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38 results about "Spermatozoa Capacitation" patented technology
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Capacitation is the penultimate step in the maturation of mammalian spermatozoa and is required to render them competent to fertilize an oocyte. This step is a biochemical event; the sperm move normally and look mature prior to capacitation.
System and Method for Budgeting, Planning, and Supply Chain Management
InactiveUS20080065437A1Avoids missed profit opportunityReducing total prior art administrative costMarket predictionsResourcesSpermatozoa CapacitationSystems engineering
A system and method for optimizing planned profitability and network design. The system integrates sales and marketing planning and budgeting with the supply chain. This integration is achieved by inclusion of data representative of non-physical sales, general, and administrative (SG&A) costs, by development of response curves including sales and marketing SG&A costs, and by development of capacitation cost curves including nonphysical SG&A costs and supply chain costs. The optimality of the plan according to the present invention is made possible by applying optimization techniques to the integrated data. The optimality of the network design according to the present invention is made possible by making demands available for optimization.
Owner:DYBVIG CONSULTING
Method of Determining Sperm Capacitation
ActiveUS20100248302A1Evaluate effectPreparing sample for investigationBiological material analysisGangliosideCryoprotective Agent
This invention describes unique patterns of distribution of ganglioside GM1 in non-capacitated sperm and demonstrates that the pattern of distribution of GM1 undergoes changes that can be correlated with the process of capacitation and / or with acrosomal exocytosis. Accordingly, the present invention discloses a method for determining the ability of sperm to respond to capacitation and / or acrosomal exocytosis stimuli. The method comprises determination of distribution pattern for GM1. The method can be used for both diagnostic and predictive purposes when assessing male reproductive fitness, and can also be used to assess the effects of any agent or environment on sperm including cryoprotective agents and protocols, and contraceptive agents.
Owner:CORNELL UNIVERSITY +1
Method for pig in-vitro fertilization and embryo transplantation
The invention discloses a method for pig in-vitro fertilization and embryo transplantation, which comprises the following steps: oocyte preparation; sperm capacitation; adjustment of sperm concentration through adding sperm receiving fluid and capacitation through placing the adjusted mixture into an incubator; in-vitro fertilization and in-vitro oosperm culture; and embryo transplantation which adopts an embryo transplantation tube for transplantation. The method for pig in-vitro fertilization and embryo transplantation provided by the invention can be operated simply, the in-vitro fertilization and embryo transplantation can be realized without surgery, no damage can be caused to the pig, the success rate is high, the cost is low, and the method has great significance for promotion of pig in-vitro fertilization, pig cloning and transgenic embryo research and production.
Owner:WENS FOOD GRP CO LTD +1
Method of determining sperm capacitation
ActiveUS7160676B2Microbiological testing/measurementDead animal preservationDistribution patternAcrosome exocytosis
Owner:CORNELL RES FOUNDATION INC +1
Method of determining sperm capacitation
ActiveUS20070105188A1Microbiological testing/measurementPreparing sample for investigationObstetricsPhysiology
This invention describes unique patterns of distribution of ganglioside GM1 in non-capacitated sperm and demonstrates that the pattern of distribution of GM1 undergoes changes that can be correlated with the process of capacitation and / or with acrosomal exocytosis. Accordingly, the present invention discloses a method for determining the ability of sperm to respond to capacitation and / or acrosomal exocytosis stimuli. The method comprises determination of distribution pattern for GM1. The method can be used for both diagnostic and predictive purposes when assessing male reproductive fitness, and can also be used to assess the effects on sperm of cryoprotective agents and protocols, and contraceptive agents.
Owner:CORNELL RES FOUNDATION INC +1
Two-layer percoll density gradient centrifugal separation method of boar sperms
InactiveCN102250836ASimple processing methodEasy to handleGerm cellsAnti-sperm antibodySemen liquefaction
The invention provides a two-layer percoll density gradient centrifugal separation method of boar sperms. The method is suitable for normal sperm specimen and sperm specimen with low sperm density or low sperm motility, and is a simple, convenient and fast sperm treatment method. The method can be used for remove leucocytes, bacteria, fragments and anti-sperm antibodies in the sperms and improving the delayed sperm liquefaction, the sperm in-vitro capacitation and the like, so that the sperms can effectively fertilize ova to improve the pregnancy rate, and the percentage of the sperms with motility and normal shape in the obtained sperms is about 95%. The method provided by the invention is suitable for screening sperms with motility from fresh boar sperms and diluted sperms, thus providing high-quality sperms for the study of boar sperm capacitation mechanism and in-vitro fertilization.
