Holographic fluctuation microscopy apparatus and method for determining mobility of particle and/or cell dispersions

US20130260396A1Inactive Publication Date: 2013-10-03ARRYX INC
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
Publication Date
2013-10-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to an instrument and a measurement apparatus and methodology that yields a measurement and test methodology that characterizes a population of cells / particles or detects a sub-population of cells / particles based on their detected mobility in a quick and efficient manner.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] The present invention claims priority from U.S. Provisional Patent Application No. 61 / 347,956, filed May 25, 2010, and U.S. Provisional Patent Application No. 61 / 348,072, filed May 25, 2010, the contents of both of which are herein incorporated by reference in their entirety.BACKGROUND OF THE INVENTION

[0002] 1. Field of the Invention

[0003] The present invention relates to an instrument and a measurement apparatus and methodology that yields a measurement and test method that characterizes a population of cells / particles and / or their interactions with a chemically modified (or unmodified) surface, and / or detects a sub-population of cells / particles based on their detected mobility in a quick and efficient manner.

[0004] 2. Description of the Related Art

[0005] Traditional cell / particle tracking methods may utilize standard microscopy techniques such as brightfield, darkfield, and fluorescence to detect particles moving in two or three dimensions....

Examples

Embodiment Construction

[0091]The present invention relates to an instrument and measurement apparatus and methodology used for measurements and testing, that characterize a population of cells / particles or which can detect a sub-population of cells / particles, based on their detected mobility, in a quick and efficient manner.

[0092]Apparatus

[0093]In one exemplary embodiment, FIG. 1A shows a schematic diagram of an in-line holographic microscope apparatus 10 designed as the means to measure the mobility of particles (i.e., cells) in order to infer surface interactions (i.e., presence or absence of specific surface—particle interactions), and / or collective diffusion / visco-elastic properties of the particle dispersion (e.g., effective viscosity).

[0094]In the exemplary embodiment of FIGS. 1A and 1C, the apparatus 10 used for carrying out the present invention includes a coherent light source (e.g., laser, superluminescent diode) 100 that is collimated by a collimator 101, which may be coupled to an optical-fibe...