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Method and composition for repairing heart tissue

A technique for cardiac tissue, composition, applied in the field of compositions for repair and/or regeneration, capable of solving problems such as lack of cell expansion

Inactive Publication Date: 2008-04-23
REGENETECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Boyse's disclosure lacks cell expansion for therapeutic applications

Method used

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  • Method and composition for repairing heart tissue
  • Method and composition for repairing heart tissue
  • Method and composition for repairing heart tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Example 1 - Practical TVEMF Expansion of Cells in a TVEMF Bioreactor

[0100] Peripheral blood was collected and peripheral blood cells expanded as indicated in Table 1, described below.

[0101] A) Harvesting and maintaining cells

[0102] Human peripheral blood (75 ml; approximately 0.75 × 10 6 cells / ml), blood collected from 10 donors was suspended in 75 ml of Iscove's Modified Dulbecco's medium (IMDM) (GIBCO, Grand Island, NY) supplemented with 20% 5% human albumin (HA), 100 ng / ml recombinant human G-CSF (Amgen Inc., Thousand Oaks, CA) and 100 ng / ml recombinant human stem cell factor (SCF) (Amgen). A portion of each blood sample was retained as a "control" sample. The peripheral blood mixture was placed in a TVEMF bioreactor as shown in Figures 2 and 3 herein. TVEMF amplification at 37 °C, 6% CO 2 and O in normal air 2 / N ratio. The TVEMF bioreactor was initially spun at 10 revolutions per minute (rpm), then adjusted as needed to keep peripheral blood cells s...

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Abstract

The present invention is directed to the TVEMF-expansion of mammalian blood stem cells, preferably CD34+ / CD38- cells, to compositions resulting from the TVEMF-expanded cells, and to a method of treating heart disease or repairing heart tissue with the compositions.

Description

field of invention [0001] The present invention relates to methods of repairing and / or regenerating cardiac tissue and compositions for effecting such repairing and / or regeneration. Background of the invention [0002] Regeneration of cardiac tissue in mammals, especially humans, has long been a desire of the medical community. For some tissues, most of the repair of human tissue is achieved by transplanting similar tissues from donors. Tissue transplantation basically started with a kidney transplant from one of the Herrick twins to the other, and later, South African doctor Christian Barnard transplanted Denise Darval's heart to Louis Washkansky on December 3, 1967, making the technique world famous , and since then tissue transplantation has become a widely accepted method for prolonging the life of terminally ill patients. [0003] Human tissue transplantation has encountered major problems since its initial application, mainly due to tissue rejection by the body's nat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19C12N5/08A61K35/14A61K35/12C12N5/0789
CPCA61K2035/124C12N2501/22A61K38/193C12N5/0647A61P9/00A61K2300/00A61K35/14
Inventor 唐尼·拉德
Owner REGENETECH INC
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