The present application belongs to the technical field of
glycosylation detection and analysis, and particularly relates to a serum
glycosylation flow
analysis method based on
lectin affinity. The present application enriches target molecules in a sample to be measured by
coupling a
polystyrene microsphere with a
decoy molecule, and then incubates the
polystyrene microsphere with a fluorescently labeled
lectin, and directly analyzes the
glycosylation information of the target molecules by a flow cytometer. In the present application, the
decoy molecule of the
target protein is coupled to the
polystyrene microsphere to enrich the
target protein, and the
target protein is focused for subsequent analysis, which can effectively reduce the
field of view and improve the specificity. After the
lectin is combined with the target
protein, the
fluorescence analysis of the
flow cytometry is directly performed, which can effectively reduce the damage of the washing operation to the affinity of the lectin, and thus overcomes the
weakness of the weak affinity between the lectin and the
sugar chain. Moreover, only a small amount of sample is required to realize the analysis of multiple lectins, and the washing-free real-time analysis can be realized, which provides a new way for the analysis of glycosylation by using lectin, and has important potential application value.