36 results about "Spermatozoa Capacitation" patented technology

This invention describes unique patterns of distribution of ganglioside GM1 in non-capacitated sperm and demonstrates that the pattern of distribution of GM1 undergoes changes that can be correlated with the process of capacitation and / or with acrosomal exocytosis. Accordingly, the present invention discloses a method for determining the ability of sperm to respond to capacitation and / or acrosomal exocytosis stimuli. The method comprises determination of distribution pattern for GM1. The method can be used for both diagnostic and predictive purposes when assessing male reproductive fitness, and can also be used to assess the effects on sperm of cryoprotective agents and protocols, and contraceptive agents.

This disclosure provides a method for determining male fertility status, and its relationship to the probability of generating a pregnancy as calculated using a regression model. The method comprises determining GM1 localization patterns following induced sperm capacitation, identifying the percentage of various patterns, particularly the ratio of [(AA+APM) / total number of GM1 localization patterns] and determining if the percentage of certain GMI localization patterns in response to induced capacitation is altered. Based on the change in the percentage of localization patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.

The invention provides hydrogen ion enriched acidic conservation contraception gel for department of gynecology and a preparation method of the hydrogen ion enriched acidic conservation contraceptiongel, and relates to the technical field of medical health. The preparation method comprises the steps of performing wetting and dispersing on an ammonium acryloyldimethyltaurate / VP copolymer with glycerin or propylene glycol to form paste, mixing the paste with a saturated hydrogen-enriched aqueous solution, and performing standing until the ammonium acryloyldimethyltaurate / VP copolymer presents expansional state, so as to obtain a glue solution; mixing lactic acid with glycollicacid, citric acid, lactein, calcium propionate, ferment, active VC, glutathione GSH and the saturated hydrogen-enriched aqueous solution to obtain a mixed solution; and then uniformly mixing the mixed solution with the glue solution, and adjusting the pH value to 3.8-4.0 so as to obtain the hydrogen ion enriched acidic conservation contraception gel. The hydrogen ion enriched acidic conservation contraception gel disclosed by the invention does not destroy beneficial bacteria in the vagina, and does not have any side effects; the pH value of products is completely consistent with the normal pH value of the vagina; the vagina mucosa can be urged to be young, and the disease resistance can be improved; and the hydrogen ion enriched acidic conservation contraception gel can restrain breeding of pathogenic bacteria, prevent vagina infection, restrain capacitation and sperm activity, and exert the contraception effects.

The invention discloses a method for in vitro capacitation of yak sperms. The method mainly comprises a method for unfreezing yak frozen semen, sperm in vitro capacitation liquid, a method for capacitation, sperm in vitro fertilization liquid and a method for evaluating the capacitation effect. By adopting the method, a specific condition for in vitro capacitation of the yak sperms is provided to ensure the quality and efficiency of the in vitro capacitation of the yak sperms. The method is comprehensive in technology and method, convenient to operate and accurate in determination result, can be completely used for in vitro capacitation of the yak sperms or in vitro production of embryos, and can effectively improve the breeding efficiency of yaks.

The invention discloses a test reagent for sperm acrosome reaction, which is characterized in that it includes sperm capacitation treatment solution, acrosome reaction induction solution and staining solution; wherein, the sperm capacitation treatment solution contains Lactobacillus casei LC-N235 fermented supernatant. The invention also provides a kit made with the sperm acrosome reaction reagent. The invention also provides a method for detecting the acrosome by using the kit made of the sperm acrosome reaction reagent. The advantages of the present invention: (1) Breakthrough shortens the time required for sperm capacitation and improves efficiency. (2) Protect the sperm and improve the vitality of the sperm so as to reduce the deviation caused by improper preservation of the sample.

Belonging to the technical field of livestock breeding, the invention discloses a bovine sperm capacitation solution and an in vitro sperm capacitation method. The bovine sperm capacitation solution mainly consists of the following components: 6.7500mg / mL NaCl, 0.3000mg / mL KCl, 2.5000mg / mL NaHCO3, 0.0620mg / mL NaH2PO4.2H2O, 0.2960mg / mL CaCl2.2H2O, 0.1000mg / mL MgCl2.6H2O, 0.1120mg / mL sodium pyruvate, 0.0186mg / mL EDTA-Na, 0.0100mg / mL phenol red, 6.0000mg / mL BSA, 1.0000mg / mL glucose, 0.4800mg / mL caffeine, 0.0500mg / mL heparin, and 0.025-0.05mg / mL Nisin. The capacitation solution provided by the invention can effectively ensure the sperm motility, sperm plasma membrane integrity rate, acrosomal integrity rate and mitochondrial activity, and maintains the in vitro sperm capacitation effect.

The invention relates to a bovine sperm treatment method and a bovine intracytoplasmic sperm injection method, and belongs to the technical field of intracytoplasmic sperm injection. According to the bovine sperm treatment method, firstly, the sperm is treated by using a weakly alkaline solution, and therefore, the tail part of the sperm is shortened by 1 / 3-1 / 2; and then, the sperm of which the tail part is shortened by 1 / 3-1 / 2 is selected and is injected into the oocytes. After the bovine sperm treatment method disclosed by the invention is adopted for carrying out the treatment, only the tail part of the sperm is shortened by 1 / 3-1 / 2, the sperm still retains the centrosome, the integrity of the sperm is structurally retained, the method is more similar to the change of sperm capacitation in a natural fertilization process, the embryonic development potential is improved, and the development rate of microinjection embryos is improved. In addition, the tail part of the sperm treated by the bovine sperm treatment method is shortened by 1 / 3-1 / 2, and shortening of the tail part can lower the difficulty of microinjection, improve the operation speed of injection and improve the efficiency of intracytoplasmic sperm injection.