Owner:JILIN UNIV
Method for improving moveability of sperm in frozen bull semen and in vitro fertilization rate
The invention provides a method for improving the moveability of sperm in frozen bull semen and the in vitro fertilization rate. The method is characterized in that when SP-TALP washing liquid is used for washing at a prior disposal stage of the frozen bull semen, or when PHE (phenylalanine) capacitation liquid is used for capacitation disposal at a capacitation disposal stage of the semen before fertilization, or when fertilization drips are prepared at an in vitro fertilization stage, glutathione and caffeine of which the purities are both higher than 98% are added; in the fertilization process of each piece of frozen bull semen, in the SP-TALP washing liquid containing bull semen or in the mixed liquid of IVF-TALP seminal fluid containing the bull semen and the PHE capacitation liquid or in the mixed liquid of the fertilization drips containing oocytes and the bull semen, the final concentration of the glutathione and the caffeine is 5mmol / L. According to the method disclosed by the invention, two substances are jointed to be respectively applied to three stages in the fertilization process of the frozen bull semen, the moveability of the sperm is greatly improved, and the fertilization rate of the sperm is improved.
Owner:武汉市畜牧兽医科学研究所
Cryoprotectant and application thereof, sperm refrigerating fluid and preparation method of sperm refrigerating fluid
InactiveCN111149795AImprove fertilizationImprove productivity functionDead animal preservationSucrosePhysiology
The invention relates to the technical field of assisted reproduction, and discloses a cryoprotectant and an application thereof, a sperm refrigerating fluid and a preparation method of the sperm refrigerating fluid. The sperm refrigerating fluid comprises a cryoprotectant, a composition and a basic culture solution, wherein the cryoprotectant comprises 0.3 to 1.1 mg / L of paclitaxel, 16 to 22 g / Lof trehalose, 7 to 13 g / L of cane sugar and 80 to 250 mL / L of glycerinum; the composition comprises 12 to 26 mg / L of resveratrol, 0.2 to 1.9 mg / L of reduced glutathione, 436 to 775 mg / L of lecithin, 0.5 to 1.6 mg / L of cholesterol, 9 to 17 mg / L of ATP sodium salt, 11 to 28 mg / L of heparin, 93 to 299 mg / L of phosphatidylserine and 5 to 25 mg / L of coenzyme Q10. The sperm refrigerating fluid can reduce damage of sperms in low-temperature freezing and freezing resuscitation processes, improve vitality of the sperms after freezing resuscitation, prolong survival time of the sperms after freezing resuscitation, promote sperm capacitation and enhance penetrating fertilization capacity of the sperms to egg cells. Thus, the fertilization success rate and blastocyst formation rate of the ova are improved, and finally the effect of improving the pregnancy success rate of the in-vitro fertilization assisted reproduction technology is achieved.
Owner:成都艾伟孚生物科技有限公司
Identifying status of male fertility by determining sperm capacitation
This disclosure provides a method for determining male fertility status. The method comprises determining GM1 distribution patterns following induced sperm capacitation, identifying the frequency of distribution of various patterns, and determining if the frequency distribution of certain GM1 patterns in response to induced capacitation is altered. Based on the change in the frequency distribution patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.
Owner:CORNELL UNIVERSITY
Protein capable of adjusting sperm capacitation and use thereof
The invention belongs to the biotechnology field and discloses a use of an epididymis specific expression gene HongrES1 or an agonist thereof or an antagonist in the preparation of compositions for regulating sperm capacitation of mammals; the invention further discloses a method for screening effective substances for regulating the sperm capacitation of the mammals by taking the epididymis specific expression gene as a target point. The invention firstly reveals that the HongrES1 has an important role on the aspect of regulating the sperm capacitation and the fertility of the mammals, thereby providing the effective target point for researching male reproduction.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
Crisp polypeptides as contraceptives and inhibitors of sperm capacitation
InactiveUS20050282729A1Inhibit capacitationAvoid reactionPeptide/protein ingredientsDepsipeptidesPhosphorylation InhibitionBiology
Included in the present invention are methods of inhibiting sperm capacitation, inhibiting the phosphorylation of a protein at tyrosine residues, inhibiting an acrosomal reaction, and inhibiting fertilization of an egg by sperm with the administration of a CRISP polypeptide.
