate is arranged at the bottom of the filter barrel, and a filter membrane is fitted on the inner wall of the filter barrel;

[0007] A plurality of filter ports are arranged at the bottom of the inner cavity, the plurality of filter ports are divided into a left filter port and a right filter port, and a plurality of screens with different apertures are arranged on the left filter port and the right filter port;

[0008] The fixing assembly comprises: a plurality of support plates, a transmission motor arranged on each of the support plates, and a rotating shaft arranged at the output end of the transmission motor.

[0009] In a preferred embodiment of the utility model, one end of the rotating shaft passes through the supporting plate and is fixedly connected to the output end of the transmission motor, and one end of the rotating shaft away from the transmission motor is provided with an external thread.

[0010] In a preferred embodiment of the utility model, a plurality of sleeves are provided on the separation box body, an internal thread is provided in the sleeve, and one end of the rotating shaft provided with an external thread is sleeved in the sleeve, and the external thread on the rotating shaft corresponds to the internal thread in the sleeve.

[0011] In a preferred embodiment of the utility model, a plurality of the support plates are symmetrically installed on both sides of the separation box body, and a plurality of the sleeves are symmetrically arranged on both sides of the separation box body.

[0012] In a preferred embodiment of the utility model, a placement groove is provided at the bottom of the separation box body, a rotating motor is installed in the placement groove, and the output end of the rotating motor is fixedly connected to the rotating shaft.

[0013] In a preferred embodiment of the utility model, a cover plate is provided at the opening of the placement groove, a rotary bearing is provided on the cover plate, and the rotating shaft penetrates and connects with the rotary bearing.

[0014] In a preferred embodiment of the utility model, the rotary bearing is installed on the filter plate, and one end of the rotating shaft away from the rotating motor is sleeved on the rotary bearing.

[0015] In a preferred embodiment of the utility model, a plurality of conical discharge cavities are provided at the bottom of the separation box body, the filter port is arranged in cooperation with the conical discharge cavities, and a control valve is provided at one end of the conical discharge cavity where liquid flows out.

[0016] In a preferred embodiment of the utility model, a plurality of support rods are arranged on the two side walls outside the separation box body, and the two ends of the support rods are respectively fixedly connected to the separation box body and the support plate.

[0017] In a preferred embodiment of the utility model, a feed port is arranged on the top of the filter barrel, and a sealing cover is arranged on the feed port.

[0018] The utility model solves the defects existing in the background technology, and has the following beneficial effects:

[0019] (1) The utility model proposes a pretreatment separation device for a microfluidic chip. By setting a separation component, including a rotating shaft, a filter barrel and a filter plate, and setting a filter membrane in the filter barrel, cells or other particulate matter can be effectively separated. By setting the filter plate and the filter membrane, larger particles can be filtered out through tiny pores, thereby ensuring the purity of the sample. At the same time, by setting sieves with different pore sizes on the left and right filter ports, the filter pore size can be adjusted as needed, thereby solving the problem of different sizes of cell clusters in the filtered cell fluid obtained due to different shaking forces applied to different parts during the rotary filtration process, and solving the problem that larger parts of the cell clusters will block the microchannels in the microfluidic chip, resulting in obstruction of fluid flow and affecting the progress of the experiment, thereby meeting the processing requirements of different samples.

[0020] (2) The utility model ensures the stability and reliability of the device by setting up a fixed component, including a support plate and a transmission motor. At the same time, a rotating shaft is set so that the rotating shaft ensures the stability of the separation box body during rotation, thereby ensuring the stable rotation of the separation component. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0022] Figure 1 It is a three-dimensional structural diagram of a preferred embodiment of the utility model;

[0023] Figure 2 It is a front view of a preferred embodiment of the utility model;

[0024] Figure 3 It is a cross-sectional view of the internal structure of a preferred embodiment of the utility model;

[0025] In the figure: 1. separation box body; 2. transmission motor; 3. support plate; 4. control valve; 5. rotating shaft; 6. sleeve; 7. support rod; 8. inner cavity; 9. filter barrel; 10. sealing cover; 11. filter membrane; 12. filter plate; 13. rotating shaft; 14. rotating bearing; 15. screen; 16. conical discharge cavity. DETAILED DESCRIPTION

