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The application discloses a method for detecting N glycosylation of an Fc fragment of an anti-MDA5 antibody, and is characterized in that the method comprises the following steps: step one, an anti-MDA5 DSIgG separation step; step two, a sample glycosylation treatment step; step three, an on-machine detection step; and step four, a data analysis step. The application also discloses uses thereof. The application directly captures anti-MDA5 disease-specific IgG through recombinant MDA5-Flag magnetic beads, so that the interference of normal IgG is excluded.