This disclosure provides a method for determining male fertility status. The method comprises determining GM1 localization patterns following induced sperm capacitation, identifying the percentage of various patterns, particularly the ratio of [(AA+APM) / total number of GM1 localization patterns] and determining if the percentage of certain GM1 localization patterns in response to induced capacitation is altered. Based on the change in the percentage of localization patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.

Provided are a kit for evaluating the sperm quality after in vitro capacitation and a method of use thereof. The kit comprises a detection buffer 1, a BWW culture solution, a solution for in vitro capacitation, a fluorescent reagent, a sialidase standard stock solution and a detection buffer 2. The detection buffer 1 is a phosphate buffer or a BWW culture solution. The detection buffer 2 is a phosphate buffer containing 0.05 mmol / l of sodium acetate and 0.25 μg / μl of bovine serum albumin.

An extracellular matrix protein, such as fibronectin, vitronectin or laminin, is added to a sperm sample to bring the sperm into a low motility, non-capacitated state or maintain a pre-existing non-capacitated state. Subsequently, at an appropriate time in an in vitro fertilization study or in vivo fertilization procedure, angiotensin II or a related peptide is added to the sperm sample to enhance motility and capacitate the sperm. The extracellular matrix protein prevents undesirable early natural capacitation, and artificial insemination procedures can be made more precise, as sperm can be brought to the right state of capacitation for transfer at precisely the right time. The treatment with angiotensin II can be combined with use of the peptide RGD to compete with the binding of the extracellular matrix protein and so suppress its effect.

The invention provides a method for improving the sperm motility and increasing an in-vitro-fertilization rate of frozen bovine semen by the aid of genistein. The method is particularly characterized in that the genistein containing 1%o of DMSO (dimethyl sulfoxide) is added into SP-TALP (serum phosphate-total alkaline phosphatase) washing liquid for washing the frozen bovine semen in earlier-stage processing for the frozen bovine semen, or mixed liquid of PHE (phenylalanine) capacitation liquid and IVF-TALP (in vitro fertilization-total alkaline phosphatase) fertilization liquid containing bovine semen in a capacitation processing stage of second-stage pre-fertilization semen or prepared fertilization drops containing oocytes in a third-stage in-vitro-fertilization procedure; the added amounts of the genistein in two earlier stages of a fertilization procedure of each bottle of frozen bovine semen are 10 micromoles per liter, and the final concentration of the genistein in mixed liquid of the bovine semen and the fertilization drops containing the oocytes is 10 micromoles per liter when the genistein is added in a third stage of the fertilization procedure of each bottle of frozen bovine semen. The method has the advantages that the genistein is respectively used in the three in-vitro-fertilization stages, so that the sperm motility is greatly improved, and the sperm fertilization rate is greatly increased.

The invention provides a method for improving the moveability of sperm in frozen bull semen and the in vitro fertilization rate. The method is characterized in that when SP-TALP washing liquid is used for washing at a prior disposal stage of the frozen bull semen, or when PHE (phenylalanine) capacitation liquid is used for capacitation disposal at a capacitation disposal stage of the semen before fertilization, or when fertilization drips are prepared at an in vitro fertilization stage, glutathione and caffeine of which the purities are both higher than 98% are added; in the fertilization process of each piece of frozen bull semen, in the SP-TALP washing liquid containing bull semen or in the mixed liquid of IVF-TALP seminal fluid containing the bull semen and the PHE capacitation liquid or in the mixed liquid of the fertilization drips containing oocytes and the bull semen, the final concentration of the glutathione and the caffeine is 5mmol / L. According to the method disclosed by the invention, two substances are jointed to be respectively applied to three stages in the fertilization process of the frozen bull semen, the moveability of the sperm is greatly improved, and the fertilization rate of the sperm is improved.

The invention discloses application of EMC10 protein as a biomarker in diagnosis of male infertility. An experiment proves that by adopting substances capable of increasing EMC10 protein activity and / or expression quantity, the male infertility can be prevented, the male infertility can be treated, fertilisation of sperm and eggs is promoted, fertilisation of sperm and zona pellucida-free eggs ispromoted, the mixing ability of the sperm and a cell membrane is improved, sperm dysfunction is treated, sperm maturation is promoted, maturation of the sperm in epididymis is promoted, the motion ability of the sperm is improved, a protein tyrosine phosphorylation level related with capacitation of the sperm is improved, the capacitation of the sperm is promoted, the acrosome reaction ability ofthe sperm is improved, an abnormal form of the sperm is repaired, the activity of sodium / potassium-APT enzyme is recovered, ionic balance in the sperm is maintained, Na<+> balance in the sperm is maintained, HCO3-balance in the sperm is maintained, the balance of a pH value in the sperm is maintained, and the fertility of males is improved. The EMC10 protein disclosed by the invention has an important application value.

Processing and use of fluids from the reproductive tract (biofluids) to improve the in vitro production of mammalian embryos comprising the following steps: a) fractionation and processing of biofluids through a sorting, purification, lyophilization and subsequent storage; b) a method of sperm capacitation in a culture medium supplemented with biofluids; c) in vitro fertilization in a medium enriched with biofluids and d) subsequent in vitro culture with development of the obtained embryos to any stage of preimplantational development in culture media supplemented with biofluids.