[0026] The present invention will now be further described in detail in conjunction with the accompanying drawings and embodiments. These drawings are simplified schematic diagrams that only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0027] like Figure 1 and Figure 2As shown, a pretreatment separation device for a microfluidic chip comprises: a separation box body 1, a separation component arranged inside the separation box body 1, and a fixing component installed outside the separation box body 1; an inner cavity 8 is arranged inside the separation box body 1, and the separation component is installed in the inner cavity 8, and the separation component comprises: a rotating shaft 13, a filter barrel 9 arranged on one end of the rotating shaft 13, and a filter plate 12 arranged in cooperation with the filter barrel 9; the filter plate 12 is arranged at the bottom of the filter barrel 9, and a filter membrane 11 is fitted on the inner wall of the filter barrel 9; a feed port is arranged at the top of the filter barrel 9, and a sealing cover 10 is arranged on the feed port.

[0028] One end of the rotating shaft 5 passes through the support plate 3 and is fixedly connected to the output end of the transmission motor 2, and an external thread is provided at the end of the rotating shaft 5 away from the transmission motor 2; a plurality of sleeves 6 are provided on the separation box body 1, and an internal thread is provided in the sleeve 6, and the end of the rotating shaft 5 with the external thread is sleeved in the sleeve 6, and the external thread on the rotating shaft 5 corresponds to the internal thread in the sleeve 6.

[0029] It should be noted that the plurality of transmission motors 2 are driven synchronously, and when the separation box body 1 is clamped, the rotating motor drives the rotating shaft 5 to rotate in the sleeve 6 .

[0030] like Figure 3 As shown, a plurality of filter ports are arranged at the bottom of the inner cavity 8, and the plurality of filter ports are divided into a left filter port and a right filter port, and a plurality of screens 15 with different apertures are arranged on the left filter port and the right filter port; the fixed component includes: a plurality of support plates 3, a transmission motor 2 arranged on each support plate 3, and a rotating shaft 5 arranged at the output end of the transmission motor 2.

[0031] By setting a separation component, including a rotating shaft 13, a filter barrel 9 and a filter plate 12, and setting a filter membrane 11 in the filter barrel 9, cells or other particles can be effectively separated. By setting the filter plate 12 and the filter membrane 11, it is ensured that larger particles are filtered out through a tiny aperture, thereby ensuring the purity of the sample. At the same time, by setting screens 15 with different apertures on the left and right filter ports, the filter aperture can be adjusted as needed.

[0032] A plurality of support plates 3 are symmetrically installed on both sides of the separation box body 1, and a plurality of sleeves 6 are symmetrically arranged on both sides of the separation box body 1; a placement groove is arranged at the bottom of the separation box body 1, and a rotating motor is installed in the placement groove, and the output end of the rotating motor is fixedly connected to the rotating shaft 13.

[0033] A cover plate is provided at the opening of the placement slot, on which a rotating shaft 5 bearing is provided and a rotating shaft 13 penetrates and connects the rotating shaft 5 bearing; a rotating shaft 5 bearing is installed on the filter plate 12, and one end of the rotating shaft 13 away from the rotating motor is sleeved on the rotating shaft 5 bearing.

[0034] A plurality of conical discharge cavities 16 are provided at the bottom of the separation box body 1, the filter port is matched with the conical discharge cavity 16, and a control valve 4 is provided at one end of the conical discharge cavity 16 from which the liquid flows out; a plurality of support rods 7 are provided on the two side walls outside the separation box body 1, and the two ends of the support rods 7 are fixedly connected to the separation box body 1 and the support plate 3 respectively.

[0035] By setting a fixed component, including a support plate 3 and a transmission motor 2, the stability and reliability of the device are ensured. At the same time, the rotating shaft 5 is set so that the rotating shaft 13 can ensure the stability of the separation box body 1 when rotating, thereby ensuring the stable rotation of the separation component.