Owner:MINNESOTA RGT UNIV OF A CORP OF MN
Methods and test kits for determining male fertility status
This disclosure provides a method for determining male fertility status, and its relationship to the probability of generating a pregnancy as calculated using a regression model. The method comprises determining GM1 localization patterns following induced sperm capacitation, identifying the percentage of various patterns, particularly the ratio of [(AA+APM) / total number of GM1 localization patterns] and determining if the percentage of certain GMI localization patterns in response to induced capacitation is altered. Based on the change in the percentage of localization patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.
Owner:ANDROVIA LIFE SCI LLC
Method for lamb oocyte in-vitro fertilization culture
The invention belongs to the field of biology, and particularly relates to a method for lamb oocyte in-vitro fertilization culture. The method comprises the steps of oocyte acquisition and maturation culture, sperm capacitation, mature oocyte in-vitro fertilization and zygote culture. During sperm capacitation and mature oocyte in-vitro fertilization, incubating time, the rotating speed of a centrifugal machine, centrifugation time, the number of times of centrifugal washing and mature oocyte culture time conditions are optimized, so that the zygote cleavage rate of lambs is basically the same as that of adult ewes.
Owner:YANGZHOU UNIV
Hydrogen ion enriched acidic conservation contraception gel for department of gynecology and preparation method of hydrogen ion enriched acidic conservation contraception gel
InactiveCN110623919AIncrease concentrationEnsuring healthy growth and reproductionAerosol deliveryOintment deliveryDiseaseSide effect
The invention provides hydrogen ion enriched acidic conservation contraception gel for department of gynecology and a preparation method of the hydrogen ion enriched acidic conservation contraceptiongel, and relates to the technical field of medical health. The preparation method comprises the steps of performing wetting and dispersing on an ammonium acryloyldimethyltaurate / VP copolymer with glycerin or propylene glycol to form paste, mixing the paste with a saturated hydrogen-enriched aqueous solution, and performing standing until the ammonium acryloyldimethyltaurate / VP copolymer presents expansional state, so as to obtain a glue solution; mixing lactic acid with glycollicacid, citric acid, lactein, calcium propionate, ferment, active VC, glutathione GSH and the saturated hydrogen-enriched aqueous solution to obtain a mixed solution; and then uniformly mixing the mixed solution with the glue solution, and adjusting the pH value to 3.8-4.0 so as to obtain the hydrogen ion enriched acidic conservation contraception gel. The hydrogen ion enriched acidic conservation contraception gel disclosed by the invention does not destroy beneficial bacteria in the vagina, and does not have any side effects; the pH value of products is completely consistent with the normal pH value of the vagina; the vagina mucosa can be urged to be young, and the disease resistance can be improved; and the hydrogen ion enriched acidic conservation contraception gel can restrain breeding of pathogenic bacteria, prevent vagina infection, restrain capacitation and sperm activity, and exert the contraception effects.
Owner:江苏万可瑞科技实业有限公司
Vaginal nutrient solution used for improving sperm fertilizing ability and further improving natural fertilization rate and preparation method of vaginal nutrient solution
InactiveCN107308187AStable in natureNon-irritatingPeptide/protein ingredientsHydroxy compound active ingredientsSodium bicarbonateSodium lactate
The invention provides a vaginal nutrient solution used for improving sperm fertilizing ability and further improving a natural fertilization rate and a preparation method of the vaginal nutrient solution. The vaginal nutrient solution disclosed by the invention comprises the following raw material components: fructose, vitamin E, zinc sulfate, sodium chloride, potassium chloride, sodium dihydrogen phosphate, sodium lactate, 4-(2-hydroxyerhyl)piperazine-1-erhaesulfonic acid and / or sodium 4-(2-hydroxyerhyl)piperazine-1-erhaesulfonate, sodium pyruvate, sorbitol, sodium bicarbonate, polyvinyl alcohol, tetrasodium ethylenediamine tetraacetate and / or phenol red, magnesium chloride, calcium chloride and the like. The sperm survival time can be prolonged, the sperm mobility is enhanced, and capacitation of sperms is promoted, so that the quantity and quality of sperms at sites of fertilization are improved, and the pregnancy probability during actual intercourse is increased. The vaginal nutrient solution disclosed by the invention has the advantages of direct action sites, strong action effect and pertinence and the like. Meanwhile, the method disclosed by the invention is simple and convenient to prepare, no large instrument or equipment is needed, and the method is applicable to large-scale industrial production.
