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Method for detecting fc fragment of anti-mda5 antibody n-glycosylation and use thereof

PendingCN122385878AEasy to typeAchieve high sensitivity detection
The application discloses a method for detecting N glycosylation of an Fc fragment of an anti-MDA5 antibody, and is characterized in that the method comprises the following steps: step one, an anti-MDA5 DSIgG separation step; step two, a sample glycosylation treatment step; step three, an on-machine detection step; and step four, a data analysis step. The application also discloses uses thereof. The application directly captures anti-MDA5 disease-specific IgG through recombinant MDA5-Flag magnetic beads, so that the interference of normal IgG is excluded.
Owner:SHANGHAI CITY JIADING DISTRICT CENT HOSPITAL

Drilling device for mining machinery

The utility model discloses a mining machinery drilling device which comprises a drill rod and a drill bit, the lower end of the drill rod is fixedly connected with an expansion head, the drill bit is sleeved on the expansion head, and an insertion block is inserted in the lower portion of the expansion head. A crowbar is matched with the lower part of the inserting block. And a slot is formed in the expansion head. The inserting block comprises an inserting rod, the side portion of the inserting rod is fixedly connected with a mud blocking piece, and the lower end of the inserting rod is fixedly connected with a stopping block. The insertion block further comprises a guide head which is fixedly connected to the upper end of the insertion rod. The inserting block further comprises a guide strip which is fixedly connected to the upper end of the mud blocking piece. The crowbar comprises a bar body, after a drill bit is arranged on an expansion head in a sleeving mode, an insertion block is plugged into the expansion head, then the insertion block is driven into the expansion head through a hammer, the insertion block acts on the expansion head outwards, the expansion head expands outwards in a slight amplitude mode, and therefore the expansion head and the drill bit are fixed, and the expansion head and the drill bit are fixed in a slight expansion mode of the expansion head. The drill mounting difficulty is reduced.
Owner:韩路新