[0036] When the utility model is used, the sealing cover 10 plate on the top of the separation box body 1 is opened, and the cell fluid or sample containing particulate matter to be processed is loaded into the separation box body 1 through the feed port on the top of the filter barrel 9. After waiting for the loading to be completed, the feed port and the sealing cover 10 plate on the upper part of the separation box body 1 are closed, the transmission motor 2 is started, the separation box body 1 is clamped, and then the rotating motor is started, the rotating shaft 13 is rotated to filter the cell fluid sample, and the rotating shaft 13 drives the filter barrel 9 and the filter plate 12 to rotate together, and the particulate matter in the sample is intercepted by the filter membrane 11, and the purified sample enters the filter barrel 9 through the filter membrane 11;

[0037] The sample in the filter barrel 9 passes through the filter membrane 11, and the smaller particles pass through the filter membrane 11, while the larger particles are intercepted on the filter membrane 11; it is filtered again through the filter plate 12, and the filtered cell fluid flows into the filter port provided with the screen 15 with different apertures, and the cell fluid is separated and discharged through the left filter port and the right filter port. The conical discharge cavity 16 at the bottom of the separation box body 1 collects the sample, and the outflow speed and direction of the liquid are controlled by the control valve 4. After the above steps are completed, the operation of the rotating shaft 13 is stopped, and the subsequent operation is waited for.

[0038] The above is based on the ideal embodiment of the utility model. Through the above description, relevant personnel can make various changes and modifications without deviating from the technical concept of the utility model. The technical scope of this utility model is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. A pretreatment separation device for a microfluidic chip, comprising: A separation box body (1), a separation component arranged inside the separation box body (1), and a fixing component installed outside the separation box body (1), characterized in that: The separation box body (1) is provided with an inner cavity (8), the separation assembly is mounted in the inner cavity (8), and the separation assembly comprises: a rotating shaft (13), a filter barrel (9) arranged at one end of the rotating shaft (13), and a filter plate (12) arranged in cooperation with the filter barrel (9); the filter plate (12) is arranged at the bottom of the filter barrel (9), and a filter membrane (11) is fitted on the inner wall of the filter barrel (9); The bottom of the inner cavity (8) is provided with a plurality of filter ports, the plurality of filter ports are divided into a left filter port and a right filter port, and the left filter port and the right filter port are provided with a plurality of screens (15) with different apertures; The fixing assembly comprises: a plurality of support plates (3), a transmission motor (2) arranged on each of the support plates (3), and a rotating shaft (5) arranged at the output end of the transmission motor (2).

2. The pretreatment separation device of a microfluidic chip according to claim 1, characterized in that: One end of the rotating shaft (5) passes through the supporting plate (3) and is fixedly connected to the output end of the transmission motor (2); an end of the rotating shaft (5) away from the transmission motor (2) is provided with an external thread.

3. The pretreatment separation device of a microfluidic chip according to claim 2, characterized in that: A plurality of sleeves (6) are provided on the separation box body (1), and an internal thread is provided in the sleeve (6). One end of the rotating shaft (5) provided with an external thread is sleeved in the sleeve (6), and the external thread on the rotating shaft (5) corresponds to the internal thread in the sleeve (6).

4. The pretreatment separation device of a microfluidic chip according to claim 3, characterized in that: A plurality of the support plates (3) are symmetrically mounted on both sides of the separation box body (1), and a plurality of the sleeves (6) are symmetrically arranged on both sides of the separation box body (1).

5. The pretreatment separation device of a microfluidic chip according to claim 1, characterized in that: The bottom of the separation box body (1) is provided with a placement groove, a rotating motor is installed in the placement groove, and the output end of the rotating motor is fixedly connected to the rotating shaft (13).

6. The pretreatment separation device of a microfluidic chip according to claim 5, characterized in that: A cover plate is provided at the opening of the placement groove, a rotating shaft (5) bearing is provided on the cover plate, and the rotating shaft (13) passes through and is connected to the rotating shaft (5) bearing.

7. The pretreatment separation device of a microfluidic chip according to claim 6, characterized in that: The filter plate (12) is mounted with the rotating shaft (5) bearing, and one end of the rotating shaft (13) away from the rotating motor is sleeved on the rotating shaft (5) bearing.

8. The pretreatment separation device of a microfluidic chip according to claim 1, characterized in that: A plurality of conical discharge cavities (16) are provided at the bottom of the separation box body (1), the filter port is arranged in cooperation with the conical discharge cavities (16), and a control valve (4) is provided at one end of the conical discharge cavity (16) where liquid flows out.

9. The pretreatment separation device of a microfluidic chip according to claim 1, characterized in that: A plurality of support rods (7) are arranged on the two side walls outside the separation box body (1), and the two ends of the support rods (7) are respectively fixedly connected to the separation box body (1) and the support plate (3).

10. The pretreatment separation device of a microfluidic chip according to claim 1, characterized in that: The top of the filter barrel (9) is provided with a feed inlet, and a sealing cover (10) is provided on the feed inlet.

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