Owner:冯浩
Identifying status of male fertility by determining sperm capacitation and companion collection kit
PendingUS20200141957A1Improve toleranceExtended storage timePreparing sample for investigationBiological material analysisObstetricsPhysiology
Owner:ANDROVIA LIFE SCI LLC
Method for obtaining sterile mammal with rapidness and high efficiency
The invention provides a method for obtaining a sterile mammal with rapidness and high efficiency. The method comprises the following steps: a) obtaining a sperm of a male mammal, and subjecting the obtained sperm to capacitation; b) obtaining an ovum of a female mammal; c) preparing an oosperm by utilizing the sperm obtained in the step a) and the ovum obtained in the step b); d) obtaining an aspermous male mammal; e) preparing a pseudopregnant female mammal; f) preparing an embryo by utilizing the oosperm obtained in the step c); g) obtaining a pseudopregnant female mammal containing the embryo obtained in the step f); and h) subjecting the female mammal obtained in the step g) to sterile culture so as to obtain the sterile mammal. The method provided by the invention has the advantages of short time, fast action, high efficiency and small breeding population, so the sterile mammal used for various purposes can be provided with rapidness and high efficiency.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
Composition for promoting fertilization of frozen semen of mouse
InactiveCN111117950AImprove survival rateIncrease mobilityCulture processCell culture active agentsPhysiologyGene Modification
The invention discloses a composition for promoting fertilization of frozen semen of a mouse. 0.01 mg / ml PVA and 0.75 mM methyl-beta-cyclic dextrin (MBCD) are added to an m HTF solution to serve as asperm capacitation solution; and 1.0 mM reduced glutathione is added to an m HTF solution to serve as a sperm in-vitro fertilization solution. The sperm capacitation solution used in the invention canremarkably protect the integrity of a plasma membrane and an acrosome, reduce the influence of ROS, and prominently improve survival rate and mobility of a sperm; and in combination with the in-vitrofertilization solution containing the reduced glutathione in the invention, an in-vitro fertilization success rate of a frozen-thawed sperm of a genetically modified mouse with an inbred strain C57BL / 6J as a background can be improved remarkably, and a two-cell developmental rate after fertilization is 60-65%.
Owner:SUN YAT SEN UNIV
Regulation of Sperm Function
InactiveUS20070275366A1Reduce exerciseIncrease in motilityCell receptors/surface-antigens/surface-determinantsAngiotensinsCell-Extracellular MatrixMotility
An extracellular matrix protein, such as fibronectin, vitronectin or laminin, is added to a sperm sample to bring the sperm into a low motility, non-capacitated state or maintain a pre-existing non-capacitated state. Subsequently, at an appropriate time in an in vitro fertilization study or in vivo fertilization procedure, angiotensin II or a related peptide is added to the sperm sample to enhance motility and capacitate the sperm. The extracellular matrix protein prevents undesirable early natural capacitation, and artificial insemination procedures can be made more precise, as sperm can be brought to the right state of capacitation for transfer at precisely the right time. The treatment with angiotensin II can be combined with use of the peptide RGD to compete with the binding of the extracellular matrix protein and so suppress its effect.
Owner:QUEEN MARY UNIV OF LONDON
A method for capacitation of yak sperm in vitro
InactiveCN103173406BEase of production and use in vitroOperation diversificationMicrobiological testing/measurementGerm cellsEmbryoSemen
Owner:LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
Sperm fertility capacity test and sperm decapacitating supplement
Disclosed herein are methods for determining the fertility of a sperm sample or a source of a sperm sample; the methods comprising labeling the sperm sample with a zinc probe, identifying presence and / or localization of zinc associated with spermatozoa in the sample and determining the fertility of the sample based on the zinc presence and / or localization in the spermatozoa as compared to a reference pattern associated with non-capacitated spermatozoa or a reference pattern associated with sperm capacitation. Also disclosed are methods of improving sperm fertility and / or decreasing premature capacitation while handling, storing, or transporting semen, the methods comprising adding exogenous zinc to a sperm sample or semen sample. Also disclosed are compositions for improving sperm fertility and / or reducing premature capacitation, the compositions comprising exogenous zinc ions.
Owner:SUTOVSKY PETER +3
A method for detecting sperm acrosome reaction
ActiveCN111733203BShorten the timeImprove efficiencyMicroorganismsMicrobiological testing/measurementStainingPhysiology
Owner:青岛复诺生物医疗有限公司
A kind of bovine sperm capacitation liquid and sperm capacitation method in vitro
InactiveCN106520680BMaintain the effect of external capacitationGuaranteed completenessGerm cellsSperm plasma membraneSperm motility
Owner:HENAN UNIV OF SCI & TECH
Bovine sperm treatment method and bovine intracytoplasmic sperm injection method
The invention relates to a bovine sperm treatment method and a bovine intracytoplasmic sperm injection method, and belongs to the technical field of intracytoplasmic sperm injection. According to the bovine sperm treatment method, firstly, the sperm is treated by using a weakly alkaline solution, and therefore, the tail part of the sperm is shortened by 1 / 3-1 / 2; and then, the sperm of which the tail part is shortened by 1 / 3-1 / 2 is selected and is injected into the oocytes. After the bovine sperm treatment method disclosed by the invention is adopted for carrying out the treatment, only the tail part of the sperm is shortened by 1 / 3-1 / 2, the sperm still retains the centrosome, the integrity of the sperm is structurally retained, the method is more similar to the change of sperm capacitation in a natural fertilization process, the embryonic development potential is improved, and the development rate of microinjection embryos is improved. In addition, the tail part of the sperm treated by the bovine sperm treatment method is shortened by 1 / 3-1 / 2, and shortening of the tail part can lower the difficulty of microinjection, improve the operation speed of injection and improve the efficiency of intracytoplasmic sperm injection.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE HENAN ACAD OF AGRI SCI
Method for preparing activated carbon from coal-to-oil industrial waste
InactiveCN109626372ALow costHigh specific capacityMaterial nanotechnologyCarbon compoundsWater vaporBiological activation
The invention provides a method for preparing activated carbon through coal-to-oil industrial waste. The method comprises the following steps that firstly, the coal-to-oil industrial waste is pretreated, then subjected to vapor activation, subjected to impurity removal and dried at last to obtain super capacitation activated carbon. The pore structure of the super capacitation activated carbon prepared through the method is developed, the capacity of the activated carbon can reach 300 F / g or above when the current density is 0.5 A / g, and after 5,000-time charging and discharging, the capacityretention ratio of the activated carbon can reach 90% or above.
Owner:仲兴行(北京)科技有限公司
Methods and test kits for determining male fertility status
This disclosure provides a method for determining male fertility status. The method comprises determining GM1 localization patterns following induced sperm capacitation, identifying the percentage of various patterns, particularly the ratio of [(AA+APM) / total number of GM1 localization patterns] and determining if the percentage of certain GM1 localization patterns in response to induced capacitation is altered. Based on the change in the percentage of localization patterns of certain patterns in response to induced capacitation, alone or in combination with other sperm attributes, male fertility status can be identified.
Owner:ANDROVIA LIFE SCI LLC
A kit for evaluating sperm quality after in vitro capacitation and using method thereof
ActiveCN105779568BReduce typesReduce experiment costMicrobiological testing/measurementSodium acetateSialidase
Provided are a kit for evaluating the sperm quality after in vitro capacitation and a method of use thereof. The kit comprises a detection buffer 1, a BWW culture solution, a solution for in vitro capacitation, a fluorescent reagent, a sialidase standard stock solution and a detection buffer 2. The detection buffer 1 is a phosphate buffer or a BWW culture solution. The detection buffer 2 is a phosphate buffer containing 0.05 mmol / l of sodium acetate and 0.25 μg / μl of bovine serum albumin.
Owner:成都思瑞迪医疗科技有限公司
Regulation of sperm function
InactiveCN1893968AReduced exercise capacityPeptide/protein ingredientsMammal material medical ingredientsCell-Extracellular MatrixMotility
An extracellular matrix protein, such as fibronectin, vitronectin or laminin, is added to a sperm sample to bring the sperm into a low motility, non-capacitated state or maintain a pre-existing non-capacitated state. Subsequently, at an appropriate time in an in vitro fertilization study or in vivo fertilization procedure, angiotensin II or a related peptide is added to the sperm sample to enhance motility and capacitate the sperm. The extracellular matrix protein prevents undesirable early natural capacitation, and artificial insemination procedures can be made more precise, as sperm can be brought to the right state of capacitation for transfer at precisely the right time. The treatment with angiotensin II can be combined with use of the peptide RGD to compete with the binding of the extracellular matrix protein and so suppress its effect.
Owner:QUEEN MARY UNIV OF LONDON
Method for improving sperm motility and increasing in-vitro-fertilization rate of frozen bovine semen by aid of genistein
ActiveCN103462724BImprove athletic abilityImprove fertilization rateAnimal reproductionSerum phosphateGenistein
Owner:武汉市畜牧兽医科学研